OBJECTIVES:Examination of the regulation of the human sperm acrosome reaction as an example of ligand-induced exocytosis.METHODS:Development of monoclonal antibodies (mab) against immunaffinity-purified sperm antigen SAA-1. Examination of cross-reactivity of the mabs to human tissues using immunohistochemistry. Examination of reactivity to endometrial carcinoma cell lines and to PC-12 cells by immunohistochemistry and by radioimmunoassay.RESULTS:Mabs to immunoaffinity-purified SAA-1 recognize three coprecipitating molecules. Cross-reactivity was demonstrated to glandular epithelia exhibiting ligand-induced exocytosis, and to endometrial carcinoma and PC-12 cells.CONCLUSIONS:The proteins described could be components of the exocytosis-regulating machinery of human spermatozoa.
Examination of the regulation of the human sperm acrosome reaction as an example of ligand-induced exocytosis.Development of monoclonal antibodies (mab) against immunaffinity-purified sperm antigen SAA-1. Examination of cross-reactivity of the mabs to human tissues using immunohistochemistry. Examination of reactivity to endometrial carcinoma cell lines and to PC-12 cells by immunohistochemistry and by radioimmunoassay.Mabs to immunoaffinity-purified SAA-1 recognize three coprecipitating molecules. Cross-reactivity was demonstrated to glandular epithelia exhibiting ligand-induced exocytosis, and to endometrial carcinoma and PC-12 cells.The proteins described could be components of the exocytosis-regulating machinery of human spermatozoa.
Objectives: Examination of the regulation of the human sperm acrosome reaction as an example of ligand-induced exocytosis. Methods: Development of monoclonal antibodies (mab) against immunaffinity-purified sperm antigen SAA-1. Examination of cross-reactivity of the mabs to human tissues using immunohistochemistry. Examination of reactivity to endometrial carcinoma cell lines and to PC-12 cells by immunohistochemistry and by radioimmunoassay. Results: Mabs to immunoaffinity-purified SAA-1 recognize three coprecipitating molecules. Corss-reactivity was demonstrated to glandular epithelia exhibiting ligand-induced exocytosis, and to endometrial carcinoma and PC-12 cells. Conclusions: The proteins described could be components of the exocytosis-regulating machinery of human spermatozoa.
Antje Keberle合作论文数6Grayson B. Lipford合作论文数Institute of Medical Microbiology and Hygiene, Technical University of Munich6Veronika Schlamp合作论文数6G Kuhn合作论文数5Cosima Brucker合作论文数5P. Berger合作论文数3E. Melchert合作论文数3B. Föst合作论文数3G. Büge合作论文数3C. Larcher合作论文数3T. Nebe合作论文数3K. Maag合作论文数3
Natasya Ruth合作论文数3B Hüneke合作论文数3G. Pirschner合作论文数3T Strohmeyer合作论文数3I Tossounidis合作论文数3W G Rossmanith合作论文数3Angela Reles合作论文数3T Cunze合作论文数3C. Marth合作论文数3B. Wartusch合作论文数3Thomas Reissmann合作论文数3Bu Sevin合作论文数3A. von Daimling合作论文数3R. Höpfl合作论文数3C Brumm合作论文数3C Zoll合作论文数3L Pache合作论文数3Frank M合作论文数3Meden H合作论文数3Hans-Peter Horny合作论文数3
Wulf Paschen合作论文数3Andreas Luttkus合作论文数3Anke Homann合作论文数3Babur Aydeniz合作论文数3Klaus Marzusch合作论文数3I. Pündmann合作论文数3Joachim Gnirs合作论文数3Bogdan Djuricic合作论文数3Andrea Steinbron合作论文数3Walter Nathrath合作论文数3Doris Macchiella合作论文数3Mila Cervar合作论文数3
Ulf Janssen合作论文数3Petra Ziffer合作论文数3C Rybakowski合作论文数3A. Zeimet合作论文数3A. Lopens合作论文数3
