Premature ovarian failure(POF)is a gynecological endocrine disease caused by ovarian dysfunction.Mesenchymal stem cells(MSCs)can effectively treat premature ovarian failure,and play a role in repairing ovarian structure and improving ovarian function.The specific mechanism can be a direct differentiation into granulosa cells or inhibition of their apoptosis through the homing effect of MSCs or differentiate into primordial germ cells.Benefit of MSCs can also be attributed to ovarian angiogenesis promotion,immune regulation,reduce oxidative stress re-sponse and paracrine,all potentially promote recovery of ovarian function,and thus improve the fertility of women of childbearing age.

目的 观察参芪通脉方防治糖尿病肾病(DN)患者自体动静脉内瘘(AVF)早期失功的疗效及对纤溶系统功能、脂质代谢与转化生长因子-β1(TGF-β1)的影响.方法 将2017年3月—2019年3月在内蒙古医科大学附属医院接受AVF血液透析的86例终末期DN患者随机分为2组,对照组43例于内瘘成熟首次透析后给予阿司匹林干预,观察组43例在对照组基础上给予参芪通脉方干预,疗程均为12周.记录2组AVF早期失功发生率,观察2组AVF使用前及使用6周、12周后内瘘血流速度与内瘘静脉血管内径、纤溶指标、脂代谢指标与血清TGF-β1的变化.结果 观察组AVF使用12周AVF早期失功发生率为16.3％(7/43),对照组为32.6％(14/43),观察组明显低于对照组(P<0.05).与AVF使用前比较,2组AVF使用6周、12周后内瘘血流速度均减慢(P均<0.05),内瘘静脉血管内径均缩短(P<0.05),但观察组上述指标均优于对照组(P均<0.05).与AVF使用前比较,2组AVF使用6周、12周后的纤维蛋白原(FIB)、D-二聚体水平均明显增高(P均<0.05),但观察组上述时间的FIB、D-二聚体水平均明显低于对照组(P均<0.05).与AVF使用前比较,2组AVF使用6周、12周后的三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、TGF-β1水平均明显增高(P均<0.05),高密度脂蛋白胆固醇(HDL-C)水平均明显降低(P均<0.05),但观察组上述时间的TG、TGF-β1水平均明显低于对照组(P均<0.05),HDL-C水平高于对照组(P<0.05).结论 参芪通脉方可显著降低DN患者AVF早期失功发生率,有效维持AVF功能,机制可能与调节纤溶功能、脂质代谢紊乱及TGF-β1表达水平有关.

ObjectiveTo retrospectively investigate the effect of combined treatment with human umbilical cord blood mononuclear cells (hUCB-MNCs) and human umbilical cord mesenchymal stem cells (hUC-MSCs) on liver function, inflammation grade, and immune function in patients with hepatitis B-related decompensated cirrhosis. Methods 11 patients with liver cirrhosis who were admitted to the Affiliated Hospital of Inner Mongolia Medical University from November 2016 to June 2019 were enrolled in this clinical study and were given infusion of hUCB-MNCs (＞18×109/time) once in the first week and infusion of hUC-MSCs (1×106/kg) once a week in the second, third, and fourth weeks. Reexamination was performed at 4, 8, and 12 weeks after treatment to compare the changes in liver function, blood ammonia, blood coagulation factors, serum levels of cytokines, and lymphocyte subsets after treatment, and the changes in neurological and psychiatric symptoms were observed and recorded. A one-way repeated-measures analysis of variance was used for comparison of continuous data between groups. ResultsAfter the combined treatment with hUCB-MNCs and hUC-MSCs, all 11 patients achieved certain improvements in psychiatric and neurological symptoms after infusion, and liver function parameters and blood coagulation function basically returned to normal (all P＜0.05). At 12 weeks after the combined infusion of cells, blood ammonia level returned to the normal level (P＜0.05), and there were significant reductions in the levels of the inflammatory cytokines interleukin-6 and tumor necrosis factor (F=49497 and 37.071, both P＜0.05) and significant increases in the levels of the anti-inflammatory cytokines transforming growth factor-β and interleukin-10 (F=35.843 and 15.918, both P＜005). There were also significant reductions in the percentages of the cytotoxic cells CD3+CD8+ T and CD19+ B cells (F=52.242 and 89.097, both P＜0.05) and a significant increase in the regulatory T cells CD4+CD25+ (F=17.337, P＜0.05). ConclusionCombined treatment with hUCB-MNCs and hUC-MSCs for liver cirrhosis can optimize liver function, reduce the production of inflammatory cytokines, alleviate liver inflammation and liver cell destruction, and increase regulatory T cells, thereby affecting the body’s immune function.

目的 观察温肾补脾生血方联合左卡尼汀治疗维持性血液透析(MHD)伴促红细胞生成素(EPO)低反应性的疗效及对患者血清维生素D、叶酸、维生素B12和炎症因子的影响.方法 将2016年1月—2019年1月内蒙古医科大学附属医院收治的60例MHD伴EPO低反应性患者随机分为2组:对照组30例给予MHD联合左卡尼汀治疗,观察组30例在对照组治疗基础上给予温肾补脾生血方治疗,疗程均为2个月.比较2组治疗前后血清总蛋白(TP)、白蛋白(ALB)、血红蛋白(Hb)、维生素D、叶酸、维生素B12、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)、C反应蛋白(CRP)水平,评价临床疗效及不良反应发生情况.结果 观察组和对照组总有效率分别为73.3％(22/30)和55.0％(16/30),观察组明显高于对照组(P<0.05).治疗后2组TP、ALB、Hb、维生素D、叶酸、维生素B12水平均明显增高(P均<0.05),IL-6、TNF-α、CRP水平均明显降低(P均<0.05),治疗后观察组以上指标均明显优于对照组(P均<0.05).观察组和对照组不良反应总发生率分别为16.7％(5/30)和36.7％(11/30),观察组明显低于对照组(P<0.05).结论 温肾补脾生血方联合左卡尼汀治疗可显著改善MHD伴EPO低反应性患者的营养状况,提高血清维生素D、叶酸、维生素B12水平,减轻炎症反应,减少不良反应发生.

目的:观察腺嘌呤诱导的慢性肾脏病(CKD)肾性骨病大鼠模型的腰椎及股骨的生物力学特点.方法:20只雄性SD大鼠分为正常对照组和CKD组.第6周末处死大鼠,行血清生化标志物检测,取股骨和第五腰椎做骨密度(BMD)及骨生物力学分析. 结果:在第6周末,CKD组大鼠血清尿素氮、血清肌酐、血清无机磷、血清甲状旁腺激素较正常对照组均明显升高,血清钙明显降低.与正常对照组比较,CKD组的股骨和第五腰椎椎体BMD均明显降低.骨生物力学,股骨三点弯曲实验结果提示,CKD组大鼠的股骨结构力学指标和材料力学指标均明显降低;腰椎压缩实验结果提示,CKD组大鼠腰椎的结构力学指标和材料力学指标均明显降低. 结论:腺嘌呤诱导的大鼠CKD模型能较好地模拟CKD时出现的高转运肾性骨病的生物力学特征,表现为皮质骨和松质骨材料力学和结构力学性能的下降,有望成为CKD肾性骨病模型的研究载体.

目的:评价脐带血来源单个核细胞(umbilical cord blood mononuclear cell,UCMC)诱导分化的免疫细胞对小细胞肺癌(small cell lung cancer,SCLC)患者免疫功能的影响.方法:选取2012年1月至2015年12月就诊于内蒙古医科大学附属医院的90例SCLC患者,采用分层随机方法分为对照组(45例,EP方案)和研究组(45例,EP方案+UCMC诱导分化的免疫细胞),研究组患者在化疗结束后3～5 d进行一个疗程的UCMC诱导分化的免疫细胞治疗[(1～3)×1010个细胞/疗程],每30 d为一个周期.治疗前及治疗结束后12周,采用流式细胞术检测患者外周血中T细胞亚群的变化及相关因子IFN-γ、IL-2、IL-10、TGF-β1的变化,评价患者生活质量和不良反应等.结果:研究组获得CR 15例、PR 25例、SD 5例,研究组T细胞亚群含量较对照组有明显增加(P＜0.01)、并且恢复正常范围的时间明显短于对照组(P＜0.05);80.9％的患者炎性细胞因子IFN-γ在血清中升至或高于正常范围,IL-10、TGF-β1较治疗前降低(P＜0.05);患者生活质量较对照组有显著提高.结论:UCMC诱导分化的免疫细胞可以促进SCLC患者化疗后免疫功能的恢复.

目的:探讨抑制HPIP基因表达对TGF-β1诱导的肾小管上皮细胞上皮-间充质转化(epithelial-mesenchymal transition,EMT)及凋亡的影响.方法:10 ng/mL的TGF-β1刺激HK-2细胞24,48 h,Western印迹法检测HPIP蛋白表达.将HK-2细胞随机分为空白组、TGF-β1组和TGF-β1+si-HPIP组,处理24 h后,Western印迹法检测HPIP,E-cadherin,α-SMA,N-cadherin,Snail,Twist,t-AKT,p-AKT和Bax蛋白表达;流式细胞术检测细胞凋亡率.结果:TGF-β1刺激HK-2细胞24,48 h后HPIP蛋白表达均显著高于对照组(0 h)(P＜0.05).TGF-β1组E-cadherin蛋白表达显著低于空白组,α-SMA,N-cadherin,Snail,Twist,p-AKT和Bax蛋白表达及细胞凋亡率均显著高于空白组(P＜0.05),而TGF-β1+si-HPIP组E-cadherin蛋白表达显著高于TGF-β1组,α-SMA,N-cadherin,Snail,Twist,p-AKT和Bax蛋白表达及细胞凋亡率均显著低于TGF-β1组(P＜0.05).结论:抑制HPIP基因表达可延缓肾小管上皮细胞EMT进程和降低细胞凋亡率,机制可能与下调PI3K/AKT信号有关.

Objective To study the changes in morphology , phenotypes and gene expression pro-files of dendritic cells (DCs) following treatment with Seabuckthorn flavones (SF).Methods DCs were treated with 200μg/ml of SF and then cultured for 7 days.Changes in the morphology of DCs were observed under light microscope .Flow cytometry was used to detect DC surface molecules .Total RNA was extracted to construct the library for digital gene expression profiling ( DGE ) .Differentially expressed genes were screened out and further analyzed by gene ontology ( GO) enrichment analysis and Kyoto encyclopedia of genes and genomes ( KEGG ) pathway enrichment analysis .Results Compared with control group , SF treatment significantly enhanced the expression of HLA-DR, CD80, CD83 and CD86 on DCs.A total of 355 differentially expressed genes were screened out by DGE , including 176 up-regulated genes and 179 down-regulated genes .GO enrichment was mainly involved in the regulation and development of the immune sys -tem and other biological processes .KEGG pathway analysis showed that the significantly enriched pathways were closely related to inflammation , the immune system, cancer and other diseases .Conclusion SF can promote the expression of DC co-stimulatory molecules and pro-mature molecules, and regulate the expres-sion of immunity-related genes such as CD11a, SLAMF6, LMCD1, TSC22D3 and IKZF3.

In recent years , bioimmunotherapies have been widely used in the clinical treatment of various malignant tumors,among which the tumor antigen loaded dendritic cells (DC) vaccine combined immunocompetent cells technology is the most mature and most widely used .DC are known to be the most potent antigen-presenting cells and play an important role in anti-tumor immunity.DC is closely related to the occurrence and development of tumor ,which greatly increases the survival rate of malignant tumor patients is greatly improved on the basis of combined traditional therapy .But the role of DC in the body and its characteristics are not entirely clear ,and further clarification of the role of DC in the tumor microenviron-ment,will provide reference for improving the effect of biological treatment .

Objectives . In order to enhance the immunity of cancer patients to prevent relapse or to prolong survival time, umbilical cord blood mononuclear cells (UCMCs) were transplanted to cancer patients. Patients and Methods . UCMCs were transfused to 63 immunocompromised gastrointestinal cancer patients with nonmyeloablative (NMA) conditioning regimen. Results . The clinical study showed that the number of both T and B cells increased much more rapidly after transfusion of UCMCs than that of the control group without transplantation (p<0.01). Proinflammation cytokines IFN γ and TNF α in serum increased to or above the normal range in 80.9% of patients at 12 weeks after UCMC transfusion. However, they recovered to the normal range in 21.7% of patients at the same time point in the control group only. In addition, the clinical investigation also showed that the transfusion of UCMC increased stable disease (SD) and reduced progressive disease (PD) significantly (p<0.01); however, it did not have significant effects on complete response (CR), partial response (PR), or mortality rates compared with the control group (p>0.05). Conclusions . UCMCs have powerful repairing effects on damaged cells and tissues and may reconstruct the impaired immunity. Transfusion of UCMCs could reconstruct the immunity of cancer patients with immunosuppression.

Objective To investigate the in vitro multi-direction differentiation ability of TERT gene modified sheep adi-pose derived mesenchymal stem cells and to verify the role of TERT gene in immortalization of sheep adipose derived mesenchymal stem cells. Methods The TERT-ADSCs were subcultured. P3 and P30 cells were selected to proceed induced osteogenic and neu-ral differentiation. Histological staining and RT-PCR of specifically expressed genes were used to identify the multi-direction differ-entiation ability of TERT-ADSCs. Results TERT-ADSCs can be stably subcultured to P30. After induction of osteogenic differenti-ation of P3 and P30,process of bone nodules was obviously visible,cells were stained reddish brown after alizarin red staining and specific gene CBFA and Osteocalcin were expressed. After induction of neural differentiation,cells presented triangle or dendritic morphology,Nissl bodies can be observed by toluidine blue staining and specific gene NSE and GFAP were expressed. Conclusion The TERT-ADSCs of sheep still has the potential of multi-direction differentiation after having been subcultured several times, which provides a theoretical basis for the immortalization study of ADSCs.

in recent years, our country people's economic income increased, the living standard has been greatly improved, the health, health problems become the people most concerned about one of the most hot topics, in the health inspection of the above consciousness fully play its active, people have entered the hospital for rehabilitation health inspection, health in-spection has become an important sector of the daily work of the hospital. However, due to the huge number of physical ex-amination, physical examination project more, resulting in a hospital medical work efficiency is low, the hospital staff bad treat check-up and other serious problems, the existence of these problems hinders our people demand of health examina-tion, hindering the medical industry of our country modernization.

As the one of main pharmacologically activity substance of Asragalus, Asragaloside Ⅳwas reported to have the immune regulation, anti-virus effect and anti-tumor effect. Meanwhile, it has distinguished pharmacologic actions. The effect of Asragaloside on Mesenchymal stem cell biological activity was reviewed for the first time. Recent studies show that Asragaloside Ⅳhas significant influence on the proliferation, differentiation, apoptosis and other biological activity of Mesenchymal stem cell. Its further research of pharmacological effects and mechanism of Asragaloside on stem cells provide the useful reference for clinical application.

BACKGROUND:Rabbit bone marrow mesenchymal stem cels as a kind of adult stem cels with strong proliferation and multilineage differentiation potential exhibit a tremendous application potential in tissue engineering and biological therapy. <br> OBJECTIVE:To in vitro culture, proliferate and identify rabbit bone marrow mesenchymal stem cels and to observe cel biological characteristics. <br> MEHTODS:Bone marrow of rabbits was extracted under sterile conditions to separate bone marrow mesenchymal stem cels using the whole bone marrow adherence method and Percol density gradient centrifugation method. Afterwards, the cels were purified and proliferated using differential adherence method. Morphology and growth pattern of cels were observed under microscope, and expression of cel surface antigen markers was detected by flow cytometry. <br> RESULTS AND CONCLUSION:Rabbit bone marrow mesenchymal stem cels presented with short adherent time and fast growth. After passage and purification, impurities cel counts were decreased. Primary cels presented with triangular, fusiform and spindly shapes. Passage 5 cels with single shape showed the typical polar swirling growth, and could not express CD34 and CD45, but expressed CD29 and CD44. These findings indicate that the cels cultured using the whole bone marrow adherence method and Percol density gradient&nbsp;centrifugation method possess stem cel characteristics in morphology, surface markers and multilineage differentiation, which have been identified as bone marrow mesenchymal stem cels by flow cytometry.

目的：综述黄芪甲苷免疫活性的研究进展。方法：检索、分析、总结近5年国内外文献，对黄芪甲苷抵抗病毒、免疫调节、抑制肿瘤的免疫活性进行了详尽的介绍与总结。结果与结论：黄芪甲苷具有抗病毒、提高免疫力、抑制肿瘤的作用。黄芪甲苷对干细胞的生物学行为的影响值得进行深入的研究。黄芪甲苷免疫活性的研究可为中药的开发应用、临床免疫疾病的治疗提供理论依据，为人类卫生健康做贡献。

BACKGROUND:As bone marrow mesenchymal stem cel s can be differentiated into osteoblasts under certain induction conditions, autologous bone marrow mesenchymal stem cel s can be implanted into the bone nonunion site of bone fracture. This new technology garners increasing attention of orthopedic clinicians. <br> OBJECTIVE:To summarize the clinical efficacy of transplantation of bone marrow mesenchymal stem cel s in the treatment of bone nonunion of limb fractures. <br> METHODS:A computer-based search of Foreign Medical Journal Ful-Text Service and CNKI databases was performed for articles related to bone marrow mesenchymal stem cel s for treatment of bone nonunion of limb fractures published from 1998 to 2014 using the keywords of“bone marrow stem cel s (BMSCs), stem cel transplantation (SCT), nonunions, tissue engineering”in English and Chinese, respectively. Literatures with repetitive content and lack of originality were excluded. A total of 36 literatures were obtained for further analysis. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s are transplanted into the end of bone nonunion, and then can be induced to differentiate into osteoblasts to repair bone nonunion and bone defects, <br> laying a theoretical basis for clinical application. Bone marrow mesenchymal stem cel s can repair bone defects, which provides an effective method and material to promote fracture healing. Transplantation of bone marrow mesenchymal stem cel s is safe and effective for treatment of bone nonunion of limb fracture.