Freshwater environments in polar regions, particularly in Antarctica, remain relatively underexplored regarding microbial biodiversity. In this study, we employed third-generation amplicon sequencing technology targeting full-length ribosomal ITS region to investigate the composition and diversity of fungal communities in 41 freshwater samples (lake water, stream water, and ice-melting water) collected from the Fildes Region in Antarctica. A total of 1,107,738 subsampled reads were obtained and classified into 1,884 operational taxonomic units (OTUs). Among these, 315 OTUs were assigned to Ascomycota, 156 to Basidiomycota, 624 to Chytridiomycota, 61 to Cryptomycota, 23 to Mucoromycota, 1 to Zoopagomycota, and 704 to unclassified fungal phyla. Common fungal genera identified included Phenoliferia, Metschnikowia, Cryolevonia, Linnemannia, and Pseudogymnoascus. Significant differences in fungal community composition were observed among different aquatic habitats. Furthermore, four environmental factors—pH, temperature, salinity, and electrical conductivity—showed significant correlations with the fungal community structure. These findings provide new insights into the fungal diversity of Antarctic freshwater ecosystems and advance our understanding of microbial life in extreme environments.

A Gram-stain-negative, non-motile, aerobic bacterium, designated as strain CPCC 100088T, was isolated from a rhizosphere soil sample of Kandelia candel in a mangrove. Growth of strain CPCC 100088T was observed at 15–37 °C, pH 6.0–9.0, and in the presence of 0–7

Rare actinomycetes, particularly Gordoni spp., are emerging as critical sources of bioactive metabolites and opportunistic pathogens. In this study, we isolated three novel Gordonia strains from soil samples and characterized their taxonomic status using a polyphasic taxonomic approach. Phylogenetic analysis of 16S rRNA genes and whole-genome comparisons indicated that strains CPCC 205333 T, CPCC 205515 T, and CPCC 206044 T represent three distinct novel species. The overall genome relatedness indices of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between these studied strains and their related type strains of the genus Gordonia were all below the established thresholds for species delineation, confirming the classification of these three as novel species, for which we propose the names Gordonia altitudinis sp. nov., Gordonia ligustrum sp. nov., and Gordonia pistacia sp. nov., respectively. Functional annotation revealed their ecological versatility, with Gordonia spp. contributing significantly to soil microbiome functionality through plant growth-promoting traits (e.g., nitrogen fixation, siderophore production) and biosynthetic gene clusters (BGCs), while also harboring virulence factors. Pan-genomic analysis of 225 Gordonia strains delineated an open gene pool (α = 0.82; 22

An innovative strategy has been developed to utilize distillers grains (DGS) as a substrate for solid-state fermentation (SSF) to synthesize echinocandin B (ECB). In this study, the optimal fermentation parameters for Aspergillus pachycristatus were determined as follows: an inoculation density of 1 × 106 spores/gram dry substrate (gds), pH maintained at 6.0, temperature controlled at 30℃, moisture kept at 60% (v/w), and cultivation duration set to 6 days. Engineered Trichoderma reesei is employed to pretreat DGS and enhance the release of nutrient substances for subsequent utilization by A. pachycristatus. Furthermore, incorporating an amino acid cocktail augments metabolic flux towards ECB synthesis. Ultimately, these efforts result in a production yield of 2017.6 μg/gds for ECB. This study not only provides a solution for the high-value utilization of DGS but also presents an efficient, cost-effective, and environmentally friendly synthetic process within the circular economy framework for industrial production of ECB.

The Arctic and Antarctic regions are characterized by low temperatures, high solar irradiation, and successive freezing and thawing cycles. To date, 57 yeast species belonging to 24 genera have been identified as novel taxa initially isolated from Arctic and Antarctic environments. This study comprehensively explored yeast diversity in diverse habitats, including soil, freshwater, seawater, lichens, mosses, vascular plants, dung, feathers, algae, and mushrooms, in the Ny-Ålesund (Arctic) and Fildes regions (Antarctica). Over the past decade, a total of 406 samples were collected, resulting in the isolation of 2215 yeast strains. Molecular analysis revealed 2150 yeast strains across 80 known species in 36 genera. Remarkably, 65 yeast strains - 33 from Antarctica and 32 from the Arctic - were classified as novel taxa. Based on phylogenetic and phenotypic analyses, we described 18 new basidiomycetous yeast species across two classes: Tremellomycetes within Agaricomycotina and Microbotryomycetes within Pucciniomycotina. Furthermore, we proposed the establishment of one new family, Pricozymaceae, and five new genera, including Pricozyma, Xiangyanghongia, Chioneozyma, Skadia, and Xuelongia. In summary, this study revealed a rich diversity of yeast species in the Arctic and Antarctica, identifying 98 species across 40 genera, 22 families, 12 orders, four classes, and two phyla, many of which were previously unknown. Novel species described include: Chioneozyma fusiformis, Chioneozyma ovata, Dioszegia frigidiaquatica, Dioszegia dongchenii, Fellozyma antarctica, Genolevuria ovata, Glaciozyma ellipsoidea, Glaciozyma elongata, Phaeotremella nansenii, Phaeotremella polaris, Pseudotremella lichenophila, Piskurozyma viscida, Pricozyma crymophila, Skadia corniformis, Skadia rubropurpurea, Xiangyanghongia terricola, Xuelongia filamentosa, and Yunzhangia cylindrica. Citation: Feng JJ, He CY, Su J, Zhu YW, Mi ZC, Yu LY, Zhang T (2025). Diversity and phylogeny of yeasts in various habitats of the Arctic and Antarctic regions, with descriptions of one new family, five new genera and eighteen new species. Persoonia 55: 93-140. doi: 10.3114/persoonia.2025.55.03.

Five Gram-stain-positive, aerobic, non-motile actinobacterial strains designated as CPCC 205763T, CPCC 203386T, CPCC 205716T, CPCC 203406T, and CPCC 203407 were obtained from different ecosystems associated with four kinds of Chinese traditional medicinal plants. The 16S rRNA gene sequences of these five strains showed closely related to members of the genus Herbiconiux of the family Microbacteriaceae, with the highest similarities of 97.4–99.7% to the four validly named species of Herbiconiux. In the phylogenetic trees based on 16S rRNA gene sequences and the core genome, these isolates clustered into the clade of the genus Herbiconiux within the lineage of the family Microbacteriaceae. The overall genome relatedness indexes (values of ANI and dDDH) and the phenotypic properties (morphological, physiological and chemotaxonomic characteristics) of these isolates, readily supported to affiliate them to the genus Herbiconiux, representing four novel species, with the isolates CPCC 203406T and CPCC 203407 being classified in the same species. For which the names Herbiconiux aconitum sp. nov. (type strain CPCC 205763T = I19A-01430T = CGMCC 1.60067T), Herbiconiux daphne sp. nov. (type strain CPCC 203386T = I10A-01569T = DSM 24546T = KCTC 19839T), Herbiconiux gentiana sp. nov. (type strain CPCC 205716T = I21A-01427T = CGMCC 1.60064T), and Herbiconiux oxytropis sp. nov. (type strain CPCC 203406T = I10A-02268T = DSM 24549T = KCTC 19840T) were proposed, respectively. In the genomes of these five strains, the putative encoding genes for amidase, endoglucanase, phosphatase, and superoxidative dismutase were retrieved, which were classified as biosynthetic genes/gene-clusters regarding plant growth-promotion (PGP) functions. The positive results from IAA-producing, cellulose-degrading and anti-oxidation experiments further approved their potential PGP bio-functions. Pangenome analysis of the genus Herbiconiux supported the polyphasic taxonomy results and confirmed their bio-function potential.

Three chromomycin derivatives, chromomycins A3 (1, CA3), A5 (2, CA5), and monodeacetylchromomycin A3 (3, MDA-CA3), were identified from the soil-derived Streptomyces sp. CGMCC 26516. A reinvestigation of the structure of CA5 is reported, of which the absolute configuration was unambiguously determined for the first time to be identical with that of CA3 based on nuclear magnetic resonance (NMR) data analysis as well as NMR and electronic circular dichroism calculations. Compounds 1–3 showed potent cytotoxicity against the non-small-cell lung cancer (NSCLC) cells (A549, H460, H157-c-FLIP, and H157-LacZ) and down-regulated the protein expression of c-FLIP in A549 cells. The IC50 values of chromomycins in H157-c-FLIP were higher than that in H157-LacZ. Furthermore, si-c-FLIP promoted anti-proliferation effect of chromomycins in NSCLC cells. In nude mice xenograft model, 1 and 2 both showed more potent inhibition on the growth of H157-lacZ xenografts than that of H157-c-FLIP xenografts. These results verify that c-FLIP mediates the anticancer effects of chromomycins in NSCLC.

A Gram-negative, ellipsoidal to short-rod-shaped, motile bacterium was isolated from Beijing's urban air. The isolate exhibited the closest kinship with Noviherbaspirillum aerium 122213-3T, exhibiting 98.4 % 16S rRNA gene sequence similarity. Phylogenetic analyses based on 16S rRNA gene sequences and genomes showed that it clustered closely with N. aerium 122213-3T, thus forming a distinct phylogenetic lineage within the genus Noviherbaspirillum. The average nucleotide identity and digital DNA–DNA hybridization values between strain I16B-00201T and N. aerium 122213-3T were 84.6 and 29.4 %, respectively. The respiratory ubiquinone was ubiquinone 8. The major fatty acids (>10 %) were summed feature 3 (C16:1ω6c/C16:1ω7c, 43.3 %), summed feature 8 (C18:1ω7c/C18:1ω6c, 15.9 %) and C12:0 (11.0 %). The polyamine profile showed putrescine as the predominant compound. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, unknown lipids and unknown phosphatidylaminolipids. The phenotypic, phylogenetic and chemotaxonomic results consistently supported that strain I16B-00201T represented a novel species of the genus Noviherbaspirillum, for which the name Noviherbaspirillum album sp. nov. is proposed, with I16B-00201T (=CPCC 100848T=KCTC 52095T) designated as the type strain. Its DNA G+C content is 59.4 mol%. Pan-genome analysis indicated that some Noviherbaspirillum species possess diverse nitrogen and aromatic compound metabolism pathways, suggesting their potential value in pollutant treatment.

Species of Antarctomyces and Thelebolus (Thelebolaceae), primarily found in Antarctic environments, exhibit psychrophilic adaptations, yet their mitochondrial genomes have not been extensively studied. Furthermore, few studies have compared the mitochondrial genomes of psychrophilic, psychrotrophic, and mesophilic fungi. After successful sequencing and assembly, this study annotated the mitochondrial genomes of Antarctomyces psychrotrophicus CPCC 401038 and Thelebolus microsporus CPCC 401041. We also performed a comparative analysis with the previously characterized mitochondrial genomes of psychrotrophic and mesophilic fungi. The analysis revealed that nad4L was the most conserved gene across the mitochondrial genomes, characterized by its synonymous and non-synonymous substitution rates (Ks and Ka), genetic distance, and GC content and skew within the protein-coding genes (PCGs). Additionally, the mitochondrial genomes of psychrophilic and psychrotrophic fungi showed a higher proportion of protein-coding regions and a lower GC content compared to those of mesophilic fungi, underscoring the genetic basis of cold adaptation. Phylogenetic analyses based on these mitochondrial genes also confirmed the phylogenetic relationships of Thelebolaceae in the class Leotiomycetes. These findings advance our understanding of the phylogenetic relationships and evolutionary dynamics within the family Thelebolaceae, highlighting how different environmental temperatures influence fungal mitochondrial genomic structure and adaptation.

Solirubrobacter spp. were abundant in soil samples collected from deserts and other areas with high UV radiation. In addition, a novel Solirubrobacter species, with strain CPCC 204708T as the type, was isolated and identified from sandy soil sample collected from the Badain Jaran Desert of the Inner Mongolia autonomous region. Strain CPCC 204708T was Gram-stain positive, rod-shaped, non-motile, non-spore-forming, and grew optimally at 28–30°C, pH 7.0–8.0, and in the absence of NaCl. Analysis of the 16S rRNA gene sequence of strain CPCC 204708T showed its identity within the genus Solirubrobacter, with highest nucleotide similarities (97.4–98.2%) to other named Solirubrobacter species. Phylogenetic and genomic analyses indicated that the strain was most closely related to Solirubrobacter phytolaccae KCTC 29190T, while represented a distinct species, as confirmed from physiological properties and comparison. The name Solirubrobacter deserti sp. nov. was consequently proposed, with CPCC 204708T (= DSM 105495T = NBRC 112942T) as the type strain. Genomic analyses of the Solirubrobacter spp. also suggested that Solirubrobacter sp. URHD0082 represents a novel species, for which the name Candidatus “Solirubrobacter pratensis” sp. nov. was proposed. Genomic analysis of CPCC 204708T revealed the presence of genes related to its adaptation to the harsh environments of deserts and may also harbor genes functional in plant-microbe interactions. Pan-genomic analysis of available Solirubrobacter spp. confirmed the presence of many of the above genes as core components of Solirubrobacter genomes and suggests they may possess beneficial potential for their associate plant and may be important resources for bioactive compounds.

[This corrects the article DOI: 10.3389/fmicb.2022.1034816.].

On the basis of the one strain-many compounds (OSMAC) strategy, two new cyclic thiopeptides, geninthiocins E and F, together with four known geninthiocin derivatives, geninthiocins A, B, C, and val-geninthiocin were isolated from Streptomyces sp. CPCC 200267. Their structures and absolute configurations were elucidated by extensive spectroscopic analyses and Marfey’s method. Geninthiocin E (1), val-geninthiocin (3), geninthiocin A (4), and geninthiocin B (5) exhibited significant anti-influenza A virus activities with the IC50 values of 28.7, 15.3, 7.3, and 18.3 μM, respectively. Compounds 3 and 4 showed moderate antibacterial activities against Staphylococcus aureus.

Mycobacterium species exhibit high bioremediation potential for the degradation of polycyclic aromatic hydrocarbons (PAHs) that are significant environmental pollutants. In this study, three Gram-positive, rapidly growing strains (YC-RL4(T), MB418(T), and HX176(T)) were isolated from petroleum-contaminated soils and were classified as Mycobacterium within the family Mycobacteriaceae. Genomic average nucleotide identity (ANI; <95%) and digital DNA-DNA hybridization (dDDH; <70%) values relative to other Mycobacterium spp. indicated that the strains represented novel species. The morphological, physiological, and chemotaxonomic characteristics of the isolates also supported their affiliation with Mycobacterium and their delineation as novel species. The strains were identified as Mycobacterium adipatum sp. nov. (type strain YC-RL4(T) = CPCC 205684(T) = CGMCC 1.62027(T)), Mycobacterium deserti sp. nov. (type strain MB418(T) = CPCC 205710(T) = KCTC 49782(T)), and Mycobacterium hippophais sp. nov. (type strain HX176(T) = CPCC 205372(T) = KCTC 49413(T)). Genes encoding enzymes involved in PAH degradation and metal resistance were present in the genomes of all three strains. Specifically, genes encoding alpha subunits of aromatic ring-hydroxylating dioxygenases were encoded by the genomes. The genes were also identified as core genes in a pangenomic analysis of the three strains along with 70 phylogenetically related mycobacterial strains that were previously classified as Mycolicibacterium. Notably, strain YC-RL4(T) could not only utilize phthalates as their sole carbon source for growth, but also convert di-(2-ethylhexyl) phthalate into phthalic acid. These results indicated that strains YC-RL4(T), MB418(T), and HX176(T) were important resources with significant bioremediation potential in soils contaminated by PAHs and heavy metals.

Postgenomic analysis manifested that filamentous fungi contain numerous natural product biosynthetic gene clusters in their genome, yet most clusters remain cryptic or down-regulated. Herein, we report the successful manipulation of strain Aspergillus sp. CPCC 400735 that enables its genetic engineering via targeted overexpression of pathway-specific transcriptional regulator AspE. The down-regulated metabolic pathway encoded by the biosynthetic gene cluster asp was successfully up-activated. Analyses of mutant Ai-OE::aspE extracts led to isolation and characterization of 13 asperphenalenone derivatives, of which 11 of them are new compounds. All of the asperphenalenones exhibited conspicuous anti-influenza A virus effects with IC50 values of 0.45-2.22 μM. Additionally, their identification provided insight into biosynthesis of asperphenalenones and might benefit studies of downstream combinatorial biosynthesis. Our study further demonstrates the effective application of targeted overexpressing pathway-specific activator and novel metabolite discovery in microorganisms. These will accelerate the exploitation of the untapped resources and biosynthetic capability in filamentous fungi.

Particulate matter (PM) has been a threat to the environment and public health in the metropolises of developing industrial countries such as Beijing. The microorganisms associated with PM have an impact on human health if they are exposed to the respiratory tract persistently. There are few reports on the microbial resources collected from PM and their antimicrobial activities. In this study, we greatly expanded the diversity of available commensal organisms by collecting 1,258 bacterial and 456 fungal isolates from 63 PM samples. A total of 77 bacterial genera and 35 fungal genera were included in our pure cultures, with Bacillus as the most prevalent cultured bacterial genus, Aspergillus, and Penicillium as the most prevalent fungal ones. During heavy-haze days, the numbers of colony-forming units (CFUs) and isolates of bacteria and fungi were decreased. Bacillus, Paenibacillus, and Chaetomium were found to be enriched during haze days, while Kocuria, Microbacterium, and Penicillium were found to be enriched during non-haze days. Antimicrobial activity against common pathogens have been found in 40 bacterial representatives and 1 fungal representative. The collection of airborne strains will provide a basis to greatly increase our understanding of the relationship between bacteria and fungi associated with PM and human health.

Protein profiling based on matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has proved to be a powerful tool for yeast identification. However, it is rarely used in the identification of yeast isolates from polar regions, which may be due to the limited data available for the differentiation of polar yeast species. The present study constructed a supplementary database of MALDI-TOF MS, including 33 yeast species from the Arctic and Antarctica. These yeast species were used to assess the accuracy and practicality of MALDI-TOF MS-based identification compared to the ribosomal DNA [internal transcribed spacer (ITS) and large subunit (LSU) gene regions] sequencing identification. Their dendrogram based on main spectra profiles (MSPs) in the supplementary database was somewhat consistent with their phylogenetic tree. The accuracy of MALDI-TOF MS identification was also compared by the ethanol-formic acid extraction method and the on-plate extraction method. In addition, peptide markers of some yeast species (e.g., Glaciozyma , Phenoliferia , Mrakia , and Vishniacozyma ) were identified. It is concluded that the MALDI-TOF MS method can differentiate some closely related yeast species from polar regions, thus is suitable for the identification of polar yeasts.

A Gram- stain-negative, rod-shaped, microcystin-degrading bacterium, designated as CPCC 100929T, was isolated from a fresh water reservoir in Sichuan Province, PR China. This isolate grew well at 4-37 degrees C and pH 6.0-8.0, with optimal growth at 28-32 degrees C and pH 7.0, respectively. The major cellular fatty acids were C18:1 w7c/C18:1 w6c, C16:0, C18:1 w7c 11-methyl and C19:0 cyclo w8c. The predominant respiratory quinone was Q-10. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylcholine were detected in the polar lipids extraction. The 16S rRNA gene sequence of strain CPCC 100929T was closely related to those of members of the genus Shinella, with the highest similarity of 98.6 % to Shinella zoogloeoides DSM 287T and 97.4-98.4 % with other identified Shinella members. In the phylogenetic trees based on 16S rRNA gene sequences and the core -genes analysis, strain CPCC 100929T was included within the clade of the genus Shinella. The values of average nucleotide identity (81.4-86.7 %) and digital DNA-DNA hybridization (25.4-44.6 %) between strain CPCC 100929T and other Shinella species were all below the thresholds for bacterial species delineation, respectively. The genomic DNA G+C content of strain CPCC 100929T was 63.6 %. The genomic sequence analysis indicated that this species contained genes encoding peroxidase, bla carbapenemase and the key enzyme for microcystin bio degradation, as well as rich carbohydrate-active enzyme coding genes, which might endow the micro-organism with properties to adapt to diverse environ-ments. Based on its phenotypic and genetic properties, we propose that strain CPCC 100929T (=T1A350T=KCTC 72957T) is the type strain of a novel species with the name Shinella lacus sp. nov.

Two Gram-staining negative strains (CPCC 101082T and CPCC 101083T) were isolated from biological sandy soil crusts samples collected from Badain Jaran desert, China. Both isolates were heterotrophic phototroph, could produce indole-3-acetic acid. The 16S rRNA gene sequences of these two strains were closely related to the members of the family Geminicoccaceae, showing high similarities with Geminicoccus roseus DSM 18922T (96.9%) and Arboricoccus pini B29T1T (90.1%), respectively. In phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 101082T and CPCC 101083T formed a robust distinct clade with Geminicoccus roseus DSM 18922T within the family Geminicoccaceae, which indicated that these two isolates could be classified into the genus Geminicoccus. The growth of strain CPCC 101082T occurred at 15–42°C and pH 4.0–10.0 (optima at 28–37°C and pH 6.0–8.0). The growth of strain CPCC 101083T occurred at 4–45°C and pH 4.0–10.0 (optima at 25–30°C and pH 6.0–8.0). The major cellular fatty acids of CPCC 101082T and CPCC 101083T contained C18:1ω7c/C18:1ω6c, cyclo-C19:0ω8c, and C16:0. Q-10 was detected as the sole respiratory quinone. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified phospholipid and an unidentified aminolipid were tested in the polar lipids profile. The genomes of the two isolates were characterized as about 5.9 Mbp in size with the G + C content of nearly 68%. The IAA-producing encoding genes were predicated in both genomes. The values of average nucleotide identity were 80.6, 81.2 and 92.4% based on a pairwise comparison of the genomes of strains CPCC 101082T and CPCC 101083T and Geminicoccus roseus DSM 18922T, respectively. On the basis of the genotypic, chemotaxonomic and phenotypic characteristics, the strains CPCC 101082T (=NBRC 113513T = KCTC 62853T) and CPCC 101083T (=NBRC 113514T = KCTC 62854T) are proposed to represent two novel species of the genus Geminicoccus with the names Geminicoccus flavidas sp. nov. and Geminicoccus harenae sp. nov.

Visual storytelling is a creative and challenging task, aiming to automatically generate a story-like description for a sequence of images. The descriptions generated by previous visual storytelling approaches lack coherence because they use word-level sequence generation methods and do not adequately consider sentence-level dependencies. To tackle this problem, we propose a novel hierarchical visual storytelling framework which separately models sentence-level and word-level semantics. We use the transformer-based BERT to obtain embeddings for sentences and words. We then employ a hierarchical LSTM network: the bottom LSTM receives as input the sentence vector representation from BERT, to learn the dependencies between the sentences corresponding to images, and the top LSTM is responsible for generating the corresponding word vector representations, taking input from the bottom LSTM. Experimental results demonstrate that our model outperforms most closely related baselines under automatic evaluation metrics BLEU and CIDEr, and also show the effectiveness of our method with human evaluation.

Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.) G. Don, was taxonomically characterized based on a polyphasic investigation. It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species. The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species. The menaquinone was MK-8(H4). The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs). The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0. The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp. The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp. nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain. The DNA G+C composition is 72.3 mol%. The availability of new data allows for an emended description of the genus Ornithinimicrobium.