痛风是一种嘌呤代谢紊乱导致的炎症性疾病,表现为尿酸升高、阵发性关节肿痛、痛风结石形成和关节畸形等,严重影响患者的工作及生活质量.随着生活方式的改变,痛风的患病率不断升高.嘌呤代谢障碍和炎症通路异常在痛风的发病机制中起主要作用.研究表明痛风会引起肠道菌群的改变,肠道菌群不仅能够影响嘌呤代谢或调节炎症,还能够影响药物对痛风的治疗效果.因此本综述从肠道菌群调节嘌呤代谢、炎症反应及药物作用等方面,分析肠道菌群在痛风发病及治疗中的作用及功能,有助于阐明痛风与肠道菌群的相关性,为更好地诊治痛风提供新思路.

Objective To study the diagnostic value of combined detection of serum CEA,CA199,CA125,AFP and fecal occult blood(FOB) in Dukes B stage colon carcinoma.Methods The above indicators in 56 patients with colon carcinoma (colon carcinoma group),35 patients with colonic polyp benign disease and 41 healthy individuals undergoing physical examination were measured.Then the sensitivity,and specificity of single index detection and their combined detection were analyzed.Results The levels and positive rates of CEA,CA199,CA125 and FOB in the colon cancer group were significantly higher than those in the colonic polyp group and healthy control group (P＜0.05),but the AFP level and positive rate had no statistical difference compared with the colonic polyp group.Serum CEA,CA125,CA199 and FOB were selected into the Logistic regression equation.The corresponding areas under the curve (AUC) were 0.745,0.886,0.792 and 0.864 respectively;the positive detection rates were 48.2 ％,35.7 ％,39.3 ％ and 78.6 ％ respectively.The combined detection could increased the specificity,sensitivity and AUC to 87.5 ％,92.1％ and 0.957,which were more superior to that in the single index detection.Conclusion The combined detection of serum CEA,CA199,CA 125 and FOB can provide the powerful basis for early diagnosing colonic cancer.

The aim is to evaluated the effect and mechanism of sulphasalazine (SSZ) in murine model of collage‐induced arthritis (CIA ) on modulating the balance of Th17/Treg cells by establishing the CIA model .After 21 days ,DBA1 mice were divided into CIA and CIA + SSZ group .The severity of arthritis joint was assessed by using arthritis score .The numbers of Th17 cells and CD4 + CD25 + Foxp3 + Tregs were determined by flow cytometry .In comparison with CIA group ,CIA + SSZ group reduced the disease severity obviously (p < 0.05) .Sulphasalazine reduced Th17 cells (p < 0.05) .In addition ,SSZ has effect on Treg cells’ number .These results indicate that SSZ effect on the balance of Th 17/Treg through sup‐pressing Th17 cells ,and stimulating Treg cells .Sulphasalazine may act as a potential immunomodulator for the treatment of rheumatoid arthritis (RA) .

Objective :To optimize the formula and preparation technique of doxorubicin liposome, and to e-valuate its cytotoxicity on human ovarian cancer HO8910 cells in vitro with rhythmical low-dosage admin-istration. Methods:Doxorubicin liposome was prepared by the (NH4 )2SO4-gradient method. The opti-mum formation was selected by means of the orthogonal design of experiment .The in vitro drug release behavior of liposome was evaluated by the dialysis method. MTT assay was applied to investigate the cyto-toxicity of doxorubicin liposome and free doxorubicin with common administration after 24h and rhythmi-cal low-dosage administration after 144h on HO8910 cells .Results :The optimum formula was as follows :the ratio of lecithin to cholesterol was 3∶1, (NH4)2SO4 concentration was 155 mmol/L ,drug∶lecithin was 1∶10 ,temperature was 60 ℃ .The average entrapment efficiency for doxorubicin was 92.86% .The release of liosome was relatively slow. The cytotoxicity test showed that IC50 of free doxorubicin and doxo-rubicin liposomes were 0.62 μg/mL and 0.46 μg/mL respectively at 24h and 0.06 μg/mL and 0.01 μg/mL at 144h .Conclusion :The selected formulation and preparation technique of doxorubicin liposome is reasonable in prescription ,practicable in techniques and high in encapsulation efficiency. MTT indicated that the inhibition rates of free doxorubicin and doxorubicin liposomes with rhythmical low-dosage admin-istration were much higher than those with MTD chemotherapy administration, and rhythmical low-dos-age administration had much higher inhibition rate for HO 8910 cells than free drug .

Objective:To explore the role of growth factor receptor bound 7(GRB7) during embryo implantation through detecting its expression pattern in the mouse endometrium.Methods:Expression of GRB7 mRNA and protein in the non-pregnant and pregnant mouse endometrium from d1 to d7 was examined by qRT-PCR,immunohistochemistry and Western blot.GRB7 antisense oligo-nucleotide was injected at 8 pm on pregnancy d3 and embryo implantation number on pregnancy d7 was examined.Results:GRB7 mRNA expressed in uterine endometrium of the non-pregnant and pregnant mouse from d1 to d7 and reached the highest level on pregnant d5 based on the results of qRT-PCR.Immunohistochemistry and Western blot showed that the expression pattern of GRB7 in mouse endometrium was the same with that detected by qRT-PCR.Moreover,from pregnant d1 to d4,GRB7 was mainly expressed in epithelium and glandular cells and was lowly expressed in stromal cells;on d5 and d6,GRB7 was more intense in epithelium and glandular cells and was obviously expressed in stromal cells,especially on d5.On d6,GRB7 was expressed strongly in primary decidual zones at implantation sites and stromal cells at inter-implantation sites,respectively.After horn injecting GRB7 antisense oligonucleotides,the number of embryo implantation was obviously decreased when compared with that in control group.Conclusions:Differential expression of GRB7 in pregnant mouse endometrium suggests that GRB7 may involve in the process of embryo implantation.