A new flavonol glycoside that was called rosanoside was isolated from the aerial part of the plant Oxytropis rosea. Its structure was established by analyzing data from 1H and 13C NMR spectra and DEPT, HSQC, and HMBC experiments. Rosanoside had the structure rhamnocitrin-3-O-(6′′-crotonoyl-β-D-glucopyranoside).

Pulicaria salviifolia and P. gnaphalodes belong to one of the richest terpenoid plants of the genus Pulicaria (family Asteraceae). The genus includes 63 species, 16 species grow in Uzbekistan. In folk medicine, plants are actively used to treat various diseases, and have long been used as a natural remedy for mosquitoes, flies and fleas. Secondary plant metabolites are of great practical interest as a natural raw material for the creation of biopesticides. The aim of this work was to study the chemical composition of the volatile components of gasoline fractions of Pulicaria salviifolia and P. gnaphalodes plants and to study their insecticidal activity against the number of insect pests. For the first time, the composition of volatiles in the aerial parts of Pulicaria salviifolia and P. gnaphalodes was studied by GC/MS. It was revealed that the predominant components of P. salviifolia are β-terpineol (10.44%), isospatulenol (8.00%), iceol (6.79%), sabinene hydrate (5.72%), l-caryophyllene (4.24%), terpinene-4-ol (4.07%), d-cadinene (3.65%), α-amorphous (2.30%). Calaminene (12.44%), 2-phenylpyrazol-3-amine (8.61%), 1,3,5-cycloheptatriene (7.71%), 1,8-cineole (6.06%), 2-heptene dominate among the volatile substances of P. gnaphalodes-1-ol, 2-methyl-6-(4-methylphenyl)-, (E)- (6.04%), 1-bromohomoadamantane (5.73%), 7-acetyl-2-hydroxy-2-methyl-5-isopropylbicyclo[4.3.0]nonane (4.83%). Gasoline fractions of Pulicaria salviifolia and P. gnaphalodes were tested for insecticidal activity against apple aphid (Aphis pomi), peach aphid (Myzus persicae Sulzer) and cowpea weevils (Callosobruchus maculatus). An insecticidal activity was studied in laboratory conditions. A comparative study of the biological activity of the fractions showed that the biological efficiency of the P. gnaphalodes extract fraction after 24 hours at 0.5% concentration was higher: in relation to Aphis pomi 96.6%, Myzus persicae Sulzer – 76.0% and Callosobruchus maculatus – 90.0%, for the fraction of Pulicaria salviifolia extract in a 0.5% dose, these indicators were 60.2%, 72.0% and 86.6%, respectively.

Rumex tianschanicus is an important medicinal plant in Uzbekistan.The activity of the underground parts of the leaves of the plant was studied using traditional medicine.Infusion and alcoholic extract are used for treatment.Fruits are used to treat dyspepsia in children.The object of research is the Rumex tianschanicus plant growing in Khojaariq village, Sariosiya district, Surkhandarya region.Above-ground part of dried plant of Rumex tianschanicus was extracted with 70% ethanol and obtained four fraction: Extraction benzene, chloroform.ethyl acetate and n-butanol.All fractions sdudied antibacterial activity.n-Butanol fraction shoved higly antibacterial activity.The n-butanol fraction subjected to column chromatography with silica gel, by the sistem khloroform-methanol 50:1.In thechloroform methanol 25:1 system isolated one compound glucoze kharakteric and the crystal was grown.Its structure was determined using x-ray structural analysis (RSA).This is a known substance, but it was obtained for the first time from Rumex tianschanicus.

. The aim of this research is to study the biological activity of phenolic compounds of Rumex pamiricus plant in Uzbekistan. Two known anthraquinone derivatives, chrysophanol and emodin has been isolated from the chloroform fraction extract of Rumex pamiricus roots. Chrysophanol and emodin were tested in vitro for antioxidant and antihypoxic activity.

Three known anthraquinone derivatives were extracted from Rumex pamiricus roots chloroform fraction extract, while two known compounds were obtained from Rumex conglomeratus roots ethyl acetate fraction extract. Antimicrobial activity of essential oils from Rumex confertus and Rumex pamiricus aerial parts, gasoline, chloroform, ethyl acetate, and butanol extracts of Rumex pamiricus aerial parts, and alcohol extract of Rumex pamiricus roots was investigated in vitro.

Из надземной части полыни арги (Artemisia argyi H. Lév. & Vaniot) выделены и идентифицированы флавоноиды с использованием современных физико-химических методов (ИК-, УФ-, ЯМР-спектроскопии и гель-хроматографии), относящиеся к группе флавонов: гиспидулин (5,7,4-тригидрокси-6-метоксифлавон), трицин (5,7,4’-тригидрокси-3’,5’-диметоксифлавон), ладанеин (5,6-дигидрокси-4’,7-диметоксифлавон), эупатилин (5,7-дигидрокси-3’,4’,6-триметоксифлавон), кемпферол (3,4’,5,7-тетрагидроксифлавон), кверцетин (3,3’,4’,5,7-пентагидроксифлавон), лютеолин (3’,4’,5,7-тетрагидроксифлавон) ранее описанный для данного вида. Флавоноиды трицин и ладанеин были выделены из этого растения и идентифицированы впервые.

Flavonoids of the aerial part of Chinese mugwort (Artemisia argyi H. Lev. & Vaniot) were extracted and identified using modern physicochemical methods (IR, UV, and NMR spectroscopy and gel chromatography). They belonged to the flavone group and included hispidulin (5,7,4′-trihydroxy-6-methoxyflavone), tricin (5,7,4′-trihydroxy-3′,5′-dimethoxyflavone), ladanein (5,6-dihydroxy-4′,7-dimethoxyflavone), eupatilin (5,7-dihydroxy-3′,4′,6-trimethoxyflavone), kaempferol (3,4′,5,7-tetrahydroxyflavone), quercetin (3,3′,4′,5,7-pentahydroxyflavone), and luteolin (3′,4′,5,7-tetrahydroxyflavone), which were described earlier for this genus. The flavonoids tricin and ladanein were isolated from this plant for the first time.

In this study, we have determined the antimicrobial, antioxidant activity, the content of phenols and flavonoid in extracts and fractions of plants belonging to the genus Rumex (Polygonaceae), widely distributed in the flora of Uzbekistan. To determine the antimicrobial activity of extracts and fractions, test strains of E. coli, B. subtilis, P. aureginosa, S. aureus, C. albicanc were used. To determine the antioxidant activity, standard solution of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used. The best results for the total content of phenols and flavonoids in the extracts were as follows: ethanol extract of R. tianschanicus (TPC) 41.23 +/- 4.10 mg GAE/g, (TFC) 58.41 +/- 2.18 mg CE/g. With regard to antimicrobial activity, the following results were obtained. The R. confertus EtOH fraction showed higher antimicrobial properties against E. coli (17 +/- 0.12), B. subtilis (14 +/- 0.17), P. aureginosa (13 +/- 0.10), C. albicans (17 +/- 0.10) compared to other samples. The Rumex pomiricus CHCl3 fraction showed high antimicrobial properties against E. coli (15 +/- 1.21), B. subtilis (13 +/- 1.01), P. aureginosa (20 +/- 0.21), C. albicans (25 +/- 0.13). The results of the minimum inhibitory concentration were as follows: extracts of R. pomiricus MIC (6.25 +/- 0.02 mu g/ml); MIC of R. pomiricus CHC l3 fraction (6.25 +/- 0.107 mu g/ml). In terms of antioxidant activity (DPPH), IC50 values (p<0.01) for ascorbic acid 24.53 mu g/ml, R. confertus EtOH fraction 134.9 mu g/ ml significant changes were noted. R. syriacus leaf extract 70% showed IC50 values (p<0.01) of 48.54 mu g/ml.

The aim of the present study to clarify the phytochemical constituents of Rumex plants, extraction, separation and structural identification of chemical components, provide theoretical support and scientific basis for its traditional use, further expand the application scope of traditional medicinal plant resources in Uzbekistan, and develop its potential value. Three known anthraquinone derivatives has been isolated from the chloroform fraction extract of Rumex pamiricus roots and two known compounds were separated from the ethyl acetate fraction extract of Rumex conglomeratus roots. Their structures were elucidated by extensive spectroscopic evidence and chemical methods. Qualitative analyses of major phenolics by TLC analysis were also evaluated. The ester oil from the aerial parts of Rumex confertus and Rumex pamiricus, the gasoline, chloroform, ethyl acetate and butanol extracts of Rumex pamiricus aerial part and alcohol extract of Rumex pamiricus roots were tested in vitro for antimicrobial activity. This study aimed to determine the in vitro antimicrobial activity of the medicinal plants traditionally used in Uzbekistan against the microbial strains associated with infectious diseases.

. In this work, the amount of tannins in the root and leaves of Rumex confertus Willd and Rumex pamiricus Rech. f. plants were determined by using standard method. As a result, it was found that the amount of tannins in the root of Rumex confertus Willd is 19.1%, in the leaves – 7.36%, and in the root of Rumex pamiricus Rech. f. – 16.5%, and in the leaves – 5.2%.

Из надземной части полыни арги (Artemisia argyi H. Lév. & Vaniot) выделены и идентифицированы флавоноиды с использованием современных физико-химических методов (ИК-, УФ-, ЯМР-спектроскопии и гель-хроматографии), относящиеся к группе флавонов: гиспидулин (5,7,4-тригидрокси-6-метоксифлавон), трицин (5,7,4’-тригидрокси-3’,5’-диметоксифлавон), ладанеин (5,6-дигидрокси-4’,7-диметоксифлавон), эупатилин (5,7-дигидрокси-3’,4’,6-триметоксифлавон), кемпферол (3,4’,5,7-тетрагидроксифлавон), кверцетин (3,3’,4’,5,7-пентагидроксифлавон), лютеолин (3’,4’,5,7-тетрагидроксифлавон) ранее описанный для данного вида. Флавоноиды трицин и ладанеин были выделены из этого растения и идентифицированы впервые.

Phytochemical study on the ethanolic extract of the dried roots of Ferula samarkandica Korovin led to the isolation of nine undiscribed sesquiterpene coumarins, samarcandicins A-I, along with thirteen known sesquiterpene coumarins. Their structures were characterized by detailed spectroscopic analysis including NMR and HR-ESI-MS. Mogoltacin and nevskin exhibited high inhibitory activity against MV-4-11 cell with IC50 values of 3.94 +/- 0.06 mu M and 3.87 +/- 0.10 mu M, respectively, and nevskin and feshurin showed high inhibitory activity against mino cell with IC50 values of 1.48 +/- 0.06 mu M and 7.88 +/- 0.60 mu M, respectively.

The new flavonoid diglycoside thamiflaside was isolated from the aerial part of Thalictrum minus. The chemical structure of this glycoside was determined as apigenin 7-O-α-L-2″′-methoxyrhamnopyranosyl-(1→6)-β-D-glucopyranoside based on PMR and 13C NMR (DEPT, HSQC, HMBC) physicochemical methods.