The paper considers temperature and humidity simulation of the primary circuit pipeline rooms located at power units No. 3 and 4 of the Kola NPP upon the occurrence of equipment leakage. Simulation results were used to describe the dependence of the leakage on the temperature and relative humidity recorded by the sensors. Thus, advanced methods of automated coolant leak detection were developed; existing methods were adjusted.

The article presents the results of developing a fragment of a thermal displacement control system, writing the corresponding software for measuring the linear displacement of the monitored equipment and pipelines, and analyzing the obtained results for assessing the technical state and cyclic strength of the monitored equipment as a means of managing the resource characteristics of the controlled object. The measurement uncertainty was evaluated. Taking into account the influence of various factors, such as illumination, the degree of filling the image with the target, the distance from the video camera to the target, as well as the direction and dimensions of displacements and vibration, the volume of test checks was formed using 780 tests. In the course of the analysis, the relative error in measuring displacements taking vibrations into account was revealed to be no more than 5

The efficiency of several mouse monoclonal antibodies (mAbs) specific to the tick-borne encephalitis virus (TBEV) glycoprotein E in post-exposure prophylaxis was assessed, and mAb14D5 was shown to be the most active of all those studied. It was proven that the hybridoma cell line 14D5 produced one immunoglobulin H chain and two L chains. They were used to construct chimeric antibodies ch14D5a and ch14D5b, the affinity constants of which were 2.6 × 10(10)M(-1) and 1.0 × 10(7)M(-1), respectively, according to the SPR-based ProteOn biosensor assay. The neutralization index (IC50) of ch14D5a was 0.04 μg/ml in the focus reduction neutralization test. In in vivo experiments, ch14D5a at a dose of 10 μg/mouse resulted in a 100% survival of the mice infected with 240 LD50 of TBEV. This chimeric antibody is promising for further development of prevention and therapeutic drugs against TBEV.

Лактаптин – пептид, выделенный из молока человека и являющийся протеолитическим фрагментом k-казеина с молекулярной массой 8,6 кДа. Показано, что данный пептид вызывает апоптическую гибель клеток аденокарциномы молочной железы человека MCF-7. Для исследования противоопухолевых свойств лактаптина получен его генно-инженерный аналог, также индуцирующий апоптоз клеток MCF-7. В экспериментах in vitro и in vivo продемонстрировано, что рекомбинантный аналог лактаптина оказывает цитотоксическое действие на онкотрансформированные клетки и не токсичен по отношению к немалигнизированным клеткам. В настоящее время проводятся доклинические испытания рекомбинантного аналога лактаптина. Список ссылок 1. Некипелая В.В., Семенов Д.В., Потапенко М.О., Кулигина Е.В., Кит Ю.Я., Романова И.В., Рихтер В.А. / Докл. АН. 2008. Т. 419. С. 268-271. 2. Власов В.В., Рихтер В.А., Семенов Д.В., Некипелая В.В., Кулигина Е.В., Потапенко М.О. Пептид, обладающий апоптотической активностью по отношению к раковым клеткам человека. Патент РФ № 2317304, 2008. 3. Semenov D.V., Fomin A.S., Kuligina E.V., Koval O.A., Matveeva V.A., Babkina I.N., Tikunova N.V., Richter V.A. / Protein J. 2010. V. 29.. No. 3. P, 174 – 180. 4. Фомин А.С., Коваль О.А., Семенов Д.В., Потапенко М.О., Кулигина Е.В., Кит Ю.Я., Рихтер В.А. / Биоорг. химия. 2012. Т. 38. № 1. С. 92-98. 5. Koval O.A., Fomin A.S., Kaledin V.I., Semenov D.V., Potapenko M.O., Kuligina E.V., Nikolin V.P., Nikitenko E.V., Richter V.A. / Biochimie. 2012. V. 94. No. 12. P. 2467-2474. 6. Tijssen P. ed.by / Practice and Theory of Enzyme Immunoassays, Netherlands: Elsevier science publishers B, 1985. pp.548. 7. Егоров А. М., Осипов А. П., Дзантиев Б. Б., Гаврилова Е. М./ Теория и практика иммуноферментного анализа. – Москва: Высшая школа, 1991, 288 с. 8. ELISA technical guide and protocols, Thermo Scientific, URL: http://www.piercenet.com/files/TR0065-ELISA-guide.pdf

A new algorithm for locating a coolant leak through the analysis of temperature field values is presented. This algorithm is based on the measurements of air temperature at different points of the controlled area of the multiple forced circulation circuit of a nuclear power plant (NPP) and enables the determination of the dimension, location, and time of a coolant leak.

A new algorithm is proposed for determining the mass flow rate of coolant leaks by analysis of the absolute moisture content of air in plant buildings. The algorithm involves solving the optimal linear-filtration equations for the humidity variation in a specific building. The operation of the algorithm is demonstrated in an example.

Работа выполнена при финансовой поддержке гранта No 18 «Комплексные интеграционный проекты» Президиума СО РАН, а также ФЦНТП (гос. контракт No 02.512.11.2257).

Two chimeric antibodies (ch) 13D6 and 10C2 against the glycoprotein E of tick-borne encephalitis virus (TBEV) were constructed by fusing variable regions of murine monoclonal antibodies (Mabs) 13D6 and 10C2 to human constant regions. Monovalent analogues of these antibodies in format of single-chain antibodies (scFv or sc) were developed, as well. The ch13D6, ch10C2, sc13D6 and sc10C2 exhibited binding characteristics similar to parental Mabs. Only the ch13D6 and sc13D6 were able to neutralize TBEV infectivity in vitro. The in vitro neutralization provided by ch13D6 suggests that this antibody can be further developed into a potent prophylaxis and therapy for tick-borne encephalitis (TBE) infection.

A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in Escherichia coli cells of the scl3D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified scl3D6 was (3.0 ± 0.2) × 107 M−1 for the equilibrium state and (2.8 ± 0.3) × 107 M−1 in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC50 value for scl3D6 was 16.7 μg/ml.

Th e recombinant plasmid DNA pSC10C2 was constructed on the basis of cDNA fragments of the genes encoding variable domains of the heavy and light chains of MAb against glycoprotein E of Tick-Borne encephalitis virus (TBEV). Th is plasmid provided an expression of scFv-antibody sc10C2 against TBEV in Escherichia coli cells. Th e produced antibody was able to bind TBEV, strain 205, and recombinant protein E of TBEV. Affi nity of purifi ed sc10C2 was (1,2 ± 0,3). 107 М-1.

We present the data of 12-year survey (1989-2001) of the red vole population in southeastern West Siberia, including estimation of host relative numbers, abundance of immature taiga ticks, and percentage of animals with antigemagglutitnins against tick-borne encephalitis (TBE) virus. We discuss the role of demographic groups of voles as tick's hosts and their participation in the maintenance of TBE causative agent population. The estimation of spontaneous TBE infection rate in summer as well as in winter and early spring seasons, which have been made using a set of molecular-biological, serological and virological methods, demonstrates that a high proportion of red voles maintain non-pathogenic TBE causative agent over a long time, presumably, in the form of persistent infection.

Antigenic structure of tick-borne encephalitis virus proteins was studied by ELISA with monoclonal antibodies (MAb) to E and NS1 glycoproteins of strain Sofyin. Envelope proteins appeared to be conservative which corresponded to previously determined nucleotide sequences of E gene fragments and deduced primary structures of the corresponding E protein. Five of six studied MAb to NS1 nonstructural glycoprotein of strain Sofyin reacted with this protein of all studied strains. The only exception was MAb 17C3 which discriminates West Siberian strains from Far Eastern strain Sofyin.

HV monoclonal antibodies (MAb) were produced in order to improve the quality of HBsAg detection and their specific characteristics were compared with those of other MAbs. MAbs were characterized by asymmetric interactions with the antigen when used as first or second antibodies. The reactivity of a panel of HV and X MAb to ad and ay subtypes was studied by enzyme immunoassay. Mutual blocking (epitope mapping) of MAb helped select antibody couples for the creation of highly effective test system for the diagnosis of the major HBsAg subtypes. The sensitivity and specificity of MAbs were evaluated on reference and control panels of HBsAg sera and on serum specimens from a random sampling of 300 blood donors. The sensitivity of the most specific MAb pairs was 0.1 ng/ml for HBsAg subtype ay and 0.25 ng/ml for subtype ad. The specificity of attested MAb was 98.5% in incubation with stirring and 97% in static incubation. The optimal combinations of attested MAbs were used in the manufacture of Recomnathep B test system in the sandwich format.

Tick-borne encephalitis virus (TBEV) strains were isolated from ticks in Western Siberia for 12 years. Molecular hybridization of the 46 viral RNA with the TBEV cDNA and oligonucleotide probes revealed differences between the Siberian and Far Eastern strains. A comparison of the viral E gene fragment nucleotide sequence showed 89–98% homology between Siberian TBEV strains, whereas their similarity with strains from other populations was less than 83%. However, the viral E and NS1 glycoprotein antigenic structures appeared to be conservative because of the degenerate genetic code. This was shown by enzyme-linked immunosorbent assay with the corresponding monoclonal antibodies (MAb). The single exception was the MAb 17C3 against nonstructural glycoprotein NS1, which could distinguish Siberian from Far Eastern strains. Moreover, the neurovirulence differed between strains from the two natural populations. Lower neuroinvasiveness of the Siberian strains in comparison with Far Eastern Sofyin strain might be caused by both E and NS1 glycoprotein mutations.

Hybridomas secreting monoclonal antibodies (Mab) to tick-borne encephalitis (TBE) virus are obtained. Immunodiffusion showed that 3 Mabs to TBE protein NS3 belong to class IgM and the rest to IgG1. Mabs to TBE protein NS1 were tested in hemagglutination inhibition, complement fixation, neutralization, and protection tests. Only 1 hybridoma produced Mab specific for protein NS1 of TBE strain Sofyin, the rest reacted with the common antigenic determinants of nonstructural TBE complex.

The arrangement of envelope protein epitopes of tick-borne encephalitis viruses (TBEV) (persulcatus or eastern subtype, Sofjin strain and ricinus or western subtype, Minsk-256 strain) and Kyasanur Forest disease virus (KFDV) was investigated using competitive binding of monoclonal antibodies against the Sofjin E protein. The E protein of TBEV Sofjin strain forms three antigenic domains: E1, E2 and E3, represented by 12, 9 and 2 epitopes respectively; two additional epitopes stand alone. Domains E1 and E2 are heterogeneous. On the epitope map of the Minsk-256 strain domain E3 remains intact, domains E1 and E2 overlap and the relative arrangement of virus-neutralizing epitopes from E1 and E2 domains is changed. The epitope map of KFDV is significantly dissimilar to TBEV. The viruses can be distinguished by epitopes with identical serological reactivity. A satisfactory agreement between our epitope maps and previously published antigenic models of flavivirus envelope protein (Guirakhoo et al., 1989; Mandl et al., 1989a) was observed. The main difference of our map is that domains corresponding to domains B and C (Sofjin strain) and A, B and C (Minsk-256 strain) in Heinz's model are overlapping. The results of competition analysis depend on the nature of the antigen (virion or purified protein) and the immunoassay technique.