[目的]旨在纯化玉米ZmCCT基因缺失区段的原核表达蛋白.[方法]依据ZmCCT氨基酸序列中的CCT保守功能区,利用PCR技术获得ZmCCT基因的缺失突变体DNA片段,分别构建4个原核蛋白表达缺失区段.[结果]成功获得3个缺失区段的原核蛋白:ZmCCT1-300,ZmCCT300-540和ZmCCT1-540.[结论]该研究不仅有助于ZmCCT多克隆抗体的纯化,还将为探索ZmCCT的抗病分子机理奠定基础.

为研究不同砧木嫁接对黄瓜果实亮度和果皮细胞气孔的影响,筛选出可以改善黄瓜果皮性状、增强抗性的黄瓜砧木品种,选取了6份材料作为嫁接砧木进行比较.结果表明:双砧木(C105+京欣砧5号)嫁接后对果实产量和品质的提升效果最好,其次为C088处理,均适合以外观品质为重点的采摘观光型园区种植.如果在设施环境条件差的区域种植,如低温等其他逆境条件,建议使用黑籽南瓜作为砧木进行嫁接,但可能会降低果实的商品性,果面蜡粉层严重.

Plants possess a powerful system to defend themselves against potential threats by pathogenic fungi. For agriculturally important plants, however, current measures to combat such pathogens have proved too conservative and, thus, not sufficiently effective, and they can potentially pose environmental risks. Therefore, it is extremely necessary to identify host-resistance factors to assist in controlling plant diseases naturally through the identification of resistant germplasm, the isolation and characterization of resistance genes, and the molecular breeding of resistant cultivars. In this regard, there is need to establish an accurate, rapid, and large-scale inoculation method to breed and develop plant resistance genes. The rice blast fungal pathogen Magnaporthe grisea causes severe disease symptoms and yield losses. Recently, M. grisea has emerged as a model organism for studying the mechanisms of plant-fungal pathogen interactions. Hence, we report the development of a plant virulence test method that is specific for M. grisea. This method provides for both spray inoculation with a conidial suspension and wounding inoculation with mycelium cubes or droplets of conidial suspension. The key step of the wounding inoculation method for detached rice leaves is to make wounds on plant leaves, which avoids any interference caused by host penetration resistance. This spray/wounding protocol contributes to the rapid, accurate, and large-scale screening of the pathotypes of M. grisea isolates. This integrated and systematic plant infection method will serve as an excellent starting point for gaining a broad perspective of issues in plant pathology.

黄板诱杀是防治菇蚊蝇的常见措施.为探索平菇(Pleurotus ostreatus)菇房中,黄板悬挂位置与高度对眼蕈蚊(sciarid flies)成虫诱杀效果的影响,本试验选取日光温室型平菇菇房,在成虫盛发期,先后设置双风口和单风口两种通风模式,调查了棚室内北、中、南3个方位及其1、1.5、2、2.5 m等不同悬挂高度的黄板诱杀眼蕈蚊的数量,并分析了诱虫数量与悬挂位置的照度值之间的关系.结果显示,开双风口时,黄板悬挂在棚南侧2.0 m处时诱杀效果最佳;在只开腰风口时,最佳位置为北侧高度为1m处.回归分析表明,诱虫数量与照度值之间的线性相关不显著.