目的:建立采用1H定量核磁共振波谱法测定二对甲苯磺酸缘生替尼对照品的含量.方法:采用核磁共振波谱法.使用Bruker AscendTM 400超导核磁共振谱仪,以氘代DMSO为溶剂,脉冲宽度为10.0μs,延迟时间为1s和扫描次数32的条件下采集试样氢谱.结果:以化学位移δ分别为5.31和6.28的二对甲苯磺酸缘生替尼和顺丁烯二酸的氢质子峰作为定量峰,其峰面积比与其质量比的线性回归方程为Y=0.128 1X-0.000 4,相关系数为0.999 9,含量测定的重复性试验的RSD为0.20％(n=6).测得二对甲苯磺酸缘生替尼的含量为98.54％.结论:分析结果表明,在没有对照品的情况下,核磁共振波谱法可用于二对甲苯磺酸缘生替尼的含量测定,该方法可行,具有快速、准确、简便的优点.

目的 研究丹皮酚对LPS诱导小鼠子宫内膜炎的作用及其作用机制.方法 60只小鼠随机分为假手术组、模型组、醋酸地塞米松组(5 mg/kg)、丹皮酚高剂量组(50 mg/kg)、丹皮酚中剂量组(25 mg/kg)、丹皮酚低剂量组(12.5 mg/kg),各组连续灌胃给药5d.末次给药1h后4％水合氯醛腹腔注射麻醉(10ml/kg)后,打开腹腔,露出左侧子宫,除假手术组外,其余组用1 ml胰岛素注射器向子宫内注射(2.5 μg/μl) LPS生理盐水25μl,假手术组注射25μl生理盐水.注射24 h后脱颈椎处死小鼠,分离子宫待测.HE染色法检测子宫组织病理变化,Griess法测定NO含量,ELISA法检测炎性因子MPO、IL-1β、TNF-α含量,Western blot法检测子宫组织P65/p-P65及IκBα/p-IκBα蛋白表达变化.结果 研究结果显示丹皮酚能明显减轻LPS诱导的小鼠子宫内膜病理损伤,显著降低小鼠子宫组织MPO、NO、IL-1β、TNF-α含量,进一步研究结果显示丹皮酚可抑制小鼠子宫组织NF-κB p65、IκB蛋白的磷酸化水平.结论 丹皮酚可通过抑制NF-κB信号通路、减少炎症细胞因子的释放从而发挥抗脂多糖诱导的小鼠子宫内膜炎的作用.

原发性痛经又称为功能性痛经,是育龄女性常见的疾病之一,给女性的身心健康和工作学习带来了严重的影响,因此防治原发性痛经已成为国内外医学界亟待解决的问题.以原发性痛经病理机制为主线,将近10年来中药防治原发性痛经的机制研究进行综述,发现中医药在原发性痛经治疗方面有许多行之有效的方法,在痛经症状改善及远期疗效方面有着独特的优势,且大多数医家辨证分型论治,有内服和外用等不同方式,治疗方法灵活多样,疗效确切.

目的:研究桂枝茯苓胶囊(GZFLC)对实验性子宫内膜异位症痛经作用机制.方法:将60只SD雌性大鼠随机分为假手术组、模型组、散结镇痛胶囊组、GZFLC高、中、低剂量组(0.54、0.27、0.14 g?kg-1)组,每组10只.每天灌胃给药,连续28 d.观察各组扭体反应,测量移植物体积并进行病理检测,采用免疫组化法检测异位灶NGF、TRPV1蛋白的表达.结果:与模型组比较,散结镇痛胶囊组和GZFLC高、中、低剂量组大鼠扭体次数显著减少(P<0.01),其抑制率分别为51.1％、63.2％、60.4％、47.1％.与模型组比较,散结镇痛胶囊组和GZFLC高、中、低剂量组大鼠移植物体积显著减少(P<0.01),其抑制率分别为61.9％、66.0％、59.1％、56.6％.与模型组比较,GZFLC高、中剂量组可显著降低子宫内膜异位症痛经大鼠异位组织的病理学评分及NGF、TRPV1蛋白的表达.结论:GZFLC对实验性子宫内膜异位症痛经具有一定治疗作用,其机制与抑制NGF、TRPV1蛋白表达相关.

Objective:To establish a method for the determination of dissolution of 8 active ingredients in Guizhi Fuling capsules.Method:UPLC was employed,mobile phase of gallic acid,paeoniflorin,benzoic acid,benzoylpaeoniflorin,paeonol,cinnamic acid and cinnamaldehyde was 0.02％ trifluoroacetic acid aqueous solution-acetonitrile for gradient elution,their detection wavelengthes were 230 nm and 275 nm.The mobile phase of amygdalin was water-methanol (80∶20) and its detection wavelength was 218 nm.Result:The soluble medium was determined to be 0.1 mol· L-1 hydrochloric acid,the rotation speed was 50 r·min-1,the solubility measurement was carried out by the basket method.The linear ranges of gallic acid,paeoniflorin,benzoic acid,benzoylpaeoniflorin,paeonol,cinnamic acid,cinnamaldehyde,amygdalin were 0.097 6-24.389 9,0.097 1-24.275 4,0.052 6-5.262,0.053-5.295 6,0.108 3-27.062 9,0.050 1-5.005,0.052 4-5.236 4,0.096 6-24.139 5 mg·L-1 their average recovery rates were 102.3％ 97.3％ 99.3％ 97.9％ 97.8％ 95.8％ 97.0％ and 100.4％,respectively.The dissolution rate of samples was good,and the dissolution rate of each component in 45 min could reach ＞ 80％.Conclusion:This method is simple,accurate and reproducible,and it can be used for determination of dissolution of Guizhi Fuling capsules.

目的 建立栀子的指纹图谱并采用电喷雾离子源-四极杆-飞行时间质谱(ESI-Q-TOF MS)对其共有峰进行定性分析.方法 采用Kromasil 100-5 C18柱,以乙腈-0.1％磷酸梯度洗脱,体积流量为1mL/min,柱温为35℃,检测波长为238、327、440 nm.使用Matlab 7.1软件编程,对3波长下的CSV格式数据进行全时段融合.结果 各色谱峰分离度良好,3波长融合指纹图谱全面体现了栀子238、327、440 nm特征吸收波长的指纹信息,标定20个共有峰,并完成其中16个成分的鉴定.10批不同批次的栀子指纹图谱与对照指纹图谱之间相似度较好,相似度大于0.90以上.结论 实现了栀子融合指纹图谱的测定,同时解析了图谱中的化学组成,并制定了主成分与栀子苷相对峰面积的范围,为全面控制药材质量提供了数据支撑.

目的:对牡丹皮中Fe,Mn,Cu,Zn,A1,Pb,As,Cd等24种微量元素采用煎煮法进行提取.方法:使用0.45μm的微孔滤膜分离提取液中的悬浮态和可溶态,将可溶态通过大孔树脂(LSA-10),以去离水和甲醇洗脱,确定微量元素的无机态和有机态;模拟人体胃肠溶液,采用正辛醇-水系统分离微量元素的醇溶态和水溶态;通过ICP-MS技术(电感耦合等离子体质谱法),测定微量元素总量及各种形态.结果:方法的回收率为74.7％～118.2％,精密度RSD在1.3％～6.9％.24种微量元素的溶出率为2.0％～82.9％,可溶态在水中的比率为73.6％ ～99.5％,可溶态中80％以上微量元素以无机态和水溶态形式存在.结论:微量元素的形态分析及溶出特征为牡丹皮的临床应用提供理论依据.

目的:建立测定二对甲苯磺酸缘生替尼原料药中有关物质的方法.方法:采用高效液相色谱法.色谱柱为Waters Symmetry C18,流动相为甲醇-0.01 mol/L乙酸铵溶液(梯度洗脱),流速为1.0 mL/min,检测波长为240 nm,柱温为40℃,进样量为10 μL.结果:在该色谱条件下,主成分峰与各杂质峰分离度均良好;杂质A、B、C和二对甲苯磺酸缘生替尼检测质量浓度线性范围均为0.25～2.0 μg/mL(r≥0.999 0),杂质A、B、C的定量限分别为0.5、0.5、2.5 ng;精密度、稳定性、重复性试验的RSD＜ 1.0％;加样回收率分别为97.9％～102.6％、95.1％～107.7％、95.8％～107.5％,RSD分别为1.4％、4.2％、4.1％(n=9).结论:该方法专属性好、操作简便,可用于二对甲苯磺酸缘生替尼原料药中有关物质的测定.

Objective To establish an HPLC fingerprint of the compounds in Qibai Pingfei Granules (QPG),and to make a quantitative analysis.Methods Sample was extracted by 50％ methanol.Phenomenex Luna C18 column (250 mm × 4.6 mm,5 μm) was used with a mobile phase of methanol-0.2％ formic acid gradient elution.The flow rate was 1.0 rnL/min,the detection wavelength was 250 nm,and the column temperature was 30 ℃.The chemical component fingerprint similarity of 10 batches of QPG was calculated with Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012) published by National Pharmacopoeia Committee and the common peaks were identified by reference compounds.Results Fingerprints of 10 batches of QPG were established and the similarities to the common mode were above 0.96.Totally 25 common peaks were found.Among them,peak 1 belonged to Pheretima,peaks 2 and 3 belonged to Pheretima and Ginseng Radix et Rhizoma (GRR),peak 4 belonged to GRR,Schisandrae Chinensis Fructus (SCF),and Chuanxiong Rhizoma (CR),peak 5 belonged to GRR,Allii Macrostemonis Bulbus (AMB),CR,and Pheretima,peaks 6,8,22,23,24,and 25 belonged to SCF,peak 7 belonged to Astragali Radix (AR) and SCF,peak 9 belonged to GRR and AMB,peak 10 belonged to Descurainiae Semen Lepidii Semen (DSLS) and AMB,peaks 11,12,13,15,and 16 belonged to CR,peak 14 belonged to AR and DSLS,peaks 17,18,19,20,and 21 belonged to AR.Based on the retention time,and UV absorption spectra of reference compounds,six constituents including caffeic acid (peak 12),ferulic acid (peak 13),schizandrol A (peak 22),schisantherin A (peak 23),deoxyschizandrin (peak 24),and schisandrin (peak 25) were identified.The linear ranges of caffeic acid,ferulic acid,schizandrol A,schisantherin A,deoxyschizandrin,and schisandrin were 3.38-108.02,3.60-115.33,2.99-95.61,2.81-89.77,3.26-104.17,and 2.89-92.45 μg/mL,respectively.In 10 batches of QPG samples,the contents were as follows:caffeic acid of 0.412-0.429 mg/g,ferulic acid of 0.302-0.317 mg/g,schizandrol of A 0.182-0.195 mg/g,schisantherin A of 0.179-0.195 mg/g,deoxyschizandrin of 0.203-0.215 mg/g,and schisandrin of 0.131-0.144 mg/g,the amount of each indicator composition among different batches changed a litte,and the sample quality is stable.Conclusion The method has good precision,reproducibile,stability,separation,and can be used for the quality control of QPG.

Objective:To establish a quantitative analysis for the determination of the six components in Congrong Zonggan capsule based on multi-components with a single-marker (QAMS) method and ultra-high performance liquid chromatography (UPLC),and compare with the standard curve method,in order to evaluate the adaptation and application of QAMS method in the quality control of Congrong Zonggan capsule.Method:Chrlorogenic acid and acteoside were used as the internal reference substances.The relative correlation factors (RCF) of caffeic acid with chrlorogenic acid,as well as tubuloside A,isoacteoside and echinacoside with acteoside were calculated and established.At the same time,the durability of each RCF was investigated.The results were compared with those obtained by the standard curve method,in order to verify the accuracy and feasibility of the method.Result:Within the linear ranges,the RCF values of chlorogenic acid with caffeic acid was 0.538,and that of acteoside with tubuloside A,isoacteoside and echinacoside were 1.346,1.266 and 1.135,respectively.The durability of each RCF was good.The two methods showed no significant difference in assay results for the 10 batches of samples.The obtained RCF was credible.Conclusion:The QAMS method is feasible and credible,and could be used to determine the multiple components in Congrong Zonggan capsule.

Sanjie Zhentong capsule, a well-known traditional Chinese medicine prescription, are used for the treatment of endometriosis-related diseases. In this study, a simple, rapid and sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of ten bioactive constituents, including peimine, peiminine, peimisine, loureirin A, loureirin B, 7,4'-dihydroxyflavone, pterostilbene, ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in rat plasma after oral administration of Sanjie Zhentong capsule. The sample preparations for protein removal was accomplished using a simple methanol precipitation method. The analytes were completely separated from the endogenous compounds on an Agilent Poroshell 120 SB-C18 column (4.6mm×150mm, 2.7μm) using an isocratic elution with methanol - 0.1% formic acid aqueous (4/1, v/v) as a mobile phase. The single-run analysis time was as short as 14.0min. The inter-day and intra-day precision of the quality control samples exhibited relative standard deviations (RSD) <9.5% and the accuracy values ranged from -8.6% to 15.0%. The lower limits of quantification (LLOQ) were 10, 10, 10, 10, 10, 10, 5, 10, 10 and 20ng/mL for peimine, peiminine, peimisine, loureirin A, loureirin B, 7,4'-dihydroxyflavone, pterostilbene, ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, respectively. The analytical method was successfully applied to a pharmacokinetic study of the multi-components after oral administration of Sanjie Zhentong Capsule in rats.

Objective:To establish a UPLC method for simultaneous determination of 7 compounds (epidemin A1,epidemin A,epidemin B,epidemin C,icariin,2-O-rhamnosyl-icariside Ⅱ and baohuoside Ⅰ) in Yinyanghuo Zonghuangtong capsule (YZC) by quantitative analysis of multi-components with a single-marker (QAMS) and to evaluate the adaptation and application of QAMS method in the quality control of YZC.Method:Icariin was used as the internal reference substances and the relative correlation factors (f) of epimedin A1,epimedin A,epimedin B,epimedin C,rha-icariside Ⅱ and baohuoside Ⅰ were calculated.Result:The relative correlation factors (f) were 1.413,1.231,1.375,1.166,0.962 and 0.689 for epimedin A1,epimedin A,epimedin B,epimedin C,rha-icariside Ⅱ and baohuoside Ⅰ respectively.The determination results were compared with those obtained by the external standard method.The reproducibility of the established method was satisfying and there was no significant difference in assay results between the QAMS method and the external standard method.Conclusion:The QAMS method was reliable and could be used for quality control of YZC.

Objective To establish an HPLC fingerprint of Xiaochuan Granula (XCG),and to make a quantitative analysis of seven components by fused-core column.Methods Kromasil C15 (150 mm × 4.6 mm,3.5 μm) was used with the mobile phase of Methanol (A)-acetonitrile (B)-water (C),at a flow rate of 1.0 mL/min.The detection wavelength was set at 210 nm for amygdalin and magnolin,240 nm for morroniside,loganin,prim-O-glucosylcimifugin,4'-O-β-glucopyranosyl-5-O-methylvisamminol,schizandrin,and fingerprint;And the column temperature was maintained at 40 ℃.Common peaks had been identified by UPLC-Q-TOF/MS and standard compounds.Results The fingerprint chromatography included 20 mutual peaks,and the similarity was more than 0.90.Eleven chemical components were identified by UPLC-Q-TOF/MS and standard compounds,which were 3-morroniside,oxypaeoniflorin,5-1oganin,6-prim-O-glucosylcimifugin,9-4'-O-β-glucopyranosyl-5-O-methylvisamminol,11-magnolin,15-schisandrin,16-schisandrol B,18-schisantherin A,19-deoxyschizandrin,and 20-γ-schizandrin B.Moreover,seven active components (morroniside,oxypaeoniflorin,loganin,prim-O-glucosylcimifugin,4'-O-β-glucopyranosyl-5-O-methylvisamminol,magnolin,and schisandrin) were quantified and the average recovery rates ranged from 97.3％ to 103.8％ with RSDs less than 2.0％;Seven components in 10 batches samples were morroniside 0.51-0.69 mg/g,amygdalin 5.01-5.95 mg/g,loganin 1.02-1.33 mg/g,prim-O-glucosylcimifugin 0.35-0.45 mg/g,4'-O-β-glucopyranosyl-5-O-methylvisamminol 0.45-0.55 mg/g,magnolin 0.38-0.48 mg/g,schisandrin 0.89-1.08 mg/g,respectively,and RSD of each component was less than 11.0％.Conclusion The method for establishing HPLC fingerprint and quantitative analysis of seven components is rapid,simple,and accurate,and can be used for the quality control of XCG.

To establish a UPLC-MS/MS method for simultaneous determination of six triterpenoid constituents (pachymic acid, dehydropachymic acid, dehydrotumulosic acid, polyporenic acid C, dehydroeburicoic acid and dehydrotra metenolic acid) in Guizhi Fuling capsules (GFC). Chromatographic analysis was conducted on Agilent Porosheell 120 SB-C₁₈ column (4.6 mm×150 mm, 2.7 μm), with 0.1% formic acid aqueous solution-methanol as the mobile phase for gradient elution at a flow rate of 0.4 mL•min-1. The column temperature was 30 ℃ and the sample size was 5 μL. The samples were analyzed by tandem mass spectrometer with negative electrospray ionization (ESI) source, and monitored under a multiple reaction monitoring (MRM) mode, with the quantitative ion pairs m/z 527.8→465.5 (pachymic acid), m/z 525.6→465.6 (dehydropachymic acid), m/z 483.4→337.3 (dehydrotumulosic acid), m/z 481.5→419.5 (polyporenic acid C), m/z 467.4→337.1 (dehydroeburicoic acid), m/z 453.4→337.0 (dehydrotra metenolic acid). Six triterpenoid acids showed good linear relationships within the investigated concentration ranges (r> 0.996 8), with RSDs of precision less than 6.2%, and all RSDs of repeatability less than 5.9%. The average recovery rate was 97.90%, 100.2%, 99.60%, 101.7%, 102.6% and 103.0% respectively. The method was rapid, accurate, repeatable and could be used as a method for quantitative determination of triterpenoid acids in Chinese medicine prescriptions, providing a reference method for the quality control of Guizhi Fuling capsules and providing a reference for the content determination for Chinese medicine prescriptions containing Poria cocos.

目的:建立益心舒片的HPLC指纹图谱,为益心舒片的质量评价提供方法.方法:样品经75％甲醇提取后,采用Waters Symmetry ShieildTMC18色谱柱(4.6 mm ×250 mm,5μm)进行检测,以乙腈-0.5％甲酸水为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长260 nm,柱温30℃.采用国家药典委员会出版的“中药色谱指纹图谱相似度评价系统”(2012年版),对10批不同批次的益心舒片化学成分指纹图谱进行相似度计算,并且通过对照品比对及高效液相与质谱联用技术(HPLC-MS)对主要共有峰进行指认.结果:10批益心舒片指纹图谱中有29个共有峰,各峰分离度良好,各批次间共有峰的相对保留时间RSD均＜1.0％,样品间相似度均＞0.9,共指认出12个峰.结论:该方法具有良好的精密度、重复性、稳定性,各共有峰间分离度高,可用于益心舒片的质量综合评价.

Ethnopharmacological relevance: The twigs and bark of Cinnamomum cassia Presl (Lauraceae) are widely used in traditional Chinese medicine in the treatment of tumor, abdominal pain, dysmenorrhea, digestive system disease and inflammatory diseases. The aim of this study was to determine the inhibitory effect of the essential oil from the twigs of Cinnamomum cassia Presl (EOCC) on uterine contraction in vitro and in vivo.Materials and methods: The Institute of Cancer Research (ICR) mouse uterine contraction was induced by oxytocin (OT) exposure following estradiol benzoate pretreatment. Mice were given the EOCC (60, 30, and 15 mg/kg) by gavage. The level of prostaglandin F-2 alpha (PGF(2 alpha)) in uterine tissue were determined according to specification of enzyme linked immunosorbent assay (ELISA) kit. Uterine tissue was collected for histopathological analysis (H & E). Myosin light chain 20 (MLC20), phosphorylation of myosin light chain 20 (p-MLC20) and cyclooxygenase-2 (COX-2) proteins in uterine tissue were assessed by Western Blot. Mouse isolated uterus strips were mounted in tissue organ baths containing Locke's solution. The contractile responses were recorded with Power Lab recording system. The effect of the EOCC on uterine contraction induced by OT, PGF(2 alpha), and acetylcholine (Ach) was observed. Myometrial cells were exposed to OT (7 mu M) to induce Ca2+ release, and the effect of the EOCC (100, 50, and 25 mu g/ml) on intracellular Ca2+ was analysed with fluorometry imaging.Results: In vivo study demonstrated that the EOCC significantly reduced OT-induced writhing responses with a maximal inhibition of 66.5%. It also decreased the level of PGF(2 alpha) in OT-induced mice uterine tissue. Moreover, Western blot analysis showed that COX-2 and p-MLC20 expressions in uterine tissue of dysmenorrhea mice were significantly reduced. EOCC inhibited spontaneous uterus contractions in a dose-dependent manner, and the concentration of the EOCC giving 50% of maximal contraction (IC50) value was 61.3 mu g/ml. The IC50 values of the EOCC on OT, PGF(2 alpha), and Ach-induced contractions were 113.0 mu g/ml, 94.7 mu g/ml, and 61.5 mu g/ml, respectively. Further in vitro studies indicated that the EOCC could restrain intracellular Ca2+ levels in favour of uterine relaxation.Conclusion: Both in vivo and in vitro results suggest that the EOCC possesses significant spasmolytic effect on uterine contraction. Thus, the EOCC yields a possible therapeutic choice for the prevention and treatment of primary dysmenorrhea.

目的:测定不同产地不同加工方法牡丹皮药材中没食子酸、芍药内酯苷、芍药苷等7个成分及Fe,Mg等24种微量元素的含量,探讨牡丹皮药材中物质与微量元素间的关系,为牡丹皮药材的质量综合评价提供参考.方法:采用高效液相色谱波长融合方法测定没食子酸,芍药内酯苷,芍药苷,1,2,3,4,6-五没食子先葡萄糖,苯甲酸,苯甲酰芍药苷,丹皮酚7个成分的含量;采用微波消解-电感等离子体质谱法(ICP-MS)测定Fe,Mg等24种微量元素的含量;通过对指标成分、微量元素等进行相关分析并建立灰色关联度模型,综合评价不同产地不同加工方法牡丹皮的质量.结果:不同产地不同加工方法牡丹皮指标性成分及微量元素含量部分有显著差异(P＜0.05),灰色关联度模型稳定,牡丹皮中的不同成分、各种微量元素之间存在显著的或极显著的正相关或负相关(P＜0.05).结论:微量元素与多指标成分的相关性分析和灰色关联度模型为牡丹皮优质药材选择提供充分的依据和参考.

采用煎煮法对茯苓中铁、镁、铜、锌、铝等24种微量元素进行提取,水煎液经0.45 μm的微孔滤膜过滤后分为悬浮态和可溶态,对可溶态中的有机态和无机态采用LSA-10大孔树脂进行分离;采用正辛醇-水分配体系,用萃取法对其水煎液在模拟人体胃(pH=1.3)和肠(pH=7.6)环境条件下,将微量元素分离为醇溶态和水溶态,采用电感耦合等离子体质谱法对各种形态的微量元素进行含量测定,研究结果显示,方法回收率在76.7％ ～115.7％之间,精密度RSD为3.5％ ～7.8％.结果显示各微量元素的溶出率在18.5％～95.5％之间,可溶态在水中的比率在33.6％～99.9％之间,微量元素主要以无机态和水溶态的形式存在.微量元素的形态分析结果为茯苓的综合研究有重要的实际意义.

目的:建立基于QuEChERS结合超高效液相色谱-串联质谱(UPLC-MS/MS)法的三七、川贝母、薏苡仁、桂枝、金银花5种中药材中35种有机磷农药残留量的快速检测方法.方法:采用QuEChERS法对样品进行前处理,Agilent Poroshell C18色谱柱(2.7 μm,4.6 mm×150 mm),柱温35 ℃,以甲醇-0.1％(v/v)甲酸水溶液为流动相进行梯度洗脱(0～4 min,20％A;4～8 min,20％A→60％A;8～12 min,60％A→80％A;12～35 min,80％A→90％A),流速0.4 mL· min-1,进样量5 μL;采用电喷雾离子源进行正离子模式监测,多反应监测模式(MRM)用于定量分析.结果:35种农药在一定含量范围内线性关系良好,相关系数(R2)均大于0.98.在LOQ、10 LOQ和30 LOQ 3个添加浓度水平进行了添加回收率实验,平均回收率均在70.63％～110.6％之间,RSD在0.66％～14.18％之间.35种有机磷农药成分的定量限在0.12～76.5 μg· kg-1之间;50批药材样品共检出了甲胺磷、倍硫磷及毒死蜱3种农药残留,但均符合标准规定.结论:该方法操作简便快速、经济有效、灵敏可靠,适用于中药材中多种农药残留的快速筛查测定.