Objective:To develop an ultra performance liquid chromatography (UPLC) method for the determination of five synthetic pigments such as tatrazine,amaranth,carmine,sunset yellow and brilliant blue in beverage. Methods:Synthetic pigments in beverage were extracted by polyamide adsorption method,separated on a Waters ACQUITY UPLCTM BEH C18 column by gradient elution using a mobile phase made up of methanol and 0.02 mol/L ammonium acetate,and detected at 254 nm and 629 nm. Results:The five synthetic pigments were separated in 5.5 min. The UPLC method exhibited excellent linearity over a range of 3-80 mg/L (r 0.9995). The detection limit,recovery rate and relative standard deviation (RSD) of this method were 0.03-0.10 mg/L,97.4%-107.3%,and 0.22%-3.20%,respectively. Conclusion:The analytical method is simple,accurate,sensitive and suitable for the determination of pigments in beverage.

Ultra high performance liquid chromatography(UHPLC) technology is an innovative liquid chromatography technology.It takes advantages of small particle packing,low system volume and rapid detection techniques,and has very significant increases in resolution,speed,and sensitivity.This paper reviews recent applications of UHPLC in the food safety analysis,particularly in analyses of pesticide residue,veterinary drug residue,food additives,illegal additives and toxic substances.

超高效液相色谱（UHPLC）技术是在高效液相色谱（HPLC）技术的基础上,进一步利用亚微米颗粒填料、超高压输液泵、低死体积系统及快速检测等特点发展起来的分析检测技术,具有更高的分离度、分析速度和检测高灵敏度。本文综述了近年来UHPLC技术在食品安全检测中的应用,特别是在检测农药残留、兽药残留、食品添加剂、非法添加物以及有毒物质等方面取得的研究成果。