以辣木全粉和普通小麦粉为原料制备辣木全粉馒头,考察辣木粉添加量、加水量、和面时间和发酵时间对辣木全粉馒头感官品质的影响,利用Brookfield质构仪研究辣木全粉馒头的质构特性,利用激光扫描共聚焦显微镜研究辣木馒头微观结构变化.结果表明,当辣木添加量为3‰、发酵时间50 min、加水量100 mL、和面时间10 min时,馒头制品色泽度高,口感香甜,感官评分最高.辣木粉的添加能够明显改善馒头质构和微观结构,提高馒头品质.

金属-有机框架(metal-organic frameworks,MOFs)化合物是由金属离子和有机配体通过自组装而成的具有网格结构的晶态材料.由于其结构的多样性和孔道的可调节性,使其在气体的吸附和分离、离子识别、催化、磁性和生物医学等方面有着较好的应用前景.本文简单介绍了近十年来含N和羧酸混合配体构筑的MOFs化合物在设计合成和性能研究方面取得的进展,以期广大科研工作者能对MOFs材料在合成设计和性能研究方面有比较全面的认识.研究发现,在MOFs化合物的设计合成中使用含氮配体和芳香羧酸的混合配体是一种行之有效的策略,可以得到更多结构多样化和性能优良的MOFs材料.

The organic acid content in blueberry juice is an important parameter to evaluate the quality of fruit juice.Eleven kinds of organic acids(oxalic acid,acetic acid,crylic acid,succinic acid,malonic acid,tartaric acid,fumaric acid,citric acid,propionic acid,malic acid and lactic acid)in five kinds of commercial blueberry juice products were determined simultaneously by using high performance liquid chromatography-diode array detector (HPLC-DAD).The determination conditions were as follows:Hypersil C1s column (5 m,250 mm× 4.6 mm i.d.),methanol-0.5％ NaH2PO4 as mobile phase,gradient elution,injection amount 20.0 L,flow rate 1.0 mL/min,colunn temperature 25 ℃,and detection wavelenght 210 nm.The 11 kinds of organic acids were identified by retention time and characteristic spectra and quantified by peak area.Results showed that the mass concentrations of the 11 kinds of had good linear relationship with peak areas,with the correlation coefficients of 0.996 2 to 0.999 5;the The average recoveries were 86.3％ to 99.7％ with relative standard deviations of 2.2％～4.6％(n=7) and detection limits of 0.4 to 2.5 mg/L.The proposed method was used to determine commercial blueberry juice products,and results showed that the contents of the 11 kinds of organic acids in five blueberry juice products had significant difference.The method is simple,quick,sensitive,and reproducible,is suitable for organic acid content analysis in fruit juice,and is practical and instructive for evaluating the quality of blueberry juice and other related products.

采用过硫酸氢钾(Oxone试剂)催化氧化降解水中微污染物磺胺甲(口恶)唑.探究了磺胺甲(口恶)唑初始浓度、溶液pH、反应温度、Oxone试剂用量、反应时间及水中有机物腐殖酸等对磺胺甲(口恶)唑降解效果的影响,结合液相色谱质谱(LC-MS)分析阐明了磺胺甲(口恶)唑的降解路径.结果表明:磺胺甲(口恶)唑溶液初始质量浓度为15 mg/L、溶液pH 9.3、反应温度为30℃、Oxone试剂用量为3.0 mmol、SRHA的浓度为10 mg/L、且紫外灯辐照下,反应3h后,磺胺甲(口恶)唑的降解率达到了95.9％.

Xylooligosaccharides (XOS) are composed of xylose units, which widely exist in the nature of veg-etables, fruits, milk and honey and are produced by main raw material of lignocellulose in industry processing. As edible source of dietary fiber, XOS has some biological activities involving improving intestinal health, strengthening immune and promising processing performances. So it has widely used in food, feed and other fields. In this paper, the production status, preparation and the application of XOS was stated and the research improvement was also illustrated.

在高校教育活动中,实践教学承担着极为重要的作用,是培养教师教育类专业人才的重要基础,是提高学生专业技术水平与实践能力的重要保障.建设教育实践教学基地,要求充分认识教师教育实践教学基地的功能性,关注实践教学基地的现实特征,依据院校实际制定时间教学基地培育目标,加强校校合作与校地合作,不断提高教师队伍水平,搭建完善的实践教学体系,以打造高质量的实践教学基地.

In this study,Glycine max (L.)Merr is the test material.By extracting Glycine max (L.)Merr genome DNA,SRAP-PCR amplification used in Glycine max (L.)Merr,PCR products are electrophoresised in the polyacrylamide gel,and finally they are stained with silver nitrate.An effective,simple and quick PAGE silver staining method is be built by Glycine max (L.)Merr SRAP markers.

创新型国家需要创新型的人才，而在人才创新能力的培养过程中，学校教育绝对占着主导地位。而高等院校原有的教育模式，对研究生创新性思维及创新能力培养的效果和力度是远远不够的。本文从高校、导师、研究生自身三个方面着手，阐述了创新型人才培养过程中三个重要的因素。

A capillary electrophoresis-electrospray ionization mass spectrometry(CE-ESIMS)method for the simultaneous determination of oxalic,fumaric,succinic,citric,malic,ascorbic,tartaric and tartaric acid in grape wines was established.The samples were separated by an uncoated capillary(50μm×80cm)on the operating voltage of 25kV using 40.0 mmol/L ammonium acetate(pH 4.5)as the running buffer and 30%2-propanol-70% water mixture(containing 3mol/L ammonia)as the sheath liquid.The baseline separation of eight compounds was achieved within 15min with satisfactory repeatability and sensitivity.The linear ranges are 1.0—300.0,1.0—300.0,5.0—1 500.0,2.0—600.0,5.0—1 500.0,1.0—300.0,5.0—1 500.0and 5.0—1 500.0mg/L for oxalic,fumaric,succinic,citric,malic,ascorbic,tartaric and tartaric acid with detection limits of 0.2,0.05,1.0,0.5,1.0,0.3,1.0,1.0 mg/L,respectively.The average recoveries of the eight components are 87.6%—98.2% with the RSDs of 2.7%—5.6%.

A micellar electrokinetic capillary chromatographic method for fast determination of N-methylcarbamate pesticides was developed by using lauric acid buffer system.The effect of some key factors,such as concentration of lauric acid and ammonia,addition of organic solvent,capillary temperature and applied voltage on the separation was investigated.Under the optimized conditions,45 mmol/L lauric acid(dissolve in ethanol) and 120 mmol/L ammonia(pH=9.5) as the running buffer,the baseline separation of seven N-methylcarbamate pesticides was achieved within 4.5 mins.The detection limit of each analytes varied from 2.0 to 15 μg/L with the preconcentration of ODS-C18 solid phase extraction column.The average recoveries of each analytes from farm water ranged from 85% to 107% for all with RSDs below 8.3%(n=5).

In this study,12 primer combinations of SRAP with characteristics of rich polymorphism,clear bands and good repeatability were selected from 288 primer combinations for 113 accessions of cultivated soybean(Glycine max) and 20 accessions of wild soybean(Glycine soja).The 12 primer combinations were used in the PCR amplification,and the abundant and distinct DNA finger printings of PAGE were obtained.251 DNA bands were obtained,220 of which bands were polymorphic,amounted to 87.6% of the total amplified bands,and 18.3 bands were obtained with each primer,averagely.Result showed that the selected 12 primer combinations could be effectively applied to SRAP analysis of the germplasm resources of Glycine max(L.) Merr..

[Objective] To study the electrochemical behavior of antitumor herbal drug apigenin and the interaction of apigenin with DNA at glassy carbon electrode. [Method] Cyclic voltammetry （CV） and differential pulse voltammetry （DPV） were used in experiments. [Result] In CV, only one irreversible anodic peak with Ep = 0.580V was appeared in 0.1 mol/L B-R buffer solution （50% ethanol, pH 9.0）. The peak currents of apigenin were linearly related to concentrations in a range from 5.0×10-6 to 9.0×10-5 mol/L. And the detection limit was 1.5×10-6 mol/L. A new electroanalytical method for this herbal drug was established according to this anodic peak. Using the established method without preseparation, apigenin in herbal drug was determined with satisfactory results. [Conclusion] This method is a new one to detect apigenin.

The detection methods of proanthocyanidin are compared and their merits and demerits are comparatively analyzed,so as to provide basis for exploring better determination methods of proanthocyanidin.

本文采用自制催化剂固体杂多酸TiO2/SO42-通过醇交换法催化合成十二烷基糖苷,讨论了催化剂用量、原料配比、反应温度、对产品得率、反应时间和多糖含量的影响,确定较为适宜的操作工艺条件为:m(催化剂)∶m(葡萄糖)=0.015∶1,n(葡萄糖)∶n(丁醇)∶n(十二醇)=1:6:1,反应温度110～120℃,并对其结构和性能进行了分析和表征。

实验室是学生学习实践和教师科研的重要场所。结合本校实际情况,对郑州市重点专业——食品专业实验室的建设、教学内容和教学方法等方面进行了探讨,并提出看法,学生培养要符合地方经济特点和需要。

Mixed micellar electrokinetic capillary chromatography was applied to the separation and determination of sex hormones.A mixed solution containing 30 mmol·L-1 SDS,40 mmol·L-1 lauric acid,110 mmol·L-1 ammonium hydroxide and 2-propanol-water(15+85) solution was used as the electrophoretic medium.Separation voltage applied was 20 kV and wavelength of detection was taken at 214 nm.Under the optimum conditions,9 sex hormoneds was completed within 13.5 min.Values of detection limit(3S/N) of the method found were in the range of 0.2 to 2.7 mg·L-1.The proposed method was used in the analysis of sample of Penis et Testis Cervi,giving values of recovery and RSD′s(n=6) in the ranges of 85.0%-109.0% and 2.5%-5.5% respectively.

高职院校教学中开展环境教育,是高职院校环境教育发展的趋势。在对高职院校环境教育的现状及存在问题进行全面分析的基础上,提出了环境教育课程化的基本方式,包括开设环境教育课程和加强实践性环境教育活动。

The electrochemical behavior of six novel conjugated polymers containing phenothiazine moieties in main chain was studied by some electrochemical techniques,and the electrode reaction processes were investigated in detail and their dynamic parameters were obtained too.In addition,the energy levels of the frontier orbits of the six polymers were calculated and analyzed.The effect characteristics of these six polymers with IIB metal ions were investigated too.The results show that these polymers all have well defined redox peaks,and the addition of metal ions shifts the oxidation peak potential of the corresponding phenothiazine group to more cathodal position.