Aim To observe the effect of Salvianolic acid B(SalB) on atherosclerotic plaque stabilization in diabetic atherosclerosis animal models prepared from apolipoprotein E(ApoE) gene knock-out mice treated by intraperitoneal injection of STZ and high fat diet.Methods Fifty two-month female ApoE gene knock-out mice were injected with STZ and fed with high fat diet for 12 weeks.Then fasting blood glucose(FBG) level was measured and forty diabetic atherosclerosis mice were selected for the following treatment experiment.Diabetic atherosclerosis mice were randomly divided into four groups.Each group was fed with same high fat diet and intragastrically administrated with different drugs for 8 weeks,named model group(distilled water),SalB high dose group(160 mg/(kg·d)),SalB medium dose group(80mg/(kg·d)) and Lovastatin group(2.3 mg/(kg·d)).Then frozen sections and paraffin sections of mice aortas were made.The lipid core area,thickness of fibrous cap,incidence of plaque erosion and the number of neovascularization in atheromatous plaque were measured by Sudan III staining,MASSON staining and immunohistochemistry staining. Results Compared with the model group,both the level of FBG and total cholesterol(TC) in the SalB high dose group and Lovastatin group reduced significantly(P0.05).Compared with the model group,both the level of triglyceride(TG) and low density lipoprotein cholesterol(LDLC) in the SalB medium dose group,SalB high dose group and Lovastatin group reduced significantly(P0.05),whereas the level of high density lipoprotein cholesterol(HDLC) increased significantly(P0.05).Compared with the model group,lipid core area,the number of neovascularization in atheromatous plaque and the incidence of plaque erosion in the SalB medium dose group,SalB high dose group reduced significantly(P0.01),whereas average thickness of fibrous cap became thicker significantly(P0.01).Conclusions SalB may stabilize the diabetic atherosclerotic plaques by increasing average thickness of fibrous cap and decreasing lipid core area,incidence of plaque erosion and neovascularization in atheromatous plaque.

Objective:To study the effects of sallvianolic acid B on the expression of Bcl-2 and Bax proteins in ApoE-/-mice induced by high fat feed combined with STZ.Methods:The eight-week old mice were given intraperitoneal injection with stz and fed in high fat diet for 12-weeks to make diebatic atherosderosis model,then assigned them randomly into four groups:model group,atorvastatin group,high Salvianolic acid B group and middle Salvianolic acid B group.After treating 8 weeks,the immunohistochemistry were used to monitor the expression of Bcl-2 and Bax proteins in aortas and ratios of both were compared.Results:In modle group,the expression of Bcl-2 or Bax proteins was higher than those in control group,the latter was increased more obviously;and ratio of both expressions was in imbalance.In high sallvianolic acid B group,compared with model group,the expression of Bcl-2 protein was increased,while the expression of bax protein was reduced;and ratio of both was recovered.Conclusion:Sallvianolic acid B could up-regulate the expression of bcl-2 protein,down-regulates the expression of bax protein simultaneously and recovers the ratio of both,which might be one of mechanisms of sallvianolic acid B preventing and treating diabetic atherosclerosis disease.

[Objective] To observe the effect of Salvianolic acid B on thrombogenesis of atheromatous plaque in mice with diabetic atherosclerosis and the possible mechanism.[Methods] Fifty two-month female ApoE-gene knock-out mice were intraperitoneally inject-ed with 200 mg/kg STZ and fed with high fat diet for 12 months.Then blood sugar level was measured and forty diabetic atherosclerosis mice were selected for the following treatment experiment.Diabetic atherosclerosis mice were randomly divided into four groups and the mice in each group were fed with same high fat diet and administrated per oral with different drugs for 8 weeks,named MOD group(dis-tilled water),SalBH [SalB 160 mg/(kg.d)] group,SalBM group[SalB 80 mg/(kg.d)] and Lovastatin[LVT 2.3 mg/(kg.d)] group.Then fast blood sugar level(FBG) was measured by using full automatic biochemistry instrument;the concentration of blood plasminogen activator inhibitor-1(PAI-1)was determined by ELISA techniques.Mice aortas were subjected to fixation and paraffin imbedding,then paraffin sections were made.The expression of tissue factor(TF)was measured by immunohistochemistry staining and then analyzed by Image-Pro Plus(IPP) version 6.0 system.[Results] Compared with the MOD group,average optical density(OD) value of TF reduced significantly in SalBH,SalBM and LVT treatment group(P0.05);PAI-1 concentration reduced significantly in SalBM group(P0.05).[Conclusions] Salvianolic Acid B can decrease the expression of TF in atheromatous plaque and concentration of blood PAI-1.The result suggested that Salvianolic Acid B may reduce thrombogenesis by above mechanism.

[Objective] To observe the effect of Salvianolic acid B on diabetic atherosclerosis in Apolipoprotein E (ApoE)-gene knocked mice. [Methods] eight-week old mice were given intraperitoneal injection with stz and fed with high fat diet for 12-weeks to establish a diebatic atherosderosis model,then assigned them randomly into three groups:model group (fed with saline) Salvianolic acid B group (fed with Salvianolic acid B )and atorvastatin calcium positive control group (2.3 mg/kg everyday). After treating for 8 weeks,all the mice were killed and the aortas were stripped for morphological research and photograph analysis. [Results] In model group,some plaques were bigger,merged into flake,aortic wall thickened diffusively. Compared with that,In Salvianolic acid B treatment group and Lovastatin positive control group,the aortic sinus had a more limited plaque lesion. Morphological research showed that As lesions were found in aortas of model group and Salvianolic acid B group. The lesions of model group were more serious than those of Salvianolic acid B group. [Conclusion] Salvianolic acid B can relieve the diabetic As lesions of the ApoE gene knockout mice.

糖尿病(DM)是由胰岛素分泌和/或作用的缺陷所致的以慢性高血糖为特征的代谢紊乱性疾病[1].

[Objective] To observe the effect of Salvianolic acid B on diabetic atherosclerosis in Apolipoprotein E (ApoE)-gene knocked mice. [Methods] eight-week old mice were given intraperitoneal injection with stz and fed with high fat diet for 12-weeks to establish a diebatic atherosderosis model,then assigned them randomly into three groups:model group (fed with saline) Salvianolic acid B group (fed with Salvianolic acid B )and atorvastatin calcium positive control group (2.3 mg/kg everyday). After treating for 8 weeks,all the mice were killed and the aortas were stripped for morphological research and photograph analysis. [Results] In model group,some plaques were bigger,merged into flake,aortic wall thickened diffusively. Compared with that,In Salvianolic acid B treatment group and Lovastatin positive control group,the aortic sinus had a more limited plaque lesion. Morphological research showed that As lesions were found in aortas of model group and Salvianolic acid B group. The lesions of model group were more serious than those of Salvianolic acid B group. [Conclusion] Salvianolic acid B can relieve the diabetic As lesions of the ApoE gene knockout mice.