This study systematically collected, analyzed, and evaluated randomized controlled trial(RCT) in the treatment of diabetic foot ulcer(DFU). The aim as provide references for future studies and to enhance the application of clinical evidence. The RCT of DFU treated with Chinese Patent Medicine was obtained and analyzed using the AI-Clinical Evidence Database of Chinese Patent Medicine(AICED-CPM). The analysis was supplemented with data from CNKI, Wanfang, VIP, SinoMed, PubMed, EMbase, Cochrane Library, and Web of Science. A total of 275 RCTs meeting the requirements were retrieved, with only 7 of them having a sample size of 200 or more. These trials involved 66 different Chinese patent medicine including 25 oral medications, 24 Chinese herbal injections, and 17 external drugs. Among the 33 different intervention/control designs identified, the most common design was Chinese patent medicine + conventional treatment vs conventional treatment(86 cases, 31.27%). Out of the 275 articles included in the literature, 50 did not provide information on the specific course of treatment(18.18%). A total of 10 counting indicators(with a frequency of 426) and 36 measuring indicators(with a frequency of 962) were utilized. The methodological quality of the RCT for the treatment of DFU with Chinese patent medicine was found to be low, with deficiencies in blind methods, other bias factors, study registration, and sample size estimation. There were noticeable shortcomings in the reporting of allocation hiding and implementation bias(blind method application). More studies should prioritize trial registration, program design, and strict quality control during implementation to provide valuable data for clinical practice and serve as a reference for future investigations.
Objective To analyze clinical efficacy of moxibustion therapy in the treatment of diabetic peripheral neuropathy.Methods According to the random number method,120 patients with diabetic peripheral neuropathy were selected in our hospital from June 2015 to October 2016 and were divided into control group (n=60) and observation group (n=60).The control group was treated with Mecobalamin oral drug treatment,and the observation group was treated with moxibustion.The sensory and motor nerve conduction velocities were measured before and after the treatment by electromyography,and the SF-36 quality of life scale (BP) score,VAS score and the effective rate of the treatment were recorded.Results Before treatment,the motor and sensory nerve conduction velocity of two groups had no significant difference (P>0.05);After treatment,motor and sensory nerve conduction velocity of two groups were significantly improved,but above indicators of the observation group was significantly better than the control group (P<0.05).Before treatment,VAS score and BP score of two groups had no significant difference (P>0.05);After treatment,VAS score and BP score for both groups were improved,but the observation group improved more than the control group (P<0.05).The total effective rate of the observation group was 85%,which was higher than 68.33% of the control group (P<0.05).Conclusion Moxibustion therapy in the treatment of diabetic peripheral neuropathy highlights the advantages of Chinese medicine,it can reduce injury of peripheral nerve in diabetic patients,improve the clinical treatment effect and the quality of life of patients,so it has better application prospect.
[Objective]A Meta-analysis of the efficacy and safety of tonifying kidney and promoting blood circulation in the treatment of chronic kidney disease.[Methods]By searching CNKI,VIP,Wanfang Data,SinoMed,PubMed,Web of Science databases,the kidney tonifying and blood circulation method combined with conventional Western medicine treatment was used as the test group,and the conventional Western medicine treatment group was used as the control group,and the related words such as"kidney and blood circulation","chronic kidney disease"were used as the search terms,and the randomized controlled trials were used as the retrieval targets.Relevant studies meeting the inclusion criteria were evaluated by the"risk of bias assessment"tool recommended by Cochrane Reviewer's Handbook 5.0.1,and the data were analyzed by Revman 5.4.1 software.[Results]In the 18 articles included,taking the clinical efficiency,blood creatinine,urea nitrogen,uric acid,glomerular filtration rate,severity of adverse reactions as outcome indicators,it was concluded that the efficacy of the experimental group was higher than that of the control group,with statistical difference(P<0.05).[Conclusion]The method of tonifying kidney and activating blood circulation has curative effect on chronic kidney disease.
[Objective]To investigate the protective effect and possible mechanism of Ciwujia Injection(CWJI)on myocardial tissue of rats with heart failure after acute myocardial infarction(HF-AMI).[Methods]The rat model with HF-AMI was established by ligation of the anterior descending branch of the left coronary artery,and model group,Captopril(CPT,10 mg/kg)group,CWJI low-dose(CWJI-L,15 mg/kg),medium-dose(CWJI-M,30 mg/kg),high-dose(CWJI-H,60 mg/kg)group,and the Sham group were set up,with 16 rats in each group.After 4 weeks of continuous administration once a day in each group,the cardiac function[left ventricular ejection fraction(LVEF),left ventricular short axis shortening rate(LVFS),maximum rate of rise and fall of left indoor pressure(+dp/dtmax,-dp/dtmax)]was detected by Doppler ultrasound and physiological signal acquisition system.The level of cardiac troponin(cTnI),angiotensinⅡ(AngⅡ)and aldosterone(ALD)in serum were determined by enzyme-linked immunosorbent assay(ELISA).The left ventricular myocardial hypertrophy index(LVHI)was calculated.The myocardial histopathological changes,apoptosis and tissue fibrosis were observed by HE,TUNEL or Masson staining,the mitochondrial ultrastructure changes was observed by transmission electron microscopy.The activity of total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA),reactive oxygen species(ROS)were detected by spectrophotometry.The mRNA expression of nuclear factor E2-related factor 2(Nrf2)and heme oxygenase 1(HO-1)were detected by RT-PCR.The protein expression of Nrf2 and HO-1 were detected by Western Blot.[Results]Compared with the model group,the LVEF,LVFS,+dp/dtmax,-dp/dtmax,the level of cTnI,AngⅡ,ALD in serum and LVHI in CPT group,CWJI-M,CWJI-H groups were significantly decreased(P<0.05).The myocardial histopathological changes,apoptosis and tissue fibrosis were significantly improved,and the apoptosis index(AI)and collagen volume fraction(CVF)were significantly decreased(P<0.05).The ultrastructural changes of myocardial cell mitochondria were significantly improved.The activity of T-SOD,GSH-Px in myocardial tissue were significantly increased,while the content of MDA,ROS were significantly decreased(P<0.05).The mRNA and protein expression of Nrf2,HO-1 were significantly increased(P<0.05).The above effects of CWJI were dose-dependent,and the effect of CWJI-H group on other indexes except LVFS was better than those of CPT group(P<0.05).[Conclusion]CWJI can alleviate myocardial tissue lesion,cell apoptosis,tissue fibrosis and mitochondrial ultrastructure lesion in rats with HF-AMI,and improve cardiac function,which may be related to activation of Nrf2/HO-1 signaling pathway and inhibition of oxidative stress.
[Objective]To explore the pharmacodynamic material basis and mechanism of Sophorae Tonkinensis Radix et Rhizoma against liver cancer based on ultra-high pressure liquid chromatography tandem four-stage rod time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)technique,network pharmacology and molecular docking.[Methods]UPLC-Q-TOF/MS technique was used to quickly identify the chemical components of Sophorae Tonkinensis.Pharm Mapper and Swiss Target Prediction were used to collect and predict component targets,followed by the search of liver cancer-related targets with GeneCards and DrugBank.The intersection targets of components of Sophorae Tonkinensis and liver cancer were obtained using Venny 2.1.0.Cytoscape 3.7.2 was used to construct the"chemical component-target-disease"network,to screen potential active components of Sophorae Tonkinensis against liver cancer.Protein-protein interaction(PPI)network was constructed using STRING to uncover the core targets of Sophorae Tonkinensis against liver cancer.,which were subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis based on DAVID database.and the"chemical component-target-pathway"network was constructed.The binding activity of the active components to the target proteins of Sophorae Tonkinensis against liver cancer and core target proteins was verified by molecular docking technology.Verify the anti hepatoma effect of Trifolirhizin,Oxymatrine and Matrine by cell activity test.[Results]UPLC-Q-TOF/MS revealed 23 ingredients of Sophorae Tonkinensis and a total of 33 intersection targets of Sophorae Tonkinensis components and the disease were obtained,PPI,GO,and KEGG analyses showed that Sophorae Tonkinensis presumedly acted on cyclin D1(CCND1),serine/threonine kinase 1(AKT1),receptor tyrosine kinase 2(ERBB2),epidermal growth factor receptor(EGFR),estrogen receptor 1(ESR1),and other targets through active components such as matrine,oxymatrine,6,8-diprenylkaempferol,syringaresinol and shandougenine A,and regulated hypoxia-inducible factor(HIF-1),receptor for advanced glucation end products(AGE-RAGE),phosphatidylinositol 3-kinase and protein kinase B(PI3K/AKT),receptor tyrosine kinase(ErbB),and other signaling pathways to play the role of anti hepatoma.Molecular docking verified a good binding ability of the potential active ingredients of Sophorae Tonkinensis to the core targets.Cell activity test showed that Trifolirhizin,Oxymatrine and Matrine had different anti hepatoma effects.[Conclusion]This study preliminarily indicates that Sophorae Tonkinensis is effective in playing the role of anti hepatoma through multiple components,multiple targets,and multiple pathways.This study provides a reference for elucidating the pharmacodynamic material basis and mechanism research of Sophorae Tonkinensis against liver cancer.
The evil thought of"poison"originated from Huangdi Neijing,which laid the theoretical basis of"poison"pathogenic.Rheumatoid arthritis(RA)is a common and refractory rheumatic disease in clinical practice.By combing the theory of"poison"in Huangdi Neijing and related ancient books,combined with modern medical concepts,the prevention and treatment of RA from the perspective of"Double-toxin theory"is expoused.The"Double-toxin theory"includes exogenous toxin and endogenous toxin.Exogenous toxin refers to the toxin that enters the body from outside to inside and causes diseases,including the six evils and harmful substances in the environment.Endogenous virulence refers to the virulence produced from within or latent in the body and then onset.Exogenous pathogens and endogenous pathogens breed each other,cause and effect each other,and help each other to abuse each other,leading to the occurrence of diseases together.Hoping from the aspect of"Double-toxin theory"provides new way for the diagnosis and treatment of RA.
[Objective]To systematically evaluate the efficacy and safety of treatment of children with Tourette's syndrome by traditional Chinese medicine.[Methods]6 databases of China National Knowledge Infrastructure(CNKI),Wanfang Data,SinoMed,PubMed,the Cochrane Library and Embase were comprehensively searched by computer to collect randomized controlled trials of treatment of children with Tourette's syndrome by traditional Chinese medicine.The retrieval time was from these databases inception to April 30,2022.RevMan 5.4.1 software was used for data analysis after independent literature screening,data extraction,and bias risk assessment according to pre-established inclusion and exclusion criterias.[Results]Finally,16 RCTs with 1 371 patients were included.Meta-analysis showed that traditional Chinese medicine in the treatment of pediatric tics can improve clinical efficacy,[OR=2.22,95%CI(1.58,3.13),P<0.01],improved the YGTSS total score[MD=-3.70,95%CI(-4.99,-2.42),P<0.01],YGTSS motor tic activity score[MD=-2.50,95%CI(-3.19,-1.81),P<0.01],YGTSS vocal tic score[MD=-1.56,95%CI(-2.38,-0.75),P<0.01],reduce the TSSS score[MD=0.08,95%CI(0.03,0.13),P<0.01],improve the ISLQ score[MD=5.30,95%CI(0.33,10.27),P<0.01],reduce the score of traditional Chinese medicine syndrome,and have good safety[OR=0.17,95%CI(0.09,0.31),P<0.01].[Conclusion]Traditional Chinese medicine in the treatment of Tourette's syndrome in children can improve clinical efficacy,improve motor tic and vocal tic symptoms,improve children's quality of life,reduce traditional Chinese medicine syndrome score.And it has good clinical safety.Due to the limitation of the quality of the included ariticles,high-quality clinical studies are needed for validation the conclusion.
[Objective]To analyze the effects of Tongqiao Biyan Granule combined with antihistamine in the treatment of allergic rhinitis patients(AR)and its influence on clinical symptoms and quality of life.[Methods]The 126 AR patients admitted to our hospital from April 2019 to May 2021 were selected.They were divided into control group and observation group by random grouping method,63 cases in each group.The control group was treated with aztrostine hydrochloride,the observation group was given Tongqiao Biyan Granule on the basis of control group.Clinical symptoms,naso-conjunctivitis related quality of life questionnaire(RQLQ)score,serum trace element levels and adverse reactions were compared between two groups.[Results]Compared with before treatment,the symptoms of nasal congestion,nasal itching,sneezing and runny nose in 2 groups were significantly improved after treatment(P<0.05),and the improvement degree of observation group was significantly better than control group(P<0.05).Compared with before treatment,zinc content in 2 groups was significantly increased(P<0.05);copper content and RQLQ score were significantly decreased(P<0.05),and zinc content in observation group was significantly higher than that in control group(P<0.05);copper content and RQLQ score were significantly lower than that in control group(P<0.05).There was no significant difference in the incidence of adverse reactions between 2 groups(P＞0.05).[Conclusion]Tongqiao Biyan Granule combined with antihistamine has good efficacy in the treatment of AR patients,which can improve the clinical symptoms and quality of life of patients,and regulate the level of serum trace elements,with high safety.
[Objective]To investigate the protective effect of Shengmai Capsule on colon mucosal damage induced by 5-fluorouracil(5-FU)in mice.[Methods]Twenty male Balb/c mice were randomly divided into normal group,model group and Shengmai group.The mice in model group and Shengmai group received intraperitoneal injection of 5-FU(25 mg/kg)to induce colon mucosal damage.Then,mice in normal group and the model group were given an equal amount of distilled water by gavage,while mice in Shengmai group was given an equal amount of Shengmai capsule suspension(350 mg/kg)by gavage every day.The body weight and diarrhea of mice were monitored every day;the colon length of mice was measured;the activity of diamine oxidase(DAO)in mice serum was detected;histopathological changes of colon were observed by HE(Hematoxylin-eosin staining)staining;the expression level of ki67 in colon tissue was detected by immunohistochemistry;The number of goblet cells in colon tissue was detected by PAS(Periodic Acid Schiff stain)staining.[Results]Compared with the model group,the diarrhea of mice in Shengmai group was improved significantly;the length of colon increased significantly(P<0.05);The activity of DAO in serum decreased(P<0.01);the structure of colon mucosa was improved obviously;the expression of ki67 and the number of goblet cells in colon tissue increased(P<0.000 1).[Conclusion]Shengmai capsule has obviously protective effect on colon mucosal damage induced by 5-FU.
Academic Summary of Famous Traditional Chinese Medicine(TCM)Masters in Jingu is a series of medical books to discuss the academic characteristics of famous TCM Masters.The book consists of four volumes,including the academic thoughts and clinical experience of 127 famous TCM Masters in Jingu.By analyzing the characteristics of the 11 doctors using Xiaochaihu Decoction in the first two volumes,it is found that their understanding of the efficacy of Xiaochaihu Decoction is comprehensive,with the reconciliation pivot as the core,and emphasizing the multiple functions of eliminating evils and strengthening the body resistance,dredging the Sanjiao,dredging the channel and so on.Their application of indications has a variety,widely used in Shaoyang disease,pathogenesis of pivot dysfunction or Shaoyang meridian symptoms of various diseases.Their understanding of prescription meaning has characteristics,pay attention to clear the Sanjiao,while taking into account symptomatic treatment,strengthening the body resistance and harmonizing the stomach,fully reflect the overall concept.Taking this as an example can provide a demonstration for exploring the academic characteristics of famous TCM Masters in modern Jingu and their advantages in treating diseases.
[目的]儿童流行性感冒迄今为止尚无规范的中医分型,通过调查 2019-2020 年冬春季中国儿童流行性感冒中医证候分布规律,为临床精准辨证施治提供参考.[方法]在文献研究及德尔菲法专家问卷的基础上,制定"儿童流行性感冒中医证候调查问卷",采用分层简单随机抽样的横断面调查方法,对全国 6 大行政区域 18 家三甲医院进行问卷调查,分析总结不同年龄、不同流感类型、不同区域患儿中医证型分布特点及各证型患儿症状特点.[结果]9 472 例患儿中,1)风热犯卫证占 46.63%,表寒里热证 23.41%,毒热内陷证 11.07%,风寒束表证 8.12%,邪犯肺胃证 5.87%,热毒袭肺证 4.88%,正虚邪恋证 0.02%.2)超高热占 4.30%,高热 44.52%,中度发热 44.37%,低热6.79%.3)甲型流感占 90.51%,乙型流感 9.49%,均以风热犯卫证为主(分别占 46.44%、48.50%).4)在发热的不同病程阶段,均以风热犯卫证、表寒里热证为多见,随着发热时间的推移风寒束表证比例逐渐下降,热毒袭肺证比例逐渐上升.5)各年龄段均以风热犯卫证、表寒里热证多见,各区域中医证候均以风热犯卫证为主.[结论]2019-2020 年中国儿童流行性感冒以甲型流感为主,中医证型以风热犯卫证、表寒里热证为主;发热为各证型的核心症状,毒热内陷证以高热及超高热为主,其他证型以中高度热为主;不同年龄、不同类型及不同区域中医证型分布均以风热犯卫证为主.
Anxiety insomnia belongs to the category of"insomnia"and"palpitation"in traditional Chinese medicine,and its pathogenesis is the three yin and three yang axis adverse,yin and yang imbalance caused by"Muyu".The initial illness is in the qi,due to the imbalance of the qi mechanism,the abnormal rise and fall of the five internal organs,and the disorder of the qi and blood,resulting in insomnia.Long-term illnesses enter the collaterals,stagnation of blood stasis;if the qi of the viscera is not adjusted,and the stagnation of blood stasis is not resolved,then there is no metamorphosis of ying and wei,and deficiency transformed from excess and then led to insomnia syndrome with deficiency in origin and excess in superficiality."Dazhi"takes mediating Jueyin and Shaoyang cardinal mechanism and transferring three yin and three yang as the great method.At the initial stage,it opens up the qi mechanism,harmonizes the viscera,and enters the ying blood after a long illness,so as to remove blood stasis and nourish blood and harmonize the ying and wei.If the cardinal mechanism is beneficial,yin and yang intersect,qi mechanism is smooth,and ying and wei are harmonious,insomnia syndrome can be healed.Following with one case.
Functional dyspepsia is a frequently occurring and common disease in clinical practice.In our long-term clinical practice,we believe that liver cold is one of the important pathogenesis,and some patients can receive good curative effect from liver cold treatment.This article focused on the shape and quality of liver,liver gasification,the pathogenesis of liver cold,the treatment principles and methods of liver cold,and introduced the commonly clinical experience,in order to provide readers with references to discuss.
[Objective]To explore the impacts of resveratrol(RES)on chondrocyte apoptosis in rats with temporomandibular joint osteoarthritis(TMJOA)and its regulatory mechanism on SDF-1/CXCR4 axis in this process.[Methods]Thirty-six Wistar male rats were randomly grouped into control group,model group,CXCR4 inhibitor group(AMD3100 group),RES low dose group,RES high dose group,and RES high dose+recombinant SDF-1 group(RES high+SDF-1 group),with 6 rats in each group.Histopathological changes of cartilage were observed by hematoxylin-eosin(HE)and safranine O-solid green staining;chondrocytes were isolated and cultured;cell apoptosis was detected by flow cytometry;enzyme-linked immunosorbent assay(ELISA)was applied to detect the levels of nitric oxide synthase(iNOS),nitric oxide(NO)and cytochrome C(Cyt C)in cells;Western blot was applied to detect the expression of caspase-9,Bcl-2,Bax,SDF-1,CXCR4 proteins in chondrocytes.[Results]Compared with the control group,the cartilage tissue structure of the model group was disordered and the number of cells was reduced.The staining of cartilage was obviously reduced.The injury score,cell apoptosis rate,iNOS,NO,Cyt C levels and the expression of caspase-9,Bax,SDF-1,CXCR4 proteins increased.The expression of Bcl-2 protein decreased(P<0.05).Compared with the model group,the structure level of cartilage tissue in AMD3100 group and RES low and high dose groups were gradually obvious.The number of cells increased,and the staining of cartilage increased.The injury score,apoptosis rate,iNOS,NO,Cyt C levels and the expression of caspase-9,Bax,SDF-1,CXCR4 proteins decreased,and the expression of Bcl-2 protein increased(P<0.05).Further addition of recombinant protein SDF-1 showed that the increased expression of SDF-1 reversed the inhibitory effects of high-dose RES on signal pathway and chondrocyte apoptosis(P<0.05).[Conclusion]RES can inhibit chondrocyte apoptosis in TMJOA rats by blocking SDF-1/CXCR4 signal pathway.
Danqi Piantan Capsule has the effects of benefiting qi and activating blood circulation,inducing resuscitation,activating blood circulation and dredging collaterals,used in the recovery period of meridians of ischemic stroke of qi deficiency and blood stasis type.The main ingredients are astragaloside A,salvianolic acid B,paeoniflorin,Z-ligustilide,borneol,α-asarone,β-asarone,which exert anti stroke effects by acting on multiple targets and pathways.In animal experiments and clinical studies,it has been confirmed that Danqi Piantan Capsule is involved in the treatment of stroke and complications,and its effects are related to anti-inflammatory,anti-apoptotic,neuroprotective and nerve regeneration,antioxidant stress,increased cerebral blood flow,and improved cognitive function.This article reviews the clinical research and basic research of Danqi Piantan Capsule in the treatment of stroke and complications,in order to provide a theoretical basis for the further development and utilization.
[Objective]To investigate the effects of Shugan Zishen Formula on sex hormone levels and IVF-ET treatment outcomes in patients with decreased ovarian reserve function of liver and kidney deficiency type.[Methods]A total of 98 patients with decreased ovarian reserve function were selected from the hospital and enrolled from November 2020 to November 2021.The grouping method was random number table method.The 49 patients were in the Western medicine group and received IVF-ET treatment,while 49 patients were in the Shugan Zishen Formula group and were treated with Shugan Zishen Formula on the basis of the Western medicine group.Sex hormone levels[follicle-stimulating hormone(FSH),luteinizing hormone(LH)],growth differentiation factor-9(GDF-9),anti-Muller's tube hormone(AMH)and Toll-like receptor 2(TLR2)before and after treatment were compared between the two groups.Ovarian status[peak systolic flow rate of ovarian artery(PSV),number of follicles in ovarian sinus(AFC)];traditional Chinese medicine(TCM)syndrome score before and after treatment;The dosage of gonadotropin(Gn),egg rate of MII,number of harvested follicles,fertilization rate,clinical pregnancy rate,abortion rate and adverse reactions were recorded in the two groups.[Results]After treatment,the contents of LH and FSH in Shugan Zishen Formula group were significantly lower than those in Western medicine group,and the contents of AMH were significantly higher than those in Western medicine group,with statistical significance(P<0.05).The TLR2 content of Shugan Zishen Formula group was significantly lower than that of Western medicine group,and GDF-9 level was significantly higher than that of Western medicine group,with statistical significance(P<0.05).PSV and AFC in Shugan Zishen Formula group were significantly higher than those in Western medicine group,and the difference was statistically significant(P<0.05).The TCM syndrome score of Shugan Zishen Formula group was significantly lower than that of Western medicine group,and the difference was statistically significant(P<0.05).The dose of Gn used in Shugan Zishen Formula group was lower than that in western medicine group,and the number of follicles obtained was more than that in western medicine group,with statistical significance(P<0.05);the fertilization rate,MII egg rate and clinical pregnancy rate in Shugan Zishen Formula group were higher than those in Western medicine group,with statistical significance(P<0.05);there was no statistical significance in abortion rate between the two groups(P<0.05).There was no obvious adverse reaction in both groups during treatment.[Conclusion]In the treatment of patients with decreased ovarian reserve function of liver and kidney deficiency type,Shugan Zishen Formula can adjust sex hormone levels,inhibit body inflammation,improve ovarian microenvironment,enhance ovarian reserve function,relieve clinical symptoms,reduce the dosage of Gn,increase fertilization rate,MII egg rate and clinical pregnancy rate,and improve the outcome of IVF-ET treatment.
Since the Song,Jin and Yuan Dynasties,the theories of monarch fire,ministerial fire and life-gate have gradually become a hot topic for many doctors.SUN Yikui and ZHANG Jiebin reported to their predecessors,and systematically expounded the controversial phase fire theory and the life-gate theory,which further enriched the theory of fire.This paper analyzed the academic thoughts of SUN Yikui and ZHANG Jiebin on monarch fire and ministerial fire,life-gate and ministerial fire respectively from three aspects of physiology,pathology and clinical treatment.The article found that both SUN and ZHANG were influenced by the"Neo-Confucianism Taiji"ideology and incorporated it into their theory of life-gate.Both of them emphasized the importance of warming and nourishing the qi,but with different focuses.SUN focused on enhancing the original energy while ZHANG paid attention to the complementarity of yin and yang.In addition,this paper revealed the formation process of monarch fire and ministerial fire and their relationship with the theory of life-gate,and clarified the concepts of monarch fire,ministerial fire,and life-gate.Through this study,we hope to gain a deeper understanding of their thoughts of fire theory,grasp the connection between their academic views,and it is helpful to further understand the evolution of fire theory,for the later doctors to further understand and apply the fire theory to provide certain theoretical support.
Gout is a heterogeneous group of diseases caused by abnormal purine metabolism and(or)impaired excretion of uric acid.Alkaloids are a class of organic compounds containing nitrogen that are widely found in plants and used for inflammatory reactions caused by many diseases such as gout.By consulting the latest relevant literature reports at home and abroad,this paper summarizes the research progress of the effect and mechanism of alkaloids against gout,in order to provide scientific basis and theoretical guidance for further research and clinical application of alkaloids.
[Objective]To explore the therapeutic effect of crocin(CRO)on pneumonia in young mice and its relationship with mitogen activated protein kinase(MAPK)/nuclear factor-κB(NF-κB)pathway.[Methods]A young mouse pneumonia model was constructed by intraperitoneal injection of lipopolysaccharide(LPS).The mice were randomly grouped into control group,model group,DEX(positive control dexamethasone)group,CRO low-dose group,CRO medium-dose group,CRO high-dose group,and MAPK activator(Anisomycin group,CRO high-dose+Anisomycin).The wet to dry(W/D)ratio and myeloperoxidase(MPO)activity of mouse lungs were detected.Hematoxylin eosin(HE)staining was applied to observe pathological changes in lung tissue and pathological injury score was performed.Blood cell counters and Giemsa staining were applied to perform total cell,neutrophil,and white blood cell counts.Enzyme-linked immunosorbent assay(ELISA)was applied to detect levels of inflammatory cytokines in serum and alveolar lavage fluid(BALF).Kits were applied to detect malondialdehyde(MDA)and superoxide dismutase(SOD)in serum and BALF.Western blot was applied to detect the expression of MAPK/NF-κB pathway protein.[Results]Compared with the model group,the DEX group and CRO low,medium,and high dose groups showed reduced lung tissue injury,the W/D ratio,MPO activity,pathological injury score,total cells,neutrophils,white blood cell count,IL-1β,IL-6,TNF-α,MDA,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65,p-IκBα/IκBα were obviously decreased,SOD was obviously increased(P<0.05).Anisomycin was able to reverse the therapeutic effect of high-dose CRO on pneumonia in mice(P<0.05).[Conclusion]CRO has therapeutic effects on young pneumonia mice,and its mechanism may be related to inhibiting the MAPK/NF-κB pathway.
[Objective]To establish and evaluate a clinical model for the prognosis of ischemic stroke patients treated by acupuncture.[Methods]We selected patients from February 2021 to April 2022 in the residential department of First Teaching Hospital of Tianjin University of Traditional Chinese Medicine.Combined with the patient's demographic data,past medical history,admission and treatment factors,the Logistic regression model was constructed based on LASSO regression screening predictors.The nomogram was used to visualize the model.[Results]In this study,the model was established based on age,history of hypertension,rehabilitation treatment,and the number of acupuncture within three months to predict the improvement of the modified Rankin scale at 3 months.The model is internally verified using the Bootstrap method.The area under the ROC curve of the model is 0.747,and the P-value of the Hosmer-Lemeshow goodness-of-fit test is 0.52.DOC clinical decision curve was 29%～88%.It shows that the model has good accuracy,discrimination,calibration and clinical benefit.[Conclusion]The prognostic model for acupuncture method of ischemic stroke based on LASSO regression model has good clinical application value.
