In this study, we used the leaves of Allium ampeloprasum to report on biogenic supported silver nanoparticles (AgNPs). The leaves of Allium ampeloprasum were utilized as a green reducing agent and a superior stabilizer for the AgNPs that were synthesized. The as-synthesized AgNPs were physicochemically characterized by Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), UV-Vis, and energy dispersive X-ray (EDX). The antioxidant activity of the AgNPs was assessed using the DPPH assay; they showed significant antioxidant activity, as per the IC 50 value. Because of their antioxidant qualities, recent nanoparticles seem to have an anti-human breast carcinoma effect. AgNPs that were biologically produced were evaluated for their anti-breast cancer properties against breast cancer cell lines. According to the MTT assay results, AgNPs’ anti-breast cancer qualities could effectively eradicate the MCF10 cancer cells, depending on both concentration and time. AgNPs induce cell death, followed by downregulation of the anti-apoptotic marker BCL-2 and overexpression of the pro-apoptotic markers BAX and cleaved caspase-8. Furthermore, in contrast to their corresponding control, Ag NPs prevented colony formation. More significantly, the investigation of molecular pathways of cells treated with AgNPs showed that increasing p53 expression. This implies that the extract’s pharmacological effects on human breast cancer cells were mostly caused by p53.

Whether a laparoscopically harvested omental flap is adequate for total breast reconstruction could not be determined preoperativaly due to lack of reliable assessment methods. This study aimed to establish a statistical model to predict the probability of omental flap insufficiency. In this study, 200 female patients with breast cancer receiving immediate breast reconstruction with pure pedicled omental flaps or pedicled omental flaps combined with implants after nipple-areolar complex-sparing mastectomy were divided into two groups depending on whether implants were needed or not. The clinical characteristics of these two groups were compared. Correlation of body mass index (BMI) and omental volume was analyzed. Binary logistic regression was performed to predict the probability of implant requirement based on clinical parameters, showing significant differences between the two groups. The patients who needed implants in adjunct treatment were younger. In addition, they had larger breast specimens and smaller omental volumes than the others whose omental flaps were sufficient for total breast reconstruction. Body mass index and omental volume showed a moderately positive correlation. Age, specimen volume, and BMI all were entered into the logistic regression equation. For the patients with a BMI lower than 24.0 kg/m2, the probability of requiring implants was 5.467 times that of comparable patients with a BMI of 24.0 kg/m2 or higher. At the cutoff of 0.61, the regression equation yielded a sensitivity of 84.2

Objective:To investigate the effect of radiotherapy on breast reconstruction using the pedicled omental flap combined with a silicone implant in experimental dogs.Methods:Twenty healthy local dogs were used for the experiment. Each experimental dog underwent 2 types of breast reconstruction in anterior ventral breasts. They underwent breast reconstruction using a pedicled omental flap and a silicone implant in the left breast (the omental flap group), and prosthetic breast reconstruction only with a silicone implant in the right breast (the implant group). Four weeks after surgery, radiotherapy of the same dose was given for both breasts. Eight months after radiotherapy, the omental flap, implants and capsules were removed from the dogs. The rate of capsular contracture was compared between groups by paired χ2 test (McNemar test). The thickness of capsule and microvascular density were compared between groups by paired-samples t test.Results:The capsular contracture rate in the implant group and the omental flap group was 25.0% (5/20) and 5.0% (1/20), respectively, indicating no significant difference (χ2=2.670, P=0.219). Thickness of the capsule in the implant group was significantly higher than that in the omental flap group [ (1.4±0.2) mm vs (0.8±0.1) mm, t=4.173, P=0.001]. Microvascular density in the omental flap group was significantly higher than that in the implant group (vessels in each high power field: 44.3±5.1 vs 28.4±2.8, t=5.202, P<0.001).Conclusion:Radiotherapy shows little effect on breast reconstruction with a pedicled omental flap and a silicone implant in experimental dogs and the omentum has a protective effect on the silicone implant.

Bone metastasis of breast cancer considerably reduces not only overall survival but also health-related quality of life due to pain, fatigue, and skeletal-related events. Objective: This study aims to analyze the research hotspots and trends of global research on bone metastasis of breast cancer in the past 20 years to provide a reference for relevant personnel in this field to carry out academic research. Methods: The literature related to bone metastasis of breast cancer from 2002 to 2021 was retrieved from the Web of Science. The bibliometric research method and VOSviewer and CiteSpace were used to analyze the publications, and the research status and development direction in the last 20 years were visualized. Results: A total of 7381 articles were included. The number of global publications is increasing every year. The United States contributes the most to global research, with the most citations and the highest H-index. The journal Cancer Research published the most articles on this issue. “Macrophage” and “skeletal related event” will receive more attention and be the next popular hotspot in the future. Conclusion: There will be an increasing number of publications on bone metastasis of breast cancer based on current global trends. The United States made the largest contribution to this field. More focus will be placed on the mechanisms of metastasis research, which may be the next popular topic in bone metastasis of breast cancer.

Breast cancer, the most prevalent malignancy in women, is also the leading cause of cancer-related deaths in women worldwide. The activation of the Wnt pathway plays a pivotal role in the metastatic abilities of breast cancer. In this study, IL1F6, MRGPRX1, and SEC14L3 were significantly correlated to breast cancer patients'overall survival based on TCGA-BRCA dataset. Although IL1F6, MRGPRX1 and SEC14L3 high expression were associated with better survival in breast cancer patients, SEC14L3 had the biggest survival benefit for breast cancer; therefore, SEC14L3 was selected for the subsequent investigation. SEC14L3 mRNA expression and protein levels within breast cancer cell lines decreased compared with normal human breast epithelial cells. Overexpressing SEC14L3 in breast cancer cells inhibited the malignant phenotypes of cancer cells, including the capacity of cells to migrate and invade. SEC14L3 overexpression decreased the levels of mesenchymal markers, whereas SEC14L3 knockdown facilitated the malignant behaviors of breast cancer cells. SEC14L3 overexpression also inhibited Wnt/β-catenin activation. The Wnt agonist strengthened the malignant phenotypes of breast cancer cells; moreover, the anti-tumor effects of SEC14L3 overexpression were partially attenuated by the Wnt agonist. Conclusively, SEC14L3, which is underexpressed in breast cancer cells and tissues, could play a tumor-suppressive role in a Wnt/β-catenin-related way.

目的:探讨乳管镜在诊治未扪及肿块乳头溢液中的应用价值.方法:收集2015年1-10月笔者所在医院119例非哺乳期未扪及肿块的乳头溢液患者的临床资料,总结乳管镜在诊疗乳头溢液患者中的作用.结果:95例(79.8%)患者发现了乳管内肿物,其中诊断为导管内乳头状瘤50例,导管扩张并炎症23例,乳腺增生11例,导管内癌11例.结论:乳管镜检查为未扪及肿块乳头溢液病因诊断的首选方法,对乳腺癌的早期发现有重要价值.

Objective To discuss the feasibility and effect of immediate breast reconstruction with pedicled greater omentum and gel prosthesis following nipple and areola complex sparing mastectomy. Methods From August 2013 to September 2015, after mastectomy with conservation of nipple and areola, twenty-three patients with stage 0,Ⅰ,Ⅱbreast cancer accepted immediate breast reconstruction with pedicled greater omentum and gel prosthesis. Results All the pedicled greater omentum was successfully inserted, and no prosthesis migration and rupture occurred 3 to 25 months after operations. The reconstructed breast was good in shape and with soft feeling. Nipple ischemia occurred in two patients with ductal carcinoma in situ, while skin necrosis was not found within 3 months. There was no recurrence of breast cancer during follow-up period. Conclusion Immediate breast reconstruction with pedicled greater omentum and gel prosthesis after mastectomy with conservation of nipple and areola is an effective and safe procedure.

目的：探讨麦默通微创旋切系统在临床诊断和治疗不可触及乳腺肿块中的应用。方法：收集我科自2014年9月～2015年9月麦默通手术患者的临床资料，总结其临床经验。结果：188例患者的乳腺肿块均被安全的切除，术后有13例患者出现血肿或硬结，有2例患者出现皮下瘀斑，术后随访均未出现肿瘤的残留与复发。结论：麦默通旋切系统在不可触及乳腺肿物的治疗中具有定位精准、创伤小、安全性高及术后美观等优点，值得临床广泛应用。

Objective:To explore the curative effects of infraclavicula lymph node dissection via pectoralis approach in the treatment of breast cancer. Method:86 cases were divided into the observation group(n=43) and the control group(n=43) according to the random number table.The control group was given conventional surgery with electric scalpel,while the observation group was given infraclavicula lymph node dissection via pectoralis approach.The two groups of patients were compared for operation time,volume of drainage,intraoperative blood loss and complications.Result:The observation group had significantly shorter operation time,more volume of drainage and less intraoperative blood loss than the control group,with statistical differences(P<0.05);and the incidence of complications in the observation group was significantly lower than the control group,with statistical difference(P<0.05).Conclusion:For patients with breast cancer,infraclavicula lymph node dissection via pectoralis approach combined with Auchincloss can thoroughly remove the lymph nodes with shorter operation time,more volume of drainage,less intraoperative blood loss and fewer complications.

目的 比较TAC与TC新辅助化疗方案治疗局部晚期乳腺癌的临床疗效和不良反应.方法 经病理等检查确诊局部晚期乳腺癌患者257例,随机分为TAC组(130例)及TC组(127例),分别给予TAC方案、TC方案化疗4个周期.TAC组129例、TC组124例化疗结束2周后行乳腺癌改良根治术.比较TAC组、TC组疗效和不良反应,治疗前、后TNM分期变化及术后并发症.结果 临床疗效比较TAC组优于TC组,其差异有统计学意义(P＜0.05)；TNM分期较新辅助化疗前降低(P＜0.05),不良反应比较差异无统计学意义(P＞0.05)；术后并发症比较差异无统计学意义(P＞0.05).结论 TAC及TC新辅助化疗方案对局部晚期乳腺癌均有效,TAC方案优于TC方案.

Objective: To compare the impacts between breast-conserving surgery and modified radical treatment on clinical efficacy and quality of life in patients with early breast cancer. Methods: The clinical data of 95 patients with early breast cancer undergoing breast-conserving therapy(breast-conserving group) between July 2004 and July 2007 were analyzed,and were compared with those of 95 matched patients over the same period undergoing modified radical mastectomy(modified radical group). Results: There were no significant differences between the two group in recurrence,metastasis and survival rate(all P0.05).The rate of aesthetically excellent breast shape in breast-conserving group and modified radical group was 93.7% and 0,respectively(P0.05).The quality-of-life score of the 5-year survival patients in breast-conserving group was significantly higher than that in modified radical group(83.66±3.70 vs.73.07±4.85)(P0.05). Conclusion: In early breast cancer patients,breast-conserving surgery can achieve the same therapeutic efficacy as modified radical mastectomy,and meanwhile significantly improve their quality of life.Strict observation of the indications,standardized surgical resection and individualized,comprehensive postoperative management are critical to success of breast-conserving treatment.