This study examined the effects of kolaviron and quercetin, individually and combined, on biochemical and histopathological changes in testosterone-induced benign prostatic hyperplasia (BPH) in male Wistar rats. Forty-two rats were divided into six groups, with BPH induced in all but the control group. Treatments included kolaviron, quercetin, their combination, and finasteride. BPH increased PSA, 5-α reductase, DHT, kidney and liver function markers, and altered lipid profiles. Treatments reduced these elevations and improved testosterone and HDL-c levels. Findings suggest that kolaviron and quercetin effectively ameliorate BPH, indicating their potential as affordable, non-invasive options for BPH management.

Oxidative stress plays a crucial role in the development of type 2 diabetes and the associated microvascular and cardiovascular complications. In the study, we have investigated the effects of Heinsia crinita (H. crinita) extracts on lipid peroxidation and oxidative stress responses using diabetic rats. Type 2 diabetes was induced with 10 % fructose/40 mg/kg body weight streptozotocin (STZ). H. crinita extract was administered at 200 and 400 mg/kg body weight twice daily for 21 days, in addition to metformin (MET: 500 mg/kg body weight) control. Molecular docking analysis was performed to determine the binding affinity of H. crinita extracts to the DNA binding domains of peroxisome proliferator-activated receptor (Ppar) and retinoid x receptor (Rxr) protein crystal structures, showing different binding affinities for putative active compounds from the plant. Fasting blood glucose (FBG), body and organ weight changes were determined showing that H. crinita extract induced an anti-hyperglycemic effect in the treated animals, with changes (either decrease or increase) in liver and kidney weights. A decrease in mRNA expression of peroxisome proliferator-activated receptors (ppar), sterol regulatory element-binding protein 1 (srebp-1c), liver x-receptor (lxr), retinoid x receptors (rxr), cytochrome p45041 (cyp4a1) and acyl-CoA oxidase (acox1) in diabetic animals were observed, compared to the control. A dose-specific decrease or increase in antioxidant enzymes (superoxide dismutase: SOD, catalase: CAT, reduced glutathione: GSH, glutathione peroxidase: GPx) transcripts and activity levels were also observed. We also observed exposure-specific decrease or increase of malondialdehyde (MDA) levels. Our data suggested that H. crinita extract possesses protective effects against diabetes-induced oxidative stress. These effects might be attributed to their binding and activation of nuclear receptors, indicating their cellular mode of action that is comparable to MET.

Objectives: Staple food to mobilize micronutrients as well as agricultural effects to improve food availability, affordability, and accessibility, in the utilization of food to address nutrient deficiencies and other nutritional challenges. This study will explore strategies and mechanisms for assessing the mineral composition of rice-based composite flour produced from blends of rice, soybean, coconut, water-yam, and sweet potato flours. Material and Methods: Different blends of the composite flours will be prepared as A, B, C, etc. The mineral composition of the rice-based composite flour and its cookies was determined by the spectrophotometric method (applicable to Na, Fe, Ca, K, and Mg). Results: The mineral contents of the composite flour samples analyzed in the present study ranged between 30.47 ± 1.41 and 82.00 ± 0.57, 0.24 ± 0.02 and 1.27 ± 0.07, 17.37 ± 0.46 and 87.97 ± 0.24, 104.27 ± 0.54 and 701.63 ± 1.32, and 12.17 ± 78.00 and 78.00 ± 0.67 mg/100 g for sodium (Sa), iron (Fe), calcium (Ca), potassium (K), and magnesium (Mg), respectively, while the mineral contents of the rice-based composite cookie samples analyzed ranged between 170.67 ± 8.45 and 383.33 ± 4.10, 1.07 ± 0.01 and 2.75 ± 0.02, 45.77 ± 0.78 and 64.73 ± 2.17, 113.97 ± 1.46 and 144.23 ± 0.52, and 27.77 ± 0.35 and 74.73 ± 1.73 mg/100 g for Na, Fe, Ca, K, and Mg, respectively. There was a significant difference ( P < 0.05) in the mineral composition of the samples. Conclusion: The finding of this study is a wake-up alert for policymakers to improve and increase availability, accessibility in the utilization of food to address nutrient deficiencies and other nutritional challenges in the country.

Benign prostatic hyperplasia is the most common male benign proliferative disease; characterized by overgrowth of prostatic tissue around the urethra constricting the urethral opening. The study investigated the effect of Anchomanes difformis ethanol root extract on electrolyte level of testosterone induced benign prostatic hyperplasis in Wistar rats. Forty two matured male Wistar rats weighing 150-180 g were divided into 6 groups of 7 animals each and acclimatized for 7 days. Prostate was induced with 5mg/kg of testosterone for 21 days. Two animals were sacrificed and blood collected for PSA assay. Groups 4, 5 and 6 were treated with 1000 mg/kg, 500mg/kg and 250 mg/kg of the extract respectively. The testosterone induced group 3 was treated with finesteride standard control and group 2 represented the BPH induced control group, while group 1 served as normal control. The administration of the extract lasted for 21 days. The results obtained showed a non significant (P>0.05) increase in Na+ (mmol/L) concentration at 1000mg/kg of plant extract Bph C group vs normal control, while the finesteride group had no effect on Na+ concentration treated with Bph C group. There was a significant (p<0.05) reduction in Cl concentration at 1000mg/kg vs 5mg/kg of testosterone and 5mg/kg and finesteride, with no significant (p>0.05) effect at 500 mg/kg Vs normal control. For K+ (mmol/L) concentration in plant treated groups, 1000 mg/kg increase significantly at 250 mg/kg. Conclusively, the results suggested, Anchomanes difformis root extract has the potential to maintain serum electrolytes in benign prostatic hyperplasia management induced by testosterone and diarrhea patients.

The proximate constituents and phytochemical compositions of Bambara groundnut (Vigna subterranean) and Pigeon pea (Cajanus cajan) underutilized in Calabar, Cross River States was investigated. The research will provide solutions to food availability and affordability to interlock their abundance in complex carbohydrates, plant-based proteins, unsaturated fatty acids and essential minerals. Bambara groundnut (Vigna subterranean) and pigeon pea (Cajanus cajan) obtained from markets in Anambra State and Ugep, Cross River State. The plant extracts were assessed for the existence of phytochemicals by using standard procedures. Phytochemical screening revealed that the plant species used in this research contained alkaloids, flavonoids, and polyphenols. Proximate analysis of the seeds revealed the moisture content of 9.70% for Bambara groundnut and 10.01% for Cajanus, crude fat of 7.50% for Bambara groundnut and 4.50% for Cajanus cajan, ash content of 3.21% for Bambara groundnut and 3.31% for Cajanus cajan, crude protein of 25.01% for Bambara groundnut and 23.06% for Cajanus cajan, and carbohydrates composition of 70.41% for Bambara groundnut and 71.04% for Cajanus cajan. The presence of phytochemicals such as flavonoids, alkaloids, and polyphenols explained the medicinal action of the plants encountered in their therapeutic usage to meet both domestic and commercial needs.

Background Diabetes is a serious metabolic disorder and many medicinal plants are used in traditional medicine to manage it. This study aimed to evaluate the therapeutic effects of Vernonia calvoana ( V. calvoana ) extract fractions on streptozotocin-induced diabetic rat models. In this study, we first investigated the binding affinity of ligands from extracts of V. calvoana crystal structure proteins using a molecular docking approach. Furthermore, the in silico predictions were validated by in vitro and in vivo biochemical evaluations to ascertain the efficacy of these extract fractions. The in vitro antioxidant activity of the fractions was evaluated using DPPH, FRAP, SOD, and LPx scavenging. For biological activity, extract fractions of V. calvoana and metformin (400 mg and 500 mg/kg body weight, respectively) were administered to diabetic rats for 21 days after induction and confirmation of diabetes. Results The radical scavenger activities of the fractions showed a good dose-dependent reaction activity. A significant reduction in hyperglycemia, hyperlipidemia, nephrotoxicity, and hepatotoxicity was observed in all experimental treated groups. Improved hematological and histopathological changes were also observed. Conclusion The In silico analyses revealed that all the compounds from extract fractions of V . calvoana have varying binding affinity for PFK and lipoprotein lipase, with some showing higher affinity than the standard drug, further validating the biological activity of the plant. The results of this study indicated that V. calvoana extracts might have potential value in treating complications arising from diabetes mellitus.

Objectives: Dareta village in Zamfara state of Nigeria is one of the villages plagued with the lead (Pb) poisoning crisis of 2010. Dareta and neighboring villages were remediated by simple excavation of topsoil rich in Pb, and burying same in landfills. This study was embarked to assess the physicochemical parameters of some sources of water supply and possible health impacts on humans. Material and Methods: The village was imaginarily mapped into three sites; Residential Homes (Site 1), Markets and Village Squares (Site 2), and Farms with Irrigation Facilities (Site 3). From the three sites, a total of 30 water samples (10 shallow wells, 10 boreholes, and 10 stream/surface water) were randomly collected, according to availability. The physical parameters (Temperature, Conductivity, and Total Dissolved Solids [TDS]) were assessed in situ using a conductivity/TDS meter (model 44600.00, HACH, USA), the assessment of pH was also carried out in situ using an electronic pH meter, (pH 210 micro-processor, Hanna Instrument, USA). The evaluation of heavy metals concentration (Pb, cadmium [Cd], chromium [Cr], and manganese) was done using an Atomic Absorption Spectrophotometer (modelAA-6800, Schemadzu, Japan). Results: In groundwater (boreholes and wells), the mean temperature and conductivity were higher than the World Health Organization (WHO) safety levels for “drinking water,” while those of TDS and pH were within the limits. In surface water (streams), the assessed physical parameters were not higher than the WHO safety limits, while the concentrations of Pb, Cd, and Cr were above the drinking water guidelines. The risk implication in the human populations consuming the water was determined using the estimated average daily intake (EADI) and the target hazard quotient (THQ). The EADI for Pb, Cd, and Cr for all the water sources in both adult and children consumer populations exceeded the reference dose by US-EPA. The THQ for Pb, Cd, and Cr in both adult and children consumer populations were >1. Conclusion: These findings portend toxicity and increased hazards for the human populations that source their drinking water from the borehole, well, and stream in this village.

Objectives: Sudan IV dye (S4D), has been reported to enhance the color of food products example palm oil, despite the health issues associated with these colorants. The potent antioxidant attribute of Curcuma longa facilitates its capacity to protect the kidney against deleterious attacks by free radicals. This study investigated the rejuvenating potentials of the extract of C. longa by S4D induced hepato-renal injury in rats. Material and Methods: Twenty-five female albino rats were distributed into five groups, as follows: Group 1 received distilled water and diet only, Group 2 received 10% S4D+100 mg C.L, Group 3 received 15% S4D +100 mg C.L, while Groups 4 and 5 received 200 mg and 250 mg C.L, respectively. After 3 weeks of administration of Curcuma treatment, animals were sacrificed; liver, kidney, and small intestine were collected for analyses. Aspartate and alanine aminotransferases, gamma-glutamyl transferase, glutathione-s-transferase, alkaline phosphatase, and lactate dehydrogenase, as well as some selected electrolytes were measured. Results: Results show that treatment with 200 mg and 250 mg/kg significantly (P < 0.05) rejuvenated these enzyme biomarkers while modulating the physiological levels of the serum electrolytes and kidney markers. Conclusion: C. longa exhibited a significant antioxidant potential that sustain the cells from the effect of S4D toxicity. Hence, the results indicate a possible ameliorative mechanism of the plant extract against S4D- induced hepato-renal injury. Therefore, the inclusion of C. longa into our daily diet might limit the effect of the hepato-renal injury.

Endocrine disruptors affect biological systems, some as neglected home producing toxicity (NHPT). We investigated the effect of morin on diethyl phthalate (DEP) and bisphenol S (BPS)-induced hematotoxicity and oxidative stress using a rat model. Rats were exposed to DEP (50 μg/kg) and BPS (200 μg/kg) and treated with morin (25 and 50 mg/kg) by oral gavage for twenty-one days. Afterwards, they were sacrificed. Morin treatment significantly (p < 0.05) protected red blood cells and plasma membrane architecture by abolishing the DEP+BPS-induced decrease in the activity of all the hematological indices. Co-treatment with morin remarkably (p < 0.05) reversed DEP+BPS-induced decreases in glutathione levels, CAT, GPx, and SOD activities in red blood cells, while attenuating DEP+BPS mediated increase in hemato-oxidative damage markers (MDA and NO levels). Collectively, the different acute doses of morin might avert DEP and BPS-mediated hematotoxicity dysfunctions via its antioxidant, free-radical scavenging effect.

This research work was aimed at investigating the effects of vitamin C, E and garlic interaction on tramadol induced toxicity in Wistar rats. Thirty five (35) rats were used for the study and rats were shared into five study groups namely; the positive control group fed with water and rats pellets, the negative control group was induced with tramdol only, the TmVC group in which 0.2mg of tramadol was induced and 0.2ml of vitamin C were administered, The TmVE group in which 0.2 mg of tramadol was induced and vitamin E was administered. The TmG group in which 0.2mg of tramadol was induced and garlic was administered. The body weight, organ weights and serum enzyme activities of the rats were monitored during the experimental period. The enzyme activity level of ALT remained constant in the positive control and negative control groups but began to rise in the TmVC group in which vitamin C was administered and kept rising in the TmVE group and the most significant increase came in the TmG group in which garlic was administered. AST was lower in all of the groups in which tramadol was administered compared to the positive control (group one) with the highest drop in the TmG group in which garlic was administered. ALP, however, showed significant decrease in all the groups except TmG when compared to the positive control at (P<0.05). High density lipoprotein was relatively low in negative control when compared to positive control at (P<0.05). High density lipoprotein was high in antioxidants treated groups compared to negative control at (P<0.05). The low density lipoprotein was high in negative control when compared to positive control. The antioxidant treated groups showed no significant decrease in low density lipoproteins when compared to negative control group.

Benign prostatic hyperplasia (BPH) is a common urological disorder reported among aging men. The pathophysiology of BPH is not clearly understood; however, its etiology is attributable to inflammation and/or oxidative stress. We investigated the effect of aqueous leaf extract of Ocimum gratissimum (OG) on BPH induced animal model. BPH was induced in male rats weighing (250-350 g) through exogenous administration of testosterone and estradiol. A total of 30 rats were divided into five groups. One group served as a normal control, and the other groups received subcutaneous injections of the hormones for 3 weeks to induce BPH. Groups 1 to 2 were treated orally with 50 and 100 mg kg –1 body weight (bw) of the extract respectively. While group 3 received finasteride at 0.1mg kg –1 , group 4 was left untreated, and group 5 served as normal control. After forty-five days of treatment with the extract, the animals were sacrificed. Blood was collected by cardiac puncture and the sera centrifuged and used for the determination of different biochemical indices. The liver and kidney were collected and homogenised and used for the assays of oxidative stress parameters. The activities of tissue superoxide dismutase (SOD) and catalase (CAT) in the extract treated rats were significantly increased when compared with the BPH control which had a significant reduction in the activities of these enzymes. Thiobarbituric acid reactive substance (TBARS) concentration decreased compared to BPH control group, while the concentration of reduced glutathione (GSH) in the extract treated group significantly (P<0.05) increased. Prostate tissue is prone to attack by reactive oxygen species (ROS) resulting in tissue damage. However, the outcome of this research revealed that Ocimum gratissimum possesses the potential to maintain tissue integrity and reverse oxidative stress caused by BPH.

Background: Benign prostate hyperplasia (BPH) is an age-related disease of unknown etiology, characterized by prostate enlargement. The effect of Prosopis africana (PA), Vernonia amydalina (VA) and Ocimum gratissmum (OG), plant extracts on haematological parameters of BPH animal model was investigated. Methods: BPH was induced in 45 male Wistar rats (250-350 g) by exogenous injection of testosterone and estradiol in staggered doses for 3 weeks. To confirm BPH induction, some animals were sacrificed; histological inspection of prostate gland and PSA was carried out. Forty BPH induced rats were divided into 8 groups. Group 1 and 2, 3 and 4, 5 and 6 were treated with 50 mg/kg bw and 100 mg/kg bw doses of PA, VA and OG extracts respectively. Group 7 received finasteride (0.1 mg/kg bw). Group 8 BPH control and five rats without induction constitute group 9, the normal control and both received distilled water. After 45 days, the rats were anaesthetised by a brief exposure to trichloromethane vapour and 5 ml of blood was collected from the rats through cardiac puncture and dispensed into well-labelled EDTA containers to avoid coagulation. All analyses were completed within 24 h of sample collection. Results: Results showed that induction of BPH caused a significant (P< 0.05) enlargement of prostate gland when compared to normal control. All extracts produced significant (P<0.05) reduction in the weight of the enlarged prostate when compared to the BPH control. There were significant (P ˂0.05) decline in RBC, PCV and Hb of BPH control when compared to the normal control and treated groups. In the treated groups the administration of the extracts and standard drug exhibited an increase in RBC, PCV and Hb concentration when compared with the BPH control. Also there was significant (P < 0.05) increase in the WBC, neutrophils, platelets, monocytes, lymphocytes and eosinophils levels in BPH control when compared to normal control and treated groups. In all treated groups there was significant decrease in WBC, neutrophils, platelets, monocytes, lymphocytes and eosinophils concentration levels when compared with the BPH control group. Conclusion: The result of this study indicates that the extracts have the potential to reverse the inflammation caused by BPH and also have the capacity to boost the numbers of red blood cells probably by inhibiting the hemolysis caused by inflammatory factors or by enhancing the production of red blood cell from the bone marrow.

Development of antimalarial drugs from medicinal plants continues to be a very appealing option. Morinda lucida, commonly known as ‘Oruwo’ or ‘Erewo’ by the Yoruba people of South Western Nigeria, is used in the treatment of malaria, fever, amongst other ailments. Therapeutic efficacy of the ethanolic leaf extract was evaluated in Plasmodium berghei infected mice. Percentage suppression of parasitaemia for the ethanolic leaf extract was 54.68, 60.84, 84.89 and 96% for doses of 100mg/kg, 200mg/kg, and 400mg/kg body weight of the extract and 5 mg/kg Leonart DS respectively. Effective dose which depends on the inhibition of parasitaemia was also observed in the curative test. The hepatoprotective activity of the ethanolic leaf extract was determined through liver enzyme tests (AST, ALT, and ALP) for 100, 200, and 400 mg/kg body weight of the extract. Morinda lucida ethanolic leaf extract possesses no lethality in mice at 400mg/kg body weight. It is concluded that the ethanolic leaf extract of Morinda lucida is potentially useful as antimalarial drug.

This study was conducted to investigate the effect of different processing methods of Bambara groundnut seed meal and its effects on tissue of broiler chickens. A total of eighty four day - old broiler chickens were weighed and randomly distributed into seven dietary treatments, three replicates of four birds per replicate. Each group was replaced with 40% of the processed Bambara groundnut (boiled, soaked, fermentation without decantation, fermentation with decantation, roasted and raw) respectively in place of soya bean meal. Soya bean meal based diet served as the control group, and the group treated raw Bambara groundnut meal serves as the negative control group. Liver, kidney and serum were studied by measuring the enzyme alkaline phosphatase (ALP), and conjugated and total bilirubin were considered. Body Weight Gain (BWG) was calculated as performance indices. Findings from this study indicated that ALP, conjugated and total bilirubin activities showed no significant differences (P<0.05) in the serum, liver and kidney when compared with the control. The study showed that broiler chickens serum and kidney functions were not affected with the replacement of 40% of the various processed Bambara groundnut meal with soya bean meal.

The effect of ethanol extract of Irvingia gabonensis seed on some renal and hepatic function biomarkers was studied in ten Wistar rats. The rats were randomly assigned into two groups of five rats each: Group 1 (Normal group) received normal rat feed and water; Group 2 (Treated group) received 250mg/kg body weight of ethanol extract of Irvingia gabonensis seed in addition to normal rat feed and water. Treatment lasted for twenty-one days and was done twice daily via oral gastric intubation. Blood samples were collected from each rat via cardiac puncture for analysis of hepatic and renal function biomarkers. Serum AST and total protein concentrations were significantly (p<0.05) increased and ALT and ALP concentrations significantly (p<0.05) decreased in the treated group compared with the normal group. Serum creatinine, Na + , and K concentrations were not significantly different between the two groups but Ca 2+ concentration increased significantly (p<0.05) in the treated group compared with the normal group. In conclusion, Irvingia gabonensis seed therefore has the potential to improve liver and kidney functions in Wistar rats and may serve as a source of calcium. Increased consumption of Irvingia gabonensis seed is encouraged.

Background: Benign prostate hyperplasia (BPH) is a common urological disorder in elderly men. We investigated the effect of aqueous extract of Vernonia amygdalina (VA) on BPH. Methods: BPH was induced in male rats weighing 250-350 g. Testosterone propionate (T) and estradiol valerate (E2) were used for the induction at a dose of 400 μG T and 80 μG E2 respectively. This was administered to the rats for three weeks subcutaneously in the inguinal region. A total of 30 rats were divided into five groups. One group was used as a control and the other groups received subcutaneous injections of the hormones for 3 weeks to induce BPH. Original Research Article Ugwu et al.; JOCAMR, 6(1): 1-12, 2018; Article no.JOCAMR.42506 2 Groups 1 and 2 were treated with different doses of VA extracts and group 3 received finasteride, all by gavages for forty-five days, while group 4 was left untreated, group 5 served as normal control. After forty-five days of treatment with VA extract, the rats were anaesthetised by short contact with trichloromethane vapour. Blood was collected by cardiac puncture and the sera cautiously centrifuged and used for the determination of different biochemical indices. The prostate was harvested and weighed. The harvested prostate were processed for paraffin embedding and stained with H and E. Results: Treatment with the extract and finasteride resulted to significant (P < 0.05) decrease in prostate-specific antigen (PSA), estradiol and prolactin, testosterone and protein content of the prostate when compared to BPH control. Prostate weight was significantly (P < 0.05) reduced in treated groups compared to BPH control. This was supported by the histological examination. Conclusion: Therefore, Vernonia amygdalina was effective in reducing PSA, prolactin, testosterone, estradiol and prostate weight induced BPH in a rat model, and may be useful for the clinical treatment of patients with BPH.

This study investigated the anti-diabetic effect of ethanolic bark extract of Khaya senegalensis in Alloxan-induced diabetic albino Wistar rats. Sixty (60) female albino rats were randomly placed into six (6) study groups of ten (10) animals designated as; non-diabetic control (NDC), diabetic control (DC), and diabetic extract treated groups (DSB1, DSB2, and DSB3) receiving varying extract concentration of 100mg/kg, 200mg/kg, and 400mg/kg body weight respectively. Animals were sacrificed after 28 days of treatment and blood was obtained for biochemical analysis. Body weight indices obtained from this study revealed a decrease (p<0.05) in body weight in the extract treated group when compared with non-diabetic control with an attendant increase (p<0.05) when compared with diabetic control. Phytochemical screening detected biologically important metabolites including phenolics and steroids while haematological parameters such as packed cell volume and haemoglobin decreased in the extract treated group when compared with the non-diabetic control but significantly increased (p<0.05) when compared with diabetic control. Lipid profile showed a significant decrease (p<0.05) in total cholesterol and low density lipoprotein (LDL) in the extract treated group with an attendant increase when compared to the diabetic control and non-diabetic control respectively. Serum liver enzymes; alkaline phosphatase (ALP) and alanine transaminase (ALT) were increased in the extract treated group when compared to the non-diabetic control but a significant decrease (p<0.05) was observed in comparison with the diabetic control. In conclusion, data generated from this study show that ethanolic bark extract of Khaya senegalensis possesses antihyperglycaemic activities in alloxan induced diabetic rats.

Aim: Malaria caused by Plasmodium falciparum is one of the killer diseases in Africa today and the uncontrollable spread of drug resistance and limited drugs with therapeutic efficacy makes it necessary to discover agents against this deadly parasite. Traditionally Anthocleista djalonensis root extract is used in the treatment of Malaria in many parts of Africa and has demonstrated to be a source of antiplasmodial agents. This study aims at identifying possible antiplasmodial agents from chromatographic root fractions of Anthocleista djalonensis of the Genatianceae family as well as to evaluate their cytotoxicity against HeLa cells. Place and Duration of Study: The study was undertaken in the Department of Organic Chemistry, Rhodes University, Grahamstown, South Africa. The duration period was between March and July 2016. Original Research Article Okoro et al.; IJBCRR, 24(2): 1-13, 2018; Article no.IJBCRR.36635 2 Methodology: The Anthocleista djalonensis roots were collected from Arochukwu, Abia State, Nigeria. The acetone extract was obtained from successive maceration of the methanol crude extract with hexane, ethyl acetate and acetone. The concentration (1-1000 μg/mL range) of the chromatographic fractions from acetone root extract of Anthocleista djalonensis were tested for antimalarial activity against Plasmodium falciparium (P. falciparum). Cytotoxicity against HeLa cells was also evaluated using Resazurin based assay. Results: The Five fractions obtained from the chromatographic fractionation of acetone extract labeled A1, A2, A3, A4, and A5 with percentage yield (13.02, 26.66, 24.70, 0.05 and 26.66% respectively) showed excellent anti-plasmodial activity. The anti-malarial bioassay test showed fractions A1, A2, A3, A4 and A5 with IC50 value of 0.0360 ± 0.0100, 8.1299 ± 2.0358, 46.2482 ± 1.2720, 0.0151 ± 0.0010, and 9.8013 ± 0.8171 μg/mL respectively. CC50 values of 44.2010 ± 8.6790, 50.0000 ± 5.6412, 71.6221 ± 2.9600, 36.7212 ± 5.8900 and 0.5132 ± 3.770 μg mL –1 were recorded for fractions A1, A2, A3, A4 and A5 respectively. Fractions were classified as marginally active (A3) showing SI of 1.540 ± 0.0091, partially active (A2 and A5) with SI 6.150 ± 0.0200 and 4.133 ± 0.015 and as active (A1, A4,) exhibiting SI of 1227.805 ± 8.210 and 2431.867 ± 1.589 respectively. A1 and A4 showed SI > 10 and IC50 < 10 ug/mL. Chloroquine, used as a reference anti-malarial drug, tested in parallel had an IC50 of 0.0125 ± 0.0001 μM and was comparable with A1 and A4. Conclusion: The chromatographic fractions from acetone root extract of Anthocleista djalonensis are potential sources for anti-malarial agents of lead compounds for the development of antiplasmodial drugs.