Resistance to acetolactate synthase (ALS) inhibitors have increased recently in South Brazil where the major weeds of flooded rice (barnyardgrass and weedy rice) have evolved resistance to imazapyr+imazapic. The aim of this research was to evaluate a growth medium for tissue regeneration of tillers in barnyardgrass, as well as an agar-based bioassays test (also from tillers) to detect susceptible and resistant biotypes of weedy rice and barnyardgrass to imazapyr+imazapic in vitro. Greenhouse experiments were conducted to detect ALS-resistant (R) and susceptible (S) weedy rice and barnyardgrass biotypes, and bioassays were carried out to evaluate an adequate growth medium for barnyardgrass tiller regeneration and determine the concentration of herbicide to distinguish R and S plants. The culture medium that provided a suitable barnyardgrass growth was MS 50% with the addition of benzylamino-purine. The tissue regeneration in vitro with the growth medium containing imazapyr+imazapic allowed to discriminate between R and S barnyardgrass and weedy rice plants. The concentration required for satisfactory control of susceptible barnyardgrass and weedy rice explants grown in vitro was 0.9 μM and 1.3 μM of imazapyr+imazapic herbicide, respectively. The bioassay in vitro using tiller regeneration provides an opportunity to predict effectively imazapyr+imazapic resistance in barnyardgrass and weedy rice.

Italian ryegrass (Lolium perenne spp. multiflorum Lam.) is a troublesome weed commonly found in winter cereals. Winter cover crops represent an alternative for weed suppression, reducing weed soil seed banks, and future infestations. The aim of this study was to evaluate the suppression of winter cover crops on reducing the soil seed bank and infestations of Italian ryegrass in fields managed under no-tillage system. Field experiment was carried out during three consecutive years, in a strip-plot design with four blocks, containing the winter cover crops. Wheat (Triticum aestivum), canola (Brassica napus), barley (Hordeum vulgare), common vetch (Vicia sativa), cereal rye (Secale cereale), feral radish (Raphanus sativus), and black oat (Avena strigosa) were sown in each strip (block) under no-tillage system and compared with weedy fallow. Seed bank was quantified at 0.0- to 0.05- and 0.05- to 0.10-m depths, whereas Italian ryegrass infestations were measured with a sample frame of 0.0625 m(2). Greater suppression and lower soil seed bank were reported in plots managed with cereal rye, common vetch, black oat, and feral radish, reducing up to 80% in the first year, and up to 96% after 3 yr of continuous management in comparison to weedy fallow. Italian ryegrass infestations increased four- to six-fold when managed with canola. High suppressor potential of Italian ryegrass occurred for cover crops with biomass dry accumulation greater than 8.7 tons ha(-1), especially to plants with a height upper to 1 m, plant characteristics essential to reduce strongly Italian ryegrass infestations and ensure weed-free fields throughout the time.

Introduction: A. hybridus interference in soybeans varies with population size and may be quantified by mathematical models that allow establishing the threshold levels. Objective: Determine the interference of A. hybridus escaped from an herbicide program and with multiple resistance to EPSPs and ALS-inhibiting herbicides on soybean yield and its economic threshold (ET). Methods: Two experiments were conducted in a randomized block design. The treatments consisted of populations of A. hybridus ranging from 0 to 41 plants m(-2) (2018/19) and 0 to 32 plants m(-2) (2019/20). The yield components and yield plant(-1) of soybean were evaluated. The rectangular hyperbola model was fitted to the soybean yield loss. ET was calculated according to yield potential, price paid for grains, control cost and weed control efficiency Results: The increase in populations of A. hybridus has decreased in the number of pods and grains(-1) of soybean in both periods. The parameter i was 4.47 and 8.32%, and the ET ranged from 0.16 to 1.21 plants m(-2) in 2018/19 and from 0.09 to 0.65 plants m(-2) in 2019/20. Conclusions: One A. hybridus m(-2) may reduce 6.4% in the grain yield of soybean. ET occurs within the range of 0.09 to 1.21 plants m(-2).

Italian ryegrass (Lolium multiflorum Lam.) is a weed broadly found in fields cultivated with wheat and barley crops. Seed inputs into the soil before harvesting winter crops increase seed bank, ensuring survival. This study evaluated the persistence of Italian ryegrass seeds subjected to burial depths over time. Experiments were carried out in the field for two years in randomized block experimental design with four repetitions. Dormant seeds harvested from mother-plants were put in nylon bags containing soil. Previously, seed viability was evaluated in a 1 % tetrazolium solution, and 50 viable seeds by repetition were buried at 0.5, 5.0, 10, and 20 cm depths. Seed persistence was evaluated by the percentage of deteriorated and remaining seeds, non-dormant seeds, abnormal seedlings, and viable and non-viable dormant seeds at 0, 30, 60, 90, 180, 360, 540, and 720 days after burial (DAB). Seed persistence increased at 10 and 20 cm of burial depth compared to seeds in the soil layers up to 5 cm. Moreover, burial depth at 10 and 20 cm showed lower percentage of deteriorated seeds (10 % lower) compared to 0.5 cm at 360 DAB. For non-dormant seeds, a higher percentage was found at 90 DAB, regardless of seed burial depth. Dormancy breakage occurred until 180 DAB, and more rapidly at 10 and 20 cm depths. At 540 DAB, more than 95 % of seeds were unviable, demonstrating short persistence of Italian ryegrass in the soil seed bank.

Barnyard grass (Echinochloa sp.) is one of the main rice weeds. Knowledge of its emergence can support management measures. The present study models barnyard grass emergence at different flooded rice sowing periods. Furthermore, the effectiveness of the hydrothermal time model in estimating barnyard grass emergence is analyzed. Field emergence was monitored three times a week during two sowing times (October and November) and two growing seasons (2017/18 and 2018/19), in rice sown and unsown areas. Data were converted to cumulative emergence based on total seedlings. Soil temperature and moisture data were used to determine hydrothermal time. The sowing in October led to a continuous emergence of barnyard grass, while the sowing in late November led to different emergence rates. The highest emergence rates of barnyard grass occur in the first sowing time. The hydrothermal time model is adequate to estimate barnyard grass emergence in both sowing times.

Italian ryegrass (Lolium multiflorum Lam.) is a grass weed found in winter cereals, with several glyphosate-resistant diploid populations. Furthermore, the use of Italian ryegrass tetraploid genotypes creates an opportunity to increase the forage demand in replacement of diploid populations. The aim of this research was to evaluate the crossing capacity between glyphosate-resistant diploid and tetraploid Italian ryegrass to transfer glyphosate resistance to the next generation. Reciprocal crosses were performed using the susceptible tetraploid cultivar INIA Titan and resistant diploid (SVA04 biotype) plants to obtain the F1 progeny. Germination testing, survival percentage, inheritance of glyphosate-resistance, and ploidy level were evaluated in F1 progeny. Crossing of susceptible tetraploid plants male and glyphosat resistant diploid female affected the germination and exhibited 4.5% of triploid plants. F1 progeny from the glyphosate-resistant diploid male and susceptible tetraploid female did not show germination problems, but 46% of the plants died during the initial seedling stage. For this progeny, 10 and 25% of the seedlings were composed of triploid and pentaploid individuals, respectively. Crossesbetween susceptible tetraploid female and resistant diploid male didnot transfer the glyphosate-resistantfor the plants. Susceptible tetraploid male crossing with diploid resistant female had a reduction of 47% the glyphosate-resistant plants compared to the homozygous glyphosate-resistantprogeny. Overall, tetraploid Italian ryegrass can be a safe tool to reduce and manage the increase of glyphosate-resistant diploid ryegrass in field conditions.

No monocultivo de arroz, as espécies daninhas mais adaptadas a este sistema de produção aparecem em maior incidência na área. A rotação de culturas pode ser uma alternativa para auxiliar na redução do banco de sementes de plantas daninhas. Nesse sentido o objetivo do trabalho foi avaliar a dinâmica do banco de sementes de capim-arroz (Echinochloa spp.) e papuã (Urochloa plantaginea) em terras baixas, em combinações de rotação de culturas e do manejo de solo. Foram utilizadas as culturas de arroz, soja e sorgo, sob o manejo de preparo do solo convencional e sistema de semeadura direta, cultivado em faixas. Foi avaliado o banco de sementes de capim-arroz e papuã após dois anos de cultivo, na camada de 0-5 e 5-10 cm de profundidade. A densidade do banco de sementes de capimarroz e papuã foi superior no monocultivo de sorgo em relação ao cultivo de arroz. Não houve diferença do banco de sementes de plantas daninhas entre diferentes camadas de solo, exceto para o papuã no monocultivo de sorgo. O monocultivo de sorgo, sem aplicação de herbicidas em pós-emergência, em terras baixas incrementa o banco de sementes de capim-arroz e papuã.

ABSTRACT: The intergenotypic competition of tetraploid ryegrass with natural diploid population may be a tool to reduce the frequency of resistant individuals in an area. This study aimed to identify and compare the phenological development, fitness cost, and competitive ability between diploid and tetraploid ryegrass genotypes. Genotypes were grown in pots, and the morphological variables of genotype growth were assessed every 15 days up to 120 days after emergence to evaluate the fitness cost. Phenological development and seed yield components were measured in a single time together with the fitness cost. Competitive ability was determined in a replacement-series experiment with proportions of tetraploid and diploid ryegrass, in which the number of tillers, plant height, leaf area, and shoot dry weight were assessed at 50 days after emergence. The results of fitness cost showed that the number of tillers, leaf area, root dry weight, and the number of seeds were higher for tetraploid ryegrass, which presented a higher competitive ability than the diploid genotype regardless of the tested proportions. Tetraploid ryegrass may be useful for reducing the frequency of herbicide-resistant diploid ryegrass because of its higher competitive potential.

ABSTRACT: The commercialization of ryegrass seeds with the presence of resistant biotypes is a serious issue that increses the dispersion of resistance to new areas without this problem. Therefore, this study aimed to develop a quick test to detect susceptible and resistant seeds to the glyphosate herbicide in order to identify contaminated seed lots with glyphosate resistance. Three experiments were carried out, one in greenhouse and the other two in the seed laboratory. First, the resistance factor (RF) was determined by a dose-response curve experiment in biotypes suspected to be resistant and susceptible to glyphosate. Then, the germination test was conducted with the selected biotypes under increasing glyphosate rates (0, 3.5, 6.9, 13.9, 27.8, 55.5, 111, 222, 445, 890, and 1,780 mg a.e. L-1), as the second experiment. The third experiment was made to verify the methodological efficiency of the germination test with glyphosate to identify different contamination ratios (0, 4, 12, 36, and 100%) of the resistant biotype in the seed lot. The different levels of susceptibility of the biotypes to glyphosate were confirmed by the RF of 154.7 based on C50. Germination of the susceptible biotype was inhibited more than 99% by the rate of 127 mg a.e. L-1, while the resistant biotype was a little affected. The germination test with 127 mg a.e. L-1 of glyphosate showed contaminations of 2, 5, 19, 39, and 86% in lots with 0, 4, 12, 36, and 100% of contaminated seeds, respectively. This methodology can detect glyphosate susceptible and resistant seeds and identify contaminated seed lots with resistant glyphosate biotypes.

ABSTRACT: Ryegrass is an important weed in wheat cultivations due to the evolution of resistance to different mechanisms of action. This study aimed to compare the phenological development, fitness cost, and competitive ability between ryegrass biotypes susceptible and with multiple resistance to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs) and acetolactate synthase (ALS) inhibiting herbicides. Fitness cost and phenological development were evaluated from biotypes grown in pots, and morphological growth variables were determined up to 140 days after emergence. Phenological development was evaluated with a fitness cost. Competitive ability was determined in a replacement-series experiment with proportions of resistant and susceptible ryegrass. The tested proportions were 100:0 (pure resistant ryegrass stand), 75:25, 50:50, 25:75, and 0:100% (pure susceptible ryegrass stand), where the number of tillers, height, leaf area, and shoot dry matter were evaluated at 50 days after emergence. The longer tillering period of resistant ryegrass was the main factor involved in the difference in phenological development between biotypes. The resistant biotype was superior to the susceptible regarding height, shoot dry matter, and absolute growth rate, while the susceptible biotype had a higher number of tillers and leaf area ratio. Thus, the resistant biotype had no fitness cost. For competitive ability, no difference was observed between biotypes in the different tested proportions when they occupied the same ecological niche.

O uso intensivo e repetido do método químico de controle na cultura do trigo selecionou populações de nabo (Raphanus raphanistrum e R. sativus) e azevém (Lolium multiflorum) resistentes a alguns herbicidas, em especial aos inibidores das enzimas ALS, ACCase e EPSPs. Para manejar as populações de plantas daninhas resistentes, é importante rotacionar os mecanismos de ação de herbicidas, tanto na dessecação como nas aplicações realizadas após a implantação da cultura. Assim, objetivou-se com este trabalho avaliar a eficácia do herbicida clomazone no controle de azevém e nabo, associado ou não ao protetor dietholate, na cultura do trigo. Os experimentos foram conduzidos em delineamento inteiramente casualizado (DIC) com quatro repetições. No ensaio 1 foram testadas doses de dietholate (0, 120, 240, 360 e 480 g para 100 kg de sementes de trigo cultivar TBIO Itaipu) e de clomazone (0, 198, 396 e 594 g ha-1) aplicado em pré-emergência. No ensaio 2, instalado de modo simultâneo ao ensaio 1, os tratamentos foram constituídos pela testemunha infestada, testemunha capinada, clomazone + dietholate (396 g ha-1 + 240 g para 100 kg de semente) – aplicado em pré-emergência, iodosulfuron-methyl (5 g ha-1) e pyroxsulam (18 g ha-1) – aplicados em pós-emergência. O tratamento das sementes de trigo com dietholate não interferiu no controle de azevém e nabo pelo clomazone aplicado em pré-emergência. O clomazone, mesmo aplicado em baixas doses, apresentou controle de azevém acima de 80%, podendo ser utilizado para o controle de biótipos resistentes aos herbicidas inibidores da EPSPs, ALS e ACCase. O iodosulfuron-methyl e o pyroxsulam apresentaram excelente controle de nabo e satisfatório de azevém.

A liberação comercial da soja Roundup Ready® favoreceu o manejo de plantas daninhas e possibilitou o cultivo da soja RR® e a aplicação do glyphosate em pós-emergência da cultura. No entanto, o uso intensivo deste herbicida selecionou biótipos de buva resistentes e dificultou o controle. Muitos trabalhos avaliam a eficiência técnica de herbicidas, porém, na maioria das vezes, o retorno econômico não é analisado na tomada de decisão sobre a escolha dos herbicidas a serem aplicados. O objetivo do trabalho foi avaliar a eficiência técnica e o retorno econômico da aplicação de associações e doses de herbicidas no manejo de buva resistente ao glyphosate em pré-emergência da soja. O experimento foi conduzido à campo no delineamento de blocos ao acaso com quatro repetições. Os tratamentos consistiram de associações entre glyphosate, 2,4-D, saflufenacil, diclosulam, chlorimuron-ethyl e flumioxazin. As variáveis analisadas foram controle de buva e fitotoxicidade na soja aos 7, 14, 21 e 35 dias após a aplicação e o rendimento de grãos da soja. O retorno econômico dos tratamentos foi calculado em função do custo dos herbicidas e do rendimento de grãos da soja. Os resultados de controle e rendimento demonstraram que a associação de glyphosate, chlorimuron-ethyl, flumioxazin e 2,4-D e, glyphosate, chlorimuron-ethyl, 2,4-D e saflufenacil apresentaram as melhores respostas de eficiência técnica e, a aplicação do glyphosate, chlorimuron-ethyl e 2,4-D apresentou o melhor retorno econômico. Entretanto, o aumento nas doses de chlorimuron-ethyl e sua associação com flumioxazin não melhorou o controle da buva.

As principais plantas daninhas encontradas na cultura do trigo são o nabo e o azevém, e seu controle em pós-emergência é baseado em herbicidas inibidores da enzima acetolactato sintase (ALS) e acetil coenzima A carboxilase (ACCase) que, em função da resistência, limita as opções de controle. Associações de herbicidas têm sido utilizadas em pós-emergência do trigo, porém, seus efeitos na cultura são pouco conhecidos. O objetivo deste trabalho foi avaliar a seletividade de associações de herbicidas e doses aplicados em pós-emergência do trigo. Foram realizados três experimentos a campo em sistema de semeadura direta, no delineamento de blocos ao acaso com três repetições. Os experimentos foram conduzidos nos municípios de Santa Bárbara do Sul-RS, Cruz Alta-RS e Tupanciretã-RS. Foram estudadas doses e associações entre os herbicidas 2,4-D, metsulfuron-methyl, iodosulfuron, piroxsulam e saflufenacil aplicados no perfilhamento do trigo. As variáveis avaliadas foram fitotoxicidade aos sete, 14, 21 e 35 dias após a aplicação (DAA), rendimento de grãos (kg ha-1) e peso hectolitro dos grãos de trigo (PH), sendo os dados dos três experimentos analisados de forma conjunta. Aos 35 DAA as maiores fitotoxicidades foram observadas para metsulfuron-methyl na dose de 9 g i.a. ha-1 e para saflufenacil associado ao metsulfuron-methyl. Os resultados de rendimento de grãos demonstraram que a associação entre 2,4-D e metsulfuron-methyl, saflufenacil isolado ou associado à metsulfuron-methyl e o iodosulfuron-methyl foram seletivos para o trigo. O aumento da dose de metsulfuron-methyl (6 e 9 g i.a. ha-1) e a dose de registro de piroxsulam causaram redução significativa na rendimento de grãos de grãos de trigo na média dos três experimentos. O PH dos grãos de trigo não foi influenciado pelos herbicidas estudados.

O nabo (Raphanus raphanistrum) é uma planta daninha que interfere na produtividade final do trigo. O uso repetido de herbicidas inibidores da enzima ALS favoreceram a seleção de biótipos resistentes, tornando necessário o uso de herbicidas alternativos. O objetivo do trabalho foi avaliar o controle de nabo e seletividade ao trigo sob diferentes doses de saflufenacil e bentazona, e outros herbicidas de uso comum nesta cultura. O estudo foi realizado em casa de vegetação em delineamento inteiramente casualizado com quatro repetições. Os tratamentos de saflufenacil (0; 8,5; 17; 35 e 70 g ha-1), bentazona (0; 180; 360; 720 e 1440 g ha-1), metsulfurom metílico (3,6 g ha-1) e 2,4-D (670 g e.a. ha-1), além da testemunha capinada. As variáveis analisadas foram controle de nabo e fitotoxicidade ao trigo aos 8 e 14 DAA. Os resultados evidenciaram que o controle de nabo foi superior a 90% aos 14 DAA para doses superiores a 17 e 360 g ha-1 de saflufenacil e bentazona, respectivamente. Ao comparar com os tratamentos adicionais, observou-se que ambos os herbicidas podem substituir o metsulfurom-metilico e o 2,4-D para controle de nabo.

Na atualidade torna-se importante testar novas alternativas de moléculas herbicidas, mesmo que não sejam registradas para o controle de plantas daninhas infestantes do trigo, para avaliar a fitotoxicidade à cultura e a possibilidade de manejar plantas daninhas resistentes aos produtos utilizados para esse fim. Diante disso, um experimento foi realizado para avaliar a fitotoxicidade do herbicida clomazone associado ou não ao safener dietholate aplicados à cultura do trigo. O experimento foi realizado em delineamento inteiramente casualizado, arranjado em esquema fatorial (5 x 4) + 2 + 2, com quatro repetições. No fator A alocou-se as doses de dietholate (0, 120, 240, 360 e 480 g para 100 kg de semente) e o no B as doses de clomazone (0, 198, 396 e 594 g ha-1). Os tratamentos adicionais foram constituídos pelas testemunhas infestadas e sem herbicida, e a aplicação em pós-emergência de iodosulfuron-methyl (5 g ha-1) e pyroxsulam (18 g ha-1). O incremento das doses de clomazone ocasionou o aumento da fitotoxicidade ao trigo, associado ou não ao protetor. O dietholate não apresentou capacidade de proteger o trigo dos efeitos fitotóxicos do herbicida clomazone. Não é recomendado o uso do clomazone associado ao diethole para o controle de plantas daninhas infestantes do trigo. O pyroxsulam foi o herbicida que causou menores injúrias ao trigo.