Symbiotic bacteria have a significant impact on the formation of defensive mechanisms against fungal pathogens and insecticides. The microbiome of the mosquito Aedes aegypti has been well studied; however, there are no data on the influence of insecticides and pathogenic fungi on its structure. The fungus Metarhizium robertsii and a neurotoxic insecticide (avermectin complex) interact synergistically, and the colonization of larvae with hyphal bodies is observed after fungal and combined (conidia + avermectins) treatments. The changes in the bacterial communities (16S rRNA) of Ae. aegypti larvae under the influence of fungal infection, avermectin toxicosis, and their combination were studied. In addition, we studied the interactions between the fungus and the predominant cultivable bacteria in vitro and in vivo after the coinfection of the larvae. Avermectins increased the total bacterial load and diversity. The fungus decreased the diversity and insignificantly increased the bacterial load. Importantly, avermectins reduced the relative abundance of Microbacterium (Actinobacteria), which exhibited a strong antagonistic effect towards the fungus in in vitro and in vivo assays. The avermectin treatment led to an increased abundance of Chryseobacterium (Flavobacteria), which exerted a neutral effect on mycosis development. In addition, avermectin treatment led to an elevation of some subdominant bacteria (Pseudomonas) that interacted synergistically with the fungus. We suggest that avermectins change the bacterial community to favor the development of fungal infection.

Two methods of estimating the emergence and transfer of biomass of blood-sucking mosquitoes (Diptera and Culicidae) are compared. The phonological and quantitative data obtained by the hydrobiological method (using an aquatic net and a tray) and using cone-shaped traps are different. Nevertheless, the general characteristics of the studied processes are similar. Results of the hydrobiological method showed that the total value of the annual transfer of biomass by Culicidae accounts for 3.157 g wet weight m-2 per year; that of cone-shaped traps is 3.457 g wet weight m-2 per year.

The population structure of bloodsucking mosquito larvae in temporary and constant water bodies of the northern Kulunda steppe was investigated. The seasonal dynamics of the population density, the number of species in different types of reservoirs, and average density of each species are given. The productivity of water reservoirs in relation to mosquitoes of the family Culicidae is analyzed. The basic factors affecting the distribution of larvae of dominant species between different water bodies were revealed. The degree of correlation between the structure of bloodsucking mosquito larva assemblages and the type of the reservoir was established.

The data are given on the composition of amphibiotic insects collected in different years in the water bodies in the suburban zone of Novosibirsk. Aspecial attention is paid to the larvae of mosquitoes and midges of the families Culicidae and Chaoboridae. It is suggested that the larvae of Chaoborides are the potential biological regulators of the populations of mosquitoes.

The data are given on malaria incidence of population of the Novosibirsk oblast from 1924 till present time. The paper examines the modern state of the populations of its vector, Anopheles , in the region. Attention is paid to the possibility of resumption of local transmission of this disease on the territory of the oblast.

Keys to 10 Palaearctic species of the dragonfly genus Aeschna are given. For adults of the two morphologically similar species Ae. juncea (Linnaeus, 1758) and Ae. subarctica Walker, 1908, new distinguishing characters are given. These are the shape of the genital plate and position of the anal appendages relative to the horizontal plane of the female body, and the shape of the processes of the anterior hooks of the male genitalia. Additionally, keys to the larvae based on characters of larvae reared in the laboratory are given. These keys allow species-level identification for early and middle larval instars.