Objective The objective of this study was to evaluate the intake and nutrient digestibility, nitrogen balance and ruminal ammonia nitrogen in lambs of diets containing different levels of residual frying oil. Methods Levels of 0, 20, 40, 60, and 80 g/kg dry matter (DM) base of residual frying oil in the diets of lambs were evaluated. Five castrated lambs with initial body weights of 36.8±3.3 kg, distributed in a Latin square (5×5) design, were used. Results There was a decreasing linear effect on the intake of DM, organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), total carbohydrates (TCH), and nonfibrous carbohydrates (NFC). There was an increased linear intake of ether extract (EE). The apparent digestibility of DM, OM, CP, NDF, TCH, and NFC, as well as urine nitrogen excretion, nitrogen balance and ruminal parameters, were not influenced by different levels of residual frying oil in the diet. EE digestibility presented a crescent linear effect. Conclusion It can be concluded that the addition of residual frying oil to the diets of sheep can affect nutrient intake without affecting the digestibility of most nutrients (with the exception of EE), nitrogen balance and ruminal ammonia nitrogen concentration.

The objective of this study was to evaluate the replacement of soybean meal by soybean cake on carcass characteristics and meat quality of Santa Ines lambs. Twenty-nine intact males were used, weighing 23.63 ± 2.44 kg, at 80 days of age, distributed randomly, submitted to five diets, with the diets differing in the levels of substitution of soybean meal by soybean cake (0, 25, 50, 75 and 100%). Each diet was provided to six animals, except the 25% diet, in which five animals were used. Carcass characteristics were evaluated, and the Longissimus dorsi was removed for meat quality analysis. The different levels of soybean cake did not affect carcass characteristics. Regarding the characteristics of meat, there was a quadratic effect for the shear force and negative linear effect on flavor intensity, but no significant differences on the other parameters were observed. Replacement of soybean meal for soybean cake can be made without affecting most of the carcass and meat characteristics, and high levels of soybean cake in the diet can improve the meat sensorial quality of lambs receiving concentrated diets. Keywords: biodiesel; morphometry; Santa Ines; sensorial analysis; shear force.

Resumo Objetivou-se avaliar a substituição do farelo de soja pela torta de soja sobre as características de carcaça e qualidade da carne de cordeiros Santa Inês. Foram utilizados 29 machos inteiros, com 23,63 ± 2,44 kg de peso vivo, 80 dias de idade, distribuídos de maneira inteiramente casualizada, submetidos a cinco dietas, sendo que as dietas variaram nos teores de substituição do farelo de soja pela torta de soja de 0, 25, 50, 75 e 100%. Para cada teor de substituição foram utilizados seis animais, mas no teor de 25%, cinco animais. Foram realizadas avaliações das carcaças e retirou-se o Longissimus dorsi para análises da qualidade da carne. As características da carcaça não foram afetadas pelos diferentes teores de torta de soja. Observou-se efeito quadrático para a força de cisalhamento e efeito linear decrescente para a intensidade de odor, mas não foram observadas diferenças nos demais parâmetros. A substituição do farelo de soja pela torta de soja pode ser realizada sem prejuízo à maioria das características da carcaça e de carne e altos teores de torta de soja na dieta podem resultar em benefícios às qualidades sensoriais da carne de cordeiros recebendo dietas concentradas.

ResumoO objetivo deste trabalho foi avaliar o efeito da substituição do farelo de soja pela torta de soja em dietas para cordeiros Santa Inês confinados, avaliando o desempenho, consumo de nutrientes, comportamento ingestivo e medidas biométricas. Foram utilizados 30 cordeiros machos não castrados, com 23,63 ± 2,44kg de peso corporal, distribuídos de maneira inteiramente casualizada em cinco tratamentos, sendo assim 6 repetições por tratamento, os quais foram os teores de substituição do farelo de soja pela torta de soja de 0; 25; 50; 75 e 100%. O comportamento ingestivo foi avaliado através de observações pontuais a cada 10 minutos durante 24 horas. Não foi observado efeito significativo sobre os parâmetros de desempenho, consumo de matéria seca e de comportamento ingestivo. O ganho de peso médio diário foi de 312,73g, o consumo médio de matéria seca foi de 1,44kg/dia. A inclusão da torta de soja levou a um aumento linear no consumo de extrato etéreo em gramas/dia, em porcentagem do peso corporal e em porcentagem do peso metabólico. Foi observado efeito cúbico sobre parâmetros de consumo de fibra em detergente ácido. Os tempos de ruminação, alimentação e ócio foram de 425,85; 194,50 e 789,29min/dia, respectivamente. Em relação às medidas biométricas, não foi encontrado efeito da substituição do farelo de soja pela torta de soja. A torta de soja pode ser utilizada na substituição do farelo de soja, devendo-se atentar ao teor de extrato etéreo residual presente neste coproduto da indústria do biodiesel.

The objectives of this study were to analyze the productive performance of lambs, out of ewes of different genetic groups and submitted to different feeding managements before and during the mating season, from birth until weaning. One hundred and twenty-six lambs were used, from the genetic groups ½ Corriedale + ½ Texel (11), Hampshire Down (73), Ile de France (26) and Suffolk (16), born in two consecutive years. The ewes were submitted to three feeding managements: T1 – without supplementation, T2 - supplemented with 600 g/day of corn grain, and T3 - supplemented with 600 g/ day of a ration containing 75% of corn and 25% of soybean meal. Body weight and weight gain were analysed individually and by litter size. Flushing did not influence weight and weight gain of lambs. Twin lambs, in the individual analysis, showed lower body weight and weight gain compared to single lambs, but in the analysis by litter size, the sum of the twin lambs showed greater body weight and weight gain compared to single lambs. Differences in body weight and weight gain were observed among the genetic groups and between the sexes, where males were heavier and presented greater weight gain. Twin lambs showed higher mortality than single lambs. Although flushing can provide greater number of lambs, a considerable profit was not verified. It is concluded that flushing do not influence in the performance of lambs, and that twin lambs show higher mortality than single ones, and flushing is not profitable.

This study aimed to determine the carbohydrate fractions and estimate ruminal fermentation kinetics parameters of silages Brachiaria brizantha cv. Marandu with the inclusion of two microbial additives: Additive LPPP Pasture (Lactobacillus plantarum and Pediococcus pentosaceus) and additive BSLPPA Pasture (Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici). The experimental design was completely randomized with three different silages and three replications per silage. The silos were opened after 34 days of fermentation and then sent for laboratory analysis. For the fractionation of carbohydrates were determined total carbohydrates (TC), non-fiber carbohydrates (NFC), fractions A + B1 (fractions of fast and average ruminal degradation), B2 fraction (fraction slowly degraded in the rumen) and fraction C (carbohydrates no digestible in the rumen). The gas production data were fitted to a logistic model bicompartimental. There was no difference in the values of the variables of fiber degradation (FC) kinetic and no-fiber carbohydrates (NFC) of the silages. However, silages Brachiaria brizantha cv. Marandu with use of additives LPPP and BSLPPA had higher fractions of digestible carbohydrates, reducing total carbohydrates and low indigestible fiber fraction of carbohydrates. Thus, the two microbial additives improved the nutritional value of silage Marandu-grass.

The objective of this study was to evaluate carcass characteristics and meat quality of lambs fed sunflower cake to replace the protein of cottonseed meal. The experiment was conducted at the sheep sector at the Farm School, State University of Londrina. Thirty Santa Inês lambs, intact males, with age and weight at the beginning of the experiment of 80 days and 21.45 ± 2.16 kg, respectively, were used. It was used a completely randomized experimental design. The animals were divided into 5 treatments, with 6 replicates per treatment, according to the amount of protein of cottonseed meal replaced by protein of the sunflower cake (0, 20, 40, 60 and 80% of replacement). Carcasses were evaluated and the Longissimus dorsi was removed for analyzes of shear force, color, pH, marbling, water loss by pressure, sensory analysis, chemical analyses and lipid oxidation. Hot and cold carcass yields showed quadratic distributions, where the best values were found for treatments with low inclusion of sunflower cake. Carcass measurements, as well as cut yields were not affected by the content of sunflower cake. Regarding the parameters of lamb meat, only water losses during cooking and shear force were affected, being quadratic and linear, respectively. The meat was more tender in animals fed higher amounts of sunflower cake. The tasters of the sensory panel did not identify differences between treatments, and classified the meat with moderate odor intensity, good tenderness, low to moderate juiciness and medium acceptability. The replacement of cottonseed meal protein by sunflower cake protein in the levels used, did not affect the majority of the carcass characteristics and the meat quality.

This study aimed to assess levels of nitrogen additive Silogen® pasto on ruminal in vitro degradability gas production in Brachiaria decumbens silages. The nitrogen additive contained bacterial strains (Bacillus subtilis, Lactobacillus curvatus, Lactobacillus plantarum and Pediococcus acidilactici) and minimum nitrogen to 360 g/kg. The levels were: 0.0; 1.0; 1.5; 2.0 and 2.5%. In fractionation were calculated total carbohydrates (TC), non-fiber carbohydrates (NFC), soluble and rapidly degradable (A + B1), potentially degradable (B2) and degradable (C). The kinetic parameters of FC and NFC were estimated using the technique of in vitro gas production. Data were subjected to analysis of variance at 5%. The level additive did not influence the TC and fraction A + B1. The levels of nitrogen additive influenced the NFC, fractions B2 and C. For NFC content 2.0% showed the lowest (22.0 %). The lower levels of additive nitrogen (0, 1.0 and 1.5 % ) had higher fractions of B2 (average 40.2 %) and lower values for the fraction C (average 20.0 % ). There was no difference in the volume of gas CNF (average 86.73 mL) and final volume of gas produced (average 195.79 mL). Was no influence of nitrogen additives for the time of colonization, the lowest time of 3.89 h to 1.0%. The volume of gas of FC was influenced by levels of nitrogen additives with higher values to 0 % from 1.0 %, and 114.74 and 115.09 mL, respectively. Degradation rates of FC and FNC were also affected by the concentrations of nitrogenous additives, which presented higher rates to the levels of 2.0 and 2.5%. It follows that the lower levels of additives to 1.5 % promoted the reduction of the C fraction and increased B2, and greater production of gas volume of the fiber in these silages, showing better nutritional value in these silages.

Chemical analysis of carbohydrates and nitrogen fractions, as well as, determination their carbohydrates digestion rates in soyben meal (SM), crambe meal (CM), radish meal (RM), wet brewery residue (WBR) and dehydrated silkworm chrysalis (SCD) were accomplished. The kinetics parameters of non-fibrous carbohydrates (NFC) and B2 fraction were estimated using cumulative gas production technique. Among the foods studied there was considerable variation in chemical composition. The crambe meal was the only food that did not present synchronism between carbohydrate and nitrogen fractions. In this food there was predominance of A+B1 carbohydrates fractions and B1+B2 nitrogen compounds fraction, and for the other predominated B2 carbohydrate fraction and B1+ B2 nitrogen compounds fraction. There were differences among the digestive kinetic parameters for all foods. The greater participation in gas production due to non-fibrous carbohydrates was found in the crambe meal and oilseed radish meal. The fermentation of fibrous carbohydrates provided higher gas volume in the wet brewery residue and in the soybean meal, however, the soybean meal was food with higher total gas volume. Non fibrous carbohydrates degradation rates of wet brewery residue and dehydrated silkworm chrysalis were far below the limits of degradation of this fraction. Due to the parameters obtained by the cumulative gas production, the soybean meal was the best food, however, all others have potential for use in animal nutrition. The cumulative gas production technique allows the estimative of degradation rates and provides further information about the ruminal fermentation kinetics of foods.

The effects of ionophores (monensin) and probiotic (Saccharomyces cerevisiae + selenium + chromium) in diets with 80% forage were evaluated on the digestibility of nutrients. Three buffaloes, Murrah (Bubalus bubalis) and three cattle, Holstein (Bos taurus), with an average weight of 520 ± 30 kg and 480 ± 182 kg, respectively, with rumen cannula, over experimental design with two 3 x 3 Latin squares in a 3 x 2 factorial arrangement, with the absence or presence of additives: ionophore or probiotic and two species, were used. The internal flow indicator of fecal dry matter (DM) was the acid insoluble ash. DM, crude protein (CP) and neutral detergent fiber (NDF) ruminal degradability of Tifton 85 hay was conducted for cattle and buffaloes. A diet containing probiotics had higher dry matter and organic matter digestibility in buffalo and cattle, indicating a good performance in bulky diets. The potential and effective dry matter degradability in diet with probiotic in buffaloes, were smaller than diet with ionophore, suggesting that there was a better digestion of nutrients in the intestine of these animals. The potential and effective degradability of neutral detergent fiber and crude protein in the diet containing ionophores were superior than diet containing probiotic. Buffaloes showed higher capacity of dry matter and fiber digestion than cattle.

Propolis presents many biological properties, including antibacterial activities, and has been proposed as an additive in ruminant nutrition. Twenty bacterial strains, previously isolated from enrichments of Brazilian cow rumen contents in the presence of different propolis extracts (LLOS), were characterized using phenotyping and 16S rRNA identification. Seven strains were assigned to Streptococcus sp., most likely S. bovis, and were all degrading starch. One amylolytic lactate-utilizing strain of Selenomonas ruminantium was also found. Two strains of Clostridium bifermentans were identified and showed proteolytic activity. Two strains were assigned to Mitsuokella jalaludinii and were saccharolytic. One strain belonged to a Bacillus species and seven strains were affiliated with Escherichia coli. All of the 20 strains were able to use many sugars, but none of them were able to degrade the polysaccharides carboxymethylcellulose and xylans. The effect of three propolis extracts (LLOS B1, C1 and C3) was tested on the in vitro growth of four representative isolates of S. bovis, E. coli, M. jalaludinii and C. bifermentans. The growth of S. bovis, E. coli and M. jalaludinii was not affected by the three propolis extracts at 1 mg ml(-1). C. bifermentans growth was completely inhibited at this LLOS concentration, but this bacterium was partially resistant at lower concentrations. LLOS C3, with the lower concentration of phenolic compounds, was a little less inhibitory than B1 and C1 on this strain.

The objective of this work was to evaluate the effects of Saccharomices cerevisiae and Lactobacillus casei in bovine diets, on dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) intake and digestibility, ruminal pH and ammonia nitrogen (N-NH3) and plasma urea nitrogen (PUN). Rations were isoproteic (13,04% CP) and isoenergetic (66,39% TDN) with sorghum silage used as roughage (50% of DM). Six ruminal-cannulated males, castrated, ½Gir½ Holstein crossbred, with average live wight of 317 kg and 24 months old were utilized. Treatments were determined as control (without probiotic addition) and with probiotic, which contained 4,5 x 109 CFU/animal/day of Saccaromyces cerevisiae and 3,1 x 107 CFU/animal/day of Lactobacillus casei. The experiment lasted 38 days, divided in two periods of 19 days. In the present work, the supply of live yeast Saccharomyces Cerevisiae and lactic bacteria Lactobacillus casei had no influence on DM, OM, CP, NDF and ADF intake and digestibility, ruminal pH, ruminal N-NH3 and PUN, indicating that can be used in cattle feed when necessary.

O objetivo deste trabalho foi caracterizar a variabilidade genética nos parentais (G0) e em três gerações consecutivas (G1, G2 e G3) de tilápia Gift (genetically improved farmed tilapia), por meio de marcadores microssatélites. Trezentos e sessenta indivíduos, provenientes do programa de melhoramento da Universidade Estadual de Maringá, foram selecionados quanto ao ganho de peso. O total de 21 alelos foi encontrado nos cinco loci microssatélites polimórficos (G12292, UNH140; G12311, UNH159; G12312, UNH160; G12314, UNH162; e G12315, UNH163), com número médio entre três e cinco alelos por locus. As frequências alélicas variaram de 0,017 (UNH160 - G2) a 0,750 (UNH160 - G0). A heterozigosidade média observada foi de 0,501, 0,391, 0,531 e 0,503 para G0, G1, G2 e G3, respectivamente. O coeficiente de endogamia médio foi 0,192 (G0), 0,401 (G1), 0,230 (G2) e 0,301 (G3). Todas as gerações apresentaram desvio no equilíbrio de Hardy-Weinberg, com desequilíbrio de ligação na maioria dos loci. Exceto para a G1, a heterozigosidade foi mantida nas gerações G2 e G3, o que indica que não há perda significativa de variabilidade genética no programa de melhoramento.

O objetivo deste trabalho foi estudar o comportamento ingestivo de bovinos da raca Nelore terminados em confinamento e alimentados com dietas contendo diferentes hibridos de milho. Foram utilizados 27 bovinos com peso vivo medio inicial de 350 ± 24 kg e idade media de 24 meses, distribuidos em delineamento inteiramente casualizado, em tres tratamentos (T), sendo, T1-DTMDU: Dieta total contendo milho duro, T2- DTMSDU: Dieta total contendo milho semiduro e T3-DTMSDE: Dieta total contendo milho semidentado, com nove repeticoes por tratamento. As dietas foram isoenergeticas e isoproteicas, com relacao de volumoso: concentrado de 30:70, utilizando-se o bagaco de cana como volumoso e racao concentrada composta de farelo de soja (8%), milho grao moido (88%), suplemento mineral e vitaminico (3%) e ureia (1%). Essas dietas foram fornecidas aos bovinos duas vezes ao dia (8 h e 16 h). Os animais foram confinados por um periodo de 95 dias, compreendendo 14 dias de adaptacao e tres periodos experimentais de 27 dias. Os bovinos foram pesados em jejum de solidos de 16 horas no inicio do experimento e ao final de cada periodo de 27 dias. A avaliacao do comportamento ingestivo ocorreu no ultimo dia de cada periodo experimental por meio de observacao visual dos animais a cada cinco minutos, por periodos integrais de 24 horas. Foram realizadas observacoes em quatro turnos: manha (06:00 as 12:00), tarde (12:00 as 18:00), noite (18:00 as 00:00) e madrugada (00:00 as 06:00) para determinar o numero de bolos ruminais, tempo de mastigacao, tempo de alimentacao, tempo de ruminacao em pe, tempo de ruminacao deitado, tempo de ocio em pe e tempo de ocio deitado. No periodo noturno o ambiente recebeu iluminacao artificial, sendo que tres dias anteriores a coleta de dados, os animais foram adaptados a essa luminosidade noturna. Houve diferenca entre os tratamentos para o tempo de mastigacao e para o numero de bolos ruminais. Animais alimentados com a dieta contendo milho semidentado apresentaram maior tempo de mastigacao e maior numero de bolos ruminais, do que aqueles alimentados com dieta contendo milho duro, entretanto nao diferiram daqueles que receberam milho semiduro na racao. Os tempos de mastigacao e os numeros de bolos ruminais variaram com os periodos de observacao (manha, tarde, noite e madrugada), sendo maior no periodo da manha e decrescente no periodo da tarde, noite e madrugada. Quanto as medias de tempo despendido para alimentacao, ruminacao e ocio, verificou-se que animais alimentados com racao contendo milho duro apresentaram maior tempo de alimentacao do que aqueles recebendo racao com milho semiduro ou semidentado. Entre as demais variaveis (ruminacao em pe, ruminacao deitado, ocio em pe e ocio deitado) nao houve diferenca significativa entre os animais recebendo diferentes dietas. Pode-se concluir que animais alimentados com dietas contendo milho semidentado apresentam maior tempo de mastigacao e maior numero de bolos ruminais do que aqueles alimentados com milho duro. Animais alimentados com dietas contendo milho duro na racao concentrada apresentam maior tempo de alimentacao do que aqueles recebendo dietas com milho semiduro ou semidentado. O tempo de mastigacao e o numero de bolos ruminais sao maiores no periodo da manha e decrescem no periodo da tarde, noite e madrugada.

This study aimed to determine the carbohydrate fractions and estimate ruminal fermentation kinetics parameters of silages Brachiaria brizantha cv. Marandu with the inclusion of two microbial additives: Additive LPPP Pasture (Lactobacillus plantarum and Pediococcus pentosaceus) and additive BSLPPA Pasture (Bacillus subtilis, Lactobacillus plantarum and Pediococcus acidilactici). The experimental design was completely randomized with three different silages and three replications per silage. The silos were opened after 34 days of fermentation and then sent for laboratory analysis. For the fractionation of carbohydrates were determined total carbohydrates (TC), non-fiber carbohydrates (NFC), fractions A + B1 (fractions of fast and average ruminal degradation), B2 fraction (fraction slowly degraded in the rumen) and fraction C (carbohydrates no digestible in the rumen). The gas production data were fitted to a logistic model bicompartimental. There was no difference in the values of the variables of fiber degradation (FC) kinetic and no-fiber carbohydrates (NFC) of the silages. However, silages Brachiaria brizantha cv. Marandu with use of additives LPPP and BSLPPA had higher fractions of digestible carbohydrates, reducing total carbohydrates and low indigestible fiber fraction of carbohydrates. Thus, the two microbial additives improved the nutritional value of silage Marandu-grass.

The objective of this study was to evaluate carcass characteristics and meat quality of lambs fed sunflower cake to replace the protein of cottonseed meal. The experiment was conducted at the sheep sector at the Farm School, State University of Londrina. Thirty Santa Ines lambs, intact males, with age and weight at the beginning of the experiment of 80 days and 21.45 +/- 2.16 kg, respectively, were used. It was used a completely randomized experimental design. The animals were divided into 5 treatments, with 6 replicates per treatment, according to the amount of protein of cottonseed meal replaced by protein of the sunflower cake (0, 20, 40, 60 and 80% of replacement). Carcasses were evaluated and the Longissimus dorsi was removed for analyzes of shear force, color, pH, marbling, water loss by pressure, sensory analysis, chemical analyses and lipid oxidation. Hot and cold carcass yields showed quadratic distributions, where the best values were found for treatments with low inclusion of sunflower cake. Carcass measurements, as well as cut yields were not affected by the content of sunflower cake. Regarding the parameters of lamb meat, only water losses during cooking and shear force were affected, being quadratic and linear, respectively. The meat was more tender in animals fed higher amounts of sunflower cake. The tasters of the sensory panel did not identify differences between treatments, and classified the meat with moderate odor intensity, good tenderness, low to moderate juiciness and medium acceptability. The replacement of cottonseed meal protein by sunflower cake protein in the levels used, did not affect the majority of the carcass characteristics and the meat quality.

The objective of this study was to assess the effects of five substitution levels of soybean meal by turnip forage cake in the concentrate, on dry matter intake (DM), organic matter (OM), crude protein (CP), ether extract (EE), neutral detergent fiber (NDF) and acid detergent fiber (ADF), pH and ammonia nitrogen (N-NH3) in the rumen liquid and plasmatic urea nitrogen (PUN) in beef steer. The diets were isoprotein (6.5 % CP) and isoenergetic (50.0% TDN), using in natura sugarcane silage as the only forage (85,5 % DM). Five castrated males were used, 1/2 Simental x Nelore cross, with average weight of 610 kg and 36 months old, all fistulated in the rumen. The different levels of replacement were: 0, 25, 50, 75 and 100%, based on CP responsible of soybean meal of ration. Each experimental period lasted 19 days. The experiment was carried out in a 5x5 latin square experimental design, with five animals and five periods. The potential of dry matter intake (%BW and g/kg BW0,75) of turnip forage cake forage was obtained with 27% of replacement in the protean basis in relation to soybean meal, promoting, a maximum intake of 0,217 kg/animal/day, not proportionating alterations in the ruminal dynamic and in the blood.

It was aimed in this work to evaluate bacterial strains tolerant to products based on propolis (LLOS) through the isolation, morphological and biochemical characterization techniques in diets with roughage:concentrate ratio 100:0 and 50:50. For roughage diets, the products LLOSC1 and LLOSB3 were evaluated, and for 50:50% diets, the products LLOSC1, LLOSD1, LLOSA2, and LLOSC3, which differed in alcoholic concentrations (1, 2 and 3) and propolis (A, B, C and D) concentrations. The ruminal liquid was anaerobically incubated at 39°C for 6 days in medium containing LLOS. After isolation, the strains were submitted to Gram staining and the bacterial growth was monitored by photospectrometer. It was evaluated the strain growth in the presence of the following subtracts: arabinose, cellulose, glucose, cellobiose, xylose, fructose, and lactose. In roughage diets, strains tolerant to LLOSC1 and LLOSB3 were similar to carbohydrates degradation, except lactose in which LLOSC1 was superior to strains tolerant to LLOSB3. For diets with 50:50 roughage:concentrate ratio, the products LLOSC3 and LLOSA2 stood out because they selected the highest number of strains able to degrade most of the tested carbohydrates. The results suggest that tolerance to propolis is higher in Gram-positive strains with several growth metabolic levels.Objetivou-se avaliar cepas bacterianas tolerantes a produtos à base de própolis pelas técnicas de isolamento, caracterização morfológica e bioquímica, em dietas com relação volumoso: concentrado de 100:0 e 50:50. Para dietas volumosas foram avaliados os produtos LLOSC1 e LLOSB3 e, para dietas 50:50% os produtos LLOSC1, LLOSD1, LLOSA2 e LLOSC3, diferentes quanto aos teores alcoólicos (1, 2 e 3) e as concentrações de própolis (A, B, C e D). O líquido ruminal foi incubado anaerobiamente a 39°C durante 6 dias em meio contendo LLOS. Após o isolamento, as cepas foram submetidas à coloração de Gram e o crescimento bacteriano foi monitorado por espectrofotômetro. Foi avaliado o crescimento das cepas na presença dos substratos: arabinose, celulose, glicose, celobiose, xilose, frutose e lactose. Em dietas volumosas as cepas tolerantes a LLOSC1 e LLOSB3 foram semelhantes na degradação dos carboidratos, exceto lactose no qual LLOSC1 foi superior às cepas tolerantes a LLOSB3. Para dieta 50:50 de volumoso:concentrado, destacaram-se os produtos LLOSC3 e LLOSA2, que selecionaram maior número de cepas capazes de degradar a maioria dos carboidratos testados. Os resultados sugerem que a tolerância à própolis foi maior nas cepas Gram-positivas, com níveis metabólicos de crescimento diversos.