Background Acetyl-CoA carboxylase (ACC) plays an important role in the metabolism of various cancer cells, but its role in head and neck squamous cell carcinoma (HNSCC) is uncertain. Therefore, in the present study, we explored the role of ACC2 in HNSCC. Methods Western blot and immunohistochemistry assays were used to determine ACC2 protein expression levels in laryngocarcinoma and adjacent normal tissues derived from patients with laryngocarcinoma. ACC2 expression was knocked down in the hypopharyngeal cancer cell line FaDu to determine its effect on apoptosis. Lipid oil red staining was used to test the change of intracellular lipid. Results The results showed that the ACC2 protein was highly expressed in laryngocarcinoma and that the ACC2 expression level was positively associated with the clinical cancer stage and negatively associated with the degree of laryngocarcinoma cell differentiation. Kaplan–Meier analyses indicated that compared with patients having low levels of ACC2, those with high ACC2 levels had a decreased 5-year survival rate. The results of western blot and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that knockdown of ACC2 accelerated apoptosis in FaDu cells. Furthermore, knockdown of ACC2 significantly reduced the intracellular lipid levels in FaDu cells. Conclusion These findings suggest that ACC2 may be an important prognostic marker for patients with HNSCC and that ACC2 may be a potential target in the treatment of HNSCC.

Cerebrovascular spasm is a life-threatening event in salt-sensitive hypertension. The relationship between store-operated calcium entry (SOCE) and vasoconstriction in hypertension has not been fully clarified. This study investigated the changes in cerebrovascular contractile responses in high salt intake-induced hypertension and the functional roles of the main components of SOCE, namely, polycystin-2 (TRPP2), stromal interaction molecule 1 (STIM1), and Orai3. Polycystic kidney disease 2 (which encodes TRPP2) knockout mice displayed decreased cerebrovascular SOCE-induced contraction. The blood pressure of age-matched rats fed a normal or high-salt diet for 4 weeks was monitored weekly using noninvasive tail-cuff plethysmography. The systolic blood pressure of the rats fed a high-salt diet was significantly higher than that of controls. Western blotting and immunohistochemical results showed that these hypertensive rats expressed higher levels of cerebrovascular TRPP2, STIM1, and Orai3 than controls. Cerebrovascular tension measurements of the basilar artery indicated that SOCE-mediated contraction was significantly increased in hypertensive rats compared with control rats. In addition, SOCE-mediated contraction was decreased in the basilar arteries of rats pretreated with the SOCE inhibitor BTP-2 (10 μM) or transfected with TRPP2-specific or STIM1-specific small interfering RNA. Staining with 2,3,5-triphenyltetrazolium chloride (TTC) was used to quantify the infarcted brain area 24 h after middle cerebral artery occlusion, a model of ischemic stroke, in rodents. The infarcted brain area was significantly greater in hypertensive rats and significantly lower in BTP-2-treated rats than in controls. Taken together, these findings indicate that SOCE-induced contraction may be overactive in the basilar arteries of salt-sensitive hypertensive rats, suggesting the dysregulation of TRPP2 and SOCE and its other components.

Background The elderly population is at risk of osteoarthritis (OA), a common, multifactorial, degenerative joint disease. Environmental, genetic, and epigenetic (such as DNA hydroxymethylation) factors may be involved in the etiology, development, and pathogenesis of OA. Here, comprehensive bioinformatic analyses were used to identify aberrantly hydroxymethylated differentially expressed genes and pathways in osteoarthritis to determine the underlying molecular mechanisms of osteoarthritis and susceptibility-related genes for osteoarthritis inheritance. Methods Gene expression microarray data, mRNA expression profile data, and a whole genome 5hmC dataset were obtained from the Gene Expression Omnibus repository. Differentially expressed genes with abnormal hydroxymethylation were identified by MATCH function. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the genes differentially expressed in OA were performed using Metascape and the KOBAS online tool, respectively. The protein–protein interaction network was built using STRING and visualized in Cytoscape, and the modular analysis of the network was performed using the Molecular Complex Detection app. Results In total, 104 hyperhydroxymethylated highly expressed genes and 14 hypohydroxymethylated genes with low expression were identified. Gene ontology analyses indicated that the biological functions of hyperhydroxymethylated highly expressed genes included skeletal system development, ossification, and bone development; KEGG pathway analysis showed enrichment in protein digestion and absorption, extracellular matrix–receptor interaction, and focal adhesion. The top 10 hub genes in the protein–protein interaction network were COL1A1, COL1A2, COL2A1, COL3A1, COL5A1, COL5A2, COL6A1, COL8A1, COL11A1, and COL24A1. All the aforementioned results are consistent with changes observed in OA. Conclusion After comprehensive bioinformatics analysis, we found aberrantly hydroxymethylated differentially expressed genes and pathways in OA. The top 10 hub genes may be useful hydroxymethylation analysis biomarkers to provide more accurate OA diagnoses and target genes for treatment of OA.

The heterogeneous cross-coupling reaction of aryl iodides with diphenylphosphine was achieved in DMAc at 130 degrees C in the presence of 1.0 mol% of MCM-41-supported tridentate nitrogen palladium(0) complex [MCM-41-3N-Pd(0)] with KOAc as base, yielding a variety of unsymmetrical triarylphosphines in good to excellent yields. The turnover frequency (TOF) of the catalyst can reach 30.67 h(-1). This new heterogeneous palladium(0) catalyst could easily be prepared by a simple procedure from commercially readily available reagents, and exhibited the same catalytic activity as homogeneous Pd(OAc)(2) or Pd(PPh3)(4), and could be recovered by filtration of the reaction solution and recycled at least seven times without significant loss of catalytic activity. (C) 2018 Elsevier B.V. All rights reserved.

A series of novel aromatic poly(ether ketone amide)s (PEKAs) were synthesized by the heterogeneous palladium-catalyzed carbonylative polycondensation of aromatic diiodides with ether ketone units, aromatic diamines, and carbon monoxide in N,N-dimethylacetamide (DMAc) at 120°C using 6 mol% of a magnetic nanoparticles–supported bidentate phosphine palladium complex (Fe3O4@SiO2-2P-PdCl2) as catalyst and 1,8-diazabicyclo[5,4,0]-7-undecene as base. The PEKAs had inherent viscosities ranging from 0.61 dl g−1 to 0.75 dl g−1. All the PEKAs were soluble in strong dipolar organic solvents. These PEKAs showed glass transition temperatures between 178°C and 232°C and 10% weight loss temperatures ranging from 443°C to 496°C in nitrogen. These PEKAs could be cast into transparent, flexible, and strong films from DMAc solutions with tensile strengths of 72.8–82.6 MPa, tensile moduli of 2.19–2.84 GPa, and elongations at break of 5.4–7.5%. Importantly, the heterogeneous palladium catalyst can be conveniently recovered from the reaction mixture by simply applying an external magnet and recycled up to eight times without significant loss of activity.

The novel MCM-41-immobilised nitrogen and sulfur mixed tridentate palladium(0) complex [MCM-41-2N,S-Pd(0)] was prepared from 3-(2-aminoethylamino) propyltrimethoxysilane by immobilisation on MCM-41, followed by reaction with thiophene-2-carboxaldehyde and PdCl2 and then reduction with hydrazine hydrate. This phosphine-free heterogeneous palladium(0) complex was a highly active catalyst for the Suzuki reaction of aryl bromides in air and could be recycled at least 8 times without significant loss of activity.

A highly efficient heterogeneous ruthenium(III)-catalyzed reaction of diaryl diselenides with alkyl halides was achieved in DMF at 100 degrees C in the presence of 2 mol% of an MCM-41-immobilized bidentate nitrogen ruthenium(III) complex [MCM-41-2N-RuCl3] and zinc, yielding a variety of unsymmetrical diorganyl selenides in good to excellent yields. This new heterogeneous ruthenium catalyst can easily be prepared via a simple two-step procedure from commercially readily available and inexpensive reagents, and recovered by filtration of the reaction solution and recycled for at least eight times without a significant loss of activity. (C) 2017 Elsevier B. V. All rights reserved.

Two monomers containing naphthalene, 4,4′-di(1-naphthoxy)benzophenone (DNOBN) and 4,4′-bis(1-naphthoxy)diphenyl sulfone (BNODPS), were prepared by the condensation reactions of 4,4′-difluorobenzophenone and 4,4′-dichlorodiphenyl sulfone with 1-naphthol, respectively. Novel poly(aryl ether ketone)s (PAEKs) and poly(aryl ether ketone sulfone)s containing 1,4-naphthylene units were synthesized from DNOBN and BNODPS via the electrophilic Friedel–Crafts acylation polycondensation with various aromatic diacid dichlorides, in the presence of anhydrous aluminum chloride and N-methyl-2-pyrrolidone in 1,2-dichloroethane. Because of the incorporation of 1,4-naphthylene units, the resulting PAEKs and poly(aryl ether ketone sulfone)s exhibited outstanding thermal stability and improved solubility in common organic solvents. The glass transition temperatures are above 200°C, and the temperatures at a 5% weight loss are above 548°C under nitrogen atmosphere. All these polymers are amorphous and can form strong, transparent, and flexible films with tensile strengths of 89.4–100.6 MPa, Young’s moduli of 2.13–2.65 GPa, and elongations at break of 15.5–25.3%. All these polymers had low dielectric constants of 2.70–2.89 at 1 MHz and low water sorption of 0.51–0.59%.

The Stille coupling of (E)-α-stannyl-α,β-unsaturated esters with alkynyl bromides in DMF in the presence of Pd(PPh3)4 and CuI gave (Z)-2-ethoxycarbonyl-1,3-enynes in good yields. The latter underwent the palladium-catalysed hydrostannylation with tributyltin hydride in THF at room temperature to afford (1E,3E)-2-ethoxycarbonyl-3- stannyl-1,3-dienes in good yields with high regio- and stereoselectivities.

The monomer containing sulfone and amide linkages, namely N,N'-bis(4-phenoxybenzoyl)-4,4'-diaminodiphenyl sulfone (BPBDAS), was conveniently prepared by the condensation of 4,4′-diaminodiphenyl sulfone with 4-phenoxybenzoyl chloride in N,N-dimethylacetamide (DMAc). Novel copolymers of poly (ether ketone biphenyl ketone ether ketone ketone) and poly (ether ketone sulfone amide) were synthesized by the modified Friedel-Crafts acylation solution copolycondensation of isophthaloyl chloride (IPC) with a mixture of 4,4′-bis (4-phenoxybenzoyl) biphenyl (BPOBBP) and BPBDAS. The copolymers obtained were characterized by different physico-chemical techniques. The copolymers with 10–25 mol% BPBDAS are semicrystalline and had increased T gs over the conventional PEEK and PEKK due to the incorporation of rigid biphenylene moieties, sulfone and amide linkages in the main chains. The copolymers III and IV with 20–25 mol% BPBDAS had not only high T gs of 182–185 °C, but also moderate T ms of 337–339 °C, having good potential for the melt processing. The copolymers III and IV had tensile strengths of 102.2–103.5 MPa, Young’s moduli of 2.65–2.76 GPa, and elongations at break of 17.5–18.9 % and exhibited high thermal stability and excellent resistance to organic solvents.

The heterogeneous formylation of aryl halides with HCO2Na at atmospheric pressure by carbon monoxide was readily achieved in the presence of the diphosphino-functionalised MCM-41-supported palladium complex in DMF to afford the corresponding aromatic aldehydes in good to excellent yields. This heterogeneous palladium catalyst can be recovered by simple filtration and reused 10 times without any loss of activity.

AbstractThe novel, practical and highly efficient catalyst system can be recovered by simple filtration and reused several times without any decrease in activity.

ChemInformVolume 44, Issue 17 Isocyclic Compounds ChemInform Abstract: Polystyrene-Immobilized Phenanthroline Palladium(II) Complex: A Highly Efficient and Recyclable Catalyst for Oxidative Heck Reactions. Pingping Wang, Pingping Wang Dep. Chem., Jiangxi Normal Univ., Nanchang 330027, Peop. Rep. ChinaSearch for more papers by this authorJiatao Zhang, Jiatao Zhang Dep. Chem., Jiangxi Normal Univ., Nanchang 330027, Peop. Rep. ChinaSearch for more papers by this authorMingzhong Cai, Mingzhong Cai Dep. Chem., Jiangxi Normal Univ., Nanchang 330027, Peop. Rep. ChinaSearch for more papers by this author Pingping Wang, Pingping Wang Dep. Chem., Jiangxi Normal Univ., Nanchang 330027, Peop. Rep. ChinaSearch for more papers by this authorJiatao Zhang, Jiatao Zhang Dep. Chem., Jiangxi Normal Univ., Nanchang 330027, Peop. Rep. ChinaSearch for more papers by this authorMingzhong Cai, Mingzhong Cai Dep. Chem., Jiangxi Normal Univ., Nanchang 330027, Peop. Rep. ChinaSearch for more papers by this author First published: 04 April 2013 https://doi.org/10.1002/chin.201317064Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article. Volume44, Issue17April 23, 2013 RelatedInformation

The Heck arylation reaction of aryl iodides with allylic alcohols catalysed by an mesoporous silica (MCM-41)-supported bidentate nitrogen palladium complex [MCM-41-2N-Pd(OAc) 2 ] proceeded smoothly in the presence of tetrabutylammonium chloride (TBAC) in DMF to afford β-aryl ketones in good to excellent yields. This heterogeneous palladium catalyst can be recovered by simple filtration and reused several times without any decrease in activity.

Mn2+ and RE3+ (RE = Tb, Eli, Nd) co-doped CdSiO3 orange phosphors were prepared at 1050 degrees C by a sol-gel method. The phase and crystallinity of the synthesized materials were investigated by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM). The luminescence characteristics were analyzed using photoluminescence (PL) spectra, afterglow decay curves, long-lasting phosphorescence spectra, and thermoluminescence (TL) spectra. Due to the difference in co-doped rare earth ionic radii, it varied greatly in trap density and trap depth caused by the different defects deriving from RE3+ ions co-doping into the CdSiO3: Mn2+ host. The afterglow intensity and time for these samples increased as follows: CdSiO3: Mn(2+)0.2%, Nd(3+)0.8% < CdSiO3: Mn(2+)0.4%, Tb(3+)0.8% < CdSiO3: Mn(2+)0.4%, Eu(3+)0.3%. CdSiO3: Mn(2+)0.4%, Eu(3+)0.3% had the best afterglow properties, which could be due to the proper traps formed by Eu3+ ions co-doping into the host. The role of RE3+ co-doped into the CdSiO3: Mn2+ matrix and the possible long-lasting phosphorescence process was also discussed in this paper. (C) 2012 Published by Elsevier Ltd.

The procedure is described as applicable to the stereocontrolled synthesis of naturally occurring 1,3-diene systems.

Oxidative Heck coupling reactions of arylboronic acids with olefins were carried out in the presence of the polystyrene-immobilised phenanthroline palladium(II) complex using air as a co-oxidant to afford the Heck coupling products in high yields under base-free conditions. This heterogeneous palladium catalyst can be recovered by simple filtration and used for several times without loss of activity or selectivity.

Nanocomposite films of poly (9,9-dioctylfluorene) (PFO)/Al2O3have been prepared by blending the conjugated polymer solution with alumina nanoparticle dispersion in common organic solvent and investigated in terms of the optical property. A blue shift of the photoluminescence (PL) spectrum for the as fabricated PFO/Al2O3nanocomposite film was observed as compared to the results obtained from the bulk PFO film, the maximum of the PL emission peaks occurs at 442 nm in the bulk film while after the incorporation of the alumina nanoparticles the maximum appears at 420 nm in the spectrum for PFO/Al2O3nanocomposite film. The dependence of fluorescence blue shift of PFO on the concentration of Al2O3was investigated. The isolation of the conjugated polymer chains by the flat rafts of alumina nanoparticles was shown to play a key role for the obtained blue shift.

Trisubstituted 1,3-dienes can be stereoselectively synthesized in one pot under mild conditions, in good yields, by hydromagnesiation of alkylarylacetylenes, followed by the palladium-catalyzed cross-coupling with alkenyl iodides.

AbstractThe tandem reaction proceeds in [bmim]BF4 and affords the (E)‐products with high yields.