BACKGROUND:Gastric cancer (GC) is the third most common cause of cancer-related death worldwide. Increasing studies have indicated that circular RNAs (circRNAs) play critical roles in cancer progression. However, the precise mechanism and functions of most circRNAs are still unknown in gastric cancer.METHODS:In the present study, we aim to uncover the mechanism by which circRNAs regulate gastric cancer tumorigenesis. By analyzing the microarray data, we screened differential expressed circRNAs in the gastric cancer group and identified a down-regulated circRNA, hsa_circ_0040039 (circSNTB2). Mechanically, circSNTB2 served as a sponge for the miR-6938-5p and up-regulated its expression.RESULTS:Meanwhile, G0/G1 switch gene 2 (G0S2) and programmed cell death gene 4 (PDCD4) were identified to be the aim genes of miR-6938-5p, constructing circSNTB2/miR-6938-5p/G0S2 and PDCD4 pathways.CONCLUSION:Taken together, our findings demonstrated that circSNTB2 plays an essential role in gastric cancer by regulating miR-6938-5p through G0S2 and PDCD4 genes. CircSNTB2 could be a promising biomarker for GC diagnosis and targeted therapy.

目的:观察散结片联合耳穴压豆治疗良性甲状腺结节的临床疗效.方法:将良性甲状腺结节患者90例,按随机对照原则分为空白对照组、中成药组、试验组,每组各30例.治疗过程共3个月,嘱患者调畅情志,放松身心,避免紧张焦虑.空白对照组仅随诊观察,中成药组给予小金丸口服1.2g/次,每日2次口服,试验组给予散结片3.0g/次,每日3次口服联合耳穴压豆(肝、脾、内分泌、皮质下、神门)治疗.观察治疗前后各组中医症状积分、证候总积分、证候疗效、结节最大横径、超声疗效、高危因素积分、安全性评价等指标,以评估临床疗效.结果:治疗后空白对照组各项观察指标较前均无明显改善.与中成药组比较,试验组除乳房胀痛症状改善无差异外,余各症状积分、证候总积分改善均明显优于中成药组(P＜0.05,P＜0.01);试验组证候疗效显效率、总有效率明显高于中成药组(P＜o.05,P＜0.01).试验组可明显缩小结节最大横径,降低高危因素积分,较中成药组显著提高超声总有效率及显效率(P＜0.05,P＜0.01).除空白对照组1例出现TSH轻度降低外,安全性评价为2级;余两组均无不良反应发生,安全性较高.结论:散结片联合耳穴压豆治疗良性甲状腺结节,临床疗效显著确切,值得进一步推广应用.

自的:观察微创搜刮法治疗肉芽肿性乳腺炎(GLM)的临床疗效.方法:收集山东中医药大学附属医院乳腺甲状腺外科宋爱莉教授治疗的肉芽肿性乳腺炎(脓肿期)患者,按照病例选择标准纳入76例,其中试验组39例,采用微创搜刮疗法;对照组37例,采用手术疗法.从治愈率、治疗时间、复发率、乳房外形、患者满意度等方面进行评价.结果:试验组总有效率为97.44％,对照组总有效率达100％,两组总有效率比较差异无统计学意义(P＞0.05).两组患者中位治疗时间比较差异有统计学意义(P＜0.05).试验组患者乳房外形优良率高于对照组,差异有统计学意义(P＜0.05).两组患者复发率比较差异无统计学意义(P＞0.05).两组患者非常满意率比较差异无统计学意义((P＞0.05).结论:微创搜刮法特点为创伤小、组织损失少,在患者治愈率、治疗时间及复发率、满意度方面无优势,但可以较好保持患者的乳房外形,减轻患者的生理、心理压力.

胆碱能抗炎通路(CAP)是一条以迷走神经、乙酰胆碱及特异性乙酰胆碱受体为基础的神经-免疫调节通路,与传统抗炎机制相比较,CAP抗炎作用更加快速、有效,并具有特异性.研究证实,中医药能够作用于CAP发挥抗炎作用.现就CAP的定义、抗炎机制以及中医药对CAP的影响进行综述.

目的探讨阳和化岩汤治疗乳腺癌的可能作用机制.方法通过SK-BR-3细胞株接种建立Balb/c裸鼠荷瘤模型,将20只成瘤阳性的裸鼠随机分为模型组、中药组、西药组、中西药组,每组5只.模型组每天灌胃0.4ml生理盐水;中药组用阳和化岩汤18 g/(kg·d)灌胃;西药组给予曲妥珠单抗1mg/kg,每周2次,腹腔注射;中西药组给予阳和化岩汤和曲妥珠单抗,用法同中药组和西药组.给药4周后观察各组淋巴结转移抑制率,检测肿瘤组织中磷脂酰肌醇-3羟基激酶(PI3K)、磷酸化丝氨酸/苏氨酸激酶(p-Akt)、血管内皮生长因子C (VEGFC)的表达,计数微淋巴管数量及VEGFC评分.结果各组裸鼠乳腺原位均有实体肿瘤生长,呈椭圆形或分叶状,原位移植乳腺癌模型裸鼠均有腋窝淋巴结转移.与模型组比较,各给药组淋巴结抑制率均上升,VEGFC评分及PI3K、p-Akt、VEGFC mRNA表达均降低,微淋巴管数量减少(P＜0.05或P＜0.01);与中药组、西药组比较,中西药组淋巴结抑制率明显升高,VEGFC评分及PI3K、p-Akt、VEGFC mRNA表达均降低,微淋巴管数量减少(P＜0.05或P＜0.01).VEGFC评分与微淋巴管数量之间存在正相关关系(r =0.894,P＜0.05). 结论 阳和化岩汤能有效抑制HER-2高表达型裸鼠荷瘤模型微淋巴管生成,抑制血管生成调控通路及VEGFC表达,其机制可能与有效抑制H3K/Akt交互调控通路活化有关.

目的:探讨阳和化岩汤与胞内磷脂酰肌醇激酶(PI3K)抑制剂LY294002对人表皮生长因子(HER-2)高表达型裸鼠荷瘤肿瘤及微淋巴管密度(LMVD)生成的干预作用及机制的研究.方法:建立裸鼠荷瘤模型,随机分为对照组、阳和化岩汤18g/kg组、LY294002 7.5mg/kg组、LY294002 7.5 mg/kg+阳和化岩汤18g/kg组,药物干预4周后处死裸鼠,取出肿瘤标本及腋下淋巴结,计算抑瘤率和淋巴结转移抑制率.免疫组化法观察内皮生长因子C(VEGFC)表达,Podoplanin抗体标记淋巴管内皮,计数微淋巴管密度(LMVD),观察微淋巴管在癌灶及周围间质组织分布特点及形态学结构改变,RT-PCR法检测分析关键靶蛋白PBK、磷酸化蛋白激酶B(p-Akt)、VEGFC的表达.结果:与对照组相比,阳和化岩汤18g/kg组、LY294002 7.5mg/kg组、LY294002 7.5mg/kg+阳和化岩汤18g/kg组抑瘤率与淋巴结转移抑制率均不同程度增加;镜下观察VEGFC与LMVD染色,对照组染色程度最高,分布密集,阳和化岩汤组、LY294002组次之,LY294002+阳和化岩汤组染色程度最浅,分布最少;各用药组均可降低PI3K、p-Akt、VEGFC的表达,阳和化岩汤组的效果不及LY294002组,LY294002+阳和化岩汤组作用最明显.结论:阳和化岩汤、LY294002能有效抑制肿瘤生成及淋巴结转移,与抑制PI3K/Akt通路活化及VEGFC相关.虽然阳和化岩汤组对抑制PBK、p-Akt、VEGFC的能力不如LY294002组,但两者联用体现了良好的治疗效果.

Objective:To summarize the common feature and the syndrome difference of Hashimoto's thyroiditis through the syndrome standardization research,and provide the evidence for clinical TCM syndrome diagnosis.Methods:The clinical symptoms and signs of 117 Hashimoto's thyroiditis patients were collected,and the database was then built to converse these four diagnostic information to count data.The common TCM syndrome manifestations and syndrome differentiation were summarized by frequency count analysis and cluster analysis.Results:In 117 patients,there were 109 cases of female and 8 cases of male.The average age was (39±12) years old,and the average disease duration was (15.3±16.8) months.In 52 syndrome factors,the 5 most frequent signs were cervical enlargement,fatigue,burnout,irritability and sweating;the most common pulse types were thready pulse,weak pulse and stringy pulse;the most common tongue signs were plump tongue,teeth-printed tongue,and red tongue,and thin yellow or thin white fur.The results of cluster analysis showed that there were three syndrome differentiations that were the syndrome of liver depression and qi stagnation,syndrome of yang deficiency of spleen and kidney and syndrome of exuberant fire due to yin deficiency.The results of thyroid function test showed that syndrome of exuberant fire due to yin deficiency was often diagnosed as hyperthyroidism,the syndrome of liver depression and qi stagnation was often diagnosed as normal TSH,and the syndrome of yang deficiency of spleen and kidney was often diagnosed as hypothyroidism.Conclusion:With the illness degree,Hashimoto's thyroiditis is diferenfiated in three syndromes for three stages:syndrome of hyperactivity fire due to yin deficiency for early stage,syndrome of liver depression and qi stagnation for secondary stage,syndrome of yang deficiency of spleen and kidney for late stage.

乳腺增生病是最常见的乳腺疾患,属于中医学乳癖范畴.由于该病病机复杂、缺乏客观统一的辨证量化标准,故目前该病中医辨证分型多元化,且临床大多病例证候兼杂.宋爱莉教授根据多年治疗乳腺增生病的经验,认为痰瘀互结为乳腺增生病的主要病机.

Summarizing the recognization of modern and traditional medicine to breast cancer total correction,and medical research of blood-activiating and stasis-dissolving drugs,providing theory foundation for clinic application.