Thermochemical processes have recently attracted more attentions from researchers, particularly the use of decomposition of ammonia-based compounds and water-gas shift and steam reforming processes. These processes may be applied for hydrogen generation (H2) 2 ) from a syngas mixture and that is the main concern of this paper. Furthermore, these processes are currently facing the challenge of low hydrogen yields. Traditionally, H2 2 fuel is generated from fossil fuels by utilizing different reforming processes. The longevity and reliability of catalysts, their purity and the start-up time/costs are also some of the critical challenges to be faced. Sustainable H2 2 generation from sustainable sources like ammonia (NH3) 3 ) gas was found to be an innovative process with the appropriate selection of the catalytic process and also thermochemical processes being shown to be critical issues. Traditional approaches to H2 2 synthesis such as the ammonia cracking process and the generation of hydrogen occur at high temperatures of 500oC o C or more. Current efforts in the field of H2 2 generation rely on a process which includes the decomposition of ammonia gas, this achieves a high yield through the application of alkali metal amide/imides as effective catalysts. NH3 3 and methane (CH4) 4 ) decomposition is exploited for H2 2 production through the application of a steam methane reforming process in bench scale packed-bed reactors. This requires an NH3 3 feed solution at a temperature of 150oC o C that can generate more H2 2 (up to 90%) in the total syngas yield. Efforts were made to change/shift the equilibrium towards increased hydrogen production. Researchers have engaged in many efforts to remove H2 2 from a membrane or in carbon dioxide extraction with the use of a solid sorbent. A continuous mode of enhanced H2 2 production can be achieved by integrating the reforming process with concentrated solar radiation for thermal storage. Efforts were made to remove H2 2 from a membrane or extract carbon dioxide using a solid sorbent. This review explores the decomposition techniques, catalytic systems and thermochemical conditions required for H2 2 generation from NH3/NH3-rich 3 /NH 3-rich products/wastes.

The paper introduces the analysis results over a selected number of small-scale farms in the Western Romanian agriculture, more precisely in the counties of Arad and Timis. The purpose of the investigation is linked to the assessment of need for advisory services input in solving the problems of the farmers, particularly for the ones operating in small farms. Analysing the returned answers in connection with the fields of interest or needed expertise, accounting for the forms of mutual or directional help, compiling any forms of advice inputs, including the specialised structures other than public in absence of a function Farm Advisory Service allow the development of a structured image in terms of current problems, the way of solving and the interactions among the farmers inside or outside their communities. The quantified findings can serve as base for a public policy recommendation in the field of farm advisory as part of the Agriculture Knowledge and Innovation System.

The digitalization of the agricultural industry is manifested through the active use of innovative technologies in all its areas. Agribusiness owners have to constantly improve their security to meet new challenges. In this context, the existing cyber risks of the agrarian industry were assessed and their classification by possible consequences, such as data theft or alteration, cyber terrorism, cyber warfare, software hacking or modification, the blocking of markets and transactions on them, was proposed. Cyber insurance is an effective tool for minimizing the likelihood of cyber incidents and for comprehensive post-incident support, with the involvement of cybersecurity specialists. An algorithm for cooperation between an agricultural company and an insurance company when concluding a cyber risk insurance contract is proposed, which takes into account the needs and wishes of insurers at each stage of the interaction. To assess the need to use cyber insurance in agriculture 4.0, a methodology has been developed to evaluate the regional characteristics of cybersecurity and the digitalization of agribusiness. The results of the study show a heterogeneous need for this tool in different regions of the world.

This study investigated the phytochemical characteristics, antibacterial activity, and synergistic potential of essential oils derived from Romanian lavender. Gas Chromatography–Mass Spectrometry (GC/MS) analysis revealed that linalool is the main compound in all lavender essential oils, with concentrations ranging from 29.410% to 35.769%. Linalyl acetate was found in similar concentrations to linalool. Other significant compounds included 1,8-cineole (8.50%), lavandulyl acetate (5.38%), trans-β-ocimene (6.90%), and camphor (7.7%). A 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) test was used to assess antioxidant capacity, with substantial free-radical-scavenging activity shown in the IC50 values determined. The antibacterial efficacy of the oils was higher against Gram-positive bacteria than Gram-negative bacteria, with variations in minimum inhibitory concentrations (MICs), the extent of inhibition, and evolution patterns. The study also explored the oils’ ability to enhance the efficacy of ampicillin, revealing synergistic interactions expressed as fractional inhibitory concentration indices. In silico protein–ligand docking studies used twenty-one compounds identified by GC-MS with bacterial protein targets, showing notable binding interactions with SasG (−6.3 kcal/mol to −4.6 kcal/mol) and KAS III (−6.2 kcal/mol to −4.9 kcal/mol). Overall, the results indicate that Romanian lavender essential oils possess potent antioxidant and antibacterial properties, and their synergistic interaction with ampicillin has potential for enhancing antibiotic therapies.

Salt stress is a major abiotic stressor that limits plant growth, development, and agricultural productivity, especially in regions with high soil salinity. With the increasing salinization of soils due to climate change, developing salt-tolerant crops has become essential for ensuring food security. This review consolidates recent advances in plant genetics, transcription factors (TFs), and next-generation sequencing (NGS) technologies that are pivotal for enhancing salt stress tolerance in crops. It highlights critical genes involved in ion homeostasis, osmotic adjustment, and stress signaling pathways, which contribute to plant resilience under saline conditions. Additionally, specific TF families, such as DREB, NAC (NAM, ATAF, and CUC), and WRKY, are explored for their roles in activating salt-responsive gene networks. By leveraging NGS technologies—including genome-wide association studies (GWASs) and RNA sequencing (RNA-seq)—this review provides insights into the complex genetic basis of salt tolerance, identifying novel genes and regulatory networks that underpin adaptive responses. Emphasizing the integration of genetic tools, TF research, and NGS, this review presents a comprehensive framework for accelerating the development of salt-tolerant crops, contributing to sustainable agriculture in saline-prone areas.

Abstract Environmental challenges related to plastic waste underscore the urgent need for innovative solutions. Polyhydroxyalkanoates (PHA) have emerged as sustainable alternatives to conventional plastics, particularly in packaging, due to their biodegradability and biocompatibility. However, the cost of PHA production and certain physical limitations compared to synthetic polymers remain significant barriers to widespread adoption. Within this context, Actinophytocola algeriensis, an Actinobacteria species isolated from the Sahara desert in Algeria, holds promise for its biotechnological potential and bioactive molecules. Despite this, our understanding of its enzyme profile, notably the PHA synthase (EC 2.3.1.304), the key enzyme in PHA biosynthesis, remains limited. In this study, the 3D structure of PHA synthase was modeled utilizing the artificial intelligence program AlphaFold, followed by the structural refinement and validation. In addition, physicochemical properties and functional characterization were conducted using various bioinformatics tools. This research signifies a substantial advancement in comprehending the molecular mechanisms underlying PHA biosynthesis in A. algeriensis, thereby fostering the development of innovative biotechnological applications for sustainable biopolymer production.

Heavy metal contamination threatens the aquatic environment and human health. Different physical and chemical procedures have been adopted in many regions; however, their adoption is usually limited since they take longer time, are more expensive, and are ineffective in polluted areas with high heavy metal contents. Thus, biological remediation is considered a suitable applicable method for treating contaminates due to its aquatic-friendly features. Bacteria possess an active metabolism that enables them to thrive and develop in highly contaminated water bodies with arsenic (As). They achieve this by utilizing their genetic structure to selectively target As and deactivate its toxic influences. Therefore, this review extensively inspects the bacterial reactions and interactions with As. In addition, this literature demonstrated the potential of certain genetically engineered bacterial strains to upregulate the expression and activity of specific genes associated with As detoxification. The As resistant mechanisms in bacteria exhibit significant variation depending on the genetics and type of the bacterium, which is strongly affected by the physical water criteria of their surrounding aquatic environment. Moreover, this literature has attempted to establish scientific connections between existing knowledge and suggested sustainable methods for removing As from aquatic bodies by utilizing genetically engineered bacterial strains. We shall outline the primary techniques employed by bacteria to bioremediate As from aquatic environments. Additionally, we will define the primary obstacles that face the wide application of genetically modified bacterial strains for As bioremediation in open water bodies. This review can serve as a target for future studies aiming to implement real-time bioremediation techniques. In addition, potential synergies between the bioremediation technology and other techniques are suggested, which can be employed for As bioremediation.

Multiple drug resistance (MDR) has gained pronounced attention among Enterobacterales. The transfer of multiple antimicrobial resistance genes, frequently carried on conjugative incompatibility F (IncF) plasmids and facilitating interspecies resistance transmission, has been linked to Salmonella spp. and E. coli in broilers. In Egypt, the growing resistance is exacerbated by the limited clinical efficacy of many antimicrobials. In this study, IncF groups were screened and characterized in drug-resistant Salmonella spp. and E. coli isolated from broilers. The antimicrobial resistance profile, PCR-based replicon typing of bacterial isolates pre- and post-plasmid curing, and IncF replicon allele sequence typing were investigated. Five isolates of E. coli (5/31; 16.13%) and Salmonella spp. (5/36; 13.89%) were pan-susceptible to the examined antimicrobial agents, and 85.07% of tested isolates were MDR and extensively drug-resistant (XDR). Twelve MDR and XDR E. coli and Salmonella spp. isolates were examined for the existence of IncF replicons (FII, FIA, and FIB). They shared resistance to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanate, doxycycline, cefotaxime, and colistin. All isolates carried from one to two IncF replicons. The FII-FIA-FIB+ and FII-FIA+FIB- were the predominant replicon patterns. FIB was the most frequently detected replicon after plasmid curing. Three XDR E. coli isolates that were resistant to 12–14 antimicrobials carried a newly FIB replicon allele with four nucleotide substitutions: C99→A, G112→T, C113→T, and G114→A. These findings suggest that broilers are a significant reservoir of IncF replicons with highly divergent IncF-FIB plasmid incompatibility groups circulating among XDR Enterobacterales. Supporting these data with additional comprehensive epidemiological studies involving replicons other than the IncF can provide insights for implementing efficient policies to prevent the spreading of new replicons to humans.

This study aims to evaluate the in silico genomic characteristics of five species of the genus Planotetraspora: P. kaengkrachanensis, P. mira, P. phitsanulokensis, P. silvatica, and P. thailandica, with a view to their application in therapeutic research. The 16S rRNA comparison indicated that these species were phylogenetically distinct. Pairwise comparisons of digital DNA-DNA hybridization (dDDH) and OrthoANI values between these studied type strains indicated that dDDH values were below 62.5%, while OrthoANI values were lower than 95.3%, suggesting that the five species represent distinct genomospecies. These results were consistent with the phylogenomic study based on core genes and the pangenome analysis of these five species within the genus Planotetraspora. However, the genome annotation showed some differences between these species, such as variations in the number of subsystem category distributions across whole genomes (ranging between 1979 and 2024). Additionally, the number of CAZYme (Carbohydrate-Active enZYme) genes ranged between 298 and 325, highlighting the potential of these bacteria for therapeutic research applications. The in silico physico-chemical characteristics of cellulases from Planotetraspora species were analyzed. Their 3D structure was modeled, refined, and validated. A molecular docking analysis of this cellulase protein structural model was conducted with cellobiose, cellotetraose, laminaribiose, carboxymethyl cellulose, glucose, and xylose ligand. Our study revealed significant interaction between the Planotetraspora cellulase and cellotetraose substrate, evidenced by stable binding energies. This suggests that this bacterial enzyme holds great potential for utilizing cellotetraose as a substrate in various applications. This study enriches our understanding of the potential applications of Planotetraspora species in therapeutic research.

If a few years ago climate change was a new and fanciful concept and not everyone was aware of its entire action range, today we are witnessing increasingly pronounced climate change. At the European Union level are many political initiatives in order to mitigate the climate change so the next generations to have the same changes to a good environment. All the efforts must be put into shifting the development to a more sustainable one. There are different drivers that interfere with the climate. Among energy, transport, industrial processes, waste, land use, we also find agriculture as a driver for climate change. In agriculture the livestock, the utilized agricultural area, the type of farms depending on the input level, the consumption of inorganic fertilizers influences the climate, either through green gas emissions, water and air pollution, desertification etc. So, we could say that there are many aspects of agriculture that influence climate change. In the work, a brief screening of the aspects of agriculture that have a negative impact on the environment at the level of the European Union was carried out.

Abstract Actinoalloteichus hoggarensis is a rare bacterial species that was isolated from the Algerian Saharan desert and is known for producing biologically active compounds. Despite its potential, little is understood about the enzymes it produces, including endoglucanases. These cellulase enzymes break down cellulose, the primary structural component of plant cell walls that provides strength and rigidity. The breakdown of cellulose by endoglucanases has numerous biotechnological applications, such as the production of biofuels, bioplastics, and paper. This study involves an in silico characterization of an endoglucanase from A. hoggarensis to gain insight into its structural and functional properties, with the goal of informing the development of novel biotechnological applications. Our study represents a major milestone in understanding the potential of this rare bacterial species and its enzymes, opening up exciting new avenues for further research and development.

The current scientific paper presents a review of essential oils with antifungal effect, the mechanism of action of the main components of essential oils and the possible synergistic actions between them on pathogens. Essential oils of oregano, cinnamon, fennel, mint and dill in various concentrations have demonstrated effects on ergosterol biosynthesis, specifically reducing the amount of ergosterol. Thus, a significant change in ergosterol biosynthesis will inhibit the growth of fungi and cause their death. An advantage of using essential oils over synthetic ones is the use of small amounts of essential oils to achieve strong fungistatic and fungicidal effects. In conclusion, through this work we tried to make: an enumeration / revision of volatile oils with bio pesticidal potential, highlighting the effects produced on fungi from the Aspergillus, Fusarium and Penicillium families, but also on the mycotoxins biosynthesized by them.

This study aimed to explore and compare Polish and Romanian students’ opinions towards the integration of learning games in Higher Education subjects and to highlight the improved soft skills by participating in learning games. This article reports the results of a quantitative study that was designed to explore and compare the opinions of 103 students from 2 universities towards the integration of learning games in the Project Management subject. The students ranked 71 items found in the questionnaire in 7 scale questions. The items are presented in the form of skills that can be developed by participating in educational games and aspects that are important in Project Management. In the analysis of the data, the T test—two sample test—was used, and the nonparametric Wilcoxon rank-sum test, using the SAS Studio application to assess whether the average responses are different between the two groups (α = 0.05). There were significant differences in the case of some items related to the skills that can be developed by participating in two educational games (communication, empathy, awareness, problem-solving, work productivity, and time management), but also items that help students understand the importance of some aspects related to Project Management after participation in educational games (work productivity, team dimension, time management, and following request).

Water pollution and climate change are two of the most pressing environmental challenges of our time, and their interconnection is increasingly evident. Water pollution and climate change are interconnected in various ways. The release of greenhouse gases, primarily carbon dioxide, methane, and nitrous oxide, is a significant driver of global warming. These gases contribute to the greenhouse effect, leading to rising global temperatures, which, in turn, affect water resources. Warmer temperatures exacerbate water pollution by increasing the prevalence of harmful algal blooms, the degradation of water quality, and disruptions in aquatic ecosystems. Additionally, climate change-induced extreme weather events like floods and droughts can intensify water pollution by causing the runoff of pollutants into water bodies. Preventing water pollution is an essential component of mitigating climate change. Furthermore, the preservation of healthy ecosystems plays a vital role in both preventing water pollution and addressing climate change. Wetlands and forests act as carbon sinks, sequestering carbon dioxide, while also filtering pollutants from runoff. Protecting and restoring these ecosystems is a win-win strategy for combatting both issues simultaneously. These global challenges require integrated strategies that recognize the symbiotic relationship between them. By implementing effective pollution control measures, reducing carbon emissions, and protecting vital ecosystems, we can make significant strides in combating these urgent issues and ensuring a sustainable future for our planet.

The COVID-19 pandemic and the resulting public health crisis had an enormous impact on the global economy and its sectors. Most components were adversely affected, especially the tertiary industry (the part of a country’s economy that provides services), with different types and sizes of businesses suffering to varying degrees. Reports on the impact on agriculture are not lacking, and the crisis was perceived and responded to differently from the supply chain to the household level. The research question proposed in this paper concerns the impact of the early restrictions induced by COVID-19, namely the lockdown period from March to June 2020, on Romanian agriculture and more precisely Western Romanian crop production. Two counties in the West were selected: the county Timis for its highly favourable agricultural production and the county Caras Severin for its integrative agricultural production centred on mixed farms, where crop production is integrated with animal production towards a higher added value. Using secondary data from the National Agency for Payments and Interventions in Agriculture allowed the disaggregation of data at the level of each municipality for 2019 and 2020. The choice of this dataset was related to the level of precision, as the beneficiaries of direct payments in the respective areas are verified each year for both areas and crops. The paired two-tailed t-test was used to test the data for each LAU 2 municipality in each of the selected counties; as the crops sown in 2019 could not be affected by the COVID lockdown period in spring 2020 (March to June), the most important spring crops in terms of area in 2020 were selected and tested against the 2019 datasets. The results show that there is not enough evidence to conclude a significant statistical difference between the two years, and therefore, we cannot reject the null hypothesis and conclude that the pandemic lockdown did not affect the spring crops during their most restrictive period in spring 2020; thus, the overall influence of the COVID-19 lockdown on crop production in Western Romania was insignificant, observed only at the level of primary production. Spring crop production could have been severely affected by the restrictions imposed by the health crisis on access to certain inputs, freedom of movement for field work, and other activities closely linked to agricultural production, leading to early disruptions along the food chain.

Salt stress induces cytotoxicity at the cellular level, influencing the vacuolization process, disrupting mitotic division, and thus inhibiting plant growth. The results for a range of species used in agriculture have shown that high soil salt levels affect germination, chlorophyl content and yield. In this study, an experiment was carried out in the laboratory using NaCl concentration treatments of 0, 100, 125 and 150 mM on sunflower seeds of the inbred line HA-89 obtained from the USDA gene bank. For the experiment, the seeds were germinated in a salt solution and analyzed cytologically by calculating the mitotic index, chromosomal aberration index, provacuolar index and vacuolization index. Following our cytological studies, we observed that the vacuolization phenomenon was caused by salt stress and progressively accentuated by the salt concentration levels and exposure times. The formation of vacuolized cells is due to the fusion of provacuoles, which contributes to a uniform or non-uniform distribution of genetic material around them. According to our results, the vacuolization index showed high values depending on the NaCl concentration and stress exposure time. Similarly, high salt concentrations significantly decreased the mitotic index and increased the chromosomal aberration index. The effect of salt stress causes cell vacuolization, a decrease in the mitotic index and an increase in the number of chromosomal aberrations in meristematic tissues, inhibiting growth and development and consequently leading to a reduction in productivity per unit area.

Plant biodiversity data are prerequisites for the sustainable management of a forest. We used quantitative ecological tools to determine the species composition, diversity (richness and evenness), population structure, distribution patterns, and regeneration status of trees in a Tropical Moist Sal Forest of Eastern Ghats, India. For this purpose, a field inventory was conducted during 2020–2022 in sixteen 1.0 ha forest stands along a human-induced disturbance gradient. A total of 161 species (61 trees, 40 shrubs, 60 herbs) belonging to 77 families and 143 genera were recorded in the 16.0 ha of forest area. The results revealed a significant (p < 0.01) decrease in the tree and shrub species density, basal area, species richness, and diversity along the gradient of disturbance. However, in the case of herbs, the Shannon–Weiner diversity index increased significantly (p < 0.01) with increasing disturbance levels. Irrespective of forest types and disturbance levels, the tree diameter class (10–30 cm) accounted for the highest stem density in the forest. A mixed trend was found in the case of the basal area; the >41 cm diameter class constituted the highest basal area in the Pure Sal Forest while the 10–30 cm class was in the Moist Deciduous Forest without Sal. Tree species richness was found higher in lower diameter classes. Disturbances impacted the distribution pattern of trees; in the Pure Sal Forest, the contagious distribution of trees were 61.54%, 40%, and 12.5% in undisturbed, low-disturbed, and moderately disturbed sites, respectively. The percent of trees showing random and regular distribution increased with the increased level of disturbance in all forest types. The number of tree species having good regeneration decreased with the increased disturbance intensity in all forest types. Frequent grazing, repeated forest fires, and poor soil seed banks at the Moderately Disturbed site were the main reasons for the poor/no regeneration of Pterocarpus marsupium, Adina cordifolia, Terminalia bellerica, and some other economical species. Significant changes in structural attributes of the tree community revealed the impact of human-induced disturbances in the Moist Sal Forests of Eastern Ghats. The disturbance mosaics promoted the growth of many invasive weed species and lianas, depleting the number of valuable species in the forest. This study suggests the adaption of sustainable biodiversity conservation approaches through the active participation of the tribal so that the remnants the Moist Sal Forests of Eastern Ghats can be controlled to prevent further degradation.

The increasing utilization of nanoparticles (NPs) in agricultural practices has led to a surge in demand for nano-based products. Herein, we investigate the dose-dependent impacts of nickel hydroxide (Ni(OH)2)/nickel (Ni) NPs, synthesized using Asparagus racemosus Linn. leaf extract, on the seed germination and growth of Vigna radiata (Linn.) Wilczek. In all seed samples, 100% germination was observed in Treatment 1 (2.74 mg mL−1) and Treatment 2 (5.48 mg mL−1) of Ni/Ni(OH)2 NPs. However, in Treatment 3 (8.22 mg mL−1) and Treatment 4 (10.96 mg mL−1), the germination percentage was lower, reaching 80%. Further, Treatment 5 (13.70 mg mL−1) of Ni/Ni(OH)2 NPs showed a reduced germination rate of 60%, indicating a prolonged germination process at higher concentrations. Remarkably, the length of seedlings showed a significant increase in all experimental treatments compared to the control group, which received 5 mL of distilled water. Among the investigated parameters, Treatment 2 demonstrated the most promising outcomes, exhibiting the highest chlorophyll stability index (23.73%) and membrane stability index (67.89%) values, as well as the lowest root ion leakage (24.75%). These findings indicate that Ni/Ni(OH)2 has the capacity to enhance seed germination and foster seedling growth.

Climate change exerts a substantial influence on global livelihood security. This research aims to elucidate the risk faced by agroforestry managers of urban and rural areas. Adhering to the IPCC risk framework, we structured the experimental design and adopted an indicator-based methodology to delineate the risk dimensions. Altogether, 105 households from 7 villages in Aizawl district, Mizoram, India, were considered for the study. For indicator identification, we conducted a comprehensive literature review and subsequently employed principal component analysis to select relevant indicators. Finally, risk was determined using the index value of hazard, exposure, and vulnerability. Additionally, we also developed a regression model and integrated it into ArcGIS to generate a spatial risk map. Out of 69 indicators identified, 52 were selected for final assessment after PCA analysis. Our findings underscore the higher susceptibility of urban agroforestry managers to climate change which was in agreement to our hypothesis that the risk index of agroforestry households increases with altitude while it decreases with the distance from Aizawl headquarter. Furthermore, we observed that households residing at higher altitudes exhibit greater vulnerability. Key determinants contributing to elevated risk in the region encompass land ownership constraints, diminished yields, traditional farming practices with no institutional help, and a dearth of available labour resources. The study advocates the implementation of climate smart agroforestry practices integrated with agricultural credit schemes and an educational policy designed to enrol dropout youths.

In the era of digitization, in a context where climate changes affect all sectors of activities and life itself, modern translation has a crucial role and a major importance in all the activities related to the prevention and education of young generations and not only, from the modern societies to the disadvantaged backgrounds. The most valuable and important information, as well as all the EU regulations, procedures, resources, manuals, books, may be transmitted nowadays through translation worldwide. Using modern tools, modern CAT tools, huge amount of data may be converted from one language to several ones in a very short time. It is of a great importance that all the educational process related to climate change, all the measures, especially for the actual and future generations from several disadvantaged countries where air pollution it is increased over-passing the limits and threatening environment and society, may be translated into the national languages so it may be available and also possible to be taught. The acknowledgement of the danger of air pollution and climate change may reach through translation to a very large scale of the population.