Abstract: https://doi.org/10.22029/jlupub‐19591

Copper is an essential trace element, yet concerns regarding its toxic potential have gained increasing attention, particularly with regard to nanoparticulate forms of copper oxides. Both CuO and Cu2O are currently authorised in the EU for use in agriculture, biocides, and animal nutrition, but regulatory clarity regarding their nanoforms remains limited. This comprehensive review—combining narrative, bibliometric, and systematic approaches—addressed three key questions: (i) whether CuO and Cu2O nanoparticles are sufficiently considered within EU regulations to prevent unintentional oral exposure via the food and feed chain, (ii) whether these nanoparticles can cross the intestinal barrier as intact particles and accumulate in tissues, and (iii) whether they elicit distinct biological responses, including regulated cell death pathways such as cuproptosis. With respect to question (i), a detailed analysis of EU legal frameworks revealed a heterogeneous regulatory landscape and a lack of binding provisions that would preclude unintended presence of copper oxide nanoforms in regulated materials. Although some nano-specific data requirements exist, empirical information on nanoparticulate fractions in commercial products is still lacking. With respect to questions (ii) and (iii), our systematic literature analysis supports consistent intracellular uptake of CuO and Cu2O nanoparticles, yet direct evidence for transport of intact particles across the intestinal barrier is scarce. Mechanistic evidence supports cuproptosis as a key pathway for CuO nanoparticle toxicity, while the extent to which these effects differ quantitatively from ionic copper remains unclear due to insufficient data using appropriate experimental controls. Notably, a pronounced data gap was identified for Cu2O nanoparticles across all evaluated domains. Finally, to organise and integrate the mechanistic evidence presented, we developed an adverse outcome pathway (AOP), registered as AOP 590 in the AOP Wiki, describing how increased intracellular copper can induce cuproptosis via disruption of energy metabolism. Collectively, these findings highlight the need for coordinated analytical, mechanistic, and regulatory research to support evidence-based risk assessment of CuO and Cu2O nanoparticles in the food and feed context.

The objective of this study was to evaluate whether ensiling reduces tropane alkaloid concentrations in whole-plant maize contaminated with Datura stramonium, a concern for animal feed safety. Whole-plant maize silages were prepared with 10

Polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs), commonly known as “dioxins”, and polychlorinated biphenyls (PCBs) are persistent, environmentally ubiquitous lipophilic contaminants. Most human exposure comes from animal products, such as milk and meat. In this study, a whole-body physiologically-based toxicokinetic (wbPBTK) model is presented for the quantitative prediction of the transfer of these contaminants to milk and multiple tissues of high-yielding dairy cows. The model is parametrized using a comprehensive in vivo study in which cows were orally exposed to 35 dioxin and PCB congeners. To describe the transfer to cow tissues, the wbPBTK model incorporates dynamic changes in the physiological characteristics across lactation and comprises 11 compartments, including blood, adipose tissues, liver and udder. Congener-specific parameters were derived from the data based on a novel modular Bayesian inference approach designed to handle the numerical challenges of this experimental dataset, such as the imbalance between numerous milk measurements and comparatively few tissue measurements. A final model was derived for 29 congeners, showing at least reasonable performance in describing milk data for 28, adipose tissue data during lactation for 27, blood data for 21, and organ tissue data at slaughter for 21 congeners. It includes an estimation of the prediction uncertainties using credible intervals. A code implementation for German Holstein cows is included, which may be modified for other breeds, ages or milk yields. The model is also available for interactive calculation in the ConTrans.bfr.bund.de platform tool to support exposure assessment and risk management for dioxins and PCBs in dairy production.

Copper (Cu) supplementation is essential in pig nutrition; however, its effects on performance, trace element accumulation in edible tissues, and environmental excretion require careful evaluation. In the present study a total of 24 male, castrated fattening pigs of two different hybrid mast lines (11 weeks of age) were divided according to their initial body weight (25.8 ± 3.5 kg) into four groups. Pigs were fed for 14 weeks a complete feed supplemented with Cu covering a range between the recommended Cu supplementation and the permissible European maximum level (i.e. 5, 15, and 25 mg/kg complete feed). Two different Cu sources were used: Cu sulphate (CuSO₄) and glycine-Cu chelate hydrate (Cu-Gly). The aim of the trial was studying the influence of the different Cu levels and sources on growth performance, Cu transfer to edible tissues (muscle, liver, kidney, blood) and faeces, as well as the concentration of other trace elements, including iron (Fe), zinc (Zn) and manganese (Mn) in tissues of fattening pigs. Performance parameters, including average daily gain (ADG) and feed conversion ratio (FCR), showed a dependency with respect to the pig breed, whereas Cu content and Cu sources showed no influence. Copper concentrations in the liver, kidney, muscle, and blood serum remained constant across groups. Faecal Cu excretion increased proportionally with dietary Cu levels, with higher excretion observed for the organic Cu source at 15 mg/kg as compared with inorganic source at similar level. The results show, that Cu levels up to the maximum level of 25 mg/kg complete feed are not necessary to achieve good growth performance in healthy fattening pigs. The unaffected Cu concentrations in liver, muscle, kidney, and blood, as well as increased faecal excretion with increasing Cu level in the feed, indicate an adequate supply and homoeostatic regulation of Cu. In addition, a reduced use of Cu in pig fattening will help to reduce Cu emission into the environment.

Tansy ragwort (Senecio jacobaea L.) growing in animal pasture may pose a risk to humans due to the potential transfer of pyrrolizidine alkaloids (PAs) into food of animal origin. Here, we investigated what amount of PAs corresponds to the amount of ragwort consumed by sheep on a pasture and whether the ingested PAs are transferred into edible tissue. From 2020 to 2021, a field study was conducted with 70 sheep grazing on a pasture (stocking density of 12 sheep/hectare) with considerable quantities of tansy ragwort. After slaughter, blood samples were taken for analysis of liver enzyme activities and haemoglobin concentration. Samples of ruminal fluid, liver and diaphragm pillar were analysed for PAs. The amount of ingested ragwort was determined by counting missing plant parts and calculating their weight using reference material. The mean daily intake of ragwort per sheep ranged from 0.16 kg to 4.89 kg fresh matter and corresponded to PA doses from 0.3 to 40.9 mg/kg body weight with no effect on the liver enzyme activities. The PA concentrations in the animal tissue were between the limit of detection and a maximum of 8.0 mu g/kg in the liver and 2.5 mu g/kg in muscle. These data suggest that the risk is negligible of exposure to PAs through consumption of meat or liver.

Per- and polyfluoroalkyl substances (PFAS) are a group of anthropogenic persistent pollutants, which are ubiquitously distributed in the environment and are linked to several adverse health effects. To monitor PFAS in the environment, wild boars (Sus scrofa ferus) have been proposed as bioindicator organisms, as their omnivorous diet can lead to intake of PFAS from their immediate environment. The study aimed to evaluate the influence of land use on the occurrence of PFAS in wild boar livers hunted in Brandenburg, Germany. For this purpose, 164 wild boars from 18 hunting districts were sampled and their livers were quantitatively analyzed for the presence of 16 PFAS. The hunting districts were characterized based on land use features using publicly available geodetic data. Additionally, geolocations of presumptive PFAS sources were used to evaluate the possible influence of their proximity to hunting districts on the PFAS burden. Statistical analyses revealed significant positive correlations between concentrations of PFOA, PFOS, PFNA, and PFDA and the proportional extent of urban and/or industrial areas. PFUnDA was statistically correlated with the extent of agricultural areas in the studied regions. Furthermore, the analyses indicated significantly higher concentrations of PFOA and PFNA in livers of wild boars hunted in districts located within 10 km from presumptive PFAS sources. In summary, the results demonstrate that PFAS concentrations in wild boar livers differ between hunting sites and indicate that land-use-based assessments are a feasible approach to locally characterize potential risks associated with the consumption of wild boar livers in terms of PFAS exposure.

Per- and polyfluoroalkyl substances (PFAS) constitute a group of anthropogenic compounds that are ubiquitously distributed in the environment due to their widespread use in various industrial processes and consumer products. Among foodstuffs of animal origin, wild boar (Sus scrofa) livers may contain particularly high levels of PFAS. Additionally, wild boars have been identified as suitable bioindicators and are used by German national authorities for monitoring purposes. In the context of consumer health risk assessment, information on influencing environmental factors is crucial for the risk analysis process. The objective of this study was to systematically analyze reports from the national monitoring program on PFAS concentrations in wild boar livers from 3 German federal states (n = 217). The analysis was based on publicly available data on land use characteristics, including urbanization, industry, agriculture, and forest areas in the sampled regions. Overall, data on sample characteristics (e.g., age, sex, body weight) varied between and within Federal States and counties. Despite these inconsistencies, statistical analyses revealed significant differences in the average PFAS concentrations between counties within the Federal States. Additionally, significant correlations were observed between land use features and PFAS concentrations, varying by Federal State and substance. In conclusion, land-use-based evaluations of food monitoring data may prove valuable for characterizing PFAS levels in wild boar liver. Harmonizing sampling protocols, data collection, and analytical methods among state laboratories will improve the comparability and interpretation of official monitoring data in future assessments.

The study examined the vitamin A status in commercially managed suckler cows and lactating dairy cows and identified primary influencing factors. Liver retinyl ester concentrations were higher in multiparous than primiparous cows (p < 0.01). Pasture availability was associated with higher β-carotene concentrations (p < 0.001). In dairy cows, pasture access during the dry period did not affect any of the parameters assayed. β-Carotene and retinol in milk increased with parity. No vitamin A deficiency or hypervitaminosis A was detected. Liver and milk retinol and retinyl ester concentrations that were analysed in the present study and data from a recent German total diet study were used to estimate the exposure to preformed vitamin A in vulnerable groups (children, 0.5–5 years). 95th percentiles of preformed vitamin A intake do not exceed tolerable upper intake levels in individuals between 1 year and 5 years, but in infants 6 to 12 months of age.

The transformation of hypoglycin A (HGA), hypoglycin B (HGB), and methylene cyclopropyl glycine (MCPrG) in ruminal fluid batch cultures was investigated, and the effect of these toxins on the batch culture microorganisms using microbial metabolites was measured. An experiment was conducted using ovine ruminal fluid batch cultures and the ANKOM RF Gas Production System over four runs, each with an incubation period of 48 h. The fermenters contained 200 mg of (i) a substrate mixture (80% cellulose, 20% starch; CSM), (ii) CSM and 1.5 mL of a solution of pure toxins (a mixture of 500 ng/mL HGA and MCPrG each; PCM), or (iii) CSM and 100 mg sycamore maple seeds (SMS). Each fermenter contained 30 mL of inoculum (ruminal fluid and buffer, 1:2 v/v). For control, autoclaved ruminal fluid was incubated with CSM, PCM, and SMS, respectively. Samples were taken from the liquid phase of the fermenters and analyzed using liquid chromatography–tandem mass spectrometry (LC/MS-MS) for sycamore maple toxins and metabolites. Microbial activity was assessed using gas production, short chain fatty acids, and NH3 concentration. Additionally, pH and redox potentials were measured. In PCM, HGA and MCPrG concentrations rapidly decreased (p < 0.05), and were not measurable anymore after a 24 h incubation period. In SMS, the initial concentrations were 4.7 ± 1.4 µg/mL HGA, 19.9 ± 5.41 µg/mL HGB, and 1.2 ± 0.33 µg/mL MCPrG. In SMS, HGA increased in 24 h, coincidently to a decrease in HGB concentration (p < 0.05). We modeled a rapid conversion of HGB to HGA, accompanied by progressive HGA transformation. The concentration of MCPrG was constant until 4 h and decreased afterwards (p < 0.05). In SMS incubations, HGA and MCPrG concentrations of 5.6 ± 1.5 and 0.32 ± 0.090 µg/mL remained after 48 h, respectively. The HGB to HGA conversion and transformation of HGA and MCPrG also occurred in autoclaved ruminal fluid. Gas production and microbial metabolite concentrations were higher in SMS compared to CSM and PCM (p < 0.05), as the seeds were used as an additional substrate by the batch culture microorganisms.

The workshop “Health Risks and Bioavailability of Metal Residues from Hunting Ammunition in Game Meat,” held in Billund, Denmark, explored the pressing issue of lead contamination in game meat. Experts from various disciplines discussed the health risks posed to humans and wildlife, the environmental impacts of lead residues, and potential alternatives to leadbased ammunition. Key findings highlighted the bioavailability and toxicity of lead from ammunition, the range and possibilities of current non-lead alternatives, and the need for harmonized regulations across Europe. The workshop underscored the importance of hunter education, public awareness, and interdisciplinary research to facilitate the transition to nontoxic ammunition and reduce contamination. Recommendations include stronger enforcement of lead bans, targeted outreach to stakeholders, and comprehensive risk assessments for alternative materials. These efforts aim to ensure the sustainability of hunting practices and the safety of game meat in the food chain.

Over the last years, African swine fever (ASF) has gone pandemic and within the European Union affected wild boar populations are main drivers. This brings new challenges, i.e. risk assessment needs for agricultural products and the role of mechanical arthropod vectors. Answering the call “Survival of African swine fever virus in feed, bedding materials and mechanical vectors and their potential role in virus transmission” (GP/EFSA/ALPHA/2021/09), relevant feed and bedding materials were chosen for stability experiments. All matrices were contaminated with ASFV and stored at five different ambient conditions (-20°C, 4°C, 10°C, 20°C, and 37°C) over a period of up to nine months. Replicate samples were evaluated at different time-points using real-time PCRs and virus isolation. Additionally, the possible role of three types of blood-sucking arthropods was assessed. In detail, studies were carried out on how long representative arthropods harbored viral genome and infectious virus upon feeding on infectious blood. In a last step, further proof-of-concept data were generated on the transmission of ASFV via ingestion of (small) arthropods after an infected blood meal. Concluding, detection of infectious virus was rather limited in most matrices while detection of viral genome was possible over the entire study period. At lower temperatures, however, the virus was stable on feed matrices over several days or even weeks, especially on beet and potatoes. Grass, grass silage and corn silage did not allow re-isolation of virus at any time-point. The studies on the detectability of the virus in arthropods showed that the virus is generally detectable for a certain period of time depending on temperature and ingested volume. The detectability of virus in stable flies exceeded the expectations with over 168 hours at cool temperatures. The feeding experiment did not lead to infection of pigs. However, the power of this proof-of-concept study is limited.

Little is known about knowledge and perceptions of the public and farmers on livestock feed. However, it is important to know their perspectives to find widely accepted and sustainable solutions in agriculture, which account for animal welfare, societal expectations, economy and the environment alike. Therefore, the objective of the present study was to assess knowledge, perceptions and informational needs regarding livestock feed among the general population and farmers. A German-wide online survey was conducted with n = 1000 participants from the general population (representative for age and gender) and n = 251 farmers. Differences in answers were compared between the general population and farmers as well as between subgroups of the general population. Results indicate that the public is correctly informed about some livestock feeds, although knowledge gaps and misconceptions became evident. The general population rated potatoes, fodder beets, kitchen waste and bread as common feedstuffs for pigs, which was rather a common practice in smallholder “backyard” pig husbandry several decades ago. Ratings of relevant aspects of feed differ between the two groups and partially depend on sociodemographic variables (i.e. gender, rural/urban upbringing, age) in the general population. Farmers were more likely to have heard and know the meaning of the term feed additives and are better informed about the functions that are fulfilled by feed additives. Farmers also expressed higher agreement for use of most alternative feeds than participants from the general population, although no differences of acceptance levels were found for algae, insects, animal by-products and fungi. In the group of the general population, 56% agree with the use of cereals and only 17% with the use of soy as livestock feed. When asked for the level of knowledge on livestock feed, 42% of participants from the general population indicate low or very low knowledge, whereas 97% of farmers judged the knowledge among the public to be low or very low. Both groups rate the need for information on controls of feed highest. Providing more information on livestock feed to the public seems necessary to improve knowledge, increase acceptance of alternative feeds and of sustainable solutions in agriculture.

Hemp (Cannabis sativa L.) is an important source of fibre and seed oil and protein. By-products of industrial hemp fibre production, like hemp seeds and cakes, can be used as feed for all animal species as fat and protein source and the whole hemp plant (including stalk and leaves) might be a suitable fibre source for ruminants. However, a previous feeding experiment with leaf-flower-seed hemp silage, made from an industrial hemp variety, demonstrated detrimental effects on cow health parameters and a significant transfer of several cannabinoids, including the psychoactive tetrahydrocannabinol (triangle 9-THC), into cow's milk, posing a potential risk to the safety of consumers. Based on those observations, the present study tested the hypothesis that anaerobic fermentation, as during ensiling, increases the content of triangle 9-THC in hemp. Therefore, silages of whole plants from the industrial hemp Cannabis sativa L. var. Ivory were prepared in a multifactorial design, with the four treatments 1) untreated control (CON), 2) addition of 10 mL per kg fresh weight homofermentative lactobacilli at 105 cfu/mL (LBAC), 3) addition of 10 mL per kg fresh weight homofermentative lactobacilli at 105 cfu/mL plus 30 g molasses (LBACmol) and 4) addition of propionic acid (10 mL/kg fresh weight) (PRO). Ultra high performance liquid chromatography coupled with tandem mass spectrometry with electrospray ionisation (UHPLC-MS/MS) was performed for analysis of cannabinoids in fresh hemp material and after 10 and 90 days of ensiling. The study revealed that ensiling decreased all acid forms of analysed cannabinoids in hemp at about 40-65% of the initial values after 90 days of storage, with the exception of cannabinolic acid (CBNA), and CBGA, the acidic form of cannabigerol (CBG). This decrease in most acidic forms was accompanied by an increase of the corresponding non-acidic forms of all cannabinoids, including the psychoactive triangle 9-THC. Thus, although ensiling decreases the total cannabinoid content, psychoactive compounds like triangle 9-THC can increase, enhancing the risk for animal health and a transfer of these substances into animal derived products. Industrial hemp can be ensiled with different additives, despite its high buffering capacityUltra high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used for analysis of cannabinoidsEnsiling decreased total cannabinoid content in industrial hemp, but increased individual compounds like triangle 9-THC, likely through decarboxylation of the precursor triangle 9-THCA.

The microbiome of wild animals is believed to be co-evolved with host species, which may play an important role in host physiology. It has been hypothesized that the rigorous hygienic practices in combination with antibiotics and diets with simplified formulas used in the modern swine industry may negatively affect the establishment and development of the gut microbiome. In this study, we evaluated the fecal microbiome of 90 domestic pigs sampled from nine farms in Canada and 39 wild pigs sampled from three different locations on two continents (North America and Europe) using 16S rRNA gene amplicon sequencing. Surprisingly, the gut microbiome in domestic pigs exhibited higher alpha-diversity indices than wild pigs (P < 0.0001). The wild pig microbiome showed a lower Firmicutes-to-Bacteroidetes ratio and a higher presence of bacterial phyla Elusimicrobiota, Verrucomicrobiota, Cyanobacteria, and Fibrobacterota when compared to their domestic counterparts. At the genus level, the wild pig microbiome had enriched genera that were known for fiber degradation and short-chain fatty acid production. Interestingly, the phylum Fusobacteriota was only observed in domestic pigs. We identified 31 ASVs that were commonly found in the pig gut microbiome, regardless of host sources, which could be recognized as members of the core gut microbiome. Interestingly, we found five ASVs missing in domestic pigs that were prevalent in wild ones, whereas domestic pigs harbored 59 ASVs that were completely absent in wild pigs. The present study sheds light on the impact of domestication on the pig gut microbiome, including the gain of new genera, which might provide the basis to identify novel targets to manipulate the pig gut microbiome for improved health. IMPORTANCE:The microbiome of pigs plays a crucial role in shaping host physiology and health. This study sought to identify if domestication and current rearing practices have resulted in a loss of co-evolved bacterial species by comparing the microbiome of wild boar and conventionally raised pigs. It provides a comparison of domestic and wild pigs with the largest sample sizes and is the first to examine wild boars from multiple sites and continents. We were able to identify core microbiome members that were shared between wild and domestic populations, and on the contrary to expectation, few microbes were identified to be lost from wild boar. Nevertheless, the microbiome of wild boars had a lower abundance of important pathogenic genera and was distinct from domestic pigs. The differences in the microbial composition may identify an opportunity to shift the microbial community of domestic pigs towards that of wild boar with the intent to reduce pathogen load.

Background: Methionine (Met) is a popular nutritional supplement in humans and animals. It is routinely supplemented to pigs as L-Met, DL-Met, or DL-2-hydroxy-4-(methylthio) butanoic acid (DL-HMTBA). Methods: We investigated the effect of these Met supplements on jejunal amino acid (AA) transport in male castrated Piétrain × Danbred pigs, also including a non-supplemented group. The mucosal-to-serosal flux of ten [14C]-labeled AAs (L-glutamine, glycine, L-leucine, L-lysine, L-Met, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine) was investigated at two concentrations (50 µM and 5 mM). Inhibition of apical uptake by mucosal L-Met was also measured for these AAs. The intestinal expression of apical AA transporters, angiotensin-converting enzyme II and inflammation-related genes were compared with those of a previous study. Results: Except for tryptophan and lysine at 5 mM, all AA fluxes were Na+-dependent (p ≤ 0.05), and the uptake of most AAs, except glycine and lysine, was inhibited by L-Met (p < 0.001). A correlation network existed between Na+-dependent fluxes of most AAs (except tryptophan and partly glycine). We observed the upregulation of B0AT1 (SLC6A19) (p < 0.001), the downregulation of ATB0,+ (SLC6A14) (p < 0.001) and a lower expression of CASP1, IL1β, IL8, TGFβ and TNFα in the present vs. the previous study (p < 0.001). Conclusions: The correlating AAs likely share the same Na+-dependent transporter(s). A varying effect of the Met supplement type on AA transport in the two studies might be related to a different level of supplementation or a different inflammatory status of the small intestine.

Hypoglycin A (HGA), methylenecyclopropylglycine (MCPrG), hypoglycin B (HGB), and γ-glutamyl-α-(methylenecyclopropyl) glycine (γ-glutamyl-MCPrG) are secondary plant metabolites occurring in sycamore maple (Acer pseudoplatanus) as well as several other Sapindaceae (e.g., Blighia sapida). By interfering with energy metabolism, they may cause severe intoxication in humans and other species. However, to date, there is not enough data available concerning the intake, metabolism, or excretion of sycamore maple toxins in dairy cows. In May 2022, five cows were observed over four days, when they had first access to a pasture with two sycamore maples. Grazing of their seedlings that grew numerously in between the pasture plants was monitored by direct observation. Milk samples were drawn both from individual cows and from the bulk tank. Spontaneous urine samples were collected from all cows on day 3 after access to the pasture. Seedlings (100 g) were sampled on the pasture and analyzed, together with milk and urine samples, for sycamore toxins and their metabolites using liquid chromatography-tandem mass spectrometry and liquid chromatography-high-resolution mass spectrometry. Cows ingested sycamore seedlings while grazing. Values of HGA in milk were below the limit of quantification. However, metabolites of HGA and MCPrG were detected in individual milk samples already at the end of the first day of grazing. Urine samples of all five cows showed higher concentrations of conjugated HGA and MCPrG metabolites than in milk. Observations suggest that dairy cows may have a low susceptibility toward sycamore maple toxins. However, whether this could be attributed to foregut fermenting species in general requires further elucidation.

Zusammenfassung Die ganzheitliche Betrachtung der Lebens- und Futtermittelsicherheit unter Einbeziehung der Tiergesundheit und der Umweltbedingungen ist eine wichtige Säule des One-Health-Ansatzes. Die Begrifflichkeit geht damit deutlich über die häufig darunter verstandene Vermeidung der Verbreitung von übertragbaren mikrobiologischen Krankheiten hinaus und verdeutlicht, dass Mensch, Tiere und Umwelt sowie ihre Interaktionen in einem transdisziplinären Kontext betrachtet werden sollten. In dem vorliegenden Diskussionsbeitrag zum One-Health-Ansatz liegt der Fokus weniger auf mikrobiologischen Risiken, sondern es wird insbesondere der Zusammenhang zu chemischen Risiken in der Nahrungskette hergestellt. Dieser wird an konkreten Beispielen chemischer Kontaminanten (Metalle, persistente organische Kontaminanten und natürliche Toxine) verdeutlicht und die Mechanismen von Eintrag und Weitergabe entlang der Nahrungsketten werden vorgestellt. Die Minimierung des Vorkommens chemischer Kontaminanten und somit der Exposition erfordert internationale und interdisziplinäre Zusammenarbeit. Klimawandel, Pandemien, Rohstoffknappheit, Energiemangel, politische Krisen und Umweltkatastrophen können die gesamte Kette der Primärproduktion pflanzlicher und tierischer Lebensmittel sowie die Weiterverarbeitung und Bereitstellung der Produkte für Verbraucherinnen und Verbraucher beeinträchtigen. Neben der sich verändernden Verfügbarkeit kann dies auch einen Einfluss auf die Zusammensetzung, Qualität und Sicherheit der Lebens- und Futtermittel haben. Anhand der Effekte auf globalen Warenketten werden vulnerable und resiliente Bereiche sichtbar. Im Sinne des One-Health-Ansatzes gilt es, die Sicherheit und Resilienz entlang der Nahrungskette zu erhöhen und deren Vulnerabilität zu minimieren.

The German Total Diet Study (BfR MEAL Study) measured copper in 356 foods. In 105 of these foods copper was determined separately for conventionally and organically pooled samples. Mammalian liver, nuts, oilseeds, cocoa powder and chia seeds contained the highest copper levels. Organically produced foods tended to have higher levels compared to conventionally produced foods. Children's copper exposure was between 0.04 mg/kg body weight per day (mg/kg bw/day) and 0.07 mg/kg bw/day (median). High exposure (95th percentile) ranged between 0.07 mg/kg bw/day and 0.11 mg/kg bw/day. Adult's exposure ranged between 0.02 mg/kg bw/day (median) and 0.04 mg/kg bw/day (95th percentile). Grains and grain-based products were main contributors for all age groups. Copper intake was about 10% higher in a scenario where consumers select the organically produced variants. Children's median and high exposure was above the acceptable daily intake (ADI) of 0.07 mg/kg bw/day set by the European Food Safety Authority (EFSA). However, according to EFSA's evaluation this is not of concern due to higher requirement related to growth. For adults, frequent consumers of mammalian liver exceeded the ADI in median and 95th percentile. Intake of copper-containing dietary supplements may also lead to exceedance of the ADI in all age groups.