OBJECTIVE:To demonstrate an underutilized, reliable, and technically simple anuran venipuncture technique by use of the popliteal sinus. ANIMALS:Medium to large (> 30 g) anuran species. METHODS:The animal is manually restrained using an encircling grip around the pelvic girdle. With the ventrum facing the phlebotomist, the phlebotomist grasps the distal hind limb and gently extends it. If necessary, the medial stifle can be rinsed with sterile water or saline to remove gross debris. Using a caudomedial approach, a 25- to 27-G, 0.5-inch (1.27-cm) needle and attached syringe is advanced toward the stifle at an approximately 45° angle while maintaining negative pressure. If lymphatic fluid or a visibly lymphatic fluid-contaminated blood sample is obtained, the sample is discarded and the attempt is repeated with a new needle and syringe. Collection volume should not exceed 1% of body weight in systemically healthy animals. In compromised animals, a reduction in collection volume should be considered (eg, ≤ 0.5% of body weight). While the phlebotomist may have an ergonomic preference, either hind limb can be used for blood collection. RESULTS:Popliteal sinus venipuncture in anurans can be safely and easily performed with only manual restraint. CLINICAL RELEVANCE:Commonly utilized anuran blood collection techniques include venipuncture of the ventral abdominal vein and lingual venous plexus and cardiocentesis. These techniques can be technically difficult and may pose risks to the patient, particularly if attempted without chemical restraint. Popliteal sinus venipuncture is a reliable, technically simple alternative blood collection technique that can be safely performed in anurans with only manual restraint.

The parakeet auklet (Aethia psittacula) is a piscivorous seabird with a natural diet of various invertebrate and teleost species, which is challenging to replicate in a managed collection. A high prevalence of early onset cataracts was observed in a managed collection of parakeet auklets at the North Carolina Zoo (Asheboro, NC, USA), which was hypothesized to be related to inappropriate vitamin A and E levels. From 1994 to 2002, these parakeet auklets were offered dietary supplementation comprising Vita-Zu small bird tablets. In June 2002, the birds were transitioned to only Thiamin-E paste (vitamin E and thiamin only). Plasma samples were collected from birds with and without cataracts from 1998 to 2005 and submitted for vitamin A (retinol) and vitamin E (α-tocopherol) analysis. Food items comprising the birds' diet were also evaluated for vitamin content. This information was combined with clinical and necropsy data from medical records from 1994 to 2015. A total of 78% of birds (39/50) developed cataracts, with a median age of onset of 7 years (range, 2-12 years). Cataracts ranged from incipient to hypermature during both routine ophthalmic examinations and postmortem evaluations. The median (range) of plasma retinol and α-tocopherol values were 1.99 µg/mL (0.20-6.68 µg/mL) and 15.39 µg/mL (3.40-96.27 µg/mL), respectively. There were no significant differences in plasma concentrations of vitamins based on the animals' sex, origin, presence of cataracts, or administered vitamin supplementation product. No other etiologies for cataract development were identified in the population. Further research in free-ranging parakeet auklet nutrition and cataract occurrence is warranted for continued species collection management.

CASE DESCRIPTION 4 wild adult rat snakes ( Pantherophis alleghaniensis ) were evaluated after ingesting spherical or ovoid foreign bodies. CLINICAL FINDINGS Physical examination revealed a large, firm mass at the level of the stomach in each snake. Radiographic findings were consistent with ingestion of a golf ball (3 snakes) or an artificial egg (1 snake). Signs consistent with prolonged impaction included scale loss, dermal abrasions, and apparent loss of body condition in one snake and regional skin ulceration, dehydration, and generalized muscle atrophy in another. TREATMENT AND OUTCOME Nonsurgical removal of the foreign body was attempted in anesthetized or heavily sedated snakes by external manipulation in the orad direction. A golf ball was removed through the oral cavity without complications in 1 snake. In the other 3 snakes, tension caused by the advancing foreign body resulted in full-thickness skin rupture in the cervical region. The procedure was completed with the use of a balloon catheter to aid foreign body advancement for 1 of the 3 snakes, and the skin defect was closed. The procedure was converted to esophagotomy for the other 2 snakes. Three snakes recovered and were released; 1 died of complications from prolonged impaction and esophageal perforation. CLINICAL RELEVANCE The described nonsurgical techniques for removal of ingested round or ovoid foreign bodies were associated with substantial complications in 3 of 4 treated rat snakes. Although a nonsurgical method for removal of ingested objects such as golf balls could benefit snakes, the methods used for these patients did not appear to be more beneficial than traditional gastrotomy.

A wild, adult, female Florida cooter (Pseudemys floridana floridana) was evaluated for injuries sustained after vehicular trauma. On presentation the turtle had a minimally displaced caudal carapacial fracture, marked hyphema in the right eye, epistaxis, and frank hemorrhage from the cloaca. Immediately following initial triage, the turtle laid eight eggs and produced seven more over 72 h. After approximately 3 wk of hospitalization and treatment, the turtle received access to an outdoor water enclosure. Positive buoyancy was observed and a large, gas-filled, Y-shaped structure was identified in the caudal coelom via computed tomography. Cloacoscopy confirmed right-sided accessory cystitis, secondary to a focal necrotic wound with a mixed bacterial infection. After wound debridement and 3 wk of antibiotic therapy, the patient successfully submerged, there was no gas present in the accessory bladders, and the previous necrotic wound showed excellent healing on recheck cloacoscopy. This is the first report of accessory cystitis in the literature and it should be considered a differential for positive buoyancy in chelonian species that possess accessory bladders.

A 37-year-old bull African elephant (Loxodonta africana) at the North Carolina Zoo (NCZ) was diagnosed with bilateral cataracts leading to behavioral changes and significant weight loss secondary to functional blindness. On initial examination, a weight loss of 234 kg, a mature cataract in the right eye, and a focal cataract in the left eye were diagnosed. Ultrasound and electroretinography (ERG) indicated normal retinal attachment and both eyes were viable candidates for surgery. After careful planning and behavioral training, the left cataract was surgically removed via phacoemulsification and irrigation/aspiration. The right eye subsequently developed a ventral lens subluxation, and phacoemulsification and irrigation/aspiration were performed six months after the first procedure. Four years after surgery, menace response, palpebral reflex, dazzle reflex, and pupillary light reflexes were present in both eyes. Body weight was 5,515 kg, 88kg more than at the time of the second surgery. This is the first published report of an African bull elephant undergoing bilateral cataract removal using phacoemulsification and irrigation/aspiration. The lack of significant postoperative inflammation and uneventful recovery of the elephant suggests that this surgical procedure along with proper preoperative planning and postoperative medical management can be a safe and effective treatment option for elephants with cataracts.

Three sexually mature female common vampire bats (Desmodus rotundus) housed at the North Carolina Zoological Park, Asheboro, North Carolina, were selected for surgical ovariohysterectomy. All animals were induced and maintained with isoflurane anesthetic gas. Magnification loop glasses were worn by the surgeon for the procedure. A ventral midline incision was made into the abdominal cavity. Simple micro-ophthalmic surgical packs along with hemoclips were used to perform the ovariohysterectomies. The linea alba and muscular layers were closed using a simple continuous suture pattern with 4-0 polydioxanone suture. The skin was apposed using a horizontal mattress suture pattern with 4-0 polydioxanone suture. Animals recovered with minimal deleterious side effects. Animals were housed together in a recovery chamber and administered meloxicam at 0.2 mg/kg placed in their blood meal once daily for 7 days postoperatively, after which they were returned to their normal enclosures.

OBJECTIVE The objective of this study was to compare an implanted direct blood pressure monitor and a non-invasive oscillometric unit for use in anesthetized and awake green iguanas. STUDY DESIGN Prospective experimental trial. ANIMALS Four male and four female adult green iguanas (Iguana iguana) weighing 1833 +/- 534 g. METHODS For each animal, the carotid artery was surgically exposed and the catheter tip of the pressure transducer was placed in the aortic arch. Non-invasive blood pressure was measured using a cuff over the left femoral region. Pulse rate, respiratory rate and arterial blood pressure (ABP) measurements were taken every 5 minutes. Direct ABP measurements consisted of recording numerical values and graphic output. Simultaneous direct and indirect measurements were repeated in awake animals. RESULTS The oscillometric device failed to provide a reading in over 80% of attempts, and failed to provide readings that correlated with direct measurements. The implanted direct transducer was capable of detecting blood pressures throughout all ABP ranges examined. CONCLUSIONS The implantable transducer was a reliable means of determining blood pressure in this study, while the oscillometric device was unreliable and often failed to provide any reading. CLINICAL RELEVANCE We do not recommend using the oscillometric device as described in a research or clinical setting for green iguanas. The advantages of an implantable device include the ability to monitor awake and anesthetized subjects remotely and continuously. These monitors are small, biocompatible and function across a wide range of ABP.

A case of cerebral xanthomatosis is described in a long-nosed snake (Rhinocheilus lecontei). A 13-year-old long-nosed snake presented for pronounced lethargy, anorexia, and diminished righting reflex. The snake failed to respond to medical therapy and was euthanized. On histology, a focal cerebral cholesterol granuloma (xanthoma) was found within the lateral ventricle causing mild hydrocephalus, marked compression atrophy of the overlying dorsal cortex, and mild compression and disorganization of the ventral nucleus sphericus. Xanthomatosis is believed to be the cause of the neurological signs and the ultimate lack of response to medical therapy in this case. This case demonstrates another differential for neurologic dysfunction in snakes.

OBJECTIVE:To determine the accuracy of a noninvasive oscillometric monitor in the measurement of arterial blood pressure in anesthetized boid snakes.DESIGN:Evaluation study.ANIMALS:4 boa constrictors (Boa constrictor), 2 carpet pythons (Morelia spilota), and 2 reticulated pythons (Python reticulatus).PROCEDURES:After induction of anesthesia with isoflurane, each snake was instrumented with an arterial catheter connected to a pressure transducer and oscilloscope to obtain invasive measurements of systolic (SAP), diastolic (DAP), and mean (MAP) arterial blood pressure as well as a pressure waveform. A cuff connected to an oscillometric device was placed on the tail immediately distal to the vent for noninvasive measurements. Heart rate, respiratory rate, and invasive and noninvasive measurements of SAP, DAP, and MAP were obtained every 5 minutes for 45 minutes. Delivered isoflurane concentration was increased in 15-minute increments to induce hypotension. Repeatability of each device and fixed and proportional biases between devices were calculated.RESULTS:Throughout most of the measured ranges of blood pressures, the oscillometric unit overestimated the SAP and underestimated the DAP and MAP, compared with respective direct measurements. When the invasively determined SAP was > 100 mm Hg, the oscillometric unit underestimated all 3 variables. Fixed bias was significant for SAP and DAP, and proportional bias was significant for SAP and MAP.CONCLUSIONS AND CLINICAL RELEVANCE:When using an oscillometric blood pressure monitor on anesthetized boid snakes, veterinarians can potentially monitor changes in blood pressure, although the displayed readings may underestimate DAP and MAP and overestimate SAP. Indirect measurements of blood pressure made with the oscillometric device cannot substitute for direct measurements.

An adult, wild-collected, male harp seal (Phoca groenlandica) was transferred from a rehabilitation center to a display facility because of unilateral phthisis bulbi and decreased use of the right forelimb, which precluded its release. In quarantine, the animal demonstrated limited use of the right forelimb, which acutely progressed to complete disuse of the limb accompanied by intermittent lethargy. One month after transfer, the animal was found dead on exhibit. Necropsy showed septic arthritis of the right scapulohumeral joint, valvular endocarditis with systemic bacterial thromboembolism, and infarction of the cerebrum and myocardium. Culture of the blood and affected joint space revealed Staphylococcus aureus. Bacterial polymerase chain reaction of formalin-fixed tissues from the heart and brain were also positive for S. aureus. Staphylococcus aureus infection should be considered as an additional cause of endocarditis and embolic encephalitis in seals.

Zoological medicine furthers the health and wellbeing of captive and free-ranging wild animals. Effective information retrieval of the zoological medicine literature demands searching multiple databases, conference proceedings, and organization websites using a wide variety of keywords and controlled vocabulary. Veterinarians, residents, students, and the librarians who serve them must have patience for multiple search iterations to capture the majority of the available knowledge. The complexities of thorough literature searches are more difficult for nondomestic animal clinical cases and research reviews as demonstrated by three search requests involving poisonous snakes, a gorilla, and spiders. Expanding and better disseminating the knowledgebase of zoological medicine will make veterinary searching easier.

An extracapsular stabilization technique was used to repair cruciate ligament ruptures in a trumpeter hornbill (Bycanistes bucinator) and an African grey parrot (Psittacus erithacus). The hornbill demonstrated cranial drawer motion and severe rotational instability of the stifle from ruptures of the cranial and caudal cruciate ligaments and stifle joint capsule. The luxation was reduced, and the fibula was cranially transposed, in relation to the tibiotarsus, and anchored with 2 positive profile threaded acrylic pins. A lateral extracapsular stabilization was then performed. The African grey parrot had a traumatic stifle luxation, and an open reduction and a lateral extracapsular stabilization were performed. Both birds regained function of the affected leg by 1 month after surgery. Extracapsular stabilization allows motion of the stifle joint to be maintained during the postoperative recovery period, an advantage over rigid stabilization. Maintaining motion in the stifle joint facilitates physical therapy and can aid in full recovery after avian stifle injuries.

Bacterial, fungal, and parasitic diseases in reptiles are occasionally caused by primary pathogens, but often are the result of an immunocompromising condition, such as inappropriate temperatures, humidity, or enclosure hygiene. Treating bacterial and fungal diseases usually requires addressing the predisposing husbandry deficiency. Recent comprehensive publications list many reported bacterial, fungal, and parasitic pathogens. This article discusses general methods for diagnosing and treating infectious diseases, and discusses certain diseases in relation to body systems. Special attention is given to recently reported diseases.

ABSTRACT This case report describes colonic impaction with rodent hair and associated transcolonic membrane formation in a Savannah monitor (Varanus exanthematicus). Medical management with repeated warm soapy water enemas and oral laxatives was unsuccessful. Contrast radiographs and colonic endoscopy showed a complete membranous obstruction of the distal colon. Removal of the membrane was not possible endoscopically, therefore an exploratory celiotomy and a colotomy were performed to remove the membrane. Postoperatively, the animal was managed with ceftazidime, penicillin, and neomycin for antibiotic therapy, morphine and meloxicam for pain management, and lactulose as a laxative. The diet was changed to skinned adult mice once weekly, with neonatal mice containing oral medications force-fed daily. Twenty-four days after the surgery, the patient was reevaluated and was not passing feces. A second surgery revealed recurrence of the membrane and a dilated, atonic proximal colon; euthanasia was elected. In ...

ABSTRACT Cystic calculi were detected during physical examination of an adult San Esteban Island chuckwalla, Sauromalus varius. This lizard had developed calculi on five previous occasions, requiring multiple surgical interventions. Cystectomy was elected to resolve the current episode and to prevent any additional calculi from forming. Plasma osmolarity and biochemistry analyses were evaluated at regular intervals before and after removing the bladder. No adverse clinical or biochemical effects were observed. Cystectomy is a viable option for managing chronic urolithiasis in captive reptiles, however, because the bladder can be a site of post-renal modification of urine, it may not be appropriate in certain species or situations.

A 16-yr-old, captive-born, female California sea lion (Zalophus californianus) was evaluated for intermittent lethargy, partial anorexia, and polydipsia of 2 wk duration. The animal was immobilized for physical examination. It was in thin body condition, with multifocal mucosal ulcerations over the caudal and ventral tongue. Blood was collected for hematology, serum chemistry, and leptospirosis serology. Serum chemistry revealed severe azotemia, mild hyperglycemia, and severe hyperphosphatemia. The animal went into cardiac arrest during recovery from anesthesia and died. On histopathology, abundant amorphous, finely fibrillar, eosinophilic material was deposited in the kidneys, and smaller amounts of the same material were found in the splenic and pancreatic vessels; these findings are consistent with systemic secondary amyloidosis. The animal also had chronic nephritis, which, coupled with renal amyloidosis, resulted in renal failure and death. Systemic amyloidosis should be considered as an additional differential diagnosis for renal failure in California sea lions.

ABSTRACT A presumably captive-born, 3-year old, female large day gecko, Phelsuma madagascariensis grandis, presented with a subcutaneous mass that contained a nematode of the subfamily Spendidofilariinae. Eleven months later the gecko presented for anorexia and depression 28 d post egg-laying. Radiographs showing two poorly calcified eggs were suggestive of dystocia and an exploratory laparotomy was performed. Severe, non-septic coelomitis associated with nine nematodes in granulomatous capsules and a retained egg were discovered. All nematodes were found to be in the subfamily Splendidofilariinae that have not been reported previously in day geckos.