In developing countries, fermentation is one of the traditional food processing methods for production of relatively safe and nutritious foods. Among many fermented foods in Ethiopia, "Shameta" is one of the locally produced and consumed cereal-based fermented porridge mainly used to support strength and recovery of lactating women after birth. However, even though the product is consumed for years, so far, the nutritional composition and bioactive compounds of the porridge not yet scientifically determined. This study aimed to determine the physicochemical properties, nutritional composition and bioactive compounds of "Shameta" commonly produced and consumed in Western part of the country. A total of 27 "Shameta" samples were collected from houses of lactating mothers residing in different districts of East Wollega zone. Results showed that, "Shameta" sample prepared from blend of maize and barely supplemented with faba bean results in crude protein content of 11.2 g/100g as compared with samples without faba bean, 6.8 g/100g. Samples supplemented with more proportion of rapeseed as oil source resulted in relatively higher crude fat content (12.2 g/100 g) as compared to other samples. From energy point of view, "Shameta" could provide about 85% of the extra energy needs of lactating mothers as compared to staple foods consumed in sample collection areas. It is also confirmed that, the product is a good source of iron and zinc, with the highest scores of 8.1 and 8.6 mg/100g in some samples, respectively, as compared to other mineral elements whose scores were much less than the daily recommended allowances. The average phytate and tannin contents were 0.79 and 0.18 mg/100g, respectively. Even though the Ca, Fe and Zn contents were below the recommended daily allowance, their bioavailability could not be hindered by phytate and tannin. Results also showed that samples have good antioxidant potential to minimize oxidative stresses. It could be deduced that as a sole food for the mothers, the product could not provide sufficient protein and some minerals to meet recommended daily allowance. However, to enhance the importance of the product, it is necessary to optimize the ingredient compositions and processing conditions to meet the nutrient demand of lactating mothers.

Infections with coronaviruses remain a burden that is negatively affecting human life. The use of metal oxides to prevent and control the spread of severe acute respiratory syndrome coronavirus (SARS-CoV-2) has been widely studied. However, the use of metal oxides in masks to enhance the performances of barrier face coverings in trapping and neutralizing SARS-CoV-2 remained unexplored. In the present study, we explore the possibility of developing surface functional PVA/ZnO electrospun nanowebs to be used as a component of multilayer barrier face coverings. Polyvinyl alcohol (PVA) and zinc acetate (ZnA) nanowebs were electrospun as precursor samples. After calcination at 400 degrees centigrade under a controlled atmosphere of nitrogen gas, product nanowebs containing ZnO (PVA/ZnO) were obtained. The presence of ZnO was determined using an attenuated total reflectance Fourier Transform Infrared (FT-IR) spectrometer. This study inspired the possibility of developing surface-functional materials to produce enhanced performance masks against the spread of SARS-CoV-2.

Glia cells provide supportive functions to the central nervous system and can be compromised by environmental contaminants. The primary objective of this study was to characterize the effects of in vitro exposure to perfluorooctanoic acid, a persistent environmental contaminant and/or monocrotophos (MCP), a neurotoxic organophosphate that is rapidly metabolized, to astroglia SVG p12 cells. The endpoints evaluated include cell viability, intracellular glutamate levels as a marker of astrocyte homeostasis function, differential gene expression for selected proteins, which include inflammatory markers (tachykinin), astrocytosis (nestin), S100B, and metabolism enzymes (CYP1A1). The results from cell viability revealed significant differences from the controls at some of the concentrations tested. Also, intracellular glutamate levels were elevated at the 10-μM concentration for perfluorooctanoic acid (PFOA) as well as the 10-μM PFOA/5-μM MCP concentration. Gene expression results at 80-μM PFOA concentration revealed a significant increase in the expression of S100B, tachykinin and CYP1A1. A combination of 10-μM PFOA/20-μM MCP caused a significant decrease in the expression of tachykinin. Gene expression for MCP exposures produced a decrease at the 20-μM MCP concentration. Immunofluorescence results indicated an increase in nestin protein expression for the 20-μM concentration of MCP, which contradicted the gene expression at the same concentration tested. The results indicate that toxicity to glia cells can compromise critical glia functions and could be implicated in neurodegenerative diseases.

Nanofiber substrates have been used for various biomedical applications, including tissue regeneration, drug delivery, and in-vitro cell culture. However, despite the high volume of studies in this field, current clinical applications remain minimal. Innovations for their applications continuously generate exciting prospects. In this review, we discuss some of these novel innovations and identify critical factors to consider before their adoption for biomedical applications.

Neurodegenerative diseases account for a significant portion of public health concerns particularly in the aging population. The dysfunction of interfilament proteins has been identified as a key event in the initiation of neurodegeneration and subsequent progression to neurodegenerative diseases. In addition, several studies have found associations between the dysfunction of interfilament proteins and exposure to environmental contaminants. Therefore, in this review, the role of interfilament proteins in neuronal cells, their connection to neurotoxicity from environmental contaminants, and finally the resulting neurodegeneration are discussed .

Membrane technology proved to be the most promising method for water treatment because of functionality, high efficiency, low cost and stability of smart membrane. In this study, novel effective PVDF/rGO/TiO2 nanofiber webs for oil/water separation are produced by incorporation of rGO/TiO2 nanoparticles in the electrospun solution. GO is prepared by Brodie's method while rGO/TiO2 is synthesized by the hydrothermal method. The morphology of PVDF/rGO/TiO2 nanofiber is tested by atomic force microscopy (AFM) and optical microscope. Moreover, the mechanical properties, water contact angle, fourier-transform infrared spectroscopy (FTIR), and oil-water separation property of these nanofiber webs are also tested. The results show PVDF/rGO/TiO2 nanofibers with rGO/TiO2 concentration of 3% have uniform diameter and diameter distribution. Additionally, the mean roughness (Ra) shows an increasing trend with rGO/TiO2 concentration from 0% to 20%. The mechanical properties of PVDF/rGO/TiO2 nanofiber webs are affected by the rGO/TiO2 concentration. FTIR results indicate the existence of GO and TiO2 peaks in the nanofiber webs. In addition, from the oil-water removal experiment, it can be observed that PVDF/rGO/TiO2 nanofiber webs with a rGO/TiO2 concentration of 3% has the highest oil removal efficiency at 98.46%. Overall, the hydrophobic PVDF/rGO/TiO2 nanofiber webs turned out to be promising materials for separating oil/water mixture.

A tensile properties testing study was conducted to understand the influence of thickness, cross-head speed (speed of testing), gauge length (GL; specimen test length), and sample shape on important tensile properties of polyvinyl alcohol (PVA) nanofiber webs. The effects of each testing parameter on load at break, extension at break, Young's modulus, and tensile stress-strain curve of PVA nanofiber webs are analyzed. The Welch two sample t-tests show the significant difference among tested data. Using interaction plots, two-way analysis of variance, and margin mean plots, the interaction effects among testing parameters have been analyzed. Of all the factors, cross-head speed, the interaction among GL, and sample thickness (GL: Thickness) and the interaction among GL, testing speed and sample thickness (GL: Speed: Thickness) have significant influence on the tensile properties of PVA nanofiber webs. Moreover, the hypothesized model of mechanism of tensile strain-stress curve of PVA nanofiber webs has been proposed. Based on the model, the tensile strain-stress curve can be split into three stages: linear elastic, partial break up, and complete breakage. This study will provide a better understanding of tensile testing parameters' effects and their interaction effects on the tensile properties of nanowebs.

Drug (vitamin B2, riboflavin, VB2)/nanoparticle (TiO2, coated on drug surface) loaded poly(vinyl alcohol) (PVA) nanofiber webs were successfully produced by the electrospinning process. The characteristics of nanofiber structure, chemical composition, mechanical properties, and drug-release properties were investigated. The morphology and diameter of nanofibers were analyzed by atomic force microscopy and scanning electron microscopy. The chemical composition and mechanical properties of nanofiber webs were examined by Fourier transform infrared spectroscopy and Instron tensile tester. The drug-release properties of nanofiber webs were studied by liquid chromatography-mass spectrometry (LC-MS/MS). The influences of TiO2:VB2 ratios (0, 18:1, 9:1, 4.5:1, 2.25:1, and 1:1) and releasing time on release behavior of PVA/VB2/TiO2 nanofiber webs were also investigated, with the corresponding virgin PVA nanofibers and LC-MS grade water as control. Among all of the tested samples, PVA/VB2/TiO2 nanofiber webs with TiO2:VB2 ratios of 18:1 and 9:1 exhibited a steady release rate with 60% of VB2 released around 168 h and all VB2 released around 10 days. In addition, by coating VB2 with TiO2, the burst release was effectively prevented due to three mechanisms: surface modification, polymer morphology, and coated surface. The results in this study indicate drug-loaded and nanoparticle-coated hydrophilic nanofiber webs are useful candidates for steady drug release in the drug delivery application field.

Developing baseline concentrations of serotonin in healthy white-tailed deer will allow for the development of a biomarker using non-invasive sample tissues in sick animals, for example, non-clinical cases of chronic wasting disease. It will also allow some further insight into whether the use of antibiotics as growth promoters (AGP), such as chlortetracycline, is affecting serotonin concentrations in white-tailed deer. Florfenicol and tulathromycin impacts on serotonin concentration changes were also investigated. An analytical method for the detection and confirmation of serotonin, 5-hydroxytryptamine (5-HT), in white-tailed deer tissues was developed and validated. Serum and urine samples were extracted with acetonitrile. Liquid chromatography separation was attained on a Phenomenex C18 column with a Security Guard ULTRA guard column with gradient elution using a mobile phase of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. This methodology was applied to baseline (control), chlortetracycline (CTC) treated, florfenicol treated and tulathromycin treated white-tailed deer serum and urine samples.

This chapter per the authors will introduce the reader to Complementary and Alternative Medicine (CAM) and shall discuss herbalism as a subset of CAM. Particular emphasis will be placed on herbal teas or rather infusions and decoctions used in disease therapy. This chapter will enumerate the different types of teas and shall use maps, graphs, and other tools to illustrate location, consumption, use and availability. Furthermore, the authors will highlight potential health benefits, recent studies (in vitro, in vivo) undertaken by research scientists to validate efficacy, and shall call for more research (clinical data management, clinical trials, etc.) and support for ongoing work in this area of expertise. The authors shall place a spotlight on the plant family, Asteraceae, and their herbal plants of interest, Artemisia annua and Brickellia cavanillesii. Extensive studies have been performed to determine the therapeutic potential of Brickellia cavanillesii plant at Ernest E. Smith laboratory, The Institute of Environmental and Human Health (TIEHH), Texas Tech University, Lubbock, USA.

We examined gonads and thyroid glands of Gulf killifish (Fundulus grandis) 1yr after the Deepwater Horizon oil spill. F. grandis were trapped from two impacted sites in Barataria Bay (Bayou St. Denis, Bay Jimmy) and an un-impacted site in East Texas (Sabine Pass). The greatest number of F. grandis were collected at Sabine Pass. F. grandis collected at Bayou St. Denis were smaller and had smaller Fulton condition factor scores than fish collected at Sabine Pass. Sex ratios were biased roughly 2:1 in favor of females at Sabine Pass and Bayou St. Denis. Gonad-somatic index (GSI) in males from Sabine Pass was double that of fish from Bay Jimmy while germinal epithelium thickness of the testes was 2.7 fold smaller in males from the impacted site. GSI and oocyte diameters in females from Bayou St. Denis were significantly smaller than females from Bay Jimmy or the reference site. There were no differences in thyroid follicle cell height. While total polyaromatic hydrocarbons at the impacted sites were no different from the reference site, the impacted sites did have greater concentrations of benzo[a]pyrene in sediment pore water. The finding of smaller GSI and testicular germinal epithelium in males from an impacted site suggest that exposure to a combination of oil and dispersants may adversely impact testicular function.

Electrospun poly(vinyl alcohol) (PVA) nanowebs treated with honey were prepared to exploit the high surface area of nanofibers and the many different beneficial properties of honey. Nanowebs fabricated from biocompatible polymers like PVA, and treated with natural substances like honey, may find use in various biomedical applications such as wound bandages. Fourier transform infrared spectroscopy was used to confirm the incorporation of honey into electrospun PVA nanowebs. Treated nanowebs were characterized by evaluating their antimicrobial properties, breathability characteristics, and tensile properties. PVA nanowebs treated with honey demonstrated adequate breathability characteristics for potential use in wound dressings. In this study, no antibacterial activity was observed after treatment with honey.

Electrospun polyvinyl alcohol (PVA) nanowebs treated with a mixture of honey and polyhexamethylene biguanides (PHMBs, commercially available as Reputex 20) were prepared and characterized to evaluate their applicability in wound dressing applications. Fourier transform infrared spectroscopy was used to confirm the incorporation of functional moieties from honey and Reputex 20 into electrospun PVA nanowebs. Functionalized PVA nanowebs were characterized by evaluating their antimicrobial properties, moisture vapor transport characteristics (breathability), and tensile properties. PVA nanowebs treated with a mixture of honey and PHMBs have shown good antimicrobial activity. Additionally, functionalized PVA nanowebs have shown adequate breathability characteristics, a much needed attribute in textile materials used in wound dressing applications. Nanowebs fabricated from bio-compatible polymers such as PVA, and functionalized in a combinational fashion, could be used in many different biomedical applications, including wound healing bandages and cell or tissue culture scaffolds.Application: The pulp and paper industry could use a similar combinational approach to develop multifunctional textiles for a variety of applications according to their needs.

Chlortetracycline is (CTC) is a tetracycline antibiotic which is being in the white-tailed deer industry to improve production and animal health. In this paper, we present a method for determining chlortetracycline residues in edible white-tailed deer tissues, using liquid chromatography with heated electrospray ionization and mass spectrometry detection. The procedure involved extraction with EDTA-McIlvaine buffer at pH 4.0, followed by solid-phase extraction cleanup using a hydrophilic-lipophilic balance (HLB) cartridge. The liquid chromatography analysis was performed with heated electrospray ionization and mass spectrometry detection. The limit of quantification for the method was 2.7 ng/g and limit of detection was 0.8 ng/g. The recovery values were > 78.5% for muscle, 65.1% for kidney, 63.1% for liver. Mean tissue residue concentration of chlortetracycline and it's epimer, 4-epi chlortetracycline (4-epi-CTC) at 10-day withdrawal period for kidney, liver, muscle was 122.8, 44.7 and 26.7 ng/g, respectively. Chlortetracycline tissue residue concentration at 45-day withdrawal period for kidney, liver, muscle was 19.2, 28.9 and 10.7 ng/g, respectively. Mean tissue concentration of CTC was less than the established maximum residual limit (MRL) values for bovine tissues. We have validated and successfully applied this method in the qualitative and quantification of chlortetracycline in white-tailed deer tissue samples.

This conclusion presents some closing thoughts on the key concepts discussed in the preceding chapters of this book. The book suggests that the rules of engagement with biological and chemical terrorism strategies are stacked against the civilian population when we are considering a war between state-sponsored organizations and terrorist organizations. One of the most challenging obstacles faced by first responders and warfighters is determining the immediate and long-term extent of the region affected by the release of a chemical and/or biological toxin. Combinational protective materials are needed that can be quickly applied and that have the ability to protect the wearer from both biological and chemical attacks. With the advancement of gene technologies, including genetically modified organisms, there are many new and innovative technologies being developed that could provide therapeutics quickly and could effectively target genetically engineered organisms that may be introduced by terrorists.

Pathogenesis describes the mechanisms involved in the production of a disease. This includes the spread of microorganisms and anthropogenic and natural toxins through the body and the physiological responses of the host organism. Many pathogenic agents that cause infectious diseases are considered as ideal selective agents by terrorist groups, which include anthrax, ebola, pneumonic plaque, cholera, tularemia, brucellosis, Q fever, Venezuelan equine encephalitis, smallpox, swine and avian influenza. Biotoxins are naturally occurring toxic agents produced by bioorganisms such as bacteria, cyanobacteria, fungi, and some species of plants and marine fish, which are etiological agents of a variety of animal and human toxicoses. Several biotoxins, such as aflatoxin, botulinum toxin, ricin, and T-2 toxin, have been known to be weaponized. Various levels of training on potential pathologic and toxic agents are needed to ensure that individuals and communities get involved in countermeasure activities and have a good understanding of the potential threats and their role, as well as its consequences.

Electrospun poly (vinyl alcohol) [PVA] nanowebs functionalized with a commercially available microbiocidal solution Reputex™ 20 were prepared. The active ingredient of Reputex™ 20 is polyhexamethylene biguanides, a safe antiseptic. Fourier Transform Infrared spectroscopy confirmed the functionalization of PVA nanowebs. Functionalized nanowebs were characterized by evaluating their antimicrobial properties, breathability characteristics and tensile properties. Functionalized nanowebs demonstrated significant antimicrobial activity against both Gram positive and Gram negative bacteria. Nanowebs developed from biocompatible polymers like PVA, and functionalized with safe antiseptics, could find many biomedical applications such as wound bandages.

We investigated stage-dependent changes in sensitivity of the thyroid gland to perchlorate during development of African clawed frog tadpoles (Xenopus laevis) in relation to non-thyroidal iodide transporting tissues. Perchlorate-induced increases in thyroid follicle cell size and colloid depletion were blunted when exposures began at Nieuwkoop-Faber (NF) stage 55 compared to when exposures began at NF stages 49 or 1-10. To determine if the development of other iodide transporting tissues may contribute to this difference we first examined which tissues expressed transcripts for the sodium dependent iodide symporter (NIS). RT-PCR analysis revealed that NIS was expressed in stomach and small intestine in addition to the thyroid gland of X laevis tadpoles. NIS mRNA was not detected in lung, kidney, skin, gill, muscle, heart or liver. Perchlorate sensitive I-125 uptake was found in stomach, lung, kidney, gill, and small intestine but not muscle, liver, or heart. Perchlorate-sensitive I-125 uptake by stomach was 6-10 times greater than in any other non-thyroidal tissue in tadpoles. While NF stage 49 tadpoles exhibited perchlorate-sensitive uptake in stomach it was roughly 4-fold less than that observed in NF stage 55 tadpoles. Although abundance of NIS gene transcripts was greater in stomachs from NF stage 55 compared to NF stage 49 tadpoles this difference was not statistically significant. We conclude that gastric iodide uptake increases between NF stages 49 and 55, possibly due to post-translational changes in NIS glycosylation or trafficking within gastric mucosal cells. These developmental changes in gastric NIS gene expression may affect iodide availability to the thyroid gland. (C) 2014 Elsevier Inc. All rights reserved.

Standalone poly(vinyl alcohol) (PVA) nanowebs were developed in an environmentally friendly manner, and their breathability values were obtained. The strength of nanowebs was enhanced by heat cross-linking. Breathability values of untreated and heat cross-linked nanowebs remain the same indicating that stronger nanowebs can be developed without compromising its breathability. Transmission FTIR showed an increase in the crystallinity and no significant changes in the structure of PVA nanowebs after heat cross-linking. Stronger and breathable nanowebs can find broader applications such as tissue culture scaffolds, protective clothing liners, and air filters.