Intensifying sodic land characterized by high alkaline pH is an incipient environmental hazard-limiting agricultural potential. In this study, we investigated the effects of plant growth-promoting bacteria Ochrobactrum sp. strain NBRISH6 on the growth and physiology of maize (Zea mays L.) grown under alkaline stress at two soil pH levels. Additionally, we also studied the effects of NBRISH6 on soil fertility parameters. A greenhouse experiment was designed using two live soils (pH 8.2 and 10.2) in earthen pots using maize as a host. Results revealed a significant increase in plant growth and a decrease in defense enzymes in both soil types due to NBRISH6 inoculation as compared to non-treated control. Furthermore, activities of all soil enzymes along with bacterial diversity increased in NBRISH6 treatment under normal as well as stressed conditions. In addition, field evaluation of NBRISH6 inoculation using maize was carried out under normal and alkaline conditions, which resulted in significant enhancement of all vegetative parameters as compared to respective controls. Therefore, the study suggested that Ochrobactrum sp. NBRISH6 can be used to develop a bioinoculant formulation to ameliorate abiotic stresses and enhanced crop productivity.

Urban growth has increased food and chemical demand, which has led to a spike in the production of organic waste. Thus, suitable ways are being investigated for the management of the waste produced. As organic waste is rich in carbohydrates, proteins and minerals, it can be a good source for the growth of microorganisms. Research is being done to produce many value-added products from organic waste by using microbes. It is being used for the production of bioenergy, enzymes, biosurfactants, chemicals, etc., through a microbial fermentation process. The lab demonstrations have shown that organic waste can be a cheaper source for the production of these compounds, and many pre-treatments have been investigated to improve the yield of the products. The biowaste valourisation will solve the issue of organic waste management and could be proven beneficial for the economy.

Agricultural practices are influenced by plant diseases caused by various microorganisms, including nematodes, fungi, bacteria, and viruses, resulting in major crop losses. To maintain a healthy and efficient agricultural system, agrochemicals play a significant role in plant disease management. The intense use of chemicals, however, has detrimental effects on humans and the functioning of the environment and also decreases agricultural sustainability. Sustainable farming is accomplished by reducing or removing fertilizers and agrochemicals, resulting in reduced environmental effects. Special attention has recently been drawn to the use of antagonistic endophytes as biocontrol agents in the form of an attractive alternative for the management of certain plant diseases, resulting in minimal environmental effects. Microbial endophytes correspond to an endosymbiotic group that resides asymptomatically within plant tissues and offers potential biocontrol. Endophytes have been characterized for many bioactive metabolites such as alkaloids, hormones, phenolic acids, and terpenoids, which make them a promising candidate for biotic stress management. Despite these qualities, both efficacy and commercialization still require a deep understanding of the mechanism used by endophytes in protecting the plant from diseases. In the present chapter, we have elucidated different mechanisms employed by endophytes in suppressing microbial diseases in the host plant. This study will also be helpful in developing bioformulations utilizing endophytes as biocontrol agents which, in turn, may contribute to sustainable agriculture.

The establishment of all life forms does not only depend on food supply and security but also on natural resources, and one of them is soil. Mainly in developing countries, due to the increasing demand for more crop production, agriculture is being inclined toward over use of fertilizers and pesticides of chemical origin. Moreover, these fertilizers and pesticides of chemical origin have severely affected soil quality and fertility across the globe. Unpracticed application of these fertilizers, rapid industrialization, untreated waste disposal, and anthropogenic activities are major negative factors in fertility degradation. Thus, to sustain soil fertility with an over dependency on these fertilizers is becoming an alarming issue. Keeping these challenges in mind ecologists, researchers, agriculturists, and other land managers are shifting their attention to restoring soil health and potency through productive and ecofriendly agricultural practices. The plant growth promoting rhizobacteria and their interaction with plants is a well-established phenomenon for plant growth promotion, which occurs either directly or indirectly. Mainly these rhizobacteria colonize around the plant roots without disturbing the native bacterial community. In rhizoremediation (of the type phytoremediation), plant growth promoting rhizobacteria not only reduce the amount of soil contaminating agents but also promote the plant's productivity by releasing certain phytochemicals in stress conditions. This phenomenon primarily occurs through a signaling cascade between plant and microbe via root exudates. Thus, in the growing field of bioremediation, it is an environment friendly, efficient, and cost-effective technique for environmental sustainability.

Intensification of sodic soil due to increasing pH is an emerging environmental issue. The present study aimed to isolate and characterise alkaline stress-tolerant and plant growth-promoting bacterial strains from moderately alkaline soil (pH 8–9), strongly alkaline soil (pH 9–10), and very strongly alkaline soil (> 10). Total 68 bacteria were isolated, and screened for multiple plant growth promoting (PGP) attributes. Out of total, 42 isolates demonstrating at least three plant growth promoting PGP traits selected for further assays. Then out of 42, 15 bacterial isolates were selected based on enhanced maize plant growth under greenhouse experiment, and 16S rRNA gene sequencing revealed Bacillus spp. as a dominant genus. Furthermore, based on improved seed germination percentage and biomass of maize (Zea mays L.) under alkaline stress conditions Alcaligenes sp. NBRI NB2.5, Bacillus sp. NBRI YE1.3, and Bacillus sp. NBRI YN4.4 bacterial strains were selected, and evaluated for growth-promotion and alkaline stress amelioration under greenhouse condition. Amongst the selected 3 plant growth promoting rhizobacterial (PGPR) strains, Bacillus sp. NBRI YN4.4 significantly improved the photosynthetic pigments and soluble sugar content, and decreased proline level in inoculated maize plants as compared to uninoculated control under stress conditions. Moreover, significantly enhanced soil enzymes such as dehydrogenase, alkaline phosphatase and betaglucosidase due to inoculation of Bacillus sp. NBRI YN4.4 in maize plants grown in alkaline soil attributes to its role in improving the soil health. Therefore, alkaline stress-tolerant PGPR NBRI YN4.4 can be useful for developing strategies for the reclamation of saline/sodic soils and improving the plant growth and soil health in sustainable manner.

Plant growth-promoting rhizobacteria (PGPR) improve plant health under various biotic and abiotic stresses. However, the underlying mechanisms of the protective effects of PGPR in deficit water stress (WS) remain less explored. This study aimed to characterize the role of Ochrobactrum sp. NBRISH6 inoculation on maize (Zea mays "Maharaja") under WS conditions using multiple approaches such as physiological, anatomical, metabolic, and molecular. The effect of NBRISH6 inoculation using maize as a host plant was characterized under greenhouse conditions in deficit water stress. Results from this study demonstrated that NBRISH6 significantly lowered the expression of genes involved in the abscisic acid cycle, deficit water stress-response, osmotic stress, and antioxidant enzyme activity (superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase). Phytohormones, i.e. indole acetic acid (IAA) and salicylic acid (SA) levels, intercellular CO2 concentration, metabolites such as simple sugars, amino acids, aliphatic hydrocarbons, and the number of shrunken pith cells modulated in maize roots inoculated with NBRISH6. The NBRISH6 inoculation also improved the plant vegetative properties (root length, 33.80%; shoot length, 20.68%; root dry weight, 39.21%; shoot dry weight, 61.95%), shoot nutrients, xylem cells, root hairs, vapor pressure deficit (75%), intrinsic water-use efficiency (41.67%), photosynthesis rate (83.33%), and total chlorophyll (16.15%) as compared to the respective stress controls. This study provides valuable insights into mechanistic functions of PGPR in WS amelioration and promoting plant physiological response.

The present study aimed to demonstrate the potential of abiotic stress-tolerant Jeotgalicoccus huakuii NBRI 13E for plant growth promotion and salt stress amelioration. NBRI 13E was characterized for abiotic stress tolerance and plant growth-promoting (PGP) attributes under normal and salt stress conditions. Phylogenetic comparison of NBRI 13E was carried out with known species of the same genera based on 16S rRNA gene. Plant growth promotion and rhizosphere colonization studies were determined under greenhouse conditions using maize, tomato, and okra. Field experiment was also performed to assess the ability of NBRI 13E inoculation for improving growth and yield of maize crop in alkaline soil. NBRI 13E demonstrated abiotic stress tolerance and different PGP attributes under in vitro conditions. Phylogenetic and differential physiological analysis revealed considerable differences in NBRI 13E as compared with the reported species for Jeotgalicoccus genus. NBRI 13E colonizes in the rhizosphere of the tested crops, enhances plant growth, and ameliorates salt stress in a greenhouse experiment. Modulation in defense enzymes, chlorophyll, proline, and soluble sugar content in NBRI 13E-inoculated plants leads to mitigate the deleterious effect of salt stress. Furthermore, field evaluation of NBRI 13E inoculation using maize was carried out with recommended 50 and 100% chemical fertilizer controls, which resulted in significant enhancement of all vegetative parameters and total yield as compared to respective controls. Jeotgalicoccus huakuii NBRI 13E is reported for the first time for its ability to develop a bioinoculant formulation for stress amelioration and improved crop productivity.

The study represents a microbial method for reducing heavy metal stress in terrestrial environment. Two rhizobacterial strains Pantoea agglomerance (PC1) and Pseudomonas aeruginosa (SA) having the ability to tolerate Cd2+ and Pb2+ ions stress, were employed in this study. The growth promotion and survival tactics of the strains under metal stress were explored through kinetic growth model using logistic equation, Luedeking-Piret model and Box Behnken design. Study also involves the interaction of strains with Zea mays L under Cd2+ and Pb2+ ions stress. Results revealed that both strains have the potential to tolerate 500 mg L-1 of Cd2+ and Pb2+, ions and maintained the plant growth promoting traits. The Luedeking-Piret model estimated the maximum value of IAA on biomass growth (Y-p/x) 5.377 mu g g(-1) and 10.3 mu g g(-1) under Cd2+ ions, while 7.742 mu g g(-1) and 18.071 mu g g(-1) under Pb(2+ )ions stress for strains SA and PC1, respectively. Further, phosphate solubilization activity was optimized with the help of response surface methodology using Box Behnken Design. The optimum solubilization by strain PC1 and SA was achieved at 100 and 150 mg L-1 of Cd2+, and 150 and 200 mg L-1 of Pb2+ ion concentration at the pH range 6.75 and 7.5 respectively. The interactive study with Zea mays L. showed significant increase in seed germination in the presence of Cd2+ and Pb2+ ions thereby proving them as potent plant growth promoters and metal stress reducing biological agents. Hence, the findings of the study suggest that rhizobacterial strains could be a sustainable tool for restoration of metal contaminated sites.

A comprehensive survey for 09 agro-climatic zones of Uttar Pradesh, India was conducted to isolate and characterize salt tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase possessing plant growth promoting (PGP) rhizobacteria for salt stress amelioration in rice. Here, we have isolated 1125 bacteria having the ability to tolerate 1M NaCl and out of those, 560 were screened for utilizing ACC as sole nitrogen source. 560 isolates were subjected for bacteria coated seed germination assay under 100mM salt (NaCl) stress resulting to 77 isolates which were further evaluated for seed germination assay, PGP and abiotic stress tolerance ability in vitro. This evaluation revealed 15 potent rhizobacteria representing each agro-climatic zone and salt stress mitigation in vitro. In particular, the biomass obtained for bacteria coated rice seedlings were corroborated with the performance of isolates exhibiting maximum average indole acetic acid (IAA) production respective to the agro-climatic zone. Surprisingly based on 16S rRNA, much of the propitious isolates belonged to same specific epithet exhibited variedly in their characteristics. Overall, Bacillus spp. was explored as dominant genera in toto with highest distribution in Western Plain zone followed by Central zone. Therefore, this study provides a counter-intuitive perspective of selection of native microflora for their multifarious PGP and abiotic stress tolerance abilities based on the agro-climatic zones to empower the establishment and development of more suitable inoculants for their application in agriculture under local stress environments.

Emerging environmental issues related to heavy metal contamination in rice draw great concern about the soil quality of paddy farming lands irrigated with groundwater. Investigating the functioning of soil microorganisms exposed to heavy metal contamination is imperative for agricultural soil manipulations. The current study accentuates the influence of heavy metals on microbial activity and community composition in arable soil of West Bengal State of India. The result revealed that the fertility indicators (activity of all soil enzymes) and growth-limiting factors (soil N and P) were negatively correlated with the heavy metal stress except the soil total organic content which demonstrated significant positive correlation with the heavy metals. In case of functional diversity of soil, all the considered diversity indices exhibited no specific pattern along with the availability of heavy metals. Further, despite the heavy metal contamination, we observed a very complex and indifferent pattern of bacterial community composition along the heavy metal contamination sites. Overall, we found that γ-Proteobacteria had been the most abundant bacterial community followed by Actinobacteria, Firmicutes, β-Proteobacteria and α-Proteobacteria. Commemorating all the results, we can infer that arsenic and other heavy metal contamination is deteriorating the soil quality and hence warrants immediate attention of concerned soil scientist and agronomists.

Two plant growth promoting rhizobacteria (PGPR) Pseudomonas putida NBRIRA and Bacillus amyloliquefaciens NBRISN13 with ability to tolerate abiotic stress along with multiple PGP traits like ACC deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. Earlier we have reported both the strains individually for their PGP attributes and stress amelioration in host plants. The present study explains in detail the possibilities and benefits of utilizing these 2 PGPR in consortium for improving the chickpea growth under control and drought stressed condition. In vitro results clearly demonstrate that both the PGPR strains are compatible to each other and their synergistic growth enhances the PGP attributes. Greenhouse experiments were conducted to evaluate the effect of inoculation of both strains individually and consortia in drought tolerant and sensitive cultivars (BG362 and P1003). The growth parameters were observed significantly higher in consortium as compared to individual PGPR. Colonization of both PGPR in chickpea rhizosphere has been visualized by using gfp labeling. Apart from growth parameters, defense enzymes, soil enzymes and microbial diversity were significantly modulated in individually PGPR and in consortia inoculated plants. Negative effects of drought stress has been ameliorated and apparently seen by higher biomass and reversal of stress indicators in chickpea cultivars treated with PGPR individually or in consortia. Findings from the present study demonstrate that synergistic application has better potential to improve plant growth promotion under drought stress conditions.

Soil bacteria may have properties of plant growth promotion but not be sufficiently beneficial for plants under stress conditions. This challenge has led researchers to extend their searches into extreme environments for potential soil bacteria with multiple plant beneficial traits as well as abiotic stress tolerance abilities. In the current study, an attempt was made to evaluate soil bacteria from an extreme environment, volcano soils, based on plant growth promoting and abiotic stress mitigating characteristics. The screening led to the isolation of eight (NBRISH4, NBRISH6, NBRISH10, NBRISH11, NBRISH13, NBRISH14, NBRISH16 and NBRISH26) bacterial isolates capable of withstanding stresses, namely temperature (up to 45 °C), salt (up to 2 M NaCl) and drought (up to 60% Poly Ethylene Glycol 6000) in vitro. Further, the selected isolates were notable for their in vitro temporal performance with regards to survival (in terms of colony count), phosphate solubilisation, biofilm formation, auxin, alginate and exo-polysaccharide production abilities under abiotic stresses i.e. 40 °C temperature; 500 mM NaCl salt and drought (PEG) conditions. In vivo seed treatments of individual selected bacteria to maize plants resulted into significant enhancement in root and shoot length, root and shoot fresh and dry weight and number of leaves per plant. Overall, the plant growth promoting and abiotic stress tolerance ability was most evident for bacterial isolate NBRISH6 which was identified as an Ochrobactrum sp. using 16S rRNA based phylogenetic analysis.