Nicotiana tabacum leaves are mainly used in the production of cigarettes and cigars all over the world, and a large amount of waste materials will be produced in the process. High-value scopoletin can be extracted and separated from waste materials, but there are some problems, such as low purity of products and inability of large-scale production. In this study, we established an efficient and stable method for producing high-purity scopoletin from waste tobacco by containing acetone reflux extracting with dynamic axial compression-high performance liquid chromatography (DAC-HPLC) separating. In particular, the extraction system makes the scopoletin fully extracted through continuous reflux, the DAC-HPLC system makes the high-purity scopoletin repeatedly obtained, and the DAC-HPLC system was first used for scopoletin. Finally, related to the waste tobacco power, the yield of scopoletin is 1.9

目的:考察家蚕蛹虫草(海宁株)子实体提取分离获得的麦角甾醇和虫草素联合抗肿瘤作用.方法:以家蚕蛹虫草(海宁株)子实体提取分离获得的麦角甾醇和虫草素为原料,开展联合给药(麦角甾醇:虫草素=3:2)的小鼠急性毒性试验,进一步构建lewis肺癌和H22肝癌荷瘤小鼠模型,以环磷酰胺作为阳性对照,分别建立模型组、试验组、空白组,考察联合给药对小鼠肺癌和肝癌的药效.结果:根据Bliss方法计算急性毒性试验数据,获得联合给药的LD50为3.98 g/kg.此外,药效试验结果表明,联合给药剂量在67～600 mg/kg内时,抑瘤率随给药剂量的升高而增强;当给药剂量为600 mg/kg时,与模型组相比,对小鼠肺癌和肝癌的抑制率最高,分别为50％和46％,其对小鼠Lewis肺癌模型的疗效优于环磷酰胺,然而,对小鼠H22肝癌肿瘤模型的效果与环磷酰胺无明显差异.结论:初步试验结果表明,在安全剂量范围内,家蚕蛹虫草(海宁株)子实体中麦角甾醇与虫草素联合给药具有抗肺癌肿瘤及肝癌肿瘤生长的作用,为肺癌和肝癌药物的开发及应用建立良好的基础.

以中药材白芨粉为原料,采用水提醇沉原理生产白芨胶.根据白芨水提取—水浓缩—乙醇提取—乙醇浓缩—白芨胶获得等工艺步骤,设计可满足不同需求的、规模化、智能化白芨胶生产系统,生产系统包括提取罐、真空上料机、离心机、浓缩器、醇沉分离一体罐、离心液储罐和浓缩液储罐.以572 kg贵州白芨为原料,通过该生产系统获得白芨胶冻干粉207.11 kg,该白芨胶冻干粉总糖含量为84.13％.建立了白芨胶的生产工艺,通过该工艺生产出得率更高、总糖含量高的白芨胶冻干粉,同时根据生产实际设计了白芨胶提取分离一体化生产线,这为白芨胶的规模化生产建立了坚实的基础.