With the growing global demand for advanced wound management solutions, bioactive sutures have been developed to promote tissue regeneration. However, simultaneously integrating wound monitoring and therapeutic capabilities into a single suture remains challenging. Herein, we developed a biomimetic suture inspired by spider silk, featuring a heterogeneous core-shell structure composed of polyurethane (PU), carbon nanotubes (CNTs), polydopamine (PDA), two-dimensional transition metal carbides/nitrides (MXene), and indocyanine green (ICG), which integrates ultrasensitive strain sensing with near-infrared (NIR) light-triggered antibacterial functionality. The conductive networks were established jointly by CNTs and MXene layers, exhibiting sufficient strength (ultimate breaking strain up to 993.8%), excellent sensing sensitivity (maximum gauge factor value of 15.36 at 0–50% strain), high cycling stability (≥ 1000 cycles), and satisfying duration even in wet conditions (up to 7 days in simulated body fluids). Ex vivo experiments on porcine skin further confirmed the effectiveness of the sutures in monitoring wound strain. Furthermore, the photodynamic therapy (PDT) and photothermal (PTT) capabilities of the ICG/MXene/PDA/CNTs system enabled on-demand antibacterial treatments, achieving over 95% antibacterial efficiency against S. aureus and E. coli in both in vitro and in vivo studies. This work highlights the potential of the smart sutures for wound monitoring and management, while providing valuable insights into the development of integrated diagnostic and therapeutic sutures.

AIM:To investigate the underlying mechanism of immune cell crosstalk in periodontal inflammatory ageing and to explore potential pharmaceutical interventions for safely reversing this process. MATERIALS AND METHODS:Bovine serum albumin-epigallocatechin gallate-copper nanoparticles (BEC NPs) were synthesised by coating a bovine serum albumin membrane onto an epigallocatechin gallate-copper-phenolic network. BEC NPs' regulatory impacts on the fate of neutrophils and macrophages were evaluated through immunofluorescence staining, PCR, RNA sequencing and Western blot analysis. Their anti-senescence effects on gingival fibroblasts were assessed using cell migration assays and SA-β-gal staining, while an experimental periodontitis rat model was established to validate the in vitro findings. RESULTS:Accumulating evidence indicated that pH-responsive nanoparticles alleviated periodontal inflamm-ageing through modulation of neutrophil-macrophage crosstalk. Functional analyses revealed that BEC NPs suppressed neutrophil extracellular trap formation via dual mechanisms: reactive oxygen species scavenging to sustain mitochondrial integrity, and actin cytoskeleton stabilisation to inhibit nuclear translocation of neutrophil elastase and myeloperoxidase. In vivo assessment demonstrated that BEC NPs exhibited a favourable biosafety profile and significant therapeutic efficacy in suppressing the progression of periodontal inflammation. CONCLUSION:This study presents a smart nanosystem-based 'endogenous homeostasis reconstruction' strategy, offering programmable, early-stage intervention for periodontal inflamm-ageing with considerable clinical translation prospects.

The regeneration of critical-size irregular bone defects, especially those with irregular shapes, remains clinically challenging due to inadequate shape-conformability, immune dysregulation, poor vascularization and the unsatisfied osteogenic bioactivity. Self-fitting tissue scaffolds that can be implanted by minimally invasive procedures are a promising solution. Herein, we present a 4D-printed hydrogel scaffold (PASN) composed of polydopamine (PDA), alginate (Alg), and strontium ions (Sr2⁺), that is 3D-printed and NIR-induced shape-morphing to match defect and orchestrate both immunomodulation and osteogenesis. Compared with traditional hydrogels, this PASN scaffold enables shape morphing with quantitatively tunable curvature and controllable deformation speed responsible to NIR light, while functionally fitting irregular bone defects. By eliminating reactive oxygen species and driving M2 macrophage polarization through PDA, PASN improves immune regulation through the PI3K signaling pathway as well as stimulates osteogenesis and angiogenesis through release of Sr2⁺. The deformation angle of the scaffold is adjusted by the laser power, irradiation time, and the designed pattern of alginate PDA struts, whereas the temperature gradient formed between the upper and lower layers under NIR irradiation induces bending toward the light source. Dome-shaped calvarial defects, maxillary sinus floor lift and horizontal bone augmentation surgeries demonstrate the PASN scaffold markedly enhances bone regeneration. Collectively, the personalised and bioactive scaffold with NIR-responsive shape-morphing may be a promising biomaterial for clinical-size and irregular bone defect regeneration.

The repair of bone defects presents a complex challenge, requiring implants that satisfy anatomical, functional, and long-term stability demands. To address this need, polyaryletherketone (PAEK) has emerged as a promising...

Inflammation, especially invasive bacterial inflammation, has always been a hot topic in medical research. With the emergence of superbugs and highly virulent viruses, current treatment methods are gradually revealing areas of deficiency. As an inflammatory form of cell death, the concept of pyroptosis offers new possibilities for the treatment of inflammation. Pyroptosis is typically triggered by inflammasomes and executed by the Gasdermin (GSDM) family. It is characterized by membrane perforation, cell swelling, and the release of cellular contents such as pro-inflammatory factors. Pyroptosis plays a crucial role in host immune defense, inflammatory diseases, and tumor regulation. In this review, we comprehensively explored each member of the GSDM family and its activation pathways, reviewed the cellular consequences of GSDM activation, and the role of GSDM-mediated pyroptosis in inflammatory diseases such as sepsis. We also provided a detailed account of the latest advancements in inflammation control targeting GSDM, aiming to offer new perspectives for precise therapies targeting pyroptosis.

AIM:To compare the percentage of new bone formation as the primary outcome of bone regeneration at 6 months after maxillary sinus floor elevation (MSFE) using platelet-rich fibrin (PRF) combined with deproteinised bovine bone mineral (DBBM) versus DBBM alone, and to explore the molecular mechanisms through which PRF modulates osteogenesis in the maxillary sinus. MATERIALS AND METHODS:Forty patients undergoing MSFE were randomly allocated to two groups: DBBM alone or PRF + DBBM. Six months post MSFE, bone core samples were harvested during implant placement. Clinical outcomes included implant stability and postoperative complications. Radiographic analyses quantified graft height and volumetric changes. Bone regeneration was evaluated by micro-computed tomography (micro-CT) and histomorphometry, while osteogenic marker expression was examined using RT-qPCR and immunofluorescence. Proteomic profiling, followed by Western blot validation, was performed to identify signalling pathways associated with PRF-induced osteogenesis. RESULTS:The PRF + DBBM group (n = 20; 42 implants) showed significantly higher primary implant stability (71.85 ± 4.96 vs. 67.65 ± 5.19, p < 0.05). Histological analysis revealed a significantly greater amount of newly formed bone with improved quality, as indicated by higher new bone formation (28.81% ± 4.41% vs. 22.44% ± 4.40%, p < 0.001) and increased bone maturity (57.05% ± 7.76% vs. 49.17% ± 7.09%, p < 0.01) compared to the DBBM group (n = 20; 45 implants). Molecular assays showed up-regulated osteogenic gene and protein expression, with activation of TGF-β, PI3K-Akt and complement and coagulation cascade pathways. CONCLUSION:PRF combined with DBBM resulted in improved new bone formation in the maxillary sinus compared to DBBM alone and showed superior healing outcomes, potentially mediated by key growth factors and the complement protein C1q.

Chronic wound healing remains a significant clinical challenge due to persistent inflammation, oxidative stress, mitochondrial dysfunction, and cellular senescence. A near-infrared (NIR)-responsive polydopamine-resveratrol (PDA@RES) core-shell nanoplatform was developed to address these interconnected pathological mechanisms through synergistic photothermal therapy and drug delivery. The nanoplatform exhibited excellent photothermal conversion capability and demonstrated superior antioxidant and anti-inflammatory effects, effectively scavenging intracellular reactive oxygen species (ROS), restoring mitochondrial membrane potential, and repolarizing macrophages toward a pro-healing phenotype. Mechanistically, the platform activated the AMPK/PGC-1 alpha signaling axis, initiating programmed mitochondrial homeostasis remodeling through enhanced mitophagy and biogenesis, thereby blocking senescence-inducing signals and reversing cellular senescence. The immune microenvironment remodeling subsequently promoted vascular endothelial cell migration and angiogenesis. In diabetic rat models, the NIR-responsive nanoplatform significantly accelerated wound healing by promoting collagen deposition, balancing the immune microenvironment, and facilitating functional vascular regeneration. Notably, the treatment induced nascent hair follicle structures, achieving high-quality regenerative healing rather than scar formation. This study provides an efficient, multi-target synergistic therapeutic strategy for chronic wound healing.

Therapeutic angiogenesis in inflammatory microenvironments is constrained by mitochondrial dysfunction in mesenchymal stem cells (MSCs). This study demonstrates that platelet-rich fibrin (PRF) serves as a mitochondrial reservoir that transfers functional mitochondria to dental pulp stem cells (DPSCs) via extracellular vesicle-dependent mechanisms. Multi-omics analyses revealed that PRF-derived mitochondria activated the tricarboxylic acid (TCA) cycle in DPSCs, driving concurrent fatty acid biosynthesis and JAK2/STAT4-mTOR pathway activation. This metabolic-signaling integration enhanced VEGF secretion and cell migration under inflammatory conditions. PRF's fibrin matrix further sustained mitochondrial release while providing topological guidance for DPSC recruitment. In vivo, PRF-DPSC composites significantly accelerated wound closure and neovascularization compared to controls, supported by histomorphometric and molecular analyses. Beyond cytokine delivery, this work establishes PRF as a mitochondrial-augmented biomaterial to reverse MSC metabolic insufficiency, offering a translatable strategy for vascular regeneration in hostile microenvironments.

Reconstruction of keratinized mucosa (KM) with sufficient dimensions is critical for long-term periodontal and peri-implant health. However, existing biomaterials struggle to recapitulate the complex biophysical and biochemical microenvironment of KM while achieving stable adhesion and integration in the wet and mechanically dynamic oral cavity. Here, we design a photocrosslinkable double-network hydrogel composed of methacrylated platelet-rich fibrin (iPRF-MA), N-hydroxysuccinimide-functionalized alginate (Alg-NHS), and luteolin-loaded epigallocatechin gallate microspheres (Lut@EGCG) to enable KM regeneration in the challenging oral environment through dual microenvironmental modulation. At the material level, the covalent network from iPRF-MA and the supramolecular network based on Alg-NHS work synergistically, resulting in strong wet tissue adhesion and high fatigue resistance, which collectively prevent hydrogel dislodgement under oral dynamic stresses. Biochemically, the hydrogel enables sustained release of growth factors and EGCG, synergistically enhancing angiogenesis and immune regulation, while also redirecting neutrophil phenotype toward a phagocytic state for specific antibacterial activity. Biophysically, the hydrogel provides gingival fibroblasts with a mechanically instructive microenvironment that activates mechanotransduction signaling and accelerates extracellular matrix remodeling. In vivo experiments confirm outstanding KM regeneration following treatment with the double-network hydrogel. This study demonstrates a microenvironment-targeting strategy for KM reconstruction through rational hydrogel design, offering a therapeutic platform for functional KM regeneration.

With accelerating population aging, age-related bone loss, osteoporosis, and delayed bone defect repair have become major challenges in regenerative medicine. Bone aging is not caused by the decline of a single cell type, but is a multilevel pathological process driven by the continuous coupling and mutual reinforcement of intracellular damage accumulation, senescence signal propagation, and microenvironmental deterioration. Based on established aging theories, this review integrates regenerative impairment in aged bone into three interconnected pathological cycles from the perspectives of dynamic feedback and regenerative intervention. Intracellular damage and homeostatic imbalance promote cellular senescence; senescent cells spread senescence signals through SASP and immune dysregulation, inducing abnormalities in metabolism, extracellular matrix (ECM) structure, and intercellular communication; the deteriorated microenvironment then further aggravates intracellular damage and homeostatic disruption, forming a self-reinforcing pathological loop. Within this framework, we summarize biomaterial strategies targeting intracellular damage, senescent cells and SASP-mediated propagation, and the aged microenvironment, and discuss multifunctional and responsive materials for staged or multi-level intervention. This framework organizes dispersed aging mechanisms into a dynamic network of feedback relationships and actionable nodes, providing guidance for mechanism-oriented biomaterial design and strategy selection in aged bone regeneration.

Periodontitis is a chronic inflammatory oral disease characterized by irreversible alveolar bone resorption, which severely impairs oral health and even leads to tooth loss. Clinical bone augmentation therapy for alveolar bone defects mainly relies on exogenous bone substitute materials. However, traditional materials are prone to implantation failure due to the persistent inflammatory microenvironment and bacterial infection in the periodontal area. The addition of antibiotics to improve antibacterial properties not only induces bacterial resistance but also causes systemic toxic and side effects, making it difficult to meet clinical treatment needs. This review focuses on the core demand for bone substitute materials in periodontitis treatment to simultaneously achieve antibacterial, anti-inflammatory, and osteogenic functions—an essential characteristic that distinguishes such materials from conventional antibacterial drugs and single-function bone graft materials. We systematically elaborate on multiple interrelated inflammatory signaling pathways (e.g., RANKL/RANK/OPG, cGAS-STING, NF-κB, JAK-STAT, MAPK, PI3K/AKT, HIF-1, TGF-β/SMAD, Wnt/β-catenin, Hippo) and inflammasome mechanisms involved in periodontitis-associated alveolar bone resorption, exploring potential targets for screening excellent anti-inflammatory and osteogenic active molecules. On this basis, we summarize modification strategies for bioceramic bone tissue-engineered substitutes, including incorporating metal ions (Ag, Cu, Sr, Mn, Mg, Zn, etc.) and natural anti-inflammatory molecules into the material matrix to enhance their multifunctional properties while maintaining favorable physical and biological characteristics. We also discuss structural and functional optimization of composites via surface morphology modification, photothermal, and photodynamic coating construction to improve adaptability to the periodontal inflammatory microenvironment. These reconstructed multifunctional composite bioceramic materials integrate antibacterial, anti-inflammatory, and osteogenic functions, eliminating periodontal bacterial infection, alleviating local chronic inflammation, and actively promoting osteoblast differentiation and alveolar bone defect repair, thereby perfectly matching the pathological characteristics of periodontitis. This review clarifies the core design concept of multifunctional integration for bioceramic bone tissue-engineered substitutes in periodontitis treatment and provides a theoretical basis and technical reference for developing novel bone graft materials with clinical transformation potential.

[This corrects the article DOI: 10.3389/fbioe.2024.1535207.].

Jawbone defects pose significant challenges for oral implantation and restoration. Guided bone regeneration is the most common strategy for bone augmentation. However, inadequate efficiency of osteogenesis remains a persistent issue. Periosteum serves as a potential source of osteogenesis, but insufficient mesenchymal stem cells (MSCs) in periosteum often exist due to the lack of initial bone support. Inadequate blood supply near the submembranous osteogenic region also impedes the osteogenesis process. This paper proposes a dual cross-linked natural protein-derived hydrogel, GelMA-fibrinogen/NELL-1(GFN), which optimizes both physical and biological factors to stabilize the elevated maxillary sinus membrane (MSM) (an anatomical structure involved in osteogenesis in maxillary posterior dental region, resembling periosteum) and support the osteogenic space. The results showed stem cells derived from the membrane could migrate to GFN and exhibit robust proliferation capability. Under osteogenic induction, the adequately expanded MSM-derived MSCs enhanced osteogenic differentiation under the synergetic guidance of fibrinogen and Nell-1 through TGF-β/HIF-1α/β-catenin signaling pathway. In vivo experiments showed that GFN could promote bone formation with double-layered cortical bone structures in maxillary sinus region, which provides a new direction for construction of bone grafting materials.

Bacterial biofilm colonization and persistent chronic inflammation in periodontitis cause periodontal tissue destruction and eventual tooth loss. Bacterial biofilms disruption and immune cell metabolism reprogramming are critical for the treatment of periodontitis. Herein, we developed an injectable MXene-based hydrogel (GQM), composed of oxidized gellan gum, quaternized chitosan, and magnesium-tannic acid-modified MXene nanosheets (MTA-Mg), which serves as a flexible scaffold for targeted delivery of MTA-Mg within periodontal pockets. The GQM hydrogels can be injected into periodontal pockets and targetedly deliver MTA-Mg, which disrupts the dense biofilm efficiently by photothermal effect, while the bacteria are killed through the electrostatic interaction and charge neutralization from quaternized chitosan. MTA-Mg nanosheets serve as interfacial electron transfer to activate oxidative phosphorylation pathway, while delivering the magnesium and tannic acid to improve mitochondrial function, which reprogram the immune cell metabolism by inducing macrophage toward the M2 phenotype. The rat periodontitis model demonstrated that the GQM hydrogel effectively eradicates bacterial biofilms, alleviates inflammation, and reverses alveolar bone resorption, thereby treating periodontitis efficiently. All in all, the GQM hydrogel achieves a synergistic effect of "biofilm disruption-immune metabolic reprogramming" and offers a novel strategy for the reversal of inflammatory bone resorption in periodontitis.

Ectodermal dysplasia (ED) often results in congenital tooth agenesis and severe developmental alveolar ridge defects, leading to functional impairments, reduced masticatory efficiency, and psychosocial challenges. Limited local blood supply and rapid bone resorption in ED patients pose significant challenges for predictable implant rehabilitation, highlighting the need for biologically guided, long-term restorative strategies. An 18-year-old male patient with hypohidrotic ED (HED) presented with multiple congenitally missing teeth (FDI 12–16, 22–26, 34–35, 42, 44–46) and knife-edge alveolar ridges. Following two years of fixed orthodontic treatment and maxillary sinus augmentation at an external clinic, the patient underwent simultaneous alveolar ridge augmentation with implant placement at our institution. Subsequently, implant-supported fixed prostheses were delivered. Multidisciplinary sequential treatment achieved favorable clinical outcomes (Table 1). At one-year follow-up after final prosthesis placement, all implants demonstrated stable osseointegration, marginal bone loss < 0.5 mm, probing depths < 3 mm, and bleeding on probing < 15

Critical-sized bone defects pose a significant clinical challenge, with inadequate blood supply being a major factor impairing bone healing. In the initial stage of bone healing, the blood clot acts as a "natural glue," serving not only as a scaffold for stem cell growth but also providing a reservoir of cytokines and growth factors that regulate and reconstruct the osteogenic microenvironment. In this study, we hierarchically integrated platelet concentrates with distinct functions into an injectable thermosensitive hydrogel, constructing a "biomimetic blood clot" system. First, leukocyte-platelet-rich fibrin (L-PRF) lyophilized powder, which promotes the osteogenic differentiation of periosteum-derived mesenchymal stem cells (PMSCs), was incorporated into methacrylated gelatin hydrogel microspheres (GM) to form GML-PRF. Subsequently, advanced platelet-rich fibrin (A-PRF) lyophilized powder, which enhances the angiogenic differentiation of lamina propria-derived mesenchymal stem cells (LMSCs), was blended into a hydrogel precursor composed of chitosan (CS), polygalacturonic acid (PgA), β-glycerophosphate (β-GP), and GML-PRF. This yielded a thermosensitive hydrogel, designated CSPgAA-PRFGML-PRF (CPAGL), which exhibits favorable mechanical properties at 37 °C and enables the programmed release of growth factors from both A-PRF and L-PRF. The CPAGL hydrogel could induce the differential differentiation of distinct types of MSCs. It facilitates vascularized bone regeneration by activating the JAK2-STAT3/HIF-1α and the TGF-β/Wnt signaling pathway, thereby effectively initiating the coupled angio-osteogenic process. In summary, the biomimetic blood clot system CSPgAA-PRFGML-PRF represents a potential injectable biomaterial strategy for bone defect repair.

Non-porous biomaterials, including conductive hydrogels, feature a highly porous three-dimensional (3D) structure, good biocompatibility, and conductivity. Electrical conductivity is achieved by electron or ion transport within the hydrogel network, and through this capability, conductive hydrogels can mimic the bioelectrical environment of the human body and can regulate cell functions. These bioelectrical stimuli are crucial for wound healing and regeneration, especially in the cardiovascular system, nervous system, bone and skin. Thus, the properties and conductivity of conductive hydrogels must be comprehensively understood to aid in the development of new biomaterials. We here review primarily two types of conductive hydrogels: electronically conductive hydrogels and ionically conductive hydrogels. It also outlines recent progress in their use for repair of cardiac tissue, nerves, bone and cartilage, and of skin wounds. This review describes the potential of conductive hydrogels to promote adhesion, proliferation, differentiation, and electrical stimulation of cells. In particular, these hydrogels can provide electrical conductivity to repair damaged cardiac tissue, enhance axonal regeneration, promote bone and cartilage repair, and promote chronic wound healing. These hydrogels also have unique properties such as antibacterial properties, water retention, and intelligent sensing. The review ends with a discussion of the main challenges facing this field, such as poor mechanical stability, expensive production cost, unsatisfactory long-term degradation and the lack of intelligent regulation, and it suggests possible strategies to overcome these challenges. Moving forward, we need to focus more on simplifying the fabrication procedure, enhancing the stability of the material, and enabling personalized and intelligent property through molecular design. This can be achieved through collaboration with other disciplines to promote clinical translation and to offer safer and efficient strategies for tissue repair.

The refractory nature of periodontitis stems from two interrelated factors: the difficulty in eradicating deeply entrenched pathogenic biofilms and the biofilm-induced impairment of mitochondrial autophagy in immune cells, leading to metabolic dysregulation and persistent inflammation. These processes mutually reinforce each other, creating a self-perpetuating vicious cycle. To address this, we developed a spatiotemporally programmable smart hydrogel (GM hydrogel), constructed on a dynamically crosslinked network of oxidized fucoidan and carboxymethyl chitosan, loaded with silver nanoparticles and EGCG-modified MXene nanosheets (MXene@EGCG-Ag). This near-infrared (NIR) light-responsive platform exhibits excellent injectability, enabling it to completely fill narrow, deep, and irregular periodontal pockets, ensuring intimate contact with pathological sites. The GM hydrogel provides programmed control along both temporal and spatial dimensions. In the temporal dimension, NIR irradiation triggers MXene-mediated mild photothermal effects (<45 °C) that disrupt biofilm structure and facilitate Ag+ and EGCG penetration into deeper tissues. In subsequent stages, sustained EGCG release restores mitochondrial autophagy, reprogramming immune cell metabolism to improve the immune microenvironment. Spatially, the hydrogel penetrates mature biofilms and delivers comprehensive treatment from the surface through deep gingiva to the alveolar bone interface. Experimental results demonstrate that GM hydrogel disrupts ionic homeostasis and impairs biofilm functionality in Porphyromonas gingivalis, exhibiting potent antibacterial effects. Sustained EGCG release activates the PINK1/Parkin-mediated FOXO pathway, which restores mitochondrial autophagy and induces metabolic reprogramming, thereby suppressing inflammation and promoting alveolar bone regeneration.

Enhancing the spatiotemporal control of drug delivery holds significant potential for improving drug bioavailability, minimizing adverse reactions and toxicity, and advancing precision medicine. Traditional hydrogels have been widely recognized for their significance in substance delivery, attributed to their outstanding biocompatibility. Nevertheless, they exhibit certain limitations in the areas of controlled release and cell delivery. Cryogels, prepared using freezing technology, exhibit a unique macropores structure. The variations in technical parameters, such as the concentration of the gel precursor solution, freezing temperature, and freezing time facilitate adjustable mesh sizes and high pore connectivity. These characteristics are posited to enhance cell migration into the hydrogel interior and promote the transport of nutrients and metabolic waste. Their excellent toughness and injectability facilitate stress dispersion and provide effective cell protection. Consequently, cryogels modify drug release dynamics via their unique structure and integrate multiple release control mechanisms, thereby achieving spatiotemporal controlled delivery. This review paper provides an overview on synthesis methods, features, delivery advantages and mechanisms of cryogels. It further emphasizes the latest research advancements and applications of cryogels in spatiotemporal controlled delivery while acknowledging limitations associated with existing application methods.

The posterior mandible is the primary area for occlusal function. However, long-term tooth loss in the posterior mandible often leads to rapid absorption of both buccal and lingual trabecular bone plates and subsequent atrophy of the alveolar ridge. This ultimately results in horizontal bone deficiencies that complicate achieving an optimal three-dimensional placement for dental implants. Conventional techniques employed clinically for horizontal bone augmentation have limited efficacy, cause significant surgical trauma, and require extended treatment duration. Consequently, the selection of an effective and minimally invasive bone augmentation technique for restoring bone width is an essential prerequisite for successful implant restoration in the posterior mandible. This clinical case study presented a treatment approach involving guided bone regeneration (GBR) and in situ Onlay grafting for bone level augmentation in the blade-shaped alveolar ridge of the posterior mandible, followed by implant restoration. By rotating the in situ sourced bone block, the denser bone volume at the base of the blade-shaped alveolar ridge was transferred to the crest of the alveolar ridge, obviating the necessity for a secondary operative site and mitigating complications such as pain, edema, sensory abnormalities, and nerve injury. Incorporation of trabecular bone within the recipient area enhanced fixation while augmenting vascular supply. A significant increase in bone volume by 1,628.21 mm3 was achieved within 7 months postoperatively. Overall, this novel approach offers valuable insights into minimally invasive and stable techniques for alveolar bone augmentation.