为研究miR-9-5p靶向沉默信息调节因子2相关酶1(silent information regulator factor 2-related enzyme 1,SIRT1)对骨关节炎(osteoarthritis,OA)软骨细胞凋亡的影响及相关机制,对小鼠的原代软骨细胞进行分离、培养并构建OA细胞模型(加入IL-1β)和OA小鼠模型,检测miR-9-5p的表达、Caspase-3活性、细胞活性及凋亡.确定miR-9-5p和SIRT1存在靶向关系后检测SIRT1活性和上述指标.用番红O-固绿对OA小鼠组织进行染色并分析,在OA小鼠模型中再次验证miR-9-5p通过抑制SIRT1对软骨细胞凋亡的影响.结果显示,miR-9-5p mRNA在OA组织及IL-1β处理的软骨细胞中表达显著升高(P＜0.001),miR-9-5p可抑制OA软骨细胞活性(P＜0.001)并促进其凋亡(P＜0.01).miR-9-5p靶向作用于SIRT1,并通过抑制SIRT1抑制OA软骨细胞活性(P＜0.001),促进其凋亡(P＜0.05).在OA动物模型的验证中得到相同的结果.该研究提示,miR-9-5p可通过靶向SIRT1抑制OA软骨细胞活性,促进OA软骨细胞凋亡,参与OA的发生和发展.

输血传播病毒(transfusion transmitted virus,TTV)是一种新的DNA病毒,通常由输血途径传播,其基因组变异率与RNA病毒相似,但是很难预测其致病力是否会朝着增强的方向发展.本研究探讨了TTV病毒与类风湿关节炎患者之间的关系.研究发现类风湿性关节炎患者存在TTV感染,其感染率显著高于正常人群,性别上无明显差异,不存在某种特定基因型感染,初步明确了类风湿性关节炎患者和正常人群对TTV的感染率,并进一步探讨了该病毒基因分型对类风湿性关节炎患者和正常人群两组人群的影响.

Objective To explore the characteristics of transfusion transmitted virus molecular epidemiology in the fourth and five groups in Kunming District of Yunnan through the examination of blood transfusion transmission.Methods the detection of serum transfusion transmitted virus by polymerase chain was analyzed,and the homology and phylogenetic analysis were analyzed after the positive samples were sequenced.Results the study of 223 sera to be detected,and according to the sequence determination and comparison,the clear 150 positive samples,the HIV infection rate of 64.38％,the fourth group detection out of 47,the transfusion transmitted virus infection rate of 21.08％;the fifth group detection out of 77,the transfusion transmitted virus infection rate of 34.53％;mixed infection of nine infection rate of 4.04％.Conclusion the data about the epidemiology of transfusion transmitted virus in fourth and five groups were obtained,which could prevent the transmission of transfusion transmitted virus.

Objective To investigate the imbalance of Th1/Th2 cell response in patients with systemic lupus erythematosus (SLE) combined with coronary heart disease .Methods SLE patients ,SLE patients with coronary heart disease and healthy con-trols were enrolled and blood samples were collected .T-bet/GATA-3 ,the transcription factors of Th1/Th2 cells ,were detected by real-time PCR ;the intracellular cytokines IFN-γ and IL-4 in CD4 + T cells were stained by fluorescent antibodies and detected by flow cytometry ;the level of serum IFN-γ and IL-4 were detected by ELISA .Results Comparing with healthy control group ,the ex-pression level of Th1 transcription factor T-bet ,the introcellular secretion of IFN-γ in CD4 + T cells and the serum IFN-γ were all decreased in non-coronary heart disease patients with SLE( P < 0 .05) .Comparing with non-coronary heart disease patients with SLE or healthy control group ,the expression level of Th1 transcription factor T-bet ,the introcellular secretion of IFN-γ in CD4 + T cells and the serum IFN-γ were all increased in patients with SLE combined coronary heart disease(P< 0 .05) ;while the expression level of Th2 transcription factor GATA-3 ,the introcellular secretion of IL-4 in CD4 + T cells and the serum IL-4 were all decreased in patients with SLE combined coronary heart disease(P< 0 .05) .Conclusion There were imbalance towards Th1 cell response in patients with SLE combined coronary heart disease ,which may related to the occurrence and development of disease .

目的 探讨滤泡辅助性T细胞(Tfh)在系统性红斑狼疮(SLE)患者外周血的比例及其临床意义.方法 收集60例SLE患者(SLE组)及60例健康体检者(对照组)外周血,采用流式细胞术方法检测CXCR5+ ICOS+的CD4+T细胞(即Tfh细胞)的比例,并分析Tfh细胞比例与患者疾病活动度及自身抗体的相关性.结果 SLE组患者外周血Tfh细胞比例明显高于对照组(t=5.830,P＜0.01),且SLE活动组明显高于SLE稳定组(t=7.313,P＜0.01),但狼疮肾炎组患者Tfh比例与无肾炎狼疮组比较,差异无统计学意义(t=0.986,P=0.328).SLE患者外周血Tfh细胞比例与疾病活动度、血清总IgG、抗核抗体滴度及抗ds-DNA均呈正相关(P＜0.05).结论 SLE患者外周血Tfh细胞的比例升高可能与患者疾病的发生及发展有关.

<正>原发性胆汁性肝硬化(primarybiliarycirrhosis,PBC)是一种慢性肝内胆汁淤积性疾病,该病的病因未完全明了,可能与免疫异常有关。本病多累及中老年妇女,男女之比为1∶9。PBC的主要临床表现为乏力、黄疸、皮肤瘙痒和血清碱性磷酸酶(alkaline phosphatase,ALP)及γ-谷氨酰转

痛风石是痛风的特征性病变,痛风石形成的典型部位在耳轮,也常见于第一跖趾、指间、踝、腕、膝、肘等处,发生在脊柱部位的痛风石极为少见,我院近期收治1例腰椎痛风石患者,现报道如下: