A novel packaging material (SXS) composed of silk protein peptide (SPP), xanthan gum (XG), and soy protein isolate (SPI) was developed. The incorporation of SPP significantly enhanced the antioxidant activity of the SXS cling film. The aromatic amino acids in SPP and SPI absorbed ultraviolet light, improving the film's UV resistance. Molecular docking verified the interactions among SPI, XG and SPP, indicating multipoint binding that supports the proposed network-strengthening mechanism. The optimum amount of SPP is 6%, providing the best overall balance between UV barrier properties, mechanical strength, and water contact angle. For food preservation, SXS6% reduced the weight loss rate from 6.42% to 4.34%, increased vitamin C (Vc) retention for grape (61% to 79%) and for yellow peaches (51% to 84%). By reducing water evaporation, delaying respiration, and preserving nutrients, the SXS6% film effectively extended the shelf life of fruits, demonstrating its potential for use in food preservation applications.

Food-grade bacterial cellulose (BC) is a promising functional biopolymer for the food industry; however, its productivity is frequently hampered by acid stress in industrially relevant substrates. In this study, we isolate a kombucha-derived strain, Komagataeibacter intermedius FM883, and evaluate its performance in acidic orange juices. Genomic analysis revealed five cellulose synthase operons, versatile carbon utilization pathways, and a robust acid-tolerance system, which collectively facilitate BC biosynthesis under low-pH conditions. Genomebased safety screening against VFDB and CARD databases did not detect known virulence factors or transmissible antibiotic resistance genes, supporting its potential for food-related applications. Consistent with these genomic features, FM883 maintained growth and BC production over a broad acidic range of pH 3.0-6.0. In acidic orange juice, FM883 produced 1.342 +/- 0.092 g/L BC (dry weight) after 7 d of fermentation, while further reducing the pH from 3.35 to 2.78. Structural characterization confirmed that orange juice-derived BC (BC-OJ) retained typical cellulose I alpha features and exhibited the highest crystallinity among the tested carbon sources. These findings establish FM883 as a genome-resolved, acid-tolerant BC-producing bacterium and highlight its potential for sustainable production of BC-enriched acidic beverages and food-grade cellulose biomaterials.

The use of selenium (Se) to enhance the polysaccharide content in Polygonatum cyrtonema Hua (PCH) rhizomes has gained increasing attention as a method to improve their medicinal quality. However, the underlying mechanisms remain largely unexplored, and we investigated these mechanisms by adding selenite [Se(IV)] at concentrations of 0, 1 (Se1), and 10 mg kg-1 (Se10) to the growth medium of PCH in a pot experiment. Our findings revealed that Se addition enhanced PCH growth, photosynthetic efficiency, and rhizome polysaccharide content. Specifically, the Se1 treatment promoted monosaccharide formation, while the Se10 treatment increased the abundance of phosphorylated monosaccharides in PCH rhizomes. Addition of Se generally increased the abundance of metabolites and genes involved in the EMP, TCA, and PPP pathways, thereby influencing carbon partitioning. Compared to the CK treatment, the Se1 treatment altered many polysaccharide characteristics, such as (1) decreasing their molecular weight; (2) increasing the molar ratios of many monosaccharides, except for glucose and galactose; (3) enhancing their hydroxyl radical quenching ability; and (4) altering their surface shapes, molecular structures, and functional groups. This study systematically elucidated the mechanisms by which Se may promote polysaccharide accumulation in PCH rhizomes, offering insights into improving their medicinal quality.

Polygonatum cyrtonema Hua (PCH) is traditionally recognized as both an edible and medicinal food source. Its rhizomes contain numerous bioactive compounds, notably polysaccharides and flavonoids, which serve as key constituents in functional food development. However, the cultivation of PCH is often hindered by allelopathic effects, which diminish its quality and restrict its industrial application. To mitigate these allelopathic influences, three types of biochars derived from maize straw (MB), rice husk (RB), and tea stem (TB) were applied at concentrations of 0%, 2%, and 4%. Initially, the physicochemical properties of these biochars were characterized, followed by an evaluation of their impact on (1) the synthesis of quality-related components, secondary metabolites, and allelochemicals within PCH rhizomes and (2) the fundamental physicochemical properties and bacterial community structure of the PCH rhizosphere soil. The findings indicated that the application of 4% RB significantly enhanced the content of total polysaccharides by 48.5%, total flavonoids by 30.2%, total saponins by 28.6%, and total polyphenols by 18.3%, while concurrently reducing protein (PRO) and free amino acid (FAA) concentrations in the rhizomes. Non-targeted metabolomic analyses revealed that biochar amendments (1) upregulated metabolites involved in the citrate cycle and galactose metabolism pathways, thereby facilitating energy supply and precursors for polysaccharide biosynthesis; (2) downregulated metabolites involved in the arginine biosynthesis pathway, which is unfavorable for protein and amino acid synthesis; (3) decreased the abundance of six identified allelochemicals, including 5-hydroxy-L-tryptophan and andrographolide, with the most pronounced effect observed in the 4% TB treatment (T2); (4) improved soil physicochemical parameters such as pH, soil organic matter (SOM), total nitrogen (TN), and available potassium (AK); and (5) altered the rhizosphere bacterial community by enriching beneficial phyla, notably Myxococcota and Gemmatimonadota. These modifications in soil properties and bacterial community composition were closely associated with enhanced rhizome quality and a reduction in allelochemical accumulation. Collectively, the results of this study elucidate the potential mechanisms linking biochar application to allelopathy mitigation, optimization of soil microbial communities, and improvement of PCH rhizome quality. This research provides a theoretical basis for the production of high-quality PCH while concurrently minimizing allelochemical accumulation in its rhizomes.

Mesona chinensis Benth is a significant botanical resource utilized for both medicinal and dietary purposes, and the Xiaoye variety (XY) exhibited the highest antioxidant activity among the varieties. Despite its importance, metabolic information regarding its medicinal and nutritional properties remains sparse. This study examined the secondary metabolites of four M. chinensis Benth varieties using UHPLC-MS/MS and identified 102, 105, and 286 metabolites exhibiting differential accumulation in the XY variety compared to the Taiwan variety (TW), Minxuan variety (MX), and Zengcheng variety (ZC), respectively, among the 1287 metabolites identified. These metabolites are predominantly involved in secondary metabolic pathways such as “Tropane, Piperidine, and Pyridine Alkaloid Biosynthesis” and “Flavone and Flavonol Biosynthesis”. In addition, we identified the ten most significant differential metabolites that influence antioxidant activity, with flavonoids recognized as the primary contributors to the variation in antioxidant activities. In this study, we have outlined the metabolic landscape of M. chinensis Benth. These findings may aid in elucidating the mechanism behind the antioxidant activity of XY, which provides valuable insights for breeding, quality assurance, and product innovation related to M. chinensis Benth.

Microbial contamination challenges have led to the development of active edible coatings for fruit preservation. Herein, a Konjac glucomannan (KGM) coating loaded with Ocimum gratissimum (OG) essential oil stabilized by pectin with superior resistance to air permeability, oxidation, and fungal, was prepared in situ on the surface of Mandarin oranges to enhance postharvest fruit quality. The results demonstrated that the KGM-pectin-OG (K-P-OG) 1.5 wt% coating exhibited good performance in terms of stability, adhesion, and wetting. Meanwhile, the coating had an ideal air permeability due to its compact and dense structure based on the good compatibility and interactions between the components. The oxygen permeability of the K-P-OG coating was 7.9 × (10-16 g·cm)/(cm2·s·Pa), which was six orders of magnitude lower than that of the KGM coating. The antioxidant, in vitro, and in vivo antifungal activities against Penicillium italicum of the coating were strengthened by the OG emulsion and mainly depended on its concentration. The storage results showed that the K-P-OG 1.5% coating extended the shelf life of Mandarin oranges by 8 days, reduced the weight loss rate by 13%, and increased the firmness and POD during storage by 24.14% and 100%, respectively, compared with the control group. These results demonstrate that K-P-OG can effectively maintain nutrient content and extend the storage time of Mandarin oranges by enhancing antioxidant capacity and inhibiting fruit respiration and microorganism growth. This study presents a strategy for developing edible coatings for postharvest fruit preservation.

IntroductionProlonged monoculture cultivation of Paris polyphylla has been linked to challenges such as disruption of rhizosphere soil microbial balance and deterioration in crop quality. Intercropping has emerged as a viable strategy to address these issues.MethodsIn the present study, monoculture of P. polyphylla (PP) served as the control, while two intercropping systems were implemented: P. polyphylla with Polygonatum cyrtonema (PPPC) and P. polyphylla with Ganoderma lucidum (PPG). The objective was to evaluate the P. polyphylla quality, soil physicochemical properties, and the structure of the microbial community.ResultsFindings revealed that the PPG intercropping system significantly increased available potassium levels by 50.28% and enhanced the abundance of Trichoderma by 3,022% via the G. lucidum network. These alterations were associated with improvements in P. polyphylla yield (51.30%), polyphyllin VII content (34.16%), and total polyphyllin content (30.59%). Conversely, the PPPC system promoted the enrichment of Cupriavidus and nitrogen-fixing bacteria such as Hyphomicrobiales, leading to a 26.78% rise in available phosphorus and a 20.00% increase in polyphyllin II content in P. polyphylla. Both intercropping approaches markedly elevated the abundance of Basidiomycota, with the PPPC system further enriching functional microbial taxa including Glomeromycota and Saitozyma.DiscussionCollectively, these results demonstrate that intercropping P. polyphylla with either P. cyrtonema (PPPC) or G. lucidum (PPG) enhances soil physicochemical attributes, optimizes the composition of rhizosphere microbial communities, and positively influences the accumulation and yield of bioactive compounds.

IntroductionPseudostellaria heterophylla (PSH) is a renowned medicinal and culinary plant. However, soil-related factors limiting in the improvement of its yield and quality in practice remain poorly understood.MethodsWe sampled two PSH varieties and their associated rhizosphere soils from two sites in Ningde City, Fujian Province, China, including Chouling village (Z) and Wuyang village (W), to analyze the basic soil physicochemical properties, soil bacterial communities, and untargeted metabolomics of both soils and tuberous roots samples. ResultsOur results showed that the rhizosphere soil at site Z had significantly higher concentrations of calcium (Ca), magnesium (Mg), manganese (Mn), available phosphorus (AP), and available potassium (AK), but a lower concentration of alkaline hydrolyzable nitrogen (AN). Soil AN and AK were identified as key determinants of bacterial community structure, showing negative and positive correlation with numerous microbial phyla and genera, respectively. Site Z exhibited higher abundances of functional bacterial phyla, including Desulfobacterota, Nitrospirota, and Elusimicrobiota, as well as dominant genera of Acidobacteriota. Amino acids and peptides (AAs) were the most abundant class of differential metabolites (DMs) in both the rhizosphere soil and tuberous roots of PSH. The accumulation of AAs in tuberous roots was positively correlated with soil pH, electrical conductivity (EC), and potassium (K) levels, but negatively correlated with AN. Furthermore, specific microbial taxa and soil DMs at site Z were positively associated with AA abundance in the tuberous roots. DiscussionThese findings suggest that appropriate soil nitrogen levels coupled with relatively high potassium availability, pH, and EC conditions are conducive to AAs accumulation in PSH tuberous roots.

The production of Polygonatum cyrtonema Hua is currently encountering substantial challenges due to root rot disease, primarily caused by the pathogen Fusarium oxysporum. The WRKY transcription factors play crucial roles in mediating disease resistance across a wide range of plant species; however, it is unclear whether members of this family improve resistance to F. oxysporum infection in P. cyrtonema Hua. To putatively determine the F. oxysporum responsive PcWRKY members, we analyzed full-length transcriptome sequencing data obtained after F. oxysporum infection in this study, 271 WRKY transcription factors were detected and PcWRKY33 was found to be responsive to F. oxysporum infection. Furthermore, overexpression of PcWRKY33 in both P. cyrtonema Hua and Nicotiana benthamiana increased tolerance to F. oxysporum infection, and the expression level of 20 disease-resistance genes containing the TGAC motif were regulated using transcriptome sequencing analysis post-inoculation with F. oxysporum in PcWRKY33 overexpression seedlings. We also determined that F. oxysporum infection increased both the endogenous salicylic acid (SA) content and the expression of disease-resistant genes in PcWRKY33-overexpressing plants. Our results demonstrated that PcWRKY33 plays an important role in the resistance of P. cyrtonema Hua to F. oxysporum by influencing SA signaling.

Colletotrichum gloeosporioides, the primary fungal pathogen responsible for mango anthracnose, causes significant economic losses. Hence, we developed a xanthan gum/hydroxypropyl methylcellulose (XG/HPMC) film incorporating Dictyophora echinovolvata carbon quantum dots (DE-CQDs). These green-synthesized DE-CQDs are 2 nm spherical nanoparticles with good crystallinity and thermal stability, featuring functional groups such as -OH, -COOH, -NH₄+, and -SO₃H, which enhance hydrophilicity, solubility, and charge properties. The negative charge of DE-CQDs, as evidenced by zeta potential measurements, enhances their dispersibility and inhibits aggregation, thereby contributing to the film's augmented UV-blocking and water vapor transmission characteristics. DE-CQDs inhibit C. gloeosporioides growth through a unique mechanism, inducing metacaspase-dependent apoptosis and causing ROS accumulation without DNA fragmentation. When incorporated into the XHC film, DE-CQDs improve UV-blocking by 30 % at 400 nm, enhance water vapor transmission, and increase water contact angle. The XHC film significantly reduces mango decay (decay index: 0.35 ± 0.05 to 0.21 ± 0.05) and boosts vitamin C retention (from 30 % to 61 % over 7 days), effectively extending shelf life and preserving nutritional quality. Among the XHC films, XHC6 shows the best overall performance. Thus, DE-CQDs serve as functional fillers in biodegradable, environmentally friendly packaging films, offering a novel solution for mango disease prevention and control.

Zengcheng (ZC), a superior cultivar of Mesona chinensis Benth, is distinguished by its unique sensory attributes and exceptional phytochemical quality, rendering it the cultivar of choice for commercial production. In this investigation, we employed comprehensive targeted metabolomics to systematically delineate the volatile metabolome of ZC in comparison to three relevant counterparts grown under uniform field conditions and harvested at the same developmental stage. Our primary objectives were to (i) quantitatively characterize the metabolomic divergence between ZC and the comparative cultivars, and (ii) elucidate the molecular basis underlying ZC's characteristic flavor signature. Through high-throughput gas chromatography-mass spectrometry (GC-MS) analysis, a total of 1767 volatile metabolites were confidently annotated, with terpenoids and esters identified as the predominant chemical classes. Notably, ZC exhibited significantly elevated levels of terpenoids, esters, and aldehydes, which collectively conferred an intensified green, sweet, herbal, and fruity aroma. Key discriminatory metabolites included geranyl formate and geranic acid (imparting a pronounced green note), which were identified as pivotal determinants of ZC's unique flavor profile. These findings provide mechanistic insights into the biochemical pathways governing flavor development in M. chinensis Benth and establish a robust foundation for precision-breeding strategies aimed at enhancing sensory quality and developing elite cultivars.

Loquat leaf extract (LLE) was added to guar gum and pullulan as an environmentally friendly packaging film (GPE) to preserve Chinese water chestnuts (CWCs). The effect of the amount of LLE on the guar gum/pullulan composite film was investigated. The optimal amount of LLE was 4% (GPE4), with lower water vapor permeability (WVP) and greater mechanical strength, antioxidant, and comparable antibacterial performance than many pullulan-based films. Upon packing the CWCs for 4 days, the weight loss rate of GPE4 was only 1.80 ± 0.05%. For GPE4, the POD activity, the soluble solid content, and the vitamin C (Vc) content of the CWCs were 21.61%, 36.16%, and 26.22% higher than those of the control sample, respectively. More importantly, GPE4 was effective in preserving the quality of CWCs after 4 days of storage, better or at least comparable to non-biodegradable plastic wrapping (PE). Therefore, it can be concluded that GPE films hold significant promise as a sustainable alternative packaging material for preserving fruit-based foods like CWCs, potentially replacing PE in the future.

Pullulan-based composite film can be a potential alternative packing material to non-environmentally friendly plastic wrap (PE) to preserve fresh-cut carrots. However, many developed pullulan-based composites either have high water vapor permeability (WVP) and high mechanical strength or vice versa, which limits the practicality of the developed packaging materials for potential commercialization. Herein, Abelmoschus manihot gum (AMG)/ pullulan/magnesium L-ascorbate (MLA) was created as a green composite film (APL) to preserve fresh-cut carrots. The optimal amount of MLA was found to be 10 % (APL10), demonstrating a balance of lower WVP and greater mechanical strength and antioxidant performance than many pullulan-based films. This effectively solved many problems faced by other pullulan-based packaging films. After the fresh-cut carrots were packed with the composite film for 4 days, it was found that APL10 was effective in preserving the quality of carrots, in terms of freshness, weight loss rate, Vitamin C (VC), and malondialdehyde (MDA) content after 4 days of storage, much better than non-biodegradable PE. Thus, based on these findings, it is concluded that APL films have huge potential as a green packaging material for food to replace PE in the future.

Bacillus thuringiensis Cry2Ab toxin was a widely used bioinsecticide to control lepidopteran pests all over the world. In the present study, engineering of Bacillus thuringiensis Cry2Ab toxin was performed for improved insecticidal activity using site-specific saturation mutation. Variants L183I were screened with lower LC 50 (0.129 µg/cm 2 ) against P. xylostella when compared to wild-type Cry2Ab (0.267 µg/cm 2 ). To investigate the molecular mechanism behind the enhanced activity of variant L183I, the activation, oligomerization and pore-formation activities of L183I were evaluated, using wild-type Cry2Ab as a control. The results demonstrated that the proteolytic activation of L183I was the same as that of wild-type Cry2Ab. However, variant L183I displayed higher oligomerization and pore-formation activities, which was consistence with its increased insecticidal activity. The current study demonstrated that the insecticidal activity of Cry2Ab toxin could be assessed using oligomerization and pore-formation activities, and the screened variant L183I with improved activity might contribute to Cry2Ab toxin’s future application.

A novel film composed of Polygonatum cyrtonema extracts (PCE), xanthan gum (XG), flaxseed gum (FG) and carboxymethyl cellulose (CMC) was prepared (XFCP). Addition of PCE has decreased the light transmittance, while enhanced the UV blocking performance, antioxidant activity, tensile strength and elongation at break of XFCP due to polysaccharides, polyphenols, and flavonoid in PCE. Structural analyses by FTIR and XRD indicated the hydrogen-bonding interaction between PCE, XG, FG and CMC. It was found that compared with the control sample, XFCP2.5% with the lowest WVTR was able to prolong the shelf life of mango. The overall quality of mango was also improved in terms of lower decay rate, weight loss rate, total soluble solid, and polyphenol oxidase, higher titratable acidity, Vc, and superoxide dismutase than control mango upon 8 days of storage. This effectively expanded the application of PCE into food packaging in addition to merely as Chinese traditional medicine herbs.

为分析我国13个省份博落回种质资源的遗传多样性,明确其亲缘关系和遗传差异.利用SSR荧光标记毛细管电泳技术对16份博落回种质资源进行遗传多样性分析.结果表明:16个博落回种质资源多态信息含量(PIC)值的变化范围为0.375～0.687,遗传相似系数范围为0.071 4～0.7142.聚类分析结果显示,在相似系数0.3处可将16份博落回种质资源分为3个大类群,第Ⅰ大类有7份种质资源,第Ⅱ大类有1份种质资源,第Ⅲ大类有8份种质资源.此外,构建了 16份博落回种质资源的DNA指纹图谱数据库,开发了相对应的指纹图谱.研究结果可为博落回种质资源的分类鉴定、遗传多样性分析和品种选育提供可用的分子标记以及参考依据.

在福建省三明市明溪县博落回种植区发现疑似炭疽病病例,为明确福建三明地区博落回叶片炭疽病致病的病原菌,对采集的典型病样进行病原菌的分离,并采用形态学、分子生物学和致病性测定对病原进行鉴定.结果表明:从博落回病叶中共分离到20株菌株,所有菌株的菌落形态均一致,菌落圆形,白色,背面浅灰白色至肉桂色,分生孢子团橘红色,分生孢子为长椭圆形,单孢;ITS和TUB2基因系统发育树显示,供试的菌株和果生炭疽菌Colletotrichum fructicola均聚类在一起,置信度分别为82％和99％;致病性测定结果表明,发病叶片的症状与田间的一致,符合柯赫氏法则.通过病样发病症状、菌落形态及基因序列(ITS和TUB2)分析,发现引起三明市明溪县的博落回叶片炭疽病病原菌为果生炭疽菌C.fructicola.

The flower volatile compounds of Jasminum spp.were detected by using the SPME-GC-MS technique and diversity of flower volatile compounds and aroma quality were also evaluated by the cluster analysis,principal component analysis and aroma quality evaluation.The results showed that the 25 jasmine germplasm resources displayed abundant phenotypic traits:(i)the phyllotaxy of these jasmine germplasm resources was verticillate three-leaves,alternately pinnate compound leaves or opposite single-leaf;(ii)the flower color is white,white-pink bicolor or golden;and(iii)the corollas is single,double or multiple layer(s).Among the 25 germplasm resources,the lightest single flower mass was Jasminum grandiflorum(0.10 g),and the heaviest one was Jasminum sambac Hutou(2.41 g).Moreover,a total of 107 volatile components were identified from the 25 jasmine germplasm resources,including 43 Terpenoids,24 benzene compounds,28 fatty acid derivatives and 12 N-and NS-containing compounds.The volatile components of 22 germplasm resources were reported for the first time by this study,except for J.grandiflorum,J.multiflorum and Fuzhou double layers J.sambac.According to the results of the principal component and cluster analyses,the 25 jasmine germplasm resources could be clustered into three categories:Group Ⅰ,including J.odoratissimum;Group Ⅱ,including J.seguinii,J.multiflorum and J.dichotomum;and Group Ⅲ,including the remaining 21 germplasms.Furthermore,21 substances including linalool,benzyl alcohol,methyl benzoate,benzyl acetate,α-farnesene,indole,jasmone and butyraldehyde oxime were screened as the differentially representative volatile components.In J.odoratissimum,few volatile components could be identified,but the content of α-farnesene was very high(＞89％).Butyraldehyde oxime-like substances were volatile components unique to group Ⅱ,and have not been detected in other jasmine germplasm resources.J.grandiflorum had significantly different phenotypic characteristics compared to J.Jasmine,while its volatile compositions were similar to J.Jasmine.The results of aroma quality evaluation indicated that aroma quality of different cultivars of J.sambac was significantly different.It is noteworthy that the aroma quality of Orleans girl,Myanmar double layers,Thailand double layers and Taiwan single layer was not lower than that of Fuzhou's double layers,therefore,it is worth planting in a large area.

Grape seed extract (GSE) was added to pullulan polysaccharide (PP)/xanthan gum (XG) as composite film (PP/ XG/GSE or PXG). The observed composite morphology indicated their biocompatibility. Sample PXG100 (contain 100 mg/L GSE) demonstrated the best mechanical properties, with tensile strength of 16.62 +/- 1.27 MPa, and the elongation at break of (22.60 +/- 0.48)%. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activity of PXG150 were the highest at (81.52 +/- 1.57)% and (90.85 +/- 1.54)%, respectively. PXG films also demonstrated inhibitory effects on Staph-ylococcus aureus, Escherichia coli, and Bacillus subtilis. The PXG films could also prolong the shelf life of fresh-cut apples because it could decrease the rate of weight loss and retain more vitamin C and total polyphenol even on the 5th day. The weight loss rate of PXG150 was decreased from (8.58 +/- 0.6)% (control) to (4.15 +/- 0.19)%. It was able to achieve vitamin C and total polyphenol retention rate of 91 % and 72 %, respectively, which was significantly higher that the control sample. Therefore, GSE had contributed in enhancing the antibacterial, antioxidant properties, mechanical strength, UV protection and water resistance in PXG composite films. This effectively extend the shelf life of fresh-cut apples, which it will be an excellent food packaging material.

[目的]探究不同致病力青枯雷尔氏菌诱导番茄防御相关基因的表达水平,为探明无致病力青枯雷尔氏菌的生防机制奠定基础.[方法]以无菌水为对照(CK),分别接种青枯雷尔氏菌强致病力菌株FJAT91和无致病力菌株FJAT1458,接种后不同时间(0,3,6,12,24,36,48,72 h)取样,利用荧光定量PCR技术,检测接种不同致病力青枯雷尔氏菌后番茄植株中水杨酸(SA)信号转导途径的gluA和PR-1a基因、茉莉酸(JA)信号转导途径的loxA和pin2基因及乙烯(ET)信号转导途径的Osm和PR-1a基因相对表达量的变化.[结果]菌株FJAT1458和FJAT91均能诱导番茄gluA、PR-1a、loxA、pin2、Osm和PR-1a基因的表达,接种后期(36 h之后),这些基因(PR-1a和Osm除外)在FJAT1458处理组的表达量均比FJAT91处理组高;FJAT1458和FJAT91诱导gluA和PR-1A基因表达量呈"先上升后下降"的趋势,接种12 h表达量最大;FJAT1458诱导番茄植株loxA和pin2基因表达量均显著高于CK,FJAT91诱导番茄植株loxA基因和pin2基因表达量在接种初期显著高于CK和FJAT1458处理组,后期迅速降低;FJAT-91诱导番茄植株Osm基因显著高于FJAT1458和对照处理(72 h除外),该菌株诱导番茄植株PR-1b基因表达量变化较为强烈,接种3 h,其诱导的PR-1b基因表达量为CK的251.33倍.[结论]不同致病力青枯雷尔氏菌诱导番茄防御相关基因表达量不同,可以进一步通过转录组法挖掘更多差异表达的基因.