为人民身体健康提供医疗和护理保健服务。医疗与护理、医学教学和培训、医学科研、卫生防疫、妇女保健与健康教育、学术交流等。
Objective To explore and analysis the effect of internal heat acupuncture combined with platelet rich plasma(PRP)on T cell subsets in peripheral blood of patients with knee osteoarthritis(KOA).Methods From May 2020 to October 2023,84 cases of pa-tients with knee osteoarthritis treated at a certain hospital were selected as the research subjects for this study.According to the principle of random coin tossing,the 84 cases of patients were divided into an experimental group of 42 cases and a traditional group of 42 cases.The traditional group were treated with intra-articular injection of sodium hyaluronate,while the experimental group were treated with in-ternal heat acupuncture combined with PRP.The treatment were observed for 6 weeks.Recorded the overall efficacy,and changes of vis-ual analogue scale(VAS),serum interleukin-17(IL-17)levels,T cell subsets in peripheral blood of two groups.Results After 6 weeks of treatment,the total effective rate of the experimental group were 97.62%,while that of the traditional group were 83.33%.The experimental group showed a significant improvement compared to the traditional group(P<0.05).The pain VAS scores of the experi-mental group and the traditional group after 6 weeks of treatment were lower than before treatment(P<0.05),and the pain VAS scores of the experimental group decreased more compared to the traditional group after 6 weeks of treatment(P<0.05).After6 weeksoftreat-ment,the serum IL-17 levels in the experimental group and the traditional group were significantly lower than before treatment(P<0.05).After 6 weeks of treatment,the serum IL-17 levels in the experimental group were significantly lower than those in the tra-ditional group(P<0.05).After 6 weeks of treatment,the relative proportion of CD4+T cells in peripheral blood of the experimental group and the traditional group were lower than before treatment(P<0.05),and the relative proportion of CD8+T cells were higher than before treatment(P<0.05).After 6 weeks of treatment,the relative proportion of CD4+T cells and CD8+T cells in peripheral blood of the experimental group were also statistically significant compared to the traditional group(P<0.05).Conclusion The application of in-ternal heat acupuncture combined with PRP in patients with knee osteoarthritis can improve the treatment effect,promote pain relief,in-hibit serum IL-17 expression,regulate the proportion of T cell subsets in peripheral blood,and continuously improve the prognosis of patients.
Objective To investigate the effect of Parkin protein on aerobic glycolysis and apoptosis of breast cancer MDAMB-231 cells. Methods Western blot was firstly used to detect the expression level of Parkin in normal breast epithelial cells MCF-10A and breast cancer cells MDA-MB-231. Then MDA-MB-231 cells transfected with Parkin overexpression plasmids were used as the experimental group, and the MDA-MB-231 cells transfected with empty vectors as the control group. Glucose consumption, lactic acid, and ATP production of these two groups were detected by those related kits, respec-tively. The expression of glycolysis related proteins including GLUT1, PKM2, and LDHA was evaluated by Western blot. Cell growth, colony formation, and wound healing migration assays were used to evaluate the ability of cell proliferation and migration for these two groups. The expression of apoptosis-related proteins Bax, Bcl-2, and cleaved Caspase-3 were examined by Western blot. Results Compared with normal breast cells, the expression of Parkin protein in MDA-MB-231 cells decreased significantly（P＜0.01）. The results showed that high expression of Parkin led to decreased production of glucose uptake and lactic acid production and increased level of ATP（P＜0.05）. Compared to the control group, the expression of glycolysis-related proteins GLUT1, PKM2, and LDHA decreased in the Parkin group（P＜0.05）. Overexpression of Parkin in MDAMB-231 cells reduced the cell proliferation and migration. Moreover, the expression of anti-apoptotic protein Bcl-2 de-creased and the expression of cleaved Caspase-3 increased（P＜0.05）. Conclusion The expression of Parkin in MDA-MB-231cells is lower than that in MCF-10A cells. The high expression of Parkin leads to the inhibition of aerobic glycolysis pathway,the decrease of proliferation and migration ability, and apoptosis of MDA-MB-231 cells.
