为妇女儿童心身健康提供服务。开展妇产科:妇科、产科、计划生育、优生学;妇女保健:青春期保健、围产期保健、更年期保健、妇女心理卫生、妇女营养;儿科:新生儿、小儿消化、小儿呼吸、小儿心脏病、小儿肾病、小儿血液病;儿童保健:儿童生长发育、儿童营养、儿童心理卫生、儿童五官保健、儿童康复。急诊医学、康复医学、麻醉、医学检验、医学影像、中医、病理、预防保健。开展内、外、皮肤等门诊服务。妇女病普查、遗传病筛查、产前诊断与接生、高危孕产妇筛查、监测与监护高危新生儿筛查、治疗与监护儿童疾病防治、妇幼卫生监测与信息管理、妇幼卫生保健人员培训。
Objective:To study the epidemiological characteristics and pathogen status of infection of patients with herpetic pharyngitis in eastern region of Chengdu in 2022, in order to provide experimental basis for scientific prevention and control of herpetic pharyngitis.Methods:Information and fecal specimens were collected from patients diagnosed with herpetic angina at sentinel monitoring hospitals in eastern region of Chengdu from January to December 2022, their epidemiological characteristics were analyzed, real-time fluorescence quantitative PCR (RT-PCR) and semi nested RT-PCR (RT-snPCR) were used to detect and identify enterovirus subtypes.Results:Among the 236 cases of herpetic angina, the age of the affected children was mainly 0-9 years (99.15%), with children aged below 3 years accounting for 75.85% of the total cases, children in the 1-year-old group had the highest constituent ratio, accounting for 47.88% of the total cases; The virus detection rate in the 2-year-old group was higher than that in the 0-year-old group ( χ2=5.945, P=0.015). There were 142 male infected cases (60.17%) and 94 female cases (39.83%); there was a statistically significant difference in the virus detection rate between the male group (91.55%) and the female group (78.72%) ( χ2=7.938, P=0.005). In the seasonal distribution, the summer group had the highest constituent ratio (40.25%), while the winter group had the lowest constituent ratio (11.86%), The composition ratio of the summer group was significantly higher than that of the other groups, and the differences were statistically significant ( χ2=10.393, 19.199, 49.358, all P<0.001). The virus detection rate in the summer group was significantly higher than that in the spring group and the winter group ( χ2=16.951, 4.592, both P<0.05). The RT-PCR result showed that a total of 204 out of 236 children with herpetic angina were virus positive, with a detection rate of 86.44% (204/236), including coxsackievirus A (CV-A) 6 (22.55%), CV-A16 (5.88%), CV-A2 (2.94%), enterovirus type A71 (EV-A71) (2.94%), and other enteroviruses (65.69%). The RT-snPCR result showed that five viruses were identified from other enteroviruses, including CV-A4 (8.33%), CV-A5 (6.86%), CV-A10 (12.25%), CV-A12 (24.02%), and coxsackievirus B3 (CV-B3) (6.86%); another 15 positive samples of enterovirus were not successfully classified. We selected 9 strains of the virus in this study and 30 reference sequences from the NCBI database to. Conclusions:Herpetic angina in eastern region of Chengdu in 2022 is mainly found in male children under 3 years of age, and was concentrated between April and June. It has been confirmed that CV-A12 and CV-A6 were more common in intestinal viruses, and the 9 strains isolated in this study were homologous to multiple isolates.
Objective To investigate the correlation between T-cell subsets and airway damage in protracted bacterial bronchitis（PBB）.Methods From June 2019 to January 2022,112 children with PBB（PBB group）,112 children without PBB（non-PBB group） and 112 healthy children with physical examination（control group） who visited Longquanyi District of Maternal and Child Health Care Hospital were selected as subjects.The levels of CD3 + T cells, CD4 + T cells, CD8 + T cells, CD4 + /CD8 + in children with different airway damage levels in each group were compared, as well as the risk of airway damage in children with different levels of T cell subsets.Spearman was used to analyze the relationship between T cell subsets and the degree of airway damage of PBB,multivariate Logistic regression equation was used to analyze the related influencing factors of airway damage of PBB,and receiver operating characteristic（ROC） curve and area under ROC curve（AUC） were used to analyze the value of T cell subsets in evaluating airway damage of PBB.Results There were statistically significant differences in CD3 + T cells, CD4 + T cells, CD8 + T cells and CD4 + /CD8 + in three groups（P＜0.05）.Compared with those without airway damage, CD3 + T cells and CD8 + T cells were decreased in mild, moderate and severe children with airway damage, while CD4 + T cells and CD4 + /CD8 + were increased, and the pairwise differences were statistically significant（P＜0.05）.Patients with abnormal CD3 + T cells, CD4 + T cells, CD8 + T cells and CD4 + /CD8 + cells had a higher risk of airway damage than normal patients（P＜0.05）.CD3 + T cells and CD8 + T cells negatively correlated with airway damage of PBB（P＜0.05）,while CD4 + T cells and CD4 + /CD8 + positively correlated with airway damage of PBB（P＜0.05）.Multivariate Logistic regression analysis showed that CD3 + T cells, CD4 + T cells, CD8 + T cells, CD4 + /CD8 + significantly correlated with airway damage of PBB（P＜0.05）.The AUCs of CD3 + T cells, CD4 + T cells, CD8 + T cells, CD4 + /CD8 + in the assessment of airway damage of PBB were 0.783,0.751,0.764,0.804,respectively, and the AUC of the combined evaluation of the indexes was 0.892（P＜0.05）.Conclusion The disorder of T-cell subsets in children with PBB is closely related to airway damage and its severity.The combined detection of the four indicators is expected to provide a non-invasive and convenient means for the assessment of airway damage in children with PBB,and provide a new idea for clinical prevention and treatment of PBB and airway damage.
