Objective:To study the epidemiological characteristics and pathogen status of infection of patients with herpetic pharyngitis in eastern region of Chengdu in 2022, in order to provide experimental basis for scientific prevention and control of herpetic pharyngitis.Methods:Information and fecal specimens were collected from patients diagnosed with herpetic angina at sentinel monitoring hospitals in eastern region of Chengdu from January to December 2022, their epidemiological characteristics were analyzed, real-time fluorescence quantitative PCR (RT-PCR) and semi nested RT-PCR (RT-snPCR) were used to detect and identify enterovirus subtypes.Results:Among the 236 cases of herpetic angina, the age of the affected children was mainly 0-9 years (99.15%), with children aged below 3 years accounting for 75.85% of the total cases, children in the 1-year-old group had the highest constituent ratio, accounting for 47.88% of the total cases; The virus detection rate in the 2-year-old group was higher than that in the 0-year-old group ( χ2=5.945, P=0.015). There were 142 male infected cases (60.17%) and 94 female cases (39.83%); there was a statistically significant difference in the virus detection rate between the male group (91.55%) and the female group (78.72%) ( χ2=7.938, P=0.005). In the seasonal distribution, the summer group had the highest constituent ratio (40.25%), while the winter group had the lowest constituent ratio (11.86%), The composition ratio of the summer group was significantly higher than that of the other groups, and the differences were statistically significant ( χ2=10.393, 19.199, 49.358, all P<0.001). The virus detection rate in the summer group was significantly higher than that in the spring group and the winter group ( χ2=16.951, 4.592, both P<0.05). The RT-PCR result showed that a total of 204 out of 236 children with herpetic angina were virus positive, with a detection rate of 86.44% (204/236), including coxsackievirus A (CV-A) 6 (22.55%), CV-A16 (5.88%), CV-A2 (2.94%), enterovirus type A71 (EV-A71) (2.94%), and other enteroviruses (65.69%). The RT-snPCR result showed that five viruses were identified from other enteroviruses, including CV-A4 (8.33%), CV-A5 (6.86%), CV-A10 (12.25%), CV-A12 (24.02%), and coxsackievirus B3 (CV-B3) (6.86%); another 15 positive samples of enterovirus were not successfully classified. We selected 9 strains of the virus in this study and 30 reference sequences from the NCBI database to. Conclusions:Herpetic angina in eastern region of Chengdu in 2022 is mainly found in male children under 3 years of age, and was concentrated between April and June. It has been confirmed that CV-A12 and CV-A6 were more common in intestinal viruses, and the 9 strains isolated in this study were homologous to multiple isolates.

目的 探讨缺氧诱导因子(HIF)介导的包括DEP结构域含有雷帕霉素靶蛋白相互作用蛋白(DEPTOR)通过程序性细胞死亡4(PDCD4)/c-Jun(AP-1)信号通路参与肝癌HepG2细胞侵袭和血管生成的过程.方法 对数生长期的HepG2细胞侵分为3组-空白组、对照组与HIF组,对照组与HIF组分别转染HIF反义RNA-2对照与HIF反义RNA-2各1μg,空白组以等剂量的磷酸盐缓冲液代替.细胞转染后24 h与36 h,采用四甲基偶氮唑盐微量酶反应比色法(MTT法)实验检测细胞增殖,Transwell小室检测细胞侵袭,流式细胞仪检测细胞凋亡,免疫印迹法(Western blot)检测蛋白表达.结果 细胞转染后24 h与36 h,HIF组的细胞增殖指数、侵袭指数均显著低于空白组与对照组,差异有统计学意义(P<0.05),细胞凋亡指数均显著高于空白组与对照组,差异有统计学意义(P<0.05),而空白组与对照组比较,差异无统计学意义(P>0.05).细胞转染后24 h与36 h,HIF组的DEPTOR蛋白相对表达水平显著低于空白组与对照组,PDCD4与c-Jun(AP-1)蛋白水平显著高于空白组与对照组,差异有统计学意义(P<0.05),空白组与对照组比较,差异无统计学意义(P>0.05).结论 抑制HIF的表达能促进肝癌细胞凋亡,抑制细胞增殖与侵袭,能够介导DEPTOR蛋白的表达,从而促进PDCD4/c-Jun(AP-1)通路的激活与抑制血管生成.

目的 探讨外周血细胞形态检测在血液系统疾病诊断中的应用价值.方法 选取2019年1月～2019年10月血液细胞分析仪检测出结果异常或有报警信息的标本,共计550例,再进行显微镜人工镜检.结果 550例结果异常或有报警信息提示的标本共筛查出27例血液系统疾病.27例血液系统疾病中,急性粒细胞白血病3例11.1％、急性早幼粒细胞白血病2例7.41％、慢性粒细胞白血病2例7.41％、骨髓纤维化1例3.70％、慢性淋巴细胞白血病2例7.41％、缺铁性贫血6例22.22％、骨髓增生异常综合征3例11.1％、巨幼细胞贫血4例(14.81％)、再生障碍性贫血1例3.70％、毛细胞白血病1例3.70％、多发性骨髓瘤1例3.70％、急性淋巴细胞白血病1例(3.70％).结论 当发现血细胞分析仪检测结果异常或有报警信息提示的标本,一定要按照复检规则进行涂片镜检,通过细胞形态学变化及时发现某些血液系统疾病,并报告临床医生及时采取下一步检查,避免误诊、漏诊.

目的 分析血浆抗凝血酶Ⅲ(antithrombin,AT-Ⅲ)、D-二聚体(D-Dimer)、 纤维蛋白原降解产物(fibrinogen degradation product,FDP)对静脉血栓栓塞症(venous thromboembolism,VTE)的诊断和治疗价值.方法 选取2017年1月—2018年4月收治的80例VTE患者(包括71例深静脉血栓和9例肺栓塞)为研究对象,选取同期50例健康人群作为对照组,均检测血浆AT-Ⅲ、D-Dimer、FDP含量水平,对深静脉血栓和肺栓塞患者进行规范治疗,比较健康对照组、深静脉血栓组、 肺栓塞组血浆AT-Ⅲ、D-Dimer、FDP含量水平;比较治疗前、治疗24h后、治疗1周后深静脉血栓组、肺栓塞组的AT-Ⅲ、D-Dimer、FDP含量水平变化;比较VTE患者不同预后(存活/死亡)血浆AT-Ⅲ、D-Dimer、FDP含量水平变化.结果 D-Dimer、FDP含量水平,肺栓塞组和深静脉血栓形成组显著高于对照组,且肺栓塞组高于深静脉血栓形成组(P<0.05);AT-Ⅲ活性,肺栓塞组和深静脉血栓形成组显低于照组,且肺栓塞组低于深静脉血栓形成组(P<0.05).存活组在D-Dimer、FDP含量上显著低于死亡组,AT-Ⅲ活性高于死亡组,差异有统计学意义(P<0.05).深静脉血栓形成组、肺栓塞组治疗24 h后、治疗1周后较治疗前血浆D-Dimer、FDP水平均显著下降(P<0.05),且随治疗时间延长,其水平下降更加显著;AT-Ⅲ活性较治疗前明显上升,且随着治疗时间延长,其活性逐渐升高.结论 血浆AT-Ⅲ、D-Dimer、FDP联合检测对VTE临床诊断有重要的指导意义,且对其病情评估也有重要的参考价值,D-Dimer、FDP含量平稳下降和AT-Ⅲ活性增加在一定程度上提示预后良好.

目的 探讨血清肿瘤标志物CA153和CA125在肺癌患者诊断中的应用价值.方法 选取2015年2月至2018年2月间四川省资阳市第四人民医院收治的30例肺癌患者为肺癌组,另选取同期30例肺部良性病变患者为良性组,采用电化学发光法对两组患者血清CA153和CA125水平进行检测.比较两组患者CA153和CA125阳性情况,CA153和CA125单独与联合检测在肺癌诊断中的价值,比较肺癌组不同病理类型患者的血清CA153和CA125水平.结果 肺癌组患者肿瘤标志物CA153和CA125的阳性率分别为50.0％(15例)和83.3％(25例),均高于良性组的26.7％(8例)和13.3％(4例),差异均有统计学意义(均P＜0.05).鳞癌和腺癌患者血清CA125水平均高于小细胞癌患者,而鳞癌患者又高于腺癌患者,差异均有统计学意义(均P＜ 0.05).血清CA153和CA125联合检测在肺癌诊断中的灵敏度和正确诊断指数均高于CA153和CA125单独检测,差异均有统计学意义(均P ＜0.05).结论 血清肿瘤标志物CA153和CA125联合检测在肺癌诊断中的应用价值较单独检测高,值得临床推广.

目的:探讨Sysmex XE 5000全自动血液分析仪计数外周血有核红细胞(NRBC)的临床应用价值.方法:应用Sysmex XE 5000血液分析仪对105例外周血标本进行NRBC计数,同时对同一份外周血标本的有核红细胞采用人工显微镜镜检进行百分率计数.以镜检法作为参考标准,比较不同方法的计数结果.结果:Sysmex XE5000血液分析仪与人工显微镜镜检计数外周血中NRBC结果有很好的相关性(r=0.975)且两者差异无统计学意义(P>0.05).结论:Sysmex XE 5000全自动血液分析仪能快速、准确地自动计数外周血有核红细胞,可以作为外周血有核红细胞计数的一种过筛检测方法.

目的 探讨非小细胞肺癌患者血清白蛋白(ALB)、前白蛋白(PAB)水平与非小细胞肺癌预后的关系.方法 选取2011年8月至2013年3月就诊于我院确诊的非小细胞肺癌患者210例,选择60例肺部良性疾病患者作为对照,采用生化分析仪检测NSCLC患者和良性组患者血清ALB、PAB表达量,应用x2检验分析血清ALB、PAB表达量与NSCLC患者临床病理参数的关系;采用Kaplan-Meier分析血清ALB、PAB表达量与NSCLC总生存期(OS)的关系.结果 血清ALB、PAB在NSCLC中的阳性表达量均显著低于良性组患者(P＜0.05).血清ALB、PAB与NSCLC临床分期、淋巴结转移相关(P＜0.05).生存分析显示血清ALB、PAB阳性患者生存期显著高于阴性患者(P＜0.05).多因素COX回归显示NSCLC临床分期、淋巴结转移、血清ALB、PAB是预后的独立影响因素.结论 血清ALB、PAB与NSCLC临床分期以及淋巴结转移有关,血清ALB、PAB阴性表达提示NSCLC患者OS较短,有望作为NSCLC预后预测的分子标志物.

目的：探讨骨髓细胞形态学检查在血液系统疾病诊断中的作用。方法对1164例患者的骨髓细胞形态学结果进行分析。结果1164例患者中缺铁性贫血（IDA）占20.44%，血小板减少性紫癜（ITP）占8.76%，急性髓细胞白血病（AML）占7.22%。结论骨髓细胞形态学在血液系统疾病的诊断中具有重要意义。

Objective To study the correlation between the Sysmex XE-5000 Automatic Hematology Analyzer and microscopy of white blood cell differential count.Methods A total of 100 blood samples were collected from Jianyang People′s Hospital,Sys-mex XE-5000 Automatic Hematology Analyzer was used to do white blood cell differential count,and the inter-batch precision,be-tween-run precision were calculated,and the correlation of the blood cell differential count detected by Sysmex XE-5000 Automatic Hematology Analyzer and artificial microscopy were analyzed.Results The inter-batch precision and between-run precision of Sys-mex XE-5000 Automatic Hematology Analyzer were within the allowable ranges,and these results had good correlation between SysmexXE-5000 Hematology Analyzer and artificial microscopy,the correlation coefficients of Neutrophils,Lymphocytes,Mono-cytes,Eosinophil were 0.978 2,0.909 5,0.827 0,0.868 6 (P <0.05 ),but the correlation on Basophils was not significant(P >0.05).Conclusion Sysmex XE-5000 Automatic Hematology Analyzer for white blood cell differential is rapid,accurate and had better repeatability,and is suitable for detecting the quantity of samples differential,but it is not able to replace the manual micros-copy completely,when the samples have instrument flag,we will be required to perform artificial microscopy in order to improve the accuracy of white blood cell differential count.

Objective To explore the clinical significance of reticulocyte (RET )-related parameters determina-tion in anemia diagnosis .Methods Reticulocyte percentage (RET% ) ,RET count (RET#) ,percent of high fluores-cent reticulocyte (LFR% ) ,percent of middle fluorescent reticulocyte (MFR% ) ,percent of low fluorescent reticulo-cyte (HFR% ) ,and immature RET fraction (IRF) of patients were determined by Sysmex XE-5000 automated blood analyzer and compared with the control group .Results LFR% of patients suffering from hemolytic anemia decrea-ses ,RET% ,RET # ,HFR% ,MFR% and IRF of patients suffering hemolytic anemia were obviously higher than those of normal control group and the difference is quite significant (P< 0 .01);while RET% ,RET # ,HFR% , MFR% ,LFR% and IRF of patients with aplastic anemia were significantly reduced (P< 0 .01);RET% ,RET # , HFR% ,MFR% ,LFR% and IRF of patients suffering iron-deficiency anemia were slightly higher than those of nor-mal control group ,but there were no significant differences among them .Conclusion RET-related parameters are a-ble to reflect the mature of reticulocyte more accurately ,so it can evaluate the hematopoiesis of the bone marrow and it present an important value in the diagnosis and treatment of different types of anemia .

Objective: To prevent and control the hospital infection of Acinetobacter baumannii(ABA) and to guide the correct diagnosis of diseases and reasonable use of antibiotics. Methods: A retrospective research was held to analyze the ABA infection specimen,which were collected in our hospital during 2013. The specimens culture for the pathogens and the drug susceptibility were adopt through a unified standard,and the result was analyzed by software WHONET 5. 4. Results: ABA infection in ICU was 32. 1%,22. 1% in neurosurgery,15. 7% in respiratory medicine. Sputum and throat swab were the main specimens,accounted for 62. 1% and25. 7% respectively. ABA sensitivity to amikacin,ertapenem,gatifloxacin were 100%,sensitivity to levofloxacin was 85. 71%,to ciprofloxacin was 83. 57%,and to imipenem was 85%. ABA resistance to amoxicillin,cefazolin,cefuroxime sodium, cefotetan were 100%, to aztreonam was up to 96. 43%. Conclusions: Acinetobacter baumannii has higher resistance to cephalosporin antibiotics. In order to control or slow the growth of bacteria resistance and hospital infection,it necessary to strength the management of disinfection and isolation in the ward,strictly implement the aseptic operation,and clinical drug treatment should be based on drug sensitivity results.

随着科学技术日新月异的发展，血液分析技术从以前的三分类逐步转向为五分类，为临床提供多参数的检验项目，大大提高了检验人员的工作效率，同时也提高了血液分析的整体质量。我院新进的XE-5000血液分析仪采用激光流式细胞技术并结合细胞化学荧光染色，提高了细胞分析的灵敏度和准确度，同时还能进行体液标本分析，能及时地为临床及时提供可靠的检验结果。现对该仪器的主要性能指标进行评价，其结果报告如下。

Objective: Discuss the clinical application value of HLA-B27 anti gen test in the early diagnosis of ankylosing spondylitis(AS).Method: Use flow cytometry to conduct HLA-B27 antigen test for 30 physical examination testees, 4 1 suspected AS patients and 52 AS patients.Result: The positive rates of physic al examination control group,suspected AS group and AS group are 0%,12.2% and 90.4% respectively.The HLA-B27 positive rates of AS group and suspected AS g roup are evidently higher than that of physical examination group(P<0.01);while the HLA-B27 positive rate of AS group is evidently higher than that of suspect ed AS group(P<0.01).Conclusion: HLA-B27 antigen is related to AS diseas e.HLAB27 antigen test has important significance for the early diagnosis of suspected AS patients.

目的探讨尿沉渣分析仪检测尿中红细胞假阳性。方法以尿沉渣镜检结果为标准,对尿沉渣分析仪的检验结果进行比较分析。结果尿沉渣分析仪检测红细胞存在一定程度的假阳性(15.7%)。结论尿沉渣分析仪检查红细胞与尿沉渣镜检在一定程度上具有互补性,只有联合应用才能解决两者的不足,使检验结果更加可靠。

目的比较雷贝拉唑与奥美拉唑三联疗法治疗幽门螺杆菌(helicobacter pylori,Hp)阳性消化性溃疡的临床疗效。方法 2009年1-12月将96例Hp阳性的消化性溃疡及慢性胃炎患者,根据治疗方法不同随机分为雷贝拉唑组(52例)和奥美拉唑组(44例)。两组联合的抗菌药均为克拉霉素和阿莫西林,疗程1周。各组在治疗后4～8周分别检测Hp感染情况,并观察溃疡的愈合情况。结果雷贝拉唑组Hp根除率及3d症状缓解率分别为96.2%(50/52)、82.7%(43/52),奥美拉唑组分别为88.6%(39/44)、72.7%(32/44),雷贝拉唑组均明显高于奥美拉唑组(P<0.05)。雷贝拉唑组溃疡愈合率为98.1%(51/52),与奥美拉唑组的95.5%(42/44)比较无统计学意义(P>0.05)。结论雷贝拉唑联合克拉霉素和阿莫西林是根除Hp比较有效的一种治疗方案。

目的60例空腹晨尿探讨尿液干化学法与尿沉渣镜检白细胞的相关性。方法610例空腹晨尿以尿沉渣镜检结果为标准对干化学法的检验结果进行比较。结果尿干化学分析法检测白细胞存在一定程度的假阳性12例(4.53%)和假阴性38例(11.01%)。结论尿干化学法检查白细胞与尿沉渣镜检在一定程度上具有互补性,只有联合应用才能解决二者的不足,使检验结果更加可靠。

目的探讨糖化血红蛋白在糖尿病监测中的临床意义。方法采用免疫比浊法及已糖激酶法分别测定糖化血红蛋白(HbAlc)和空腹血糖(FBG)的含量。结果治疗前FBG和HbAlc均明显高于正常对照组(P<0．01);治疗后的FBG和HbAlc与治疗前比较,下降明显(P<0．01)。结论 HbAlc值可判断近2个月血糖波动情况,是监测糖尿病发生、发展的良好指标。

本文对来我院检测HBV"两对半"的4 380例感染者分少儿组(＜15岁)和成人组(＞16岁)进行了血清模式分析,结果大三阳、小三阳、抗-HBs和其他分别占检测总数的27.53 %、28.75 %、27.67 %和16.05 %;大三阳和抗-HBs阳性率少儿组高于成人组,少儿组抗-HBs阳性率占少儿组的47.83 %,小三阳阳性率成人组高于少儿组,P<0.01,差异均非常显著.说明国民身体素质提高很大.

目的了解血清前白蛋白在肝病中的意义.方法采用免疫浊法对50例健康体检者和130例各种肝病血清前白蛋白的含量进行检测.结果健康对照组PA值为244±57 mg/L,慢性肝炎组PA值为161±54 mg/L;急性肝炎组PA值为137±63 mg/L,重症肝炎组PA值为98±42 mg/L;活动性肝硬化组PA值为72±29 mg/L.与健康对照组比较差异具有统计学意义(P＜0.01).结论血清前白蛋白检测是反映肝脏功能的一项敏感指标,在肝病诊断中具有重要的临床意义.