We analyzed clinical features and predictors of outcome in adult patients with new-onset refractory status epilepticus(NORSE). In this retrospective cohort, we analyzed the data of 72 patients with NORSE followed-up for two or more years. Outcome was assessed by Glasgow Outcome Scale(GOS) at discharge and latest follow-up. Mean age of the population was 35.13 ± 16.42 years and average follow-up was 60.34 ± 18.12 months. Average length of stay in hospital was 19.62 ± 14.68 days and mean ICU stay was14.43 ± 13.89 days. Of 72 patients with NORSE, 37(51.3
Meningiomas are the most prevalent CNS neoplasms in adults and display various cytological and histological features associated with higher morbidity and mortality rates and diagnostic complexity. SIRT1 is the class III histone deacetylase (HDAC) that plays a role in the metabolism, aging, and carcinogenesis of organisms and regulates senescence and apoptosis in cells but its role in meningioma is unclear. This study aims to check the expression patterns and interactions of cortactin and SIRT1 proteins in different grades of meningiomas and control arachnoid tissue. In the experimental groups of meningioma, human tissue samples from the three grades of meningioma (n=28) and control arachnoid tissues (n=12) were employed for assessing the expression of cortactin and SIRT1 proteins via immunoblotting, immunohistochemical staining, and immunofluorescence staining. Results revealed a significant increase in cortactin and SIRT1 expression in high-grade meningiomas compared to arachnoid tissue. Co-expression analysis with ki67 revealed correlations between cortactin/SIRT1 and proliferative activity, particularly in grade II and grade III meningiomas. Coimmunoprecipitation assays confirmed a direct interaction between cortactin and SIRT1, which intensified with tumor severity. Here, we report the in vivo interaction between cortactin and SIRT1, revealed by fluorescence resonance energy transfer (FRET) microscopy. In conclusion, this study emphasizes the significance of cortactin and SIRT1 as biomarkers for diagnosis and prognosis in meningiomas, suggesting their potential as targets for therapy
Vinayan, Kollencheri Puthenveettil; Saini, Arushi Gahlot; Jyotsna, Aruna Setumadhava; Singh, Gagandeep; Aneja, Satinder; Jayalakshmi, Sita; Kanhere, Sujata; Sylaja, PN; Panigrahi, Debasis; Verma, Anoop Kumar Author Information
Background: In the last few decades ischemic stroke in young adults (15-45 years) has been increasingly reported. This study was aimed to evaluate the epidemiological features, risk factors and mechanisms of ischemic stroke in young adults. Methods: From June 2018 to February 2020, patients diagnosed with ischemic stroke in the age group of 15-45 years from a tertiary care referral hospital in Hyderabad, India, were consecutively enrolled to study their risk factors, mechanisms and outcome. Results: Out of 971 patients of ischemic strokes 164 (16.8%) were young adults, among whom 113 (69%) were in the age group 36-45 years. Males constituted 71.3% of cohort. Risk factors were alcohol abuse (39.63%), dyslipidemia (39.02%), hypertension (32.32%), smoking (27.44%), diabetes (25.61%), family history of stroke (23.78%) and past history of stroke (12.20%). As regards subtypes, large artery atherosclerosis constituted 26.22%, lacunar stroke 15.24%, cardio- embolic strokes 15.85%, stroke of other etiology 18.9% and stroke of unknown etiology 23.78%. At the time of discharge, death and dependency in younger adults was 8.53% and 45.12% respectively. Conclusion: About 90% of young adults with ischemic stroke had at least 1 established modifiable vascular risk factor. Large artery atherosclerosis both intra and extra-cranial was the most frequent mechanism. Stroke due to other etiologies unique to young adults constituted about one fifth of all ischemic strokes. Keywords: acute ischemic stroke; stroke in young; cerebrovascular accident; etiologies
