Planta

Sicily, Southern Italy, has important genetic resources for azarole (Crataegus azarolus). The region hosts both spontaneous wild populations and various forms of the genus, some of which belong to well-defined specific or intraspecific taxa, while others are hybrids of unclear origin. Not all wild forms can be considered related to C. azarolus, but those included in the sect. Azaroli and thus C. aronia (= C. azarolus var. aronia), which is widespread in western Sicily, certainly can. All these populations are morphologically distinct but coexist in the same area. This preliminary genetic study aims to establish the genetic relationships between wild populations of Crataegus taxa and azarole (C. azarolus). Furthermore, the research addresses the lack of clear taxonomic information regarding the different phenotypes found in Sicily, including both recognized taxa and those still critically underreported. The study is based on SSR (Simple Sequence Repeat) molecular markers, isolated from other Rosaceae species. The findings indicate that at least two groups affiliated with C. azarolus, yet distinct, are present in Sicilian populations. These results could have significant implications for systematics and taxonomy, as well as for conservation and the valorization of plant biodiversity.

Despite the wide amount of scientific contributions published on alien plant species, their diffusion dynamics, and their interactions with native taxa, it is increasingly difficult to slow down their spreading and their negative impact on habitats. Last recent years, in fact, a sharp rise in the number of new alien plant taxa introduced in Italy and Europe has been recorded. The aim of this work is to investigate most of the Italian territory in order to verify whether this alarming trend is still underway. Specimen collections and/or observations of alien plants have been performed in as many as 12 Italian regions. All the collected specimens are stored in public or private herbaria. Taxa have been identified according to the literature from the countries of origin of the investigated taxa, while the nomenclature followed the current international references. Updates on 106 taxa are reported. In particular, among 117 new records, 89 are first records, 27 are changes to status and there is 1 extinction. Seven new taxa for Italian alien flora are reported, two of which are new to Europe. The administrative regions with the highest number of records are Calabria (48), Sardegna (17) and Sicilia (15). Five of the surveyed taxa, for the first time, have been considered invasive aliens to Italian territory. The unfrequent amount of original results provided by this work, over the simple importance of data itself, proves how floristic investigation, still today, represents one of the most effective tools in broadening the current knowledge about alien taxa and their dynamics.

Anthriscus sylvestris (L.) Hoffm. (Apiaceae), commonly known as wild chervil, has gained scientific interest owing to its diverse phytochemical profile and potential therapeutic applications. The plant, despite being categorized as a noxious weed, is traditionally used in treating various conditions like headaches, dressing wounds, and as a tonic, antitussive, antipyretic, analgesic, and diuretic. Its pharmacological importance stems from containing diverse bioactive lignans, especially aryltetralins and dibenzylbutyrolactones. One of the main compounds of A. sylvestris, deoxypodophyllotoxin, among its wide-ranging effects, including antitumor, antiproliferative, antiplatelet aggregation, antiviral, anti-inflammatory, and insecticidal properties, serves as a pivotal precursor to epipodophyllotoxin, crucial in the semisynthesis of cytostatic agents like etoposide and teniposide. The main starting compound for these anticancer medicines was podophyllotoxin, intensively isolated from Sinopodophyllum hexandrum, now listed as an endangered species due to overexploitation. Since new species are being investigated as potential sources, A. sylvestris emerges as a highly promising candidate owing to its abundant lignan content. This review summarizes the current knowledge on A. sylvestris, investigating its biological and morphological characteristics, and pharmacological properties. Emphasizing the biological activities and structure–activity relationship, this review underscores its therapeutic potential, thus encouraging further exploration and utilization of this valuable plant resource.

Sulla coronaria is indigenous to the Mediterranean region. It is grown as fodder in southern Italy because it contains various secondary metabolites with beneficial activities on animals. Recently, its potential use in cosmeceutical treatments for skin problems was reported. In this scenario, to contribute to a possible cosmeceutical application, we characterized the phytochemical profile of Sulla coronaria flowers' hydroalcoholic extract by HPLC-DAD, Folin-Ciocalteu, Aluminum Chloride methods, DPPH assay, and, for the first time, we evaluated the antioxidant and anti-inflammatory activities on dermal fibroblasts. The phytochemical analysis confirmed the significant content of phenolic compounds (TPC 69.8 ± 0.6 mg GAE/g extract, TFC 15.07 mg CE/g extract) and the remarkable presence of rutin, quercetin, and isorhamnetin derivatives that give to the phytocomplex a good antioxidant activity as highlighted by the DPPH assay (IC50 of 8.04 ± 0.5 µg/mL). Through the reduction in NO• and ROS levels in human dermal fibroblasts, the biological tests demonstrated both the safety of the extract and its ability to counteract the inflammatory state generated by Interleukin-1β exposure. Our findings indicate that the antioxidant activities of the phytocomplex are strictly related to the anti-inflammatory action of the Sulla coronaria flowers extract, confirming that this plant could be a valuable source of bioactive molecules for cosmeceutical and nutraceutical applications.

Se optimizó un método fluorométrico para determinar la concentración de histamina en harinas de pescado, el método incluye etapas de extracción en metanol, purificación con una resina de intercambio iónico, reacción de fluorescencia con o-ftaldialdehido y lectura en fluorómetro. Se observó un comportamiento lineal para concentraciones de histamina que oscilan entre 0,1 y 15 pg/5mL y una excelente selectividad de histamina frente a otras aminas biogénicas. Por otro lado, se evaluó las características de recuperación que presenta la resina, la que se cuantificó utilizando soluciones estándares de aminas biogénicas y muestras de harina de jurel Trachurus symmetricus murphyi, (Nichols, 1920) que fueron eluídas a través de la resina para su cuantificación en el fluorómetro. Se registraron recuperaciones de un 100% para concentraciones inferiores a 350 mg/Kg de histamina, y recuperaciones de 85 a 90% para concentraciones superiores. Posteriormente se analizaron muestras de harina de jurel y los resultados se compararon con los entregados por laboratorios de certificación externos a fin de evaluar la correlación entre los respectivosvalores. Los resultados de correlación no mostraron diferencias significativas (p=0,05), sin embargo los valores obtenidos por el método fluorométrico fueron algo más bajos que los de HPLC, debido posiblemente a una retención de la histamina en la etapa de purificación por la resina. Finalmente el método fluorométrico fue aplicado al estudio del efecto de la temperatura de almacenamiento, sobre la materia prima antes de reducción a harina.