Based on the relevant literature of wetland monitoring technology research from 2003 to 2023.Spe-cifically,the trend of the research,research institutions,journals,keywords,and cited frequency were analyzed.The results show that the publication of wetland monitoring technology is on the rise,and it is predicted that by 2024 the number will reach 49.The largest number of publications is Northeast Institute of Geography and Agricul-tural Ecology,Chinese Academy of Sciences.Then one of the most popular journals is Wetland Science and Man-agement,and the main keywords are"wetland protection"and"wetland ecology".The recent studies are focus on the wetland park.The most frequently cited paper titled as"Progress in Wetland Degradation Research"written by Han Dayong.These all show the"wetland degradation"is still a hot research topic.Furthermore,the main moni-toring method is remote sensing(RS)in the present stage.Our results can provide the theoretical guidance for the wetland monitoring technology research in the future.
牦牛肠吻合手术在临床诊疗中不常见,笔者从事兽医工作十余年,仅遇一例. 牦牛肠出现破裂后若未能及时发现,延误了治疗,常导致牲畜死亡或被迫予以淘汰处理. 近日笔者在野外进行了一例牦牛肠吻合手术,效果颇佳,现将治疗经过报道如下.
Objective To detect the specific antibodies of Yersinia pestis phage in Marmota himalayana serum from Guide plague foci by micromethods,and grasp the epidemic dynamics of the animal plague,so as to provide theoretical basis for formulating plague prevention and control measures in this area.Methods The diagnostic Yersinia pestis phage and wild-type Yersinia pestis phage 476 as antigens,conduct neutralization test with 148 Marmota himalayana serum respectively,which were collected from Guide plague foci.After 24 hours,the serum was dripped onto a double-layer Agar medium which is prepared by EV76 bacteria suspension,and then culture at different temperatures.According to the presence of plaque on the medium,it was to determine whether the serum samples contain specific antibodies of Yersinia pestis phage.Results The results showed that highly similar plaques appeared in the culture plate in 28℃and 37℃temperature by the double agar plate method.Conclusion No specif-ic antibody of Yersinia pestis phage was detected in 148 serum samples of Marmota himalayana from the Guide plague foci.It shows that the epidemic situation of plague was in a weak epidemic or resting state among animals in this foci.
2022年1月,互助县一农户养殖的绵羊发生流产疫情.为查明病因,控制疫情,县动物疫控中心开展了此次调查.调查结果显示,发生流产的多为经产母羊,表现为怀孕后期突然流产或产死胎、弱羔,根据流行病学调查和实验室检测结果,确诊为弓形虫感染引发的流产.
In August 2022,there was an unusual mortality event among wild ducks in Qinghai Province,Chi-na.The nucleic acid was extracted from the collected tissues of the dead wild ducks,and the M gene of avian influ-enza virus(AIV)was first detected by universal RT-PCR and then the H5/H7/H9 specific genes were detected by using subtyping primers.The wild duck tissues were found to contain the nucleic acid of H5 subtype AIV.The complete genome of detected AIV was further sequenced by next generation sequence,and the 8 segments of the AIV were obtained.Analysis using Basic Local Alignment Search Tool revealed that,except for the NS gene,the other segments showed the highest homology with those of the H5N1 subtype.The HA gene cleavage site exhibited the characters of highly pathogenic avian influenza(HPAI)virus.The strain detected from the wild ducks was named A/Wigeon/Qinghai/2022(H5N1).Genetic evolution analysis of the HA gene indicated that A/Wigeon/Qinghai/2022(H5N1)belonged to the 2.3.4.4b subclade including H5N1 and H5N8,which has been circulating in wild birds in recent years,while showing a distant genetic relationship with the strains that were prevalent in Qinghai Province from 2005 to 2015.This study indicates that the genetic diversity of H5 subtype AIV derived from wild birds in Qinghai Lake is abundant,emphasizing the need for continuous monitoring of AIV carried by wild birds in the area.
