专业课的课程思政是当前高校思想政治工作的新理念新模式.为了扎实推进高等教育"立德树人"根本任务,我们对中医药院校的生物化学课程思政进行了探索.本文从学校层面的课程思政理念输入、教师层面的思政育人能力培养和具体的课程思政设计工作三方面探讨将课程思政融入生化课堂的一系列方法和举措,从而实现在培养学生专业技能的同时进行价值引领的教学目标.

目的 探究在中药调剂中应用中药处方分析对临床用药安全性的影响.方法 整理并选取2019年1月～2020年1月间门诊部开具并使用的5000张中药处方为研究对象,将2019年1月～2019年6月间未采取中药处方分析的2400张作为参照组;将2019年7月～2020年1月间实施中药处方分析的2600张作为观察组.对比分析两组中药调剂的差错率及不合理处方比例.结果 应用中药处方分析后,观察组中出现差错的概率和用药不合理现象发生概率比参照组均明显降低(P<0.05).结论 在中药调剂中应用中医处方分析,可有效降低中药调剂差错率,规避用药不合理情况的出现,对促进临床用药安全性的提升有一定价值.

目的:对比中药配方颗粒与传统中药饮片的临床效果.方法:以2018年1月～2019年6月期间,选择类风湿性关节炎患者96例,以交替分组法,分成甲组(传统中药饮片)和乙组(中药配方颗粒),各48例.对比两组应用效果.结果:同甲组相比,乙组治疗总有效率高(x2=4.360,P=0.037).同甲组相比,乙组症状缓解时间、平均住院时间短,平均治疗费用少(P<0.05).同甲组相比,乙组满意度评分高(P<0.05).结论:对于类风湿性关节炎患者,应用中药配方颗粒与传统中药饮片治疗,均可获得较好疗效,患者病情均得到有效控制,安全性高.但与传统中药饮片相比较,中药配方颗粒更易于患者接受.

Our aim was to investigate whether polymorphisms in the interleukin-4 (IL-4) gene are associated with the risk of colorectal cancer (CRC) in a Chinese Han population. Six single-nucleotide polymorphisms (SNPs) in the IL-4 were genotyped by Agena MassARRAY in 248 CRC patients and 463 healthy controls. The association of IL-4 polymorphisms with CRC risk was assessed by genetic models, linkage disequilibrium, and haplotype analyses. The results suggested that the CC genotype of rs2243250 confers a lower risk of CRC in the recessive model [odds ratio (OR) = 0.42, 95% confidence interval (CI): 0.19–0.92, P = 0.020]. Similarly, rs2227284 GG was associated with a reduced risk of CRC in the codominant (OR = 0.18, 95% CI: 0.04–0.82, P = 0.027) and recessive (OR = 0.19, 95% CI: 0.04–0.83, P = 0.008) models adjusted for age. Our findings suggested that rs2243250 and rs2227284 in IL-4 are associated significantly with reduced CRC risk, which may facilitate the identification of CRC patients in Chinese populations.

针对中医药大学公共选修程序设计课程所面临的问题,通过问卷调查分析课程目标定位、选课学生特点,提出以混合教学理论为指导,选择Python语言作为公共选修程序设计课程的零基础入门语言,采用“MOOC+SPOC+翻转课堂”的混合教学模式的教学思路,并进行了教学实践探索和总结.

Activated pancreatic stellate cells (PSCs) play a crucial role in the progression of pancreatic fibrosis. Transforming growth factor-β (TGF-β) is one of the strongest stimulator inducing fibrosis. The mitogen-activated protein kinase (MAPK) proteins (including ERK, JNK and p38 MAPK) are known to contribute to PSC activation and pancreatic fibrosis. Previous studies have identified PSC activation induced by TGF-β1 is related to MAPK pathway, but the respective role of MAPK family members in PSC activation still unclear, and which family member may be the key mediator in mice PSC activation still controversial. In this study, we investigated the influence of different MAPK family member (JNK, ERK, and p38 MAPK) on mice PSC activation using an in vivo and in vitro model. The results showed p-JNK, p-ERK and p-p38 MAPK were all over-expressed in CP group, and p-JNK, p-ERK, and p-p38 MAPK were co-expressed with activated PSC. In vitro, TGF-β1 induced JNK and ERK over-expression in PSCs. In contrast, p38 MAPK expression in PSC showed only a very weak increase. JNK- and ERK-specific inhibitors inhibited FN and α-SMA mRNA expression in PSCs, and a p38 MAPK inhibitor had no effect on PSC activation. These findings indicate that JNK and ERK were directly involved in the PSCs activation induced by TGF-β1 and the development of pancreatic fibrosis. p38 MAPK participate in the progression of CP, but it does not respond to TGF-β1 directly and may not be regarded as the target of TGF-β1 induced PSC activation.

AIM:To explore the role of macrophages in chronic pancreatitis (CP) and the effect of Dachaihu decoction (DCHD) on pancreatic fibrosis in mice.METHODS:KunMing mice were randomly divided into a control group, CP group, and DCHD group. In the CP and DCHD groups, mice were intraperitoneally injected with 20% L-arginine (3 g/kg twice 1 d/wk for 6 wk). Mice in the DCHD group were administered DCHD intragastrically at a dose of 14 g/kg/d 1 wk after CP induction. At 2 wk, 4 wk and 6 wk post-modeling, the morphology of the pancreas was observed using hematoxylin and eosin, and Masson staining. Interleukin-6 (IL-6) serum levels were assayed using an enzyme-linked immunosorbent assay. Double immunofluorescence staining was performed to observe the co-expression of F4/80 and IL-6 in the pancreas. Inflammatory factors including monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) and IL-6 were determined using real time-polymerase chain reaction. Western blot analysis was used to detect fibronectin levels in the pancreas.RESULTS:Compared with the control group, mice with 20% L-arginine-induced CP had obvious macrophage infiltration and a higher level of fibrosis. IL-6 serum concentrations were significantly increased. Double immunofluorescence staining showed that IL-6 and F4/80 were co-expressed in the pancreas. With the administration of DCHD, the infiltration of macrophages and degree of fibrosis in the pancreas were significantly attenuated; IL-6, MCP-1 and MIP-1α mRNA, and fibronectin levels were reduced.CONCLUSION:The dominant role of macrophages in the development of CP was mainly related to IL-6 production. DCHD was effective in ameliorating pancreatic fibrosis by inhibiting macrophage infiltration and inflammatory factor secretion in the pancreas.

生物化学是医学生必修的一门基础医学课程.在生物科技飞速发展的时代,转变传统教学模式,建立创新教育模式,是高校教育改革的必然之路.通过生物化学教学改革和实践,培养创新型人才,重在教学观念更新,贵在教学方法探索.文章在积极思考新时期创新人才培养教学理念的基础上,对中医药院校创新人才的培养方式进行了初步探索.

OBJECTIVE To study the effects of Chai Qin Cheng Qi Decoction(CQCQD)(Traditional Chinese Medicine)on severe acute pancreatitis (SAP)complicated liver damage in rats. METHODS Seventy-two SD rats were randomly divided into three groups(n=24):Sham group, SAP group and CQCQD treatment group. SAP model was induced by retrograde injection with sodium taurocholate. The rats in CQCQD group received treatment with CQCQD. After modeling 1 h, 5 h, 10 h, pancreas and liver histopathological changes were observed. The serum levels of interleukin-6(IL-6), amylase(AMS), alanine aminotransferase(ALT) and aspartate transaminase(AST) were detected. IL-6 and monocyte chemotactic protein 1(MCP-1)mRNA in pancreas and liver were assayed. RESULTS Compared with sham group, the activities of AMS,ALT and AST and the serum level of IL-6 in SAP group were increased significantly. The levels of MCP-1 and IL-6 mRNA in pancreas and liver tissue were increased (P<0.05). Compared with SAP group, the activities of AMS,ALT and AST and the level of IL-6 in CQCQD group were reduced significantly (P<0.05). The pancreas and liver tissue pathological damage alleviated. The levels of IL-6 and MCP-1mRNA in pancreas and liver were decreased significantly(P<0.05). CONCLUSIONS MCP-1 takes part in the progression of SAP complicated liver damage; CQCQD can significantly inhibit the expression of pancreas and liver MCP-1, alleviate pathological damage of pancreas and liver in SAP and play a therapeutic role in SAP complicated liver damage.

OBJECTIVETo investigate the effects of transforming growth factor-β (TGF-β) and Smad in chronic pancreatitis (CP) induced by dibutyltin dichloride (DBTC) combined with ethanol, and the effect for prevention and treatment of pancreatic fibrosis by DaChaiHu Decoction (tradeiional Chinese medicine).METHODSNinety-six healthy male Kunming mice were randomly divided into control group (Con group), chronic pancreatitis (CP group) group, Dachai Hu decoction treatment group (DCHD group) (n=32). Then the mice were treated with DBTC 8 mg/kg by tail vein injection and fed with 10% ethanol replacing the normal drinking water to replicate mouse CP model. After three days of modeling the mice were randomly divided into CP group and DCHD group. The mice in DCHD group were administered intragastrically with DaChaiHu Decoction (1 g/ml, 6 g/kg·d) at the following 8 weeks. After modeling for 1 week, 2 weeks, 4 weeks and 8 weeks, the mice were anesthetized and sacrificed(n=8). The morphology and the degree of fibrosischanges of pancreatic tissue were detected by HE staining. The protein expressions of type I collagen and TGF beta R I, p-Smad 2/3 and Smad 7 in pancreatic tissue were detected by Western blot assay. The expressions of MMP-1/TIMP-1 mRNA inpancreatic tissue were detected by RT-PCR.RESULTSCompared with control group, DBTC combined with ethanol induced CP with increased activity of serum amylase and hyaluronic acid level. While in DCHD group, the activity of amylase and hyaluronic acid level were decreased significantly. Compared with control group, the protein expressions of COLA1, TGF beta R I, p-Smad 2/3 in CP Group were elevated,but the expression of Smad 7 was significantly reduced; In DCHD treatment group,the expression of TGF beta R I, p-Smad 2/3 and COLA1 were reduced, and the protein expression of Smad 7 was increased. In 2w and 4w, the level of MMP-1mRNA continued to decrease, while TIMP-1mRNA expression was increased significantly (P<0.01). At each time point of DCHD group, the expression of MMP-1 were markedly increased and TIMP-1 were reduced, compared with those of the CP group (P<0.05).CONCLUSIONSDaChaiHu Decoction play a role in the prevention and treatment of chronic pancreatitis and the development of fibrosis, the mechanism may related to inhibit the activation of TGF beta/Smad signaling pathway, and regulate the balance between synthesis and degradation of extracellular matrix.

OBJECTIVE:To detect the expression ofsignal transducer and activator of transcription 3 (STAT3) in pancreatic tissue of the mouse model of pancreatitis, and to explore its role in the evolution of acute pancreatitis.METHODS:Forty-eight healthy male balb/c mice were randomly divided into 3 groups (n=16):control group (Con) 0.09% NaCl, intraperitoneal injection; mild acute pancreatitis group (MAP) caerulein, intraperitoneal injection; severe acute pancreatitis group (SAP) caerulein plus lipopolysaccharide(LPS), intraperitoneal injection. The mice were sacrificed after 2 h and 6 h after intraperitoneall injection. Serum was isolated for amylase activity. Pancreatic was isolated and weighedto calculate the pancreatic wet weight ratio. Myeloperoxidase (MPO) activity was measured to assess the degree of inflammatory cell infiltration in lung tissue. Using HE staining, the pathological changes of pancreatic and lung were observed under the light microscope. The expression of phosphorylated STAT3 (p-STAT3) was detected by Western blot.RESULTS:Compared with control group, serum amylase activity, pancreatic wet weight ratio and lung MPO activity were significantlyincreased (P<0.05) in MAP and SAP group at each time point, especially SAP group showed higher levels of MPO activity than that in MAP group (P<0.01). The pathological changes of pancreas and lung were observed after modeling in 2 h. Western blot showed the expression of p-STAT3 could be detected in SAP group, the level increased most significantly after modeling 2 h, and decreased slightly after 6 h. The level of p-STAT3 was low in MAP group and negative in Con group at each time point.CONCLUSIONS:The expression of p-STAT3 in MAP and SAP groups are significantly different from that in control group, which indicates that STAT3 isclosely related in acute pancreatitis. Inhibition of STAT3 activity is a potential target to alleviate acute pancreatitis progression.

AIMTo search for a new chronic pancreatitis model in mice suitable for investigating the pathophysiological processes leading to pancreatic fibrosis.METHODSThe mice were randomly divided into 2 groups (n = 50), control group and model group. The mice in model group were given ethanol (10%) in drinking water after injection of dibutyltin dichloride (DBTC) (8 mg/kg BW) in tail vein. The mice in control group were injected with only solvent into tail vein (60% ethanol, 20% glycerine and 20% normal saline) and drank common water. At days 1, 7, 14, 28, and 56 after application of DBTC or solvent, 10 mice in one group were killed at each time point respectively. Blood was obtained by inferior vena cava puncture. The activity of amylase, concentration of bilirubin and hyaluronic acid in serum were assayed. The pancreas was taken to observe the pancreatic morphology by HE staining, and to characterize the pancreatic fibrosis by Masson staining. The expression of F4/80, CD3 and fibronectin (FN) were assayed by immuno-histochemistry or Immunofluorescence technique. Collagen type I (COL1A1) in pancreas were detected by Western blot. The expression of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA in the pancreas was assessed by real time PCR.RESULTSDBTC induced an acute edematous pancreatitis within 1 d. The dilated acini, scattered acinar cell necrosis, and inflammatory cells were found at day 7. Extensive infiltration with inflammatory cells following deposition of connective tissue was observed at day 14. At day 28, level of pancreatic fibrosis was aggravated. The pancreatic tissue was replaced by an extended interstitial fibrosis at the end of 2 mo. There was significant difference in the level of amylase, bilirubin and hyaluronic acid in serum between control group and model group (P < 0.05). The level of COL1A1 and FN in pancreas increased. The expression of MMP-1 mRNA in pancreas decreased, but TIMP-1 mRNA increased at model group.CONCLUSIONDBTC joint Ethanol drinking can induce chronic pancreatitis in accordance with the pathophysiological modification of human. DBTC joint Ethanol-induced pancreatitis in mice is an effective and handy experimental method. The model is suitable to study the mechanism of pancreatic fibrosis in chronic pancreatitis.

目的 分析腹型肥胖与体内炎性因子水平及代谢综合征(MS)之间的关系,探讨儿童腹型肥胖的科学治疗方法.方法 以该院儿科门诊2014年1月-2015年12月接诊的128例单纯性肥胖儿童为研究对象,根据腰围数据进行划分,设腹型组与非腹型组两组.同时对研究对象的体重、身高、臀围(H)、腰围(W)进行测量.采集各研究对象的空腹血并检测其血糖、血脂、超敏C反应蛋白(Hs-CRP)和内脂素水平.结果 腹型组体质指数(BMI)、W、腰臀比(WHR)均明显高于非腹型组差异有统计学意义(t=4.85、3.82、3..08,均P＜0.05);腹型组MS检出率明显高于非腹型组,差异有统计学意义(x2=12.119,P＜0.05);腹型组Hs-CRP及内脂素水平明显高于非腹型组,差异有统计学意义(t=4.612、4.121,均P＜0.05);两组高血压、高血糖、高甘油三酯(TG)血症、低高密度脂蛋白胆固醇(HDL-C)血症的人数比较差异均有统计学意义(x2=8.82、9.15、14.71、7.41,均P＜0.05).结论 在肥胖儿童中,较之非腹型肥胖儿童,腹型肥胖儿童MS患病几率更高;儿童腹部脂肪分布与体内炎症因子及TG的增高有着密切关系.因此,应对腹型肥胖进行积极防治.

The degree of satisfaction of the curriculum is related not only to the degree of the curriculum but also to the degree of the students' development. With the concept of teaching reform micro system engineering, using the SIMPP analysis of the degree of satisfaction of the curriculum, the relevant factors of the students are studied. The results show that, the learning state, learning objec-tives, and the education level of mother affect the students more easily on the curriculum satisfaction. Research shows, in the present curriculum condition, educators should guide the students to study hard, establish a clear and reasonable learning goal, give the students the introduction and analysis of the curriculum, can effectively improve the students on the curriculum satisfaction, enhance the enthusiasm of the study.

The influence extent of students from teachers is related to the teaching effect , which means whether it can successfully promote the study of students and reach the expected teach-ing goal during the teaching process. To have a good teaching effect, guided by the micro-system en-gineering of teaching reform, we used SIMPP to analyze the related factors of the influence of students extent from teachers during the teaching process in TCM colleges and universities. The result shows that the influence extent from teachers is related to not only teachers themselves but students and their self-condition and family backgrounds as well. Going further in researching these factors and the related behavior patterns of influence extent of students from teachers is helpful to making the teach-ing more effective and more targeted.

Accumulated evidences supported IL-8 play an important role during colorectal carcinogenesis. However, few direct-related evidences are available. In this paper, we found that high level of IL-8 was constitutively present both in familial adenomatous polyposis (FAP) tissue and carcinoma tissue. Using primary epithelial cells from FAP samples, we study IL-8 effect on their growth, migration, and colonies formation. The results showed that IL-8 stimulated cells proliferation, migration, and colonies formation. Furthermore, High level of CEA, CK20, and EGFR was detected after exposure to IL-8 in primary epithelial cells. In conclusion, our findings showed that IL-8 promotes the adenoma–carcinoma transition in primary epithelial cells from FAP. Targeting IL-8 at adenoma stage may prevent or reduce carcinogenesis.

The key points and reform measures were investigated for improving students' ability of resolving theproblems in Chinese pharmacy. The importance and necessity of the reform were analyzed from 6 aspects includingcourse orientation, content design, textbook compilation, teaching methods, teachers' development and assessmentmodes. The paper summed up practical experience, introduced specific measures and provided ideas and methodsfor the construction of statistical course of Chinese pharmacy.

导师陈明教授宗经方、时方之旨,灵活组方,善以合方治疗胸痹,且临证多验.文章以陈明教授治疗胸痹的相关验案为例,介绍其选方用药重视病证针对性的临证经验特点.

灼口综合征作为口腔科常见多发病,亦为临床疑难病之一,中医在对其治疗的过程中取得了可靠疗效.文章通过对其病因、病机及常见证型的总结阐述,结合临证验案,分析导师陈明教授治疗灼口综合征的辨治经验.