The incidence of Gastric cancer (GC) has shown a sharp upward trend, and patients with GC complicated by diabetes exhibit significantly worse clinical outcomes and prognosis compared to those without diabetes. Traditional Chinese medicine has played a crucial role in the treatment of both GC and diabetes. Currently, Banzhilian(Scutellaria barbata D. Don) is utilized in the treatment of GC; however, the specific small-molecule monomers it contains and their mechanisms of action have not yet been fully elucidated. This study aims to explore the mechanism of quercetin, a key component of Banzhilian, through network pharmacology, molecular docking, molecular dynamics (MD) simulation, bioinformatics, and in vitro and in vivo experiments. Initially, core targets and key pathways involved in the treatment of diabetes-associated GC (GC-diabetes) were identified using public databases. Subsequently, molecular docking, MD simulation, and survival analysis were performed. Experimental validation included CCK-8 assays, colony formation assays, apoptosis detection, cell cycle analysis, wound healing assays, Transwell migration assays, Western blotting, and mouse subcutaneous tumor formation experiments to evaluate the effects of quercetin, as an active monomer in Banzhilian, on Gastric cancer cells (HGC-27-HG cells) under high-glucose conditions. In this study, quercetin was identified as the key active component, with AKT1, TP53, JUN, MYC, and CCND1 recognized as the target genes, and the PI3K/AKT signaling pathway as the primary regulatory pathway. The results of the study indicate that the proliferation, migration, and invasion capabilities of HGC-27-HG cells are significantly higher than those of HGC-27 cells. However, quercetin inhibited the growth of HGC-27-HG cells, promoted apoptosis, induced cell cycle arrest at the G0/G1 phase, and reduced the cells’ migration and invasion abilities. Furthermore, it downregulated the expression of target genes and their phosphorylation levels. The experimental findings confirmed that quercetin, as an active monomer in Banzhilian, suppresses the proliferation of HGC-27-HG cells by inhibiting the PI3K/AKT/MYC pathway, promoting apoptosis, blocking cell cycle progression, and inhibiting cell migration and invasion.

Background:Intraperitoneal chemotherapy is an effective way to kill free tumor cells in the abdominal cavity. The safety and efficacy of raltitrexed perfusion during radical surgery for elderly patients with colorectal cancer are still unclear. Methods:In accordance with computer-generated random allocation sequences, 116 elderly patients with colorectal cancer who received radical surgery were randomly grouped into the raltitrexed intraperitoneal perfusion group or the saline intraperitoneal perfusion group from January 2020 to December 2021 in the First Affiliated Hospital of Bengbu Medical University. t tests and χ2 tests were used to analyze the difference between the two groups of the clinical characteristics, pathological features, perioperative parameters, and carcinoembryonic antigen mRNA in the peritoneal lavage fluid. Results:No statistically significant differences in postoperative complications after radical surgery were observed between the two groups. No statistically significant differences in peripheral blood indexes were observed between the two groups before surgery or on the first and third days after surgery. One day after radical surgery, the alanine transaminase (54.33 ± 4.93 vs 51.01 ± 5.56) and aspartate transaminase (49.28 ± 4.30 vs 50.99 ± 3.88) in the peripheral blood were higher in the raltitrexed intraperitoneal perfusion group than in the saline intraperitoneal perfusion group. At the same time, no significant difference was found on the third day after surgery. No significant differences in side effects of chemotherapy were observed between the two groups. The positive rate of carcinoembryonic antigen mRNA in the raltitrexed intraperitoneal perfusion group (8.47%) was significantly lower than that in the saline intraperitoneal perfusion group (22.81%) after surgery. Conclusion:Raltitrexed perfusion during radical surgery is safe and feasible for elderly patients with CRC and can reduce the positive rate of carcinoembryonic antigen mRNA in peritoneal lavage fluid, so it can be explored as a treatment option.

Objective: To investigate the value of machine learning and traditional Cox regression models in predicting postoperative survivorship in patients with adenocarcinoma of the esophagogastric junction (AEG). Methods: This study analyzed clinicopathological data from 203 patients. The Cox proportional risk model and four machine learning models were constructed and internally validated. ROC curves, calibration curves, and clinical decision curves (DCA) were generated. Model performance was assessed using the area under the curve (AUC), while calibration curves determined the fit and clinical significance of the model. Results: The AUC values of the 3-year survival in the validation set for the Cox regression model, extreme gradient boosting, random forest, support vector machine, and multilayer perceptron were 0.870, 0.901, 0.791, 0.832, and 0.725, respectively. The AUC values of 5-year survival in the validation set for each model were 0.915, 0.916, 0.758, 0.905, and 0.737, respectively. The internal validation AUC values for the four machine learning models, extreme gradient boosting, random forest, support vector machine, and multilayer perceptron, were 0.818, 0.772, 0.804, and 0.745, respectively. Conclusion: Compared with Cox regression models, machine learning models do not need to satisfy the assumption of equal proportionality or linear regression models, can include more influencing variables, and have good prediction performance for 3-year and 5-year survival rates of AEG patients, among which, XGBoost models are the most stable and have significantly better prediction performance than other machine learning methods and are practical and reliable.

BACKGROUND:Structural maintenance of chromosome protein 4 (SMC4) is crucial for chromosome assembly and separation, but its role and mechanism in cardia adenocarcinoma (CA) are unknown.METHODS:SMC4 expression levels were initially detected by protein profiling in 20 pairs of CA tumor tissues and adjacent normal tissues. The level of SMC4 expression in CA cells was then evaluated using a western blot analysis. Cell proliferation was evaluated by CCK-8 and clone formation tests. Scratch and transwell tests were used to investigate cell migration as well as invasion, while through the flow cytometry, we examined the cell apoptosis and progression of the cell cycle. The regulatory effects of the epithelial-mesenchymal transition (EMT) and the Wnt/β- catenin pathway were investigated using western blot. A tumorigenesis experiment was used to investigate the influence of SMC4 on tumor development in nude mice.RESULTS:This study showed overexpression of SMC4 in CA tissues and cells. Knockdown of SMC4 can significantly inhibit the proliferation, migration and invasion, stimulate cell apoptosis, induce cell cycle arrest in the G0/G1 phase of CA cells, and inhibit tumor growth in vivo. In addition, down-regulation of SMC4 resulted in decreased expression of Bcl-2, Cyclin D1, CDK4, CDK6, β-catenin, phosphorylated GSK-3β, N-cadherin, and Vimentin, with an increased level of proteins, i.e., Bax, cleaved-caspase3, and E-cadherin. When SMC4 was overexpressed, these effects were reversed.CONCLUSION:SMC4 can facilitate the biological progression of CA, suggesting that SMC4 could be a potential therapeutic target for the disease.

This study delves into the synergy between Chinese female tennis players and the potential of Chansu-Medicated Serum in inhibiting breast cancer cell proliferation through apoptosis and G2 arrest. Chinese female tennis players have garnered international recognition for their achievements on the court and their philanthropic endeavors off it. This research investigates the intersection of their impact and the advancement of breast cancer research. Chansu-Medicated Serum, a traditional Chinese medicine derivative, presents promising mechanisms for inhibiting breast cancer cell proliferation, including apoptosis induction and G2 cell cycle arrest. By exploring this conjunction, we aim to shed light on the possible contributions of these athletes and traditional medicine to the ongoing battle against breast cancer.

Abstract This study investigated the predictive performance of machine learning models for adenocarcinoma of esophagogastric union (AEG), based on 287 AEG patient data collected clinically. After grouping, Cox proportional hazards regression model (Cox-PH) and four machine learning models were constructed and internally validated. The AUC values of 3-year survival rate in validation set of Cox-PH, extreme gradient boosting (XGBoost), Random Forest (RF), support vector machines (SVM), and Multi-layer Perceptron (MLP) were 0.870, 0.901, 0.791, 0.832 and 0.725, respectively. The AUC values of 5-year survival rate in validation set of each model were 0.915, 0.916, 0.758, 0.905 and 0.737. The internal validation AUC values of the four machine learning models, XGBoost, RF, SVM and MLP were 0.818, 0.772, 0.804 and 0.745, respectively. In conclusion, compared with Cox-PH, machine learning models do not need to meet proportional assumption or linear regression model, and can include more influencing variables, which has good predictive performance for the 3-year and 5-year survival rate of AEG patients, among which XGBoost model is the most stable and has obvious superiority in prediction performance than other machine learning methods, practical and reliable.

OBJECTIVE:To compare the performance of machine learning models and traditional Cox regression model in predicting postoperative outcomes of patients with esophagogastric junction adenocarcinoma (AEG).METHODS:This study was conducted among 203 AEG patients with complete clinical and follow-up data, who were treated in our hospital between September, 2015 and October, 2020. The clinicopathological data of the patients were processed for analysis using R language package and divided into training and validation datasets at the ratio of 3:1. The Cox proportional hazards regression model and 4 machine learning models were constructed for analyzing the datasets. ROC curves, calibration curves and clinical decision curves (DCA) were plotted. Internal validation of the machine learning models was performed to assess their predictive efficacy. The predictive performance of each model was evaluated by calculating the area under the curve (AUC), and the model fitting was assessed using the calibration curve.RESULTS:For predicting 3-year survival based on the validation dataset, the AUC was 0.870 for Cox proportional hazard regression model, 0.901 for eXtreme Gradient Boosting (XGBoost), 0.791 for random forest, 0.832 for support vector machine, and 0.725 for multilayer perceptron; For predicting 5-year survival, the AUCs of these models were 0.915, 0.916, 0.758, 0.905, and 0.737, respectively. For internal validation, the AUCs of the 4 machine learning models decreased in the order of XGBoost (0.818), random forest (0.758), support vector machine (0.0.804), and multilayer perceptron (0.745).CONCLUSION:The machine learning models show better predictive efficacy for survival outcomes of patients with AEG than Cox proportional hazard regression model, especially when proportional odds assumption or linear regression models are not applicable. XGBoost models have better performance than the other machine learning models, and the multi-layer perception model may have poor fitting results for a limited data volume.

Micro-nano bubbles (MNBs) usually refer to ultrafine bubbles less than 100 mu m in diameter, exhibiting extraordinary physical and chemical properties compared with those of macro-bubbles, and are attracting increasingly attention in both academia and industry. In the current paper, the full life circle of MNBs was reviewed, from emerging MNBs generation approaches to the advanced characterization techniques, and finally to their recently booming applications. The MNBs generation techniques were classified into two categories: the chemical approaches (e.g., electrolysis and chemical reaction, etc.) and the physical approaches (e.g., cavitation, dispersion of gas, solution mixing, temperature alteration, EHD effect, etc.). As to MNBs characterization, both the static and dynamic characterization techniques were discussed, e.g., optical microscope, high-speed photography, rheology, AFM, DLS, NTA, optical flow cytometry, electron microscope, etc., depending on their operating principles. Merits and demerits of those generation and characterization techniques for MNBs were compared and highlighted, as well as their own characteristics and application scopes. The burgeoning applications of MNBs in functional material preparation were subsequently reviewed in details, including material preparation mechanism, properties and application scenarios. While, the MNBs-based surface cleaning, hydrogen production and carbon neutrality were also emphasized. With the development of basic MNBs theory and the booming scientific and industrial demands, the MNBs-related science and technology will flourish, and MNBs will certainly exhibit broader application prospects in the near future.

目的 探究胃癌细胞中CLDN18-ARHGAP26融合突变基因引起的耐化疗药的作用,并探究人参皂苷在治疗因CLDN18-ARHGAP26融合突变基因表达引起的耐化疗药治疗过程中的抗肿瘤作用.方法 采用免疫磁珠抗体标记胃癌细胞系BGC-823的侧群(SP)细胞和非侧群(NSP)细胞,选出NSP细胞转染过表达CLDN18-ARHGAP26融合突变基因的慢病毒载体.用qPCR检测细胞中CLDN18-ARHGAP26融合突变基因和三磷酸腺苷结合转运蛋白G超家族成员2(ABCG2)mRNA水平的表达.用Western blot检测转染上皮-间质转化(EMT)相关蛋白E-Cadherin、Vimentin的表达.用CCK-8检测转染细胞对化疗药奥沙利铂的敏感性.用CCK-8检测人参皂苷对转染细胞耐药性的影响.人参皂苷处理转染细胞后Western blot检测转染细胞的钙黏蛋白E(E-Cadherin)、波形蛋白(Vimentin)的表达.结果 qPCR检测显示转染过表达CLDN18-ARHGAP26融合突变基因慢病毒载体的NSP细胞中CLDN18-ARHGAP26融合突变基因表达高于未转染组,ABCG2 mRNA表达高于未转染组(P<0.001).Western bolt显示过表达CLDN18-ARHGAP26融合突变基因的NSP细胞中E-Cadherin蛋白表达低于未转染组(P<0.05),Vimentin蛋白表达高于未转染组(P<0.01),转染细胞对奥沙利铂的敏感性低于未转染组(P<0.05).人参皂苷和奥沙利铂同时处理转染细胞,细胞存活率低于单纯奥沙利铂处理(P<0.05).人参皂苷处理转染细胞后E-Cadherin蛋白表达高于未处理组(P<0.01),Vimentin蛋白表达低于未处理组(P<0.05).结论 人参皂苷能逆转胃癌细胞中CLDN18-ARHGAP26融合突变基因表达诱导的细胞EMT转化和奥沙利铂的耐药性.

目的 研究miR-32-5p对胃癌细胞MKN-45增殖、凋亡的作用及机制,以及与含硬化蛋白结构域蛋白1(sclerostin domain-containing protein 1,SOSTDC1)的关系.方法 通过R语言分析筛出相比于正常胃组织,胃癌组织中有2倍以上表达差异的所有miRNA,同时预测差异2倍以上miRNA的靶基因,在其中确定研究目标miR-32-5p及SOSTDC1.采用qRT-PCR研究胃上皮细胞(GES-1)及胃癌细胞(MGC-803、BGC-823、SGC-7901、MKN-45)中miR-32-5p的表达水平.对MKN-45细胞进行转染,使其分为miR-NC mimics组(Lipofectamine 2000与miR-NC mimics共同转染)、miR-32-5 p mimics组(Lipofectamine 2000与miR-32-5 p mimics共同转染)、miR-NC inhibitor组(Lipofectamine 2000与miR-NC inhibitor共同转染)、miR-32-5 p inhibitor组(Lipofectamine 2000与miR-32-5 p inhibitor共同转染).通过qRT-PCR检测过表达和敲除转染效率.通过集落克隆实验及CCK-8实验检测细胞增殖.采用流式细胞术检测细胞凋亡.通过Western blot实验检测miR-32-5 p对凋亡相关蛋白(Bcl-2、Bax、cleaved-Caspase-3)、Wnt/β-catenin通路相关蛋白(β-catenin、p-GSK3β)的影响.利用基因表达谱交互分析(gene expression profiling interactive analysis,GEPIA)网站分析胃癌组织中SOSTDC1的表达情况.TargetScan则用于分析miR-32-5p与SOSTDC1之间的调控关系.通过Western blot实验检测miR-32-5p对SOSTDC1蛋白的影响.结果 miR-32-5p在胃癌组织中高表达,与正常胃组织相比具有2倍以上表达差异(P<0.05),且miR-32-5p与SOSTDC1呈靶向关系.与胃上皮细胞GES-1相比,miR-32-5p在胃癌细胞系MGC-803、BGC-823、SGC-7901、MKN-45中显著高表达(P<0.05).miR-32-5p mimics组细胞中miR-32-5p的表达相比于miR-NC mimics组增加(P<0.05);miR-32-5p inhibitor组细胞内miR-32-5p的表达水平则较miR-NC inhibitor组下降(P<0.05).miR-32-5p mimics组细胞增殖能力与miR-NC mimics组相比增强(P<0.05);miR-32-5p inhibitor组细胞增殖能力则较miR-NC inhibitor组下降(P<0.05).miR-32-5p mimics组细胞凋亡率较miR-NC mimics组下降(P<0.05);miR-32-5p inhibitor组细胞凋亡率则较miR-NC inhibitor组升高(P<0.05).与miR-NC mimics组相比,miR-32-5p mimics组Bcl-2、β-catenin、p-GSK3β的蛋白表达水平升高,而Bax、cleaved-Caspase-3的蛋白水平下降(P<0.05);与miR-NC inhibitor组相比,miR-32-5p inhibitor组Bcl-2、β-catenin、p-GSK3β的蛋白表达下降,而Bax、cleaved-Caspase-3的蛋白表达上升(P<0.05).与正常胃组织相比,SOSTDC1在胃癌组织中低表达(P<0.05).miR-32-5p与其下游靶基因SOSTDC1相互结合.与miR-NC mimics组相比,miR-32-5p mimics组SOSTDC1的表达下降(P<0.05);而与miR-NC inhibitor组相比,miR-32-5p inhibitor组SOSTDC1表达增加(P<0.05).结论 miR-32-5p在胃癌细胞中通过抑制SOSTDC1的表达介导Wnt/β-catenin通路的激活而促进细胞增殖、抑制细胞凋亡.

Background:Gastric cancer (GC) is a highly prevalent tumor type. The dysregulated expression of melanoma deficiency factor 2 (AIM2) has been observed in a range of tumor types. Herein, we explore the role of AIM2 in the regulation of GC progression.Methods:Gastric cancer cells BGC-823 and MGC-803 in logarithmic growth phase were divided into blank group (control), Control group (NC) and SH-AIM2 group, respectively. Control group and SH-AIM2 group were transfected with AIM2 NC and SH-AIM2, respectively. Nude mice were divided into blank group (control) and SH-AIM2 group, and the treatment methods were the same as above. Differential AIM2 expression in GC tissues was assessed via bioinformatics analyses, after which western blotting was used for analyzing the AIM2 levels in tumor and paracancerous tissues from five stomach cancer patients. In addition, qPCR and protein imprinting were used to assess AIM2 expression levels in GC cells, and AIM2 knockdown was conducted in MGC-803 and BGC-823cells, after which colony formation and EdU incorporation assay were utilized to assess cell proliferation. The oncogenic role of AIM2 was then assessed in mice and validated through immunohistochemical analyses.Results:GC tissues and cell lines exhibited marked AIM2 overexpression. AIM2 knockdown significantly impaired GC cell proliferation and migration, as confirmed through in vitro assays. In vivo experiments showed that both the increment ability and invasion and migration ability of AIM2 knockdown group were significantly lower than that of control and NC the change of AIM2 protein level would affect the change of MAPK pathway related protein level.Conclusions:AIM2 knockdown markedly suppresses the proliferation, migration, as well as invasion of GC cells via the inhibition of MAPK signaling, thereby slowing tumor progression. Overall, these results suggest that further analyses of AIM2 may offer clinically valuable insights that can aid in the treatment of human GC.

Background. Nonstructural maintenance of non-SMC condensin I complex subunit G (NCAPG) exerts critical effects on cancer progression. However, its biological roles in tumorigenesis and metastasis remain unclear. Thus, we aimed to assess the prognostic utility of NCAPG in stomach adenocarcinoma (STAD) and its potential as a tumor biomarker. Methods. Pan-cancer expression profile dataset from public databases and corresponding clinical information were extracted. Single-sample gene set enrichment analysis (ssGSEA) was performed for the evaluation of immune correlations pan-cancer. Subsequently, we focused on STAD and evaluated the methylation profiles, copy number variants (CNVs), and single nucleotide variants (SNVs). Immune features were analyzed between high and low NCAPG expression groups. Differential analysis was performed between high and low expression groups to identify differentially expressed genes (DEGs). Prognostic DEGs were screened by univariate analysis, and an NCAPG-based risk model was constructed based on the prognostic DEGs and LASSO analysis. Results. NCAPG expression in STAD was significantly and positively correlated with four immune checkpoints, namely, CTLA4, PDCD1, LAG3, and CD276, but was negatively correlated with the infiltration of most immune cells. High and low NCAPG expression groups had differential overall survival, tumor mutation burden, and differential enrichment of therapeutic-related pathways. An immune risk scoring model related to NCAPG expression and immune score was constructed which showed a favorable performance in predicting STAD prognosis as well as predicting the response to immunotherapy. In addition, we found a higher mRNA stemness index (mRNAsi) in the high-risk group and a positive correlation between NCAPG expression and mRNAsi. Conclusion. NCAPG was suggested to be involved in the regulation of tumor microenvironment in STAD. High NCAPG expression was related to high tumor stemness and good prognosis. The immune risk model had a potential to predict STAD prognosis and help directing therapeutic treatment.

In this study, we aimed to compare clinical efficacy of laparoscopic surgery and open surgery for colorectal cancer in the Huaihe River Basin in China. A total of 92 patients with colorectal cancer were selected as subjects. Patients were divided into control group (open surgery, n = 27 cases) and test group (laparoscopic surgery, n = 28 cases). Our results showed that the duration of operation (122.54 +/- 14.85) min and length of incision (4.51 +/- 1.065) cm were shorter in the experimental group than those in the control group. The intraoperative blood loss of the experimental group was (161.12 +/- 10.694) ml, which was less than that of the control group (218.53 +/- 15.369) ml (P < 0.05). Patients in the experimental group have higher Five-year survival rate and lower incidence of postoperative complications than those in the control group (all P < 0.05). In conclusion, in the treatment of colorectal cancer, laparoscopic surgery has a better clinical effect than open surgery, which is worthy of clinical application.

目的 探讨课程思政在肿瘤外科教学中的合理比重.方法 选取蚌埠医学院临床医学本科专业学生220名,随机分为10 min组(试验组,n=116)和5 min组(对照组,n=104).试验组在在每节课融入10 min课程思政内容,对照组在每节课融入5 min课程思政内容.课后对学生进行章节理论考核、课堂满意度调查问卷和人文关怀能力量表调查.结果 两组章节理论考核成绩无显著差异(P>0.05);试验组课堂满意度明显优于对照组(P<0.05);试验组的人文关怀能力量表得分明显高于对照组(P<0.01).结论 肿瘤外科教学中设置合理的课程思政的教学比重可以获得更好的教学效果.

Soy isoflavones are natural tyrosine kinase inhibitors closely associated with decreased morbidity and mortality of various tumors. The activation of tyrosine kinases such as ERBB2 is the mechanism by which cholecystitis transforms into gallbladder cancer (GBC), therefore, it is important to investigate the relationship between long-term exposure to soy isoflavones and the occurrence and progression of GBC. This case-control study (n = 85 pairs) found that the high level of plasma soy isoflavone-genistein (GEN) was associated with a lower risk of gallbladder cancer (>326.00 ng/mL compared to <19.30 ng/mL, crude odds ratio 0.15, 95% CI 0.04-0.59; P for trend = 0.016), and that the level of GEN exposure negatively correlated with Ki67 expression in GBC tissue (n = 85). Consistent with these results, the proliferation of GBC cells was inhibited in the long-term exposure models of GEN in vitro and in vivo. The long-term exposure to GEN reduced the tyrosine kinase activity of ERBB2 and impaired the function of the PTK6-AKT-GSK3b axis, leading to downregulation of the MCM complex in GBC cells. In summary, long-term exposure to GEN associated with soy products intake might play a certain role in preventing GBC and even inhibiting the proliferation of GBC cells. (c) 2022 Science China Press. Published by Elsevier B.V. and Science China Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

目的 研究lncRNA-AC005062.1对结直肠癌细胞侵袭及迁移的影响并研究其机制.方法 实时荧光定量PCR(qRT-PCR)检测新鲜结直肠癌及癌旁正常组织中lncRNA-AC005062.1表达差异,通过siRNA转染结直肠癌细胞HT29,敲低lncRNA-AC005062.1表达量,qRT-PCR验证转染效率.其次,通过划痕实验检测HT29细胞迁移变化;Transwell实验检测HT29细胞侵袭变化;Western blot检测上皮间质转化(EMT)相关分子(E-Cadherin、N-Cadherin、Vimentin)的表达量改变.结果 与癌旁正常组织相比,结直肠癌组织中lncRNA-AC005062.1表达量明显增高(P<0.05),敲低HT29细胞lncRNA-AC005062.1表达量后,细胞侵袭及迁移能力明显降低(P<0.05),细胞中的E-Cadherin蛋白表达量明显增高(P<0.05),而N-Cadherin及Vimentin表达量明显降低(P<0.05).结论 lncRNA-AC005062.1在结直肠癌中表达明显升高,并且影响HT29细胞侵袭及迁移能力,这种改变可能与E-Cadherin、N-Cadherin、Vimentin的表达及逆转EMT进程有一定相关性.

Certain progress has been made in the therapeutic method against gastric cancer such as surgical operation combined with chemotherapy and radiation therapy in recent years. But the therapeutic efficacy and prognosis on gastric cancer was still not satisfactory. The function of exosome of miR-328–3p secreted by bone marrow stromal cells (BMSCs) on restraining the gastric cancer was studied in the present study. The BMSCs with highly-expressed miR-328-3p was established. The exosome in cell supernatant was collected. The exosome of BMSCs and MSCs with highlyexpressed miR-328-3p was added into SGC-7901 cells followed by analysis of miR-328-3p level by Real-time PCR and TFF3 (Trefoil Factor 3) level in exosome by Western blot, cell proliferation, expression of E-cadherin, Vimentin and Caspase-3. miR-328-39 expression was reduced and TFF3 was elevated in gastric cancer tissue ( P < 0.05). miR-328-3p was upregulated and TFF3 was downregulated after addition of BMSCs exosomes along with increased cell proliferation and reduced E-cadherin and Caspase3 expression ( P < 0.05). In conclusion, exosome of BMSCs could be regulated by miR-328-3p and TFF3 expression is restrained so as to regulate the biological behaviors of gastric cancer cell.

Gastric cancer is one the most common human malignancies, with an increased incidence year by year. The underlying mechanisms of Claudin 18 (CLDN18) was involvement in patients with gastric cancer remain poorly understood. We therefore investigated the function of CLDN18 in patients with gastric cancer. Blood samples were collected from gastric cancer patients. CLDN18 and CLDN18 expression were measured using Microarray and qPCR. MIT assay, Transwell assay cells, LDH activity and Caspase-3/9 activity and Flow cytometry were used to measure the effects of ARHGAP26 and CLDN18 on cell growth in gastric cancer. We firstly found that CLDN18 expression were increased in patients with gastric cancer. Then, up-regulation of CLDN18 promoted cell growth in gastric cancer. Down-regulation of CLDN18 induced apoptosis in gastric cancer. Cancer-promoting genetic of CLDN18 is compromised by CLDN18-ARHGAP26 in gastric cancer cell. Down-regulation of ARHGAP26 rescues the effects of CLDN18-mediated tumor promotion effects on gastric cancer cell. CLDN18-ARHGAP26 mediated tumor suppressive effects on gastric cancer cells by ABCG2 and ABCB1 pathway. These results provide evidence that serum CLDN18-ARHGAP26 as a biomarker for tumor promoting genetic in gastric cancer via ABCG2 and ABCB1 pathway.

Objective:To explore the risk factors for recurrence and metastasis of breast cancer after modified radical mastectomy.Methods:The clinical data of 155 breast cancer patients underwent modified radical mastectomy in three hospitals from March 2014 to September 2015 were analyzed retrospectively According to the follow-up results, there were 49 patients with recurrence and metastasis, while 106 patients with no recurrence and metastasis. Statistical analysis were performed by using SPSS22.0 software.The univariate analysis of postoperative recurrence and metastasis was performed by using χ2 test, while Logistic regression model was used for multivariate analysis. A P value of<0.05 was considered as statistically significant difference.Results:The rate of tumor recurrence and metastasis was 31.6% (49/155) among the 155 patients underwent modified radical mastectomy, including 11 patients with local recurrence and 38 patients with distant metastasis. The factors in univariate analysis that were significantly associated with postoperative recurrence and metastasis were analyzed by Logistic regression. In terms of vascular cancer thrombus, primary tumor diameter of ≥5 cm, axillary lymph nodes metastasis of ≥4, TNM staging for Ⅲ, the histologic grading Ⅲ and negative hormone receptor (ER, PR) were considered as independent risk factors for recurrence and metastasis of breast cancer patients after modified radical mastectomy(P<0.05), while anthracycline combined with plethycline chemotherapy, postoperative adjuvant chemoradiotherapy and endocrine therapy were protective factors for recurrence and metastasis of breast cancer (P<0.05).Conclusion:Independent risk factors for breast cancer recurrence and metastasis after modified radical mastectomy including vascular cancer thrombus, the diameter of primary tumor, the number of axillary lymph node metastasis, TNM staging, histological grade and hormone receptor status. A comprehensive prevention and therapeutic treatment based on the above factors might reduce the risk of recurrence and metastasis and could improve the patients’ prognosis.