介绍了烟草花叶病综合立体生物防治的意义及原则;建立了"以农业防治为基础,生物防治为重点,物理防治为辅助,化学防治为补充"的综合立体生物防治技术.

Considering the tobacco mosaic virus (TMV)and brown spot disease prevailing in Lincang new tobacco-growing area,organic induced resistant agent (DMP),Bacillus subtilis and Trichoderma harzianum were studied comparatively on flue-cured tobacco resist-ance.Three kinds of selected organic induced resistant agent (DMP),Bacillus subtilis and Trichoderma harzianum are used in flue-cured tobacco fields to observe the control effect.The result shows that four methods all have significant effect on the tobacco mosaic virus (TMV)and brown spot disease.Among which DMP +Trichoderma harzianum +Bacillus subtilsi performed best on TMV and brown spot disease with a control effect of 55.1 2% comparing with blank control effect for brown spot disease and 92.35% for (TMV), with a control effect of 4.7% comparing with chemical control effect for brown spot disease and 1 3.0% for (TMV).

An application research of dry mycelium of Penicillium chrysogenum (DMP) cooperate with two kinds of bio-control bacterium was carried out in Lincang Tobacco-growing area. The results indicated that,employ DM P in the plant hole before tobacco seedling transplant,then spray trichoderma agent in re-settling stage and before topping,and root-irrigate bacillus agent in the period of vigorous grow th can sig-nificantly improve the length,the width and the area the biggest waist leaf in squaring period and mature period compare with conventional control,blank control and deal with DMP only. The relative control effect of the above mentioned treatment to tobacco mosaic virus amounted to 80.12%,higher than 25.30%of conventional control,higher than 25.90% of treatment deal with DMP. The relative control effect of the above mentioned treatment to tobacco blown spot amounted to 62.24%,higher than 5.83% of the con-ventional control,higher than 13.29% of the treatment deal with DMP only. The relative control effect of the above mentioned treatment to tobacco black shank amounted to 77.42%,15.32 points higher than the conventional control,higher than 26.61% of the treatment deal with DMP. The yield of the above men-tioned treatment is 1886.70kg/hm2,196.80kg/hm2 higher than the blank control,96.60kg/hm2 higher than conventional control. The production value of tobacco leaf reached 49450.41 Yuan per hectare, 6493.15 Yuan per hectare higher than the blank control,3104.72 Yuan per hectare higher than conven-tional control.

[目的]明确哈茨木霉菌、萎缩芽孢杆菌2种微生物制剂与有机诱导抗病剂在烤烟生产上的组合应用效果.[方法]通过苗期试验和大田试验研究哈茨木霉菌、萎缩芽孢杆菌制剂分别与有机诱导抗病剂(DMP)组合应用对烤烟农艺性状及病害防治的影响.[结果]苗期试验结果表明:DMP分别与哈茨木霉菌、萎缩芽孢杆菌组合应用对烟苗主要农艺性状的提高作用优于DMP、哈茨木霉菌和萎缩芽孢杆菌单独施用,DMP与萎缩芽孢杆菌组合应用对烟苗株高、叶片数、茎直径、地上部分鲜重、地下部分鲜重、地上部分干重和地下部分干重的提高作用最好.大田试验结果表明:在移栽时再施用等量DMP,苗期采用DMP与哈茨木霉菌、萎缩芽孢杆菌组合育苗的烟株大田期主要农艺性状和对烟草黑胫病和花叶病的相对防效均高于单独采用DMP、哈茨木霉菌和萎缩芽孢杆菌育苗的处理,其中以DMP+萎缩芽孢杆菌组合育苗的处理对烟草黑胫病、烟草花叶病的防控效果最好,与常规防治相比,相对防效分别为64.18％和71.43％.[结论] DMP+萎缩芽孢杆菌组合在提高烤烟农艺性状及防治烟草黑胫病和花叶病病害方面效果显著,值得进一步开发应用于烤烟生产.

通过田间小区试验,对青霉菌灭活菌丝体(DMP)防控水稻病害及增产作用进行探索.结果表明,DMP用于水稻育苗能够壮苗促生长,明显提高秧苗株高、鲜质量和干质量等农艺性状,且其改善作用在使用合适的剂量时更明显.DMP处理的水稻秧苗大田移栽后可提高每丛的有效穗数、成穗率、穗实率,并提高千粒质量,降低秕粒比率.同时DMP对稻曲病的相对防效为56.28％ ～79.87％,对纹枯病的相对防效为36.10％～69.72％.这可能是DMP诱导植物产生系统获得性抗病性的能力在水稻上发挥出较好的效果.此外,还能够提高水稻产量,且产量的提高幅度随DMP用量的增加而增高.