Objective To observe the effects of laurocapram and borneol as transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids, hormone-sensitive lipase (HSL) and hydroxymethylglutaryl CoA (HMG-CoA) reductase in hyperlipidemia rabbits. Methods Forty New-Zealand rabbits were randomly divided into 5 groups using the random number table method, with 8 rats in each group. Rabbits in the blank group were fed routinely with a normal diet; rabbits in the other groups were fed with high-fat diet for 12 weeks to establish the hyperlipidemia model. Rabbits in the blank and the model groups were not given any intervention. After the model was prepared successfully, rabbits in the non-transdermal penetration enhancer group received herbal cake-partitioned moxibustion without transdermal penetration enhancers; rabbits in the laurocapram group and the borneol group received herbal cake-partitioned moxibustion with laurocapram or borneol respectively. After 4 weeks of treatment, the serum was isolated and enzyme-linked immunosorbent assay (ELISA) was applied for the detection of HSL and HMG-CoA reductase. The liver tissues were isolated, and total cholesterol (TC) and triglycerides (TG) were measured by enzymatic methods. One-step method was applied for high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) detection, and transmission turbidimetry was for apolipoprotein A1 (Apo-A1) and apolipoprotein B (Apo-B) detection. Results The serum concentrations of the drugs in the laurocapram and the borneol groups were significantly higher than those in the non-transdermal penetration enhancer group (both P <0.05); all drug penetrations in the borneol group were significantly higher than those in the laurocapram group (both P <0.05), except for tanshinone IIA. Compared with the non-transdermal penetration enhancer group, the HSL was significantly increased while the HMG-CoA reductase was significantly decreased in the laurocapram and the borneol groups (both P <0.05); between groups, the HSL in the borneol group was significantly higher than that in the laurocapram group ( P <0.05). Compared with the blank group, the levels of LDL-C, TG, TC and Apo-B in rabbit liver were significantly increased in the model group ( P <0.05); compared with the model group, the levels of LDL-C, TG, TC and Apo-B in the non-transdermal penetration enhancer, the laurocapram, and the borneol groups were significantly decreased (all P <0.05); between groups, the TG and TC in the laurocapram group and the LDL-C, TG, TC and Apo-B in the borneol group were significantly lower than those in the non-transdermal penetration enhancer group (all P <0.05), and the TG, LDL-C and Apo-B in the borneol group were significantly lower than those in the laurocapram group (all P <0.05). Compared with the blank group, the HDL-C and Apo-A1 were significantly decreased in the model group (both P <0.05), while compared with the model group, the HDL-C and Apo-A1 were significantly increased in the non-transdermal penetration enhancer, the laurocapram, and the borneol groups (all P <0.05). Between groups, the Apo-A1 in the laurocapram group, the HDL-C and Apo-A1 in the borneol group were significantly higher than those in the non-transdermal penetration enhancer group (all P <0.05). Conclusion The application of laurocapram and borneol, as transdermal penetration enhancers, in herbal cake-partitioned moxibustion can promote the penetration of the drugs in the herbal cake, increase the levels of HDL-C and Apo-A1, improve the metabolism of HSL and HMG-CoA reductase, and also simultaneously reduce the levels of TC, TG, LDL-C and Apo-B in the liver. The transdermal penetration enhancement effect of borneol is slightly better than or equivalent to that of laurocapram.

OBJECTIVE:To observe the effect of moxibustion on respiratory function and contents of histamine and neuropeptides in skin tissue of "Feishu" (BL13) in asthmatic rats, so as to explore its mechanisms underlying improvement of asthma. METHODS:Thirty-six SD rats were randomly divided into normal control, model and moxibustion groups, with 12 rats in each group. The asthma model was established by subcutaneous injection (at the back and groin, 0.5 mL) and i．p. injection (1 mL)of mixture solution of ovalbumin (OVA), Aluminium Hydroxide gel and 0.9% sodium chloride solution, and repeated nasal drip of 1% OVA. Moxibustion was applied to bilateral BL13 for 15 min, once daily for 14 days. The inspiratory and expiratory resistance, and pulmonary ventilation compliance were detected by using a small animal pulmonary function tester under anesthesia. The contents of histamine, vasoactive intestinal peptide (VIP), substance P (SP) and calcitonin gene-related peptide (CGRP) in the local skin tissue of the left BL13 were assayed by using enzyme-linked immunosorbent assay (ELISA). RESULTS:After modeling, the inspiratory and expiratory resistance were significantly increased (P<0.01), and the lung ventilation compliance was significantly decreased in the model group relevant to the normal control group (P<0.01). Outcomes of ELISA showed that the content of VIP was significantly lower (P<0.01), and those of histamine, SP and CGRP in the skin of left BL13 were significantly increased in the model group relevant to the normal control group (P<0.01). Following the intervention, the inspiratory and expiratory resis-tance, cutaneous histamine, SP and CGRP contents were significantly decreased (P<0.01), while the lung ventilation compliance and cutaneous VIP level were significantly increased in the moxibustion group in contrast to the model group (P<0.01, P<0.05). CONCLUSION:Moxibustion of BL13 can improve pulmonary function in asthma rats, which may be related to changes of levels of histamine and neuropeptides as VIP, SP and CGRP in the local skin tissues of BL13.

目的:观察隔药饼灸对慢性支气管炎模型大鼠外周血中T细胞亚群含量的影响,探讨隔药饼灸防治慢性支气管炎的可能机制.方法:48只SD大鼠随机分为空白组、模型组、隔药饼灸组、核酪口服液组4组各12只,采用改良烟熏法复制慢性支气管炎大鼠模型,流式细胞术检测血清中T细胞亚群含量变化.结果:与空白组比较,模型组大鼠外周血中CD4+、CD8+、CD4+/CD8+明显降低;与模型组比较,隔药饼灸组、核酪口服液组CD4+、CD8+及CD4*/CD8+升高;与核路口服液组比较,隔药饼灸组CD4*、CD8+、CD4+/CD8+升高更明显.结论:隔药饼灸可能通过上调外周血中CD4+、CD8+T细胞亚群含量,提高CD4+/CD8+,起到对慢性支气管炎的防治作用.

为了适应高校大学生的学习,本研究团队对《实验针灸学》移动交互式数字教材进行开发,以适应大学生所处的互联网时代,促进高校教学改革.文章对移动交互式数字教材在《实验针灸学》中的教学与应用进行探索,供广大教育工作者参考.

目的:运用Meta分析的方法评价温针灸治疗膝关节骨性关节炎的临床疗效.方法:计算机检索Cochrane Library、PubMed、Web of Science、中国知网(CNKI)、维普全文数据库(VIP)、万方数据库(WF)中温针灸治疗膝关节骨性关节炎的临床随机对照试验文献,检索时间限定为建库至2017年10月,采用专人筛选文献和提取数据,采用RevMan 5.3进行Meta分析.结果:共纳入10篇文献,819例患者,Meta分析结果显示,温针灸治疗组与对照组比较,总有效率[OR =5.22,95％ CI(3.45,7.89),P＜0.00001],治愈率[OR =2.32,95％ CI(1.69,3.19),P＜0.00001],具有明显统计学意义.结论:温针灸治疗膝关节骨性关节炎的疗效肯定,但由于纳入的文献方法学质量不高,需要高级别的临床临床随机对照试验加以验证.

目的:观察隔药饼灸对慢性支气管炎大鼠p-p38MAPK、ICAM-1的影响.方法:将48只SD大鼠随机分成空白组、模型组、隔药饼灸组、隔纸垫灸组,每组12只.选用改良烟熏法建立大鼠慢性支气管炎模型,干预后,测定肺泡灌洗液中性粒细胞含量,各组大鼠ICAM-1 mRNA选用RT-PCR术测定、ICAM-1及p-p38MAPK蛋白选用Western blot检测.结果:与空白组比较,模型组中性粒细胞、p-p38MAPK、ICAM-1 mRNA及蛋白含量增高显著(P<0.01).与模型组比较,隔药饼灸组中性粒细胞数目减少(P<0.05),p-p38MAPK、ICAM-1 mRNA及蛋白含量显著降低(P<0.01).与隔纸垫灸组比较,隔药饼灸组中性粒细胞、p-p38MAPK蛋白、ICAM-1 mRNA及蛋白降低更明显(P<0.05).结论:隔药饼灸可通过减少p-p38MAPK蛋白含量、降低ICAM-1 mRNA及其蛋白水平,抑制中性粒细胞的释放,以改善炎性反应环境治疗慢性支气管炎.

目的 研究电针治疗早期对帕金森病(Parkinson's disease,PD)小鼠模型步态运动行为的影响.方法 步态运动行为训练合格的C57BL/6小鼠40只,随机分为对照组、模型组、美多芭组和电针组,每组10只.对照组腹腔注射生理盐水,模型组、美多芭组和电针组腹腔注射MPTP诱导亚急性PD小鼠模型.对照组、模型组不予干预,美多芭组给予美多芭灌胃,电针组给予电针"合谷""太冲"干预,连续7d后进行步态行为学实验、爬杆实验、悬挂实验、转棒实验.结果 模型组与对照组比较,平均体转角、单支撑时相、双支撑时相、足迹平均强度、对侧协调性、速度、平均步行周期、支撑时相、瞬时速度等步态指标均有明显变化(P<0.05,P<0.01);与模型组比较,电针组、美多芭组的单支撑时相_左后、单支撑时相_右后、速度、平均步行周期、瞬时速度_左后、瞬时速度_右后、左前推进指数、右前推进指数等步态指标有明显改善(P<0.05,P<0.01);模型组与对照组比较,下半部时间、总时间等爬杆指标均明显增加(P<0.01);模型组与对照组比较,掉落潜伏期、在杆足数得分等悬挂指标均有明显下降(P<0.05,P<0.01);模型组与对照组比较,转棒指标掉落潜伏期明显下降(P<0.05);电针组、美多芭组与模型组比较,爬杆、悬挂、转棒指标均无明显变化(P>0.05).结论 电针在治疗早期即可改善PD小鼠的步态运动行为,全自动小鼠步态分析系统能够在电针治疗早期准确敏感反映PD小鼠步态运动行为的细微改变.

目的:氮酮和冰片作为促透剂运用于药饼中对高脂血症兔进行隔药饼施灸,观察其对高脂血症兔血脂及炎性反应因子的调节作用.方法:将40只新西兰兔随机分为正常组、高脂模型组、无促透剂组、氮酮组和冰片组,每组8只.正常组普通饲料喂养,其余各组用高脂饲料喂养12周成高脂模型,成模后采用不同促透剂运用于隔药饼灸施灸4周后,检测兔血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及TNF-α、INF-γ的含量.结果:3个隔药饼灸组均显著降低高脂模型兔血清TC、TG、LDL-C的含量(P<0.05)、显著升高HDL-C的含量(P<0.05);氮酮组降低TC和TG、冰片组降低TC、TG、LDL-C的效果优于无促透剂组(P<0.05);冰片组降低TC、TG、LDL-C的效果优于氮酮组(P<0.05);3个隔药饼灸组均能显著降低高脂血症兔TNF-α及INF-γ的水平(P<0.05),且氮酮组和冰片组降低TNF-α、INF-γ的疗效优于无促透剂组(P<0.05),冰片组降低TNF-α的效果优于氮酮组(P<0.05).结论:隔药饼灸能有效调节高脂模型兔血脂及炎性反应因子水平,氮酮和冰片作为促透剂运用于药饼后施灸对实验性高脂模型兔血脂及炎性反应因子的调节作用更显著,且冰片组作用比氮酮组更优,值得进一步研究.

Objective: To observe the lipid-lowering effect of different transdermal absorption enhancers applied to the herbal cake-partitioned moxibustion in hyperlipidemia model rabbits, and to explore the possible mechanism. Methods: Forty New-Zealand rabbits were randomly divided into 5 groups using the random number table method, with 8 rats in each group. Rabbits in the blank group were fed routinely with normal diet; rabbits in the other groups were fed with high-fat diet for 12 weeks to establish the hyperlipidemia model. Rabbits in the blank and the model groups were not treated. After the model was prepared, rabbits in the non-transdermal absorption enhancer group received herbal cake-partitioned moxibustion without transdermal absorption enhancer; rabbits in the laurocapram group and the borneol group received herbal cake-partitioned moxibustion with laurocapram or borneol respectively. After 4 weeks of treatment, serum was collected for enzyme-linked immunosorbent assay (ELISA), and the liver tissues were isolated for immunohistochemistry, quantitative polymerase chain reaction (qPCR) and Western-blotting (WB) detection. Results: Serum ELISA results showed that leptin was significantly decreased in the model group compared with the blank group (P<0.05); compared with the model group, leptin was significantly increased in the non-transdermal absorption enhancer, the laurocapram and the borneol groups (all P<0.05); compared with the non-transdermal absorption enhancer group, leptin was significantly increased in the laurocapram group and the borneol group (both P<0.05); there was no significant difference in leptin between the laurocapram and the borneol groups (P>0.05). The qPCR results of rabbit liver tissues showed that the mRNA expressions of leptin, Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) in the model group were significantly lower than those in the blank group (all P<0.05); compared with the model group, the mRNA expressions of leptin, leptin receptor (LR), JAK2 and STAT3 in the non-transdermal absorption enhancer, the laurocapram and the borneol groups were significantly increased (all P<0.05); compared with the non-transdermal absorption enhancer group, the mRNA expressions of leptin, LR, JAK2 and STAT3 in the laurocapram and the borneol groups were significantly increased (all P<0.05); compared with the laurocapram group, the mRNA expressions of leptin, LR, JAK2 and STAT3 in the borneol group were significantly increased (P<0.05). The trend of immunohistochemistry and WB detection results was basically consistent with the qPCR assay results. The immunohistochemistry and WB detection results of phosphorylated JAK2 (phospho-JAK2) and phosphorylated STAT3 (phospho-STAT3) were basically consistent with those of JAK2 and STAT3. Conclusion: The molecular expression of Leptin/JAK2/STAT3 pathway in the hyperlipidemia model rabbits was decreased. The molecular expression of Leptin/JAK2/STAT3 pathway was significantly increased after the herbal cake-partitioned moxibustion. The application of laurocapram and borneol, as transdermal absorption enhancers, in the herbal cake-partitioned moxibustion could more obviously up-regulate the factors of the Leptin/JAK2/STAT3 lipid-regulating pathway than the herbal cake-partitioned moxibustion alone.

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目的:通过Meta分析,评价长蛇灸及以长蛇灸为主治疗类风湿性关节炎(RA)的临床疗效.方法:计算机检索中国知网(CNKI)、维普中文科技期刊数据库(VIP)、万方数据及PubMed数据库,时间为自建库至2018年6月15日,检索长蛇灸及以长蛇灸为主治疗RA的临床随机对照试验(RCTs),采用RevMan 5.3进行Meta分析.结果:最终纳入10篇文献,共1287例患者.Meta分析结果显示,长蛇灸治疗RA总有效率优于对照组(P ＜0.00001),长蛇灸+针刺治疗该病总有效率优于对照组(P ＜0.00001),长蛇灸组类风湿因子(RF)、血沉(ESR)、C反应蛋白(CRP)显著低于对照组(P ＜0.00001).结论:长蛇灸及长蛇灸联合针刺治疗RA是有效的,且其疗效优于其他对照疗法,长蛇灸可降低RA患者RF、ESR、CRP水平,且优于其他对照疗法.但由于纳入的文献数量较少,且方法学质量不高,故上述结论尚需进一步验证.

ObjectiveTo observe the effect of moxibustion on T lymphocyte subsets in peripheral blood of rats with gastric cancer.MethodsSixty healthy Sprague-Dawley (SD) rats were adaptively fed for one week. By the random number table method, 10 rats were randomly selected as a blank group, and 12 rats were randomly selected to simulate the tumor transplantation process; after transplantation, 10 rats were randomly selected as a sham operation group. The remaining 38 rats were used to prepare gastric cancer models by gastric transplantation of the Walker-256 tumor tissue; 8 rats were randomly selected to verify the successful modeling after 7 d; the remaining 30 rats were randomly divided into a model group, a moxibustion group and an infrared group by the random number table method, with 10 rats in each group. From the first day of enrollment, the rats in the moxibustion group received mild moxibustion at Zhongwan (CV 12), Guanyuan (CV 4) and bilateral Zusanli (ST 36) (the first group) and bilateral Pishu (BL 20) and Weishu (BL 21) (the second group), and the two groups of acupoints were alternated every other day. The rats in the infrared group received infrared radiation on the stomach area and the area on the back between the T-12-T-13 spinous processes, the two areas were alternated every other day. Rats in the moxibustion group and the infrared group were treated for 20 min each time, once a day for 21 d. Rats in the blank group, the sham operation group, and the model group were simultaneously grasped and fixed, and no other treatment was performed. After 21 d of intervention, the rats in each group were fasted for 12 h, and blood was collected from the orbits. The numbers of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+) T lymphocytes in peripheral blood were determined by flow cytometry, and the ratio of CD3(+)CD4(+)/CD3(+)CD8(+) was calculated. The rats were sacrificed and the thymus was dissected under sterile conditions to calculate the thymus index.ResultsCompared with the blank group, the thymus index, peripheral blood CD3(+), CD3(+)CD4(+), CD3(+)CD8(+) and CD3(+)CD4(+)/CD3(+)CD8(+) ratio in the sham operation group did not change significantly (all P>0.05). Compared with the blank group, the thymus index of the model group was increased (P<0.05), the CD3(+) and CD3(+)CD8(+) T lymphocytes were increased (both P<0.01), and the CD3(+)CD4(+)/CD3(+)CD8(+) ratio was decreased (P<0.05). Compared with the model group, the thymus index of the moxibustion group was increased (P<0.01), and CD3(+), CD3+CD4+ and CD3(+)CD8(+) T lymphocytes and the ratio of CD3(+)CD4(+)/CD3(+)CD8(+) in peripheral blood were increased (all P<0.05). Compared with the infrared group, the thymus index of the moxibustion group was significantly increased (P<0.05), the CD3(+) and CD3(+)CD4(+) T lymphocytes in the peripheral blood were significantly increased (both P<0.01), and the CD3(+)CD8(+) was increased (P<0.05).ConclusionMoxibustion can significantly increase the thymus index of gastric cancer-bearing rats and activate CD3(+)CD4(+) and CD3(+)CD8(+) T lymphocytes in peripheral blood.

目的:观察隔药饼灸对慢性支气管炎大鼠外周血中中性粒细胞(PMN)的影响,以阐明其作用机制,为隔药饼灸防治慢性支气管炎提供实验依据.方法:将48只SD大鼠随机分为空白组、模型组、核酪口服液组、穴位隔药饼灸4组,每组12只.观察大鼠的一般情况,用流式细胞术检测各组血清中中性粒细胞吞噬指数.结果:与模型组比较,穴位隔药饼灸和核酪口服液组PMN吞噬指数均有降低(P<0.05),且穴位隔药饼灸组降低效果更优(P<0.05).结论:穴位隔药饼灸能改善慢支大鼠肺与气管组织损伤情况并控制炎症,其机制可能是通过降低大鼠PMN吞噬指数,起到有效控制炎症作用.

目的 观察艾灸对胃荷瘤大鼠瘤体增长的抑制作用.方法 50只健康SD大鼠,随机分为空白组、假手术组、模型组、艾灸组、红外组5组,每组10只.采用Walker-256细胞实体瘤组织移植胃部制备动物模型.成模后,艾灸组、红外组分别施以温和灸、红外线治疗20 min,每日1次,持续21 d.实验期间,观察大鼠的生存状态,记录饮食、体重等变化.治疗结束后,摘取胃部,测量肿瘤体积、计算生长抑制率.采用免疫组化法,观测肿瘤细胞增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)的表达,TUNEL观测肿瘤细胞凋亡.结果 实验期间,模型组动物生存状态持续变差,成模5 d后生存状态积分明显低于空白组(P<0.01);艾灸组动物生存状态自治疗10 d后开始改善,治疗15 d后积分明显高于模型组(P<0.01).治疗结束后,与模型组比较,艾灸组瘤体增长抑制率达41.89％,瘤体内出现大量坏死灶,血管较少,肿瘤细胞增殖减少、凋亡增多(P<0.01).与红外组比较,艾灸组在改善生存状态、抑制肿瘤体积增长、减少肿瘤细胞增殖及转移、促进肿瘤细胞凋亡上作用更明显(P<0.05).结论 艾灸可明显改善荷瘤大鼠的生存状态,通过减少肿瘤细胞的增殖、促进肿瘤细胞的凋亡以抑制瘤体的增长.

目的 探讨氮酮和冰片作为促透剂运用于隔药饼灸对高脂血症合并动脉粥样硬化兔主动脉病理形态及血清炎症因子的影响.方法 选取40只新西兰纯种兔随机分成5组,每组8只,即正常组、模型组、无促透剂组、氮酮组和冰片组.高脂血症合并动脉粥样硬化兔模型造模成功后除正常组和模型组外,其余各组采用隔药饼灸干预,每日1次,连续4周.采用ELISA检测血清白细胞介素6(Interleukin 6,IL-6)、白细胞介素10(Interleukin 10,IL-10)含量;采用HE染色,光学显微镜下观察主动脉组织形态.结果 与正常组比,模型组IL-6水平显著升高、IL-10水平显著降低(P<0.01);与模型组比,无促透剂组、氮酮组及冰片组IL-6水平显著降低(P<0.01)、IL-10水平升高(P<0.05);与无促透剂比,氮酮组和冰片组IL-6水平显著降低(P<0.01)、IL-10差异无统计学意义(P>0.05);与氮酮组比,冰片组IL-6水平显著降低(P<0.01)、IL-10差异无统计学意义(P>0.05).主动脉组织镜检结果示:正常组无异常;模型组可见组织整体凌乱,管腔壁可见明显增厚,内皮坏死,有明显的脂质斑块及泡沫细胞;无促透剂组有内膜增厚,可见少量泡沫细胞,细胞排列较模型组稍好,脂质沉积明显较模型组减少,斑块不明显;氮酮组和冰片组无显著异常.结论 隔药饼灸对高脂血症合并动脉粥样硬化兔具有抑制炎症反应和抗动脉粥样硬化作用,在药饼中加入促透剂后施灸疗效更优,可能与药饼中药物透皮吸收增强有关,值得进一步临床应用和研究.

根据《关于医教协同深化临床医学人才培养改革的意见》要求,我校已从2015级起对中医硕士专业学位研究生执行培养和住培并轨合一.近2年的培养过程中,我校研究生教育工作者在实践过程中发现问题、解决问题,不断探索培养新模式,形成系统的改革思路与完善的制度支撑,进一步提高了中医硕士专业学位研究生培养质量,开展了全面、系统、科学的培养综合改革.

Objective To observe the clinical efficacy of acupuncture plus medication in treating chronic pelvic inflammatory disease. Method By using the random number table, sixty-eight patients with chronic pelvic inflammatory disease were randomized into an acupuncture-medication group of 34 cases and a medication group of 34 cases. The clinical efficacies were compared after 2 courses of treatment, and the symptoms and body signs scores and syndrome score of traditional Chinese medicine (TCM) were also compared. Result There was a significant difference in comparing the therapeutic efficacy between the acupuncture-medication group and the medication group (P<0.05). After the treatment, the symptoms and body signs scores and TCM syndrome score dropped significantly in both groups (P<0.05), indicating that the two groups both had improvement in the symptoms, body signs and TCM syndrome; there were significant between-group differences in comparing the score differences in the symptoms and body signs scores and TCM syndrome score after the treatment (P<0.05), and the acupuncture-medication group was higher than the medication group. Conclusion Acupuncture plus medication can better ameliorate the symptoms and body signs and TCM syndrome in chronic pelvic inflammatory disease.

Objective To observe the effect of herbal cake-separated moxibustion on the content of tumor necrosis factor-α (TNF-α) protein and its mRNA in the lung tissue of rats with chronic bronchitis (CB). Methods Forty-eight Sprague-Dawley rats were randomly and equally divided into four groups, including blank group, model group, herbal cake-separated moxibustion group, and paper pad-separated moxibustion group. CB model rats were prepared by modified fumigation. The general status of rats after herbal cake-separated moxibustion was observed and compared with that in the other three groups. The expression of TNF-α mRNA in rat lung was measured by RT-PCR, and the protein expression of TNF-α in lung tissues was measured by Western blot.Results The pathological injury of lung and trachea of rats in herbal cake-separated moxibustion group and paper pad-separated moxibustion group was improved compared with that in the model group. Herbal cake-separated moxibustion and paper padseparated moxibustion can reduce the expression of TNF-α mRNA and TNF-α protein in the lung tissues of CB rats (P＜0.05), and the effect of herbal cake-separated moxibustion was better (P＜0.05). Conclusion Herbal cake-separated moxibustion can improve the general symptoms and airway inflammation of CB rats, possibly by down-regulating the expression of TNF-α protein and its mRNA.

随着信息时代的到来,互联网在各行各业中产生了巨大的影响.微课作为互联网催生的新型教学模式及教学方法,在中医药高等院校当中有了具体的运用及创新.本文通过回顾微课在《实验针灸学》教学过程中的具体运用情况,分析比较微课与传统课程在教学效果等方面的差异,探索《实验针灸学》微课教学的具体模式,以期推广微课,让当代大学生从中获益.

针刺治疗是针具和手法的结合.在针刺得气后,为达到一定的治疗目的,往往需要施展一定的手法.“青龙摆尾”针刺手法源于明代医家徐凤的“飞经走气”四法之一,该手法自创立以来在针灸界影响颇大,深受后世医家的重视,许多医家根据临床实践在此基础上进行了创新.笔者通过查阅文献,总结历代医家对该手法的论述及现今临床运用情况,并提出自己的一些看法.