Jujube is a medicinal herb and daily food. Polysaccharides extracted from jujube possesses immune regulatory activity. In this study, we investigated whether jujube polysaccharides (JP) could ameliorate ulcerative colitis (UC), an inflammatory disease, and explore the potential mechanism of UC. We first extracted and characterized the structure JP, using dextran sulfate sodium-induced UC mice model to verify the pharmacological function. Research proved that JP treatment could ease UC mice symptoms and pathological injury. According to transcriptomics analysis, majority genes were enriched to cytokines-cytokines receptors signal pathway. Results showed that the increasing proinflammatory cytokines in UC mice colon were declined after JP intervention. Meanwhile, the receptor of interleukin-33 (IL-33), tumorigenicity 2 suppression (ST2) and downstream protein MYD88 expression in UC mice colon were corrected by JP intervention. As a result, according to immunohistochemistry detection, JP downregulated UC mice colon T cells expression (labeled by CD3+). We further employed flow cytometry to validate that JP could suppress CD4+ high expression on Jurkat cell stimulated by IL-33. In conclusion, JP could ameliorate UC mice symptoms, the mechanism of which was related to the regulation of IL-33/ST2 axis.

辣木叶来源于热带植物辣木Moringa oleifera,以叶入药,具有良好的生理活性.现代研究显示辣木叶含有丰富的黄酮、多糖、多酚、生物碱等功能成分,具有明确的抗氧化、抗菌、降糖、抗高脂血症等药理作用.辣木叶及其有效成分可能通过抑制脂质过氧化、抑制胰脂肪酶活性、抑制α-葡萄糖苷酶活性、诱导棕色脂肪组织生成、提高胆汁酸结合能力等途径达到抗高脂血症作用.综述辣木叶及其有效成分抗高脂血症药理作用的研究进展,以期为利用该药用植物资源开发新药提供依据.

目的 探讨辣木叶水提物(MOLAE)对脂肪堆积人HepG2细胞的调节作用.方法 制备MOLAE冻干粉;通过CCK-8法检测油酸钠-钠棕榈酸酯(O-P)对HepG2细胞活力的影响、油红O染色检测O-P对细胞脂质堆积的影响、试剂盒法检测O-P对细胞三酰甘油(TG)、总胆固醇(TC)水平的影响,筛选O-P诱导HepG2细胞脂质代谢异常模型的作用浓度及时间;CCK-8法检测辛伐他汀、MOLAE对HepG2细胞活力的影响,筛选安全作用浓度;设立对照组、模型组、辛伐他汀(阳性药,15 μmol·L-1)组和MOLAE(3.125、6.250、12.500、25.000、50.000 μg·mL-1)组,除对照组外,其他各组均给予0.4-0.2 mmol·L-1的O-P诱导细胞脂质沉积,诱导3 h后开始给药,干预24h后,CCK-8法检测细胞活性,油红O染色观察细胞中脂滴形成情况,试剂盒法测定细胞中TG、TC、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)及丙二醛(MDA)水平.结果 确定造模方式为:0.4-0.2mmol·L-1的O-P作用HepG2细胞3h后开始给药;10、15μmo1·L-1的辛伐他汀和3.125～100.000μg·mL-1的MOLAE作用于HepG2细胞24、48h后,不影响细胞活力.与模型组比较,不同浓度的MOLAE(3.125、6.250、12.500、25.000、50.000 μg·mL-1)均能降低TG、TC、MDA水平(P＜0.05、0.01),显著升高GSH、SOD水平(P＜0.05、0.01).油红O染色结果表明,辛伐他汀组和MOLAE组(12.5、25.0μg·mL-1)脂滴堆积现象均较模型组有明显改善.结论 MOLAE能够降低O-P诱导的HepG2细胞中TG、TC水平,提高GSH、SOD水平和降低MDA水平,减少细胞中脂质堆积的现象.

目的 采用UPLC-Q-TOF/MS技术对加味左金丸中的化学成分进行定性分析.方法 色谱柱为ACQUITY Premier BEH C18(100mm×2.1mm,1.8 pm),流动相为乙睛-甲醇-0.1％甲酸水溶液,梯度洗脱,体积流量为0.3 mL/min;质谱的离子源为ESI,模式为正、负离子模式.通过质谱给出的分子离子峰和二级质谱碎片离子峰,结合对照品的相对保留时间,参考相关的文献,对于加味左金丸中的化学成分进行鉴定.并建立不同批次加味左金丸样品的指纹图谱.结果 通过对数据的分析,从加味左金丸中鉴定出92个成分,其中包括16个有机酸类成分、34个黄酮类成分、17个生物碱类成分、21个萜类成分与4个其他类成分.建立的加味左金丸指纹图谱有13个共有峰,分别为黄芩苷、山柰酚-3-O-葡萄糖苷、槲皮素、汉黄芩素、甘草素、千层纸素A、芍药苷、吴茱萸次碱、小檗碱、巴马汀、枸橘苷、橙皮苷、柴胡皂苷d.指纹图谱方法的精密度、稳定性、重复性良好,多批次加味左金丸的成分基本一致.结论 建立的UPLC-Q-TOF/MS方法对化学成分进行了鉴定,建立的指纹图谱方法为进一步分析加味左金丸的物质基础提供了重要依据.

Ethnopharmacological relevance: Jian-Pi-Yi-Shen (JPYS) is a herbal decoction being used to relieve the symptoms of chronic kidney disease (CKD) and its complications, including anemia, for over twenty years. Nonetheless, it is unclear how JPYS influences renal anemia and iron metabolism. Aim of the study: An analysis of network pharmacology, chemical profiling, and in vivo experiments was conducted to identify the impact of JPYS on JAK2-STAT3 pathway and iron utilization in renal anemia and CKD. Materials and methods: The chemical properties of JPYS and its exposed ingredients were detected in vivo. And based on the aforesaid chemical compounds, the potential targets and signaling pathways of JPYS for renal anemia treatment were predicted by network pharmacology. Afterward, an adenine-feeding animal model of CKD-related anemia was developed to verify the mechanism by which JPYS modulates iron recycling to treat renal anemia. Renal injury was estimated by serum creatinine (Scr), blood urea nitrogen (BUN), histopathological examinations and fibrosis degree. Western blot, enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry approaches were utilized to assess the levels of JAK2, STAT3 and iron metabolism-related factors. Results: There were 164 active ingredients identified in JPYS, including prototypes and metabolites in vivo were identified in JPYS, and 21 core targets were found through network pharmacology based on topological characteristics. Combined with the core targets and pathway enrichment analysis, the majority of the candidate targets were associated with the JAK2-STAT3 signaling pathways. Experimental results indicated that JPYS treatment significantly decreased the expression of BUN and Scr, restored renal pathological damage, downregulated fibrosis degree, and improved hematological parameters such as red blood cell, hemoglobin and hematocrit in CKD rats. Furthermore, JPYS significantly restored iron metabolism from dysregulation by increasing the levels of iron and ferritin in the serum, inhibiting the production of hepcidin in liver and serum, and regulating transferrin receptor 1 in bone marrow. Meanwhile, the expression of JAK2 and STAT3 was suppressed by JPYS treatment. Conclusions: Based on these results, JPYS reduces hepcidin levels by inhibiting the activation of JAK2-STAT3 signaling, thereby protecting against iron deficiency anemia.

Helicobacter pylori was classified by the World Health Organization as a class 1 carcinogen. The development of drug-resistant strains of this pathogen poses a serious threat to human health worldwide. The cell invasion of H. pylori activates xenophagy in gastric epithelial cells by mediating miR-30b/c, and the emergence of autophagosomes provides a niche that enables the survival of intracellular H. pylori and promotes its drug resistance. This study revealed that some clinical drug-resistant H. pylori strains present much stronger invasive ability than standard strains. Patchouli alcohol (PA), a tricyclic sesquiterpene from Pogostemon cablin (Blanco) Benth (Labiatae), showed reliable activity against intracellular H. pylori . The mechanisms appeared to involve the downregulation of miR-30c-3p/5p and miR-30b-5p, thereby upregulating xenophagy-related gene expression (ULK1, ATG5, ATG12, and ATG14) and enhancing xenophagy. PA also inhibited the nuclear transfection of miR-30b-5p induced by H. pylori , thereby enhancing transcription factor EB function and increasing lysosome activity. The finding of strongly invasive intracellular H. pylori has great implications for clinical treatment, and PA can act against invasive H. pylori based on the improvement of miR-30b/c mediated xenophagy. Taken together, the results demonstrate that PA have potential use as a candidate medication for intracellular drug-resistant H. pylori .

目的 通过电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP-MS)法建立加味左金丸中Cd、Pb、As、Hg、 Co、V、Ni、Cu、Li、Sb、Ba、Mo、Sn、Cr、Na、Mg、 Al、Ca、Ti、Mn、Fe、Zn、Ga、Se、Sr、Tl共计26种无机元素的测定方法.方法 加味左金丸通过微波消解法处理后,根据相对分子质量的大小选择内标物,其中7Li、23Na、24Mg、 27Al、40Ca、48Ti、51V、52Cr、55Mn、56Fe、58Ni、59Co、63Cu、66Zn、70Ga、75As、77Se、86Sr以72Ge作为内标;95Mo、114Cd、118Sn、121Sb、137Ba以115In作为内标;202Hg、 205T1、208pb以209Bi作为内标.对标准品溶液、空白溶液与供试品溶液进行分析,采用标准曲线法进行定量分析.通过ICP-MS法进行测定.结果 26种无机元素线性的相关系数r≥0.9996,检出限为0.001～1.500 μg/L,定量限为0.01～5.00 μg/L,精密度与重复性试验的RSD均小于5％,平均回收率在82.64％～106.44％,RSD均小于5％.对3个厂家的12批样品进行了测定,26种元素的含量差异较大,其中Na、Mg、 Ca、Fe4种元素的含量比较高,均大于500μg/g,Cd、Pb、As、Hg、 Co、Li、Sb、Mo、Sn、Cr、Se、 T1的含量比较低,均小于1μg/g.由结果可知,人体的常量元素,如Na、Mg、 Ca的含量比较高,Cd、Pb、As、Hg等有害元素含量比较低.根据《中国药典》2020年版一部的要求,本品中Cd、Pb、As、Hg与Cu均符合规定.结论 该方法快速、准确,可以用于加味左金丸中无机元素的测定.

左金方为元朝医家朱震亨创制的经典中药复方,始载于《丹溪心法》,仅由黄连、吴茱萸以6∶1比例组方,治疗肝火犯胃所致胁肋胀痛、吞酸呕吐等,如今不仅用于多种消化系统病症(如胃溃疡、胃炎、胃癌、幽门螺杆菌感染等),还在精神症状、非胃肠道肿瘤、高血压、糖尿病等非消化系统疾病的治疗中起到较好疗效,具有抗炎抗溃疡、抗肿瘤、抗菌等多种作用.就近5年来对左金方的化学成分与药理作用的研究进展进行归纳总结,同时对其未来可能的研究方向进行了展望,以期为其进一步综合开发利用奠定基础.

Jian-Pi-Yi-Shen (JPYS), the traditional Chinese medicine (TCM) decoction, has been commonly used to treat chronic kidney disease (CKD) and its complications such as anemia. JPYS has been previously found to induce erythropoietin (EPO) production in HEK293T cells and CKD rats. However, the mechanism of JPYS in treating anemia of CKD rats has remained largely unknown. Here, we further extend our effort to investigate the translational control of hypoxia inducible factor- (HIF-) α protein via ERK signaling and the effect on iron recycling-related protein expression by JPYS, thus revealing the mechanism of JPYS in correcting anemia in CKD. Experimental CKD rats with anemia were induced by 5/6 nephrectomy. Rats were administrated orally with high dose (6.0 g/kg/d) and low dose (1.5 g/kg/d) of JPYS for 90 days. Serum hepcidin level was determined to evaluate iron homeostasis. The protein expressions of HIF-2α, erythropoietin (EPO), ferritin, and ferroportin (FPN) and the phosphorylation level of extracellular signal-regulated kinase 1/2 (ERK1/2) were detected by Western blot. The results showed that JPYS treatment significantly ameliorated kidney function by reducing increased levels of blood urea nitrogen (BUN), serum creatinine (Scr), and urine protein (UPRO). Periodic acid-Schiff (PAS) and Masson staining observation showed that the renal pathological damage was restored in JPYS-treated CKD rats. In parallel, JPYS markedly improved CKD anemia through upregulation of red blood cell (RBC), hemoglobin (HGB), and hematocrit (HCT). JPYS stimulated EPO and HIF-2α protein expressions in both the kidney and liver of CKD rats. Furthermore, JPYS induced the phosphorylation of ERK1/2 protein. In addition, JPYS regulated protein expression of ferritin and FPN in both the liver and spleen of CKD rats and the serum level of hepcidin. In conclusion, JPYS induces the expression of EPO through ERK-mediated HIF-2α protein accumulation and regulates systemic iron recycling, supporting its role in promoting erythropoiesis and improvement of anemia in CKD.

目的 建立了HPLC-ESI-MS/MS法同时测定加味左金丸中阿魏酸、木香烃内酯、黄芩苷、芍药苷、延胡索乙素、吴茱萸次碱、小檗碱、巴马汀、吴茱萸碱、柚皮苷、橙皮苷、柴胡皂苷a与柴胡皂苷d13种有效成分的分析方法.方法 HPLC采用色谱柱Thermo Syncronis C18柱(100 mm×4.6 mm,3.0 μm),流动相为甲醇-0.02 mol/L乙酸铵水溶液,梯度洗脱,体积流量0.35 mL/min,进样量10μL;质谱采用电喷雾离子源、正负离子模式同时采集,通过多反应监测(MRM)同时对加味左金丸中的13种有效成分进行定量分析.结果 加味左金丸中13种有效成分阿魏酸、木香烃内酯、黄芩苷、芍药苷、延胡索乙素、吴茱萸次碱、小檗碱、巴马汀、吴茱萸碱、柚皮苷、橙皮苷、柴胡皂苷a与柴胡皂苷d的线性范围分别为2～80 μg/mL (r=0.999 2)、0.5～20.0 μg/mL (r=0.999 1)、3.5～140.0 μg/mL (r=0.999 8)、1～40 μg/mL (r=0.999 2)、0.3～12.0 μg/mL (r=0.999 1)、1～40 μg/mL (r=0.999 2)、3～120 μg/mL (r=0.999 7)、2.5～100.0 μg/mL (r=0.999 5)、0.5～20.0 μg/mL(r=0.999 3)、0.5～20.0 μg/mL (r=0.999 1)、1～40 μg/mL (r=0.999 1)、0.3～12.0 μg/mL (r=0.999 2)、0.3～12.0 μg/mL (r=0.999 2),平均加样回收率分别为99.5％、98.9％、100.2％、99.2％、99.5％、99.7％、98.6％、99.9％、101.3％、98.7％、99.8％、97.9％、101.3％,RSD值分别为4.11％、4.88％、1.08％、3.23％、4.13％、3.23％、2.78％、3.12％、4.53％、3.43％、3.58％、5.22％、5.13％.12批加昧左金丸样品中13种有效成分的质量分数分别为0.324～0.383、0.051～0.072、3.225～3.466、0.154～0.198、0.015～0.062、0.144～0.199、2.145～2.982、0.441～0.953、0.032～0.099、0.062～0.089、0.111～0.178、0.012～0.065、0.011～0.069 mg/g.结论 所建立的分析方法快速、高效、灵敏度高、专属性好,可应用于加味左金丸的质量控制.

目的:观察左金方治疗幽门螺杆菌感染相关性胃病的临床疗效.方法:采用随机数字表法将幽门螺杆菌感染相关性胃病患者90例分为两组各45例,对照组予以标准三联方案(奥美拉唑+阿莫西林+克拉霉素)治疗,治疗组采用左金方+奥美拉唑治疗,比较两组患者中医症状评分、幽门螺杆菌根除率及不良反应发生率.结果:治疗组患者中医症状评分均较治疗前显著降低(P＜0.05),与对照组比较,差异有统计学意义(P＜0.05);14C呼气试验显示,治疗组、对照组患者幽门螺杆菌根除率分别为73.33％、80.00％,组间差异无统计学意义(P＞0.05);治疗组不良反应发生率(6.67％)较对照组(26.67％)显著降低,差异有统计学意义(P＜0.05).结论:左金方具有根除幽门螺杆菌效果,与标准三联治疗效果相当,并可有效改善患者中医症状评分,降低不良反应发生率.

Objective To compare the inhibitory effect of formula granule, single and mixed decoction of Huanglian and Wuzhuyu at different proportions on Helicobacter pylori (H.pylori) and the equivalence of formula granule and traditional decocting. Methods The concentration of bacteria was determined by Maxwell Turbidimetry (MAE), and the growth curve of bacteria was obtained. The minimum inhibition concentration (MIC) of formula granule and traditional decoction of Huanglian, and Wuzhuyu at different proportions (6∶1, 5∶2, 4∶3, 3∶4, 2∶5, and 1∶6) was measured by agar dilution method. Results After the bacteria were treated with of formula granules and traditional decoctions for 48 h respectively, Hp SS1 was taken as example, the MIC was confirmed as 0.900 mg·mL-1 for Huanglian granules and 0.869 mg·mL-1 for its traditional decoction, while 1.140 mg·mL-1 for Wuzhuyu granules and 1.118 mg·mL-1 for its traditional decoction, with no difference between the granules and their decoctions (P＞ 0.05). The MIC was 0.810 mg·mL-1 for granules of Huanglian and Wuzhuyu at 6∶1 and 0.396 mg·mL-1 for granules of Huanglian and Wuzhuyu at 1∶6, 1.800 mg·mL-1 and 0.818 mg·mL-1 for their corresponding decoctions. The inhibition of Huanglian and Wuzhuyu at 6∶1 was the strongest, regardless of the formula granules or traditional decoctions, and the effects of traditional decoctions outweighed their formula granules (P ＜ 0.01). Conclusion H.pylori can be effectively inhibited by formula granule, single and mixed decoction of Huanglian and Wuzhuyu at different proportions. Proportions at 6∶1 has the best compatibility. and traditional decoctions have better effect than their formula granules. This experiment provides a experimental basis for the equivalence and clinical application between formula granule and decoction.

目的 研究左金方对幽门螺旋杆菌引起人正常胃黏膜上皮(GES-1)细胞增殖与凋亡的影响.方法 幽门螺旋杆菌以不同的感染复数(1∶1、50∶1、100∶1、200∶1、300∶1)感染GES-1细胞,于12、24、48h后,CCK-8检测细胞的增殖活性;以不同质量浓度(0.5、1.0、2.0、4.0μg/mL)左金方作用幽门螺旋杆菌感染的GES-1细胞12、24、48 h后,收集细胞,CCK-8检测细胞的增殖活性,流式细胞仪和Western blotting检测细胞的凋亡率;Hochest染色检测凋亡细胞的形态学改变.结果 幽门螺旋杆菌感染GES-1细胞后,感染复数越大,侵染时间越长,抑制细胞生长和促进细胞凋亡的作用越强,幽门螺旋杆菌以100∶1的感染复数作用人GES-1细胞12、24、48h后,细胞存活率分别降低到(80.57±1.21)％、(70.04±3.21)％、(67.74±2.91)％,细胞的凋亡率分别增加到(23.74±1.71)％、(53.60±1.87)％、(70.67±2.87)％;与1.0μg/mL左金方共培养24 h后,细胞存活率上升到(97.67±1.04)％,细胞凋亡率降低到(31.04±1.02)％,与相应模型组对比,差异显著(P＜0.01);Western blotting结果表明,左金方各处理组细胞Caspase-3蛋白水平表达均明显降低;Hochest荧光染色,从形态学的角度进一步验证了这一结果.结论幽门螺旋杆菌感染人GES-1细胞后,可以抑制细胞增殖,诱导细胞凋亡,左金方可以降低幽门螺旋杆菌引起的细胞损伤,保护GES-1细胞.

SMG-1,a member of the phosphoinositide kinase-like kinase family, functioned as a tumor suppressor gene. However, the role of SMG-1 in GC remain uncharacterized. In this study, regulation of SMG-1 by miR-192 and-215, along with the biological effects of this modulation, were studied in GC. We used gene microarrays to screening and luciferase reporter assays were to verify the potential targets of miR-192 and-215. Tissue microarrays analyses were applied to measure the levels of SMG-1 in GC tissues. Western blot assays were used to assess the signaling pathway of SMG-1 regulated by miR-192 and-215 in GC. SMG-1 was significantly downregulated in GC tissues.The proliferative and invasive properties of GC cells were decreased by inhibition of miR-192 and-215, whereas an SMG-1siRNA rescued the inhibitory effects. Finally, SMG-1 inhibition by miR-192 and-215 primed Wnt signaling and induced EMT. Wnt signaling pathway proteins were decreased markedly by inhibitors of miR-192 and-215, while SMG-1 siRNA reversed the inhibition apparently. Meanwhile, miR-192 and-215 inhitibtors increased E-cadherin expression and decreased N-cadherin and cotransfection of SMG-1 siRNA reversed these effects. In summary, these findings illustrate that SMG-1 is suppressed by miR-192 and-215 and functions as a tumor suppressor in GC by inactivating Wnt signaling and suppressing EMT.

目的:观察四神丸治疗脾肾阳虚型肠易激综合征的疗效.方法:48例脾肾阳虚型肠易激综合征患者,随机分为A组和B组,各24例.A组口服四神丸汤药治疗,B组常规口服培菲康治疗.比较两组疗效.结果:A组总有效率为83％,B组总有效率为67％,两组总有效率比较差异有统计学意义(P＜0.05).结论:四神丸对脾肾阳虚型肠易激综合征患者脾胃功能恢复有良好效果.

Objective:To study the antimicrobial activities of ZuoJinFang against helicobacter pylori in vivo and in vitro.Methods:The minimum inhibiting concentration (MIC) of ZuoJinFang and Clarthromycin were measured by agar dilution method;sixty BALB/c mice,half male and half female,were randomly divided into 6 groups,normal control group,model group,gastric triad group (0.351 mg· g-1) and ZuoJinFang low,middle and high dose groups (0.364,0.728,1.456 g· kg-1 · d-1),the mice were inoculated orally with antibiotic on first day and with Helicobacter pylori strains for 7 continuous days to establish Hp infection model.From experimental day 11,the mice were given oral administration of the corresponding drugs for 8 weeks.At the end of the experiment,gastric mucosa and serum were obtained for serum HP antibody test and pathological section examination.Results The MIC of ZuoJinFang was confirmed as 200 μg· mL-1 and that of Clarthromycin was 0.5 μg· mL-1.The result of serum antibody test and pathological section examination showed that Hp infection model had been established successfully,compared with the normal control,were statistically significant.And both gastric triad group and ZuoJinFang could inhibit the growth of Hp in vivo,compared with the model group,were statistically significant.Conclusion:ZuoJinFang are effective on inhibiting the growth of Hp in vivo and in vitro.

目的:研究左金方和5-FU单独与联合对人正常胃黏膜上皮细胞GES-1和人胃癌MGC803细胞生长及凋亡的影响及对BAX/Bel-2蛋白表达水平的改变.方法:将不同浓度的左金方、5-FU及两药联合作用于体外培养的人胃黏膜上皮细胞GES-1和人胃癌细胞MGC803,Cell Counting Kit-8 (CCK-8)检测细胞增殖率的变化,流式细胞仪检测细胞凋亡,Western blot法检测bel-2、bax蛋白表达.结果:1.00 ug·mL-1左金方、50.00、100.00 ug· mL-1 5-FU作用GES-1细胞24 h后,细胞存活率分别为(107.54±2.25)％、(53.67±1.91)％和(47.43±1.35)％,作用MGC803细胞24 h后,细胞凋亡率分别为(45.46±1.66)％、(37.02±1.68)和(45.60±1.14)％;左金方联合5-FU (1.00+50.00)、左金方联合5-FU (1.00 +100.00)作用GES-1细胞24 h后,细胞存活率分别为(81.66±2.22)％和(78.68±2.10)％,作用MGC803细胞24 h后,相应的凋亡率为(61.30±1.71)％和(71.30±1.79)％,与单独用药比较差异有统计学意义.Western Blot凝胶电泳图可以看出,左金方联合5-FU (1.00+50.00)、左金方联合5-FU(1.00+ 100.00)作用MGC803细胞24 h后,进一步促进Bax的表达,降低Bel-2的表达,与各单独用药组比较差异有统计学意义.结论:左金方与5-FU联合用药有大幅降低5-FU细胞毒性作用,显著提高人正常胃黏膜上皮细胞GES-1的存活率;通过促进MGC803细胞中Bax蛋白的高表达和Bel-2的低表达,促进人胃癌细胞的凋亡.

Abstract The aim of this study was to investigate whether and at which neoplastic stage promoter hypermethylation of Helicase-like Transcription Factor (HLTF) is involved in human esophageal carcinogenesis. The researchers examined HLTF promoter hypermethylation using real-time quantitative methylation-specific PCR in 229 primary human esophageal tissues of contrasting histological types. Both HLTF mean normalized methylation value (NMV) and hypermethylation frequency were significantly higher in dysplastic Barrett’s esophagus (Dÿ 0.0303 and 10.0%), and esophageal adenocarcinomas (EAC, 0.0079 and 10.4%) than in normal esophagus (NE, 0.0006 and 0.0%; p<0.05 and p<0.05, respectively). Incremental increases in the frequency of HLTF hypermethylation were observed during progression from NE (0.0%) to Barrett’s esophagus (BE, 3.3%), D (10.0%), and EAC (10.4%). Meanwhile, HLTF mean NMV was significantly higher in esophageal squamous cell carcinoma (ESCC, 0.0102) than in NE (p<0.05). Also, HLTF was hypermethylated in 7.7% ESCCs. Furthermore, mean NMV of HLTF was significantly higher in current alcohol drinking EAC patients (0.0194) than in non-current ones (0.0066, p<0.05). HLTF hypermethyaltion is an uncommon event in human esophageal cancer, but occurs early in a subset of EAC, and is related to the alcohol drinking status of EAC patients.

为了解过程性考核教学方法在消化系统教学过程中的应用情况,在深圳大学医学部2个年级共60名临床医学专业本科生中,分别采用传统的终结性考核模式和改革后的过程性考核模式对消化系统的教学效果进行观察.使用过程性考核方式的班级学生成绩明显高于传统考核方式,并且这种考核方式得到学生的认可和喜爱.过程性考核教学方法可以对学生综合能力进行更全面、更科学的评价,同时有助于培养学生自主学习、分析问题、解决问题的能力.

目的:研究左金方联合5-FU抑制胃癌RF-48细胞增殖,探讨其协同增效作用.方法:Cell Counting Kit-8检测细胞增殖率的变化,流式细胞仪检测细胞凋亡,Hoechst 33258染色观察细胞形态学变化.结果:左金方对HFE145细胞活力的影响很小,而小剂量的5-FU会引起较大的细胞毒性,不同浓度左金方、5-FU均能诱导RF-48细胞的凋亡;左金方联合5-FU后对HFE145细胞存活率的影响,以及对RF-48细凋亡率的影响,与单独用药组比较差异有统计学意义,Hochest染色RF-48细胞出现核固缩状和颗粒状荧光等典型的凋亡学特征.结论:左金方与5-FU联合用药大幅降低了5-FU的细胞毒性作用,显著提高了HFE145细胞的存活率,促进了人胃癌细胞RF-48的凋亡,比左金方或者5-FU单独用药对胃癌的治疗效果更好.