OBJECTIVE:To investigate the expression of ribonucleotide reductase M2 (RRM2) in patients with multiple myeloma and its mechanism of inhibiting human multiple myeloma cell proliferation.METHODS:Thirty-Six patients with multiple myeloma in our hospital from July 2016 to September 2018 were selected as MM group, at the same time simple iron deficiency anemia patients were taken as the control group. RT-qPCR and Western blot techniques were used to determine the mRNA and protein expression of RRM2 in bone marrow mononuclear cells, respectively. siRNAs oligos targeting RRM2 was transfected into RPMI8226 cells to establish the RRM2 silence model. CCK-8 and flow cytometry were used to analyze the cell proliferation and the cell cycle, and Western blot was used to detect the expression of cell cycle related proteins.RESULTS:The expression level of RRM2 mRNA and the expression level of RRM2 protein in multiple myeloma group were significantly higher than those in control group (P＜0.05). Further analysis indicated that the level of RRM2 expression closely correlated with ISS staging, bone destruction and extramedullary infiltration (P＜0.05). Transfection with siRNAs targeting RRM2 could significantly down regulate the RRM2 expression. And the RRM2 down regulation inhibited the cell proliferation and arrested the cell cycle at S stage (P＜0.05). Further study indicated that RRM2 silencing influenced cell cycle-related proteins expression.CONCLUSION:RRM2 overexpressies in bone marrow mononuclear cells of MM patients, moreover significantly relates with the degree of malignancy. The silencing RRM2 in multiple myeloma cells can inhibit cell proliferation through arresting cell cycle at S phase. RRM2 may be a marker to predict the prognosis of patients with multiple myeloma and novel target for new drug development.

Objective To study the expression of CD28/B7 family costimulatory molecules mRNA in bone marrow mononuclear cells of patients with immune-related pancytopenia(IRP),and to explore the role of CD28/B7 family costimulatory molecules in the pathogenesis of IRP. Methods The expressions of cytotoxic T lymphocyte-associated antigen 4(CTLA-4),CD28,CD80 and CD86 mRNA in CD28/B7 family costimulatory molecules were detected by real-time PCR and RNA extraction. Differences in mRNA expression of CD28/B7 family costimulatory molecules were analyzed between the IRP disease group and the iron deficiency poor(IDA)control group. Results The mRNA expression of CD28/B7 family costimulatory molecules was different in IRP and IDA bone marrow mononuclear cells. The expression of CTLA-4 mRNA in bone marrow mononuclear cells of IRP patients was significantly lower than that in IDA group(P<0.01). The expressions of CD28 mRNA,CD80 mRNA and CD86mRNA were decreased but the difference was not statistically significant(P>0.05). Conclusion The mRNA of CD28/B7 family costimulatory molecules is abnormally expressed in bone marrow mononuclear cells of IRP patients,and the down-regulation of CTLA-4 may play an important role in the development of IRP.

目的 探讨芳香烃受体AHR在溃疡性结肠炎(UC)组织中的表达及其对IL-17信号效应调控的作用.方法 10例UC患者和10例正常的结肠组织切片进行免疫组化检测,分析AHR在UC中的变化体外细胞实验研究AHR对IL-17诱导的炎症因子产生的调控作用.结果 AHR蛋白在UC肠上皮细胞中的表达量显著下降;体外细胞实验发现活化AHR或者过表达AHR均能抑制IL-17介导的趋化因子CCL20、CXCL2和细胞因子IL-6的产生;相反,在Hela细胞中干扰AHR可以促进炎症相关因子CCL20、CXCL2和IL-6的产生.结论 AHR能负向调控IL-17介导的炎症因子的产生,同时其表达量与UC的发生呈负相关,提示AHR有可能通过负相调控IL-17介导的炎症效应,抑制UC的发生,发展.

Objective] To investigate the effects of inhibiting pancreatic cancer of Cinobufotalin by bioluminescent imaging(BLI) in the BxPC3-luc2 cell xenograft nude mice.[Method] Twenty-four nude mice were subcutaneously injected BxPC3-luc2 pancreatic cancer cells. After seven days, tumor-bearing mice were divided into three groups:blank group, Cinobufotalin group(6g·kg-1, Cinobufotalin) and positive drug group(2mg·kg-1 DDP), eight mice for each group and continuous intraperitoneal injection of 14d. The tumor growth of pancreatic cancer in nude mice was detected by using the card scale and living creature luminescence imaging instrument at 7th and 14th days, and the animals were sacrificed at 14th day, and the solid tumor was weighed. [Result] Tumor bearing nude mice with injection of Cinobufotalin had no significant changes in body weight compared with the control group, while tumor weight and volume were significantly reduced; Tumor photon was also significantly reduced in nude mice treated with Cinobufotalin at after administration of 7th and 14th days, the inhibition rates of tumor photon were 30.11%and 63.81%respectively. But 6g·kg-1 Cinobufotalin was weaker than 2mg·kg-1 DDP. The effect of Cinobufotalin did not change significantly for body weight in mice, had certain advantages. [Conclusion] Cinobufotalin has a good inhibition of BxPC3 pancreatic cancer in nude mice.

目的 探讨养正消积胶囊对荷瘤裸鼠食管鳞癌的抑制作用及对其生存状态、白细胞计数及分类的影响.方法 食管鳞癌CaEs-17细胞株经培养并接种于裸鼠皮下,随机分组后给予低、中、高剂量的养正消积溶液灌胃,并与顺铂对照.观察荷瘤裸鼠生存状况,测量肿瘤抑制率,检测裸鼠白细胞计数及分类.结果 养正消积胶囊对荷瘤裸鼠食管癌组织具有抑制作用,对其日常生活质量、白细胞、淋巴细胞数量具有维持作用,与顺铂组比较,差异有统计学意义(P＜0.05).结论 养正消积胶囊能抑制荷瘤裸鼠食管癌组织增殖,有利于改善其生存状态与白细胞水平.

NLRCs inflammasome subfamily is an important member of the NLRs inflammasome family, which attracted more attention recently. NLRCs inflammasome subfamily members are mainly involved in the innate immune recognition of a variety of pathogenic microorganisms and resistance. Here, we mainly review the research progress of the NLRCs inflammasome subfamily members in recent years.

在慢性阻塞性肺病(COPD)的发病机制中炎症机制起重要作用,且有多种炎性细胞及细胞因子参与,其中核因子-κB(NF-κB)、血清白三烯(LT)B4、白介素(IL)-8可能作用谱更广、作用强度更大[1].我们观察老年COPD急性加重期和稳定期患者痰巨噬细胞中NF-κB和血清中LTB4、IL-8表达的变化,旨在了解在老年COPD发病中的炎性网络调节体系中三者之间的关系.

LH750全自动血液分析仪是Beckman-Coulter公司生产的新型全自动五分类血液分析仪,具有完善的计算分析系统.当血液样本中出现可疑的异常细胞时,仪器内设定的警示系统可自动报警,如异型淋巴细胞(Variant LY)、原始淋巴细胞(LY Blast)、原始单核细胞(Mo Blast)、原始中性粒细胞(NE Blast)、不成熟中性粒细胞1(Imm.NE1)(包括不成熟的中性粒细胞和/或中性杆状核粒细胞)、不成熟的中性粒细胞2(Imm.NE2)(包括早幼粒细胞、中幼粒细胞和晚幼粒细胞)及有核红细胞(NRBC)等信息.我们把这些可疑性报警提示设为中等(MED)敏感水平,研究此设定水平下报警提示功能的可靠性.