Objectives] To examine the expression of the Bombyx mori serine proteinase inhibitor 20 gene (BmSerpin-20) in Escherichia coli; and more specifically, to analyze its mRNA and protein expression levels in tissues, and its mRNA expression patterns in fat bodies after exogenous microorganism injection. [Methods] The BmSerpin-20 gene was inserted into the pET-28a vector. Recombinant plasmids were transfected into E. coli Transetta (DE3), and expression of the BmSerpin-20 protein induced with IPTG. The recombinant BmSerpin-20 protein was employed as an antigen to immunize New Zealand rabbits and to prepare a polyclonal antibody serum. The expression patterns of BmSerpin-20 were analyzed using real-time quantitative PCR and the Western blot technique. [Results] The BmSerpin-20 protein was successfully expressed in E. coli cells, purified and a polyclonal antibody against BmSerpin-20 prepared. The BmSerpin-20 gene and protein were expressed in the fat body, midgut, hemocyte, malpighian, integument, testis, ovary, with the highest expression in the integument. After treatment of B. mori larvae with nuclear polyhedrosis viruses, Micrococcus luteus, E. coli or Beauveria bassiana, the expression patterns of the BmSerpin-20 gene in the fat body was first down-regulated and then up-regulated. The expression level of BmSerpin-20 differed depending on the microorganism injected and injection time. [Conclusion] The expression pattern of BmSerpin-20 was successfully studied and may lay a foundation for further research on the function of this gene in the immune system of B. mori.

利用分布于油菜连锁群上的33对SSR核心引物,对12个育种品系和市场上的杂交油菜品种进行鉴定,探讨取样方案、检测方法和位点判读,将样品间SSR位点类型分为相同位点、疑似相同位点和差异位点3类.分析位点类型发现,12个油菜品种/系间的差异位点数为4 ～ 22,其中同父异母杂交种(3个)间差异位点数为4～16,同母异父杂交种(4个)间差异位点数为7 ～18,市场抽样的品种间差异位点数为12 ～ 20.同一品种不同样本间的差异位点数为0～2,据此,将区分品种间与品种内的差异位点阈值设定为2,即在利用33对引物对油菜杂交种与对照样品进行SSR分析时,当两样品间差异位点数＞2时,判定为不同品种;当差异位点数≤2时,判定为近似或极近似品种.盲样鉴定实验及其与田间鉴定方法的对比实验,均证明了本研究确定的SSR鉴定方法可行.

利用均匀分布于棉花26条染色体上的39对SSR核心引物对16个不同来源的棉花品种样品进行区别与鉴定实验,探讨了棉花品种鉴定的取样方案和位点异同判读方法.通过比较不同取样量样品包含的遗传信息,确定了基本的取样量,并将样品间位点异同类型分为相同位点、疑似相同位点和差异位点.据此对16个品种进行位点异同性分析,结果表明同一品种的不同样品间差异位点数均在0～2间,而不同品种间的差异位点数介于5～25.结合品种间差异位点的阈值分析,研究认为当两样品间差异位点数＞2时,即判定为不同品种;当差异位点数≤2时,即判定为近似或极近似品种.

In his masterpiece the Scarlet letter,Hawthorne tells us that all human beings are combination of goodness and evil.As human nature changes between goodness and evil,the science mastered by human beings may bring happiness or pains.