本研究对从广西5种不同动物体内分离到的禽Ⅰ型副粘病毒毒株,进行了HN基因的扩增、克隆和序列分析.5个APMV-Ⅰ分离株HN基因的核苷酸序列同源性为94.7％～98.4％,推导氨基酸序列同源性为96.3％～99％,同源性较高；5个毒株的HN基因与国内广东、山东、福建等地的当前流行株同源性较高,但与经典毒株同源性较低；遗传分析表明HN基因相对保守,都源于同一个基因亚群.

[Objective]This study was to determine molecular biology information of HA gene of H1N1 swine influenza virus isolated from Guangxi strains to provide references for monitoring swine flu in Guangxi. [Method]To analyze HA gene of H1N1 from Genbank, the primer was designed, and then HA genes of A / swine/Guangxi/1/2011 (H1N1) influenza virus were cloned, sequenced and compared via DNASTAR software. A phylogenetic tree was made using MEGA 4.0. [Result]The results indicated that the length of HA gene was 1701 bp and coded for 566 amino acids. Comparing with classical swine influenza H1N1, human -like H1N1 and Eurasian avian-like H1N1, the nucleotide homologies of HA genes were from 88.0% to 99.6%, from 76.3% to 77.3% and from 72.9% to 75.4%,respectively. Phylogenetic analysis revealed that the strain of A / swine/Guangxi/1/2011(H1N1) belonged to classical swine influenza H1N1 lineage and was similar to the strain A/California/04/2009 (H1N1), while it did not resemble human-like H1N1 and bird-like H1N1 at all. Amino acid sequence at the HA cleavage site was IPSIQSR↓G, which had characteristics of low-pathogenic influenza viruses. Among the eight glycosylation sites, six sites were located in HAl section and the rest in HA2. Amino acid in protein RBS of Guangxi strains possessed characteristics of both human and swine influenza virus. [Conclusion]This strain was pandemic(H1N1) 2009 virus and first isolated in Guangxi.

目的:分析社会经济因素和粮油因素对广西流行性乙型脑炎(乙脑)发病率的影响及其影响程度.方法:采用多元回归分析方法筛选相关因素.结果:将广西75个县作为整体,以 2006～2010年乙脑发病率作为应变量Y,以经济和粮油指标的16个因素为自变量进行多元逐步进入回归分析,先后入选的变量是第三产业收入(b3=0.001 1)、社会固定资产投资(b5=0.000 4)、农民人均纯收入(b6=0.016 4)、居民储蓄存款(b7=-0.001).结论:社会经济因素对乙脑发病率具有一定的影响,粮油产量对乙脑发病率没有影响.

We reported for the first time the isolation of H9N2 influenza virus from dogs in southern China. Genetic analysis of an isolate revealed that it was a novel genotype closely related to avian H9N2 virus. Epidemiologic studies demonstrated that the new H9N2-subtype virus was the causative agent of a disease in canine. Therefore, an appropriate countermeasure is urgently needed.

OBJECTIVE To understand the regulation of time distribution of epidemic encepbalitis B in Guangxi, and explore the characteristic of time distribution before and after immunization of the disease, which will provide scientific evidence for control and prevention countermeasures. METHODS Data on encephalitis B from 1960 to 2010 in Guangxi were analyzed with circular distribution method. RESULTS The average angle of JE incidence of three different stages were 188.592° , 181.880° and 180.961°, respectively; The average peak day of the disease were on the 10th July, the 3th July and the 2th July, respectively; The duration of the peak were from the 9th June to the 10th August, from the 1st June to the 5th August and from the 2nd June to the 1 st August, respectively. R value which indicated the morbidity of the disease gradually reduced from 0.869 to 0.845 before initial and routine immunization, in immunization management periods. The value indicating the mobidity of the disease dispersion was 29.60, which was the least value of s value, there was difference among the peak day at different stages (P﹤0.01). CONCLUSION There is a seasonal characteristic of the prevalence of the disease, the morbidity is gradually reduced, the peak day had been moved up and the peak stage is shortened along with the implementation of encepbalitis B immunization.

对广西猪源Ⅰ型副粘病毒F基因测序分析,为今后开展猪源副粘病毒的相关研究提供参考,也为有效防控副粘病毒感染奠定基础.参考GenBank发表的相关禽副粘病毒Ⅰ型(Avian paramyxovirus serotype Ⅰ,APMV-X)基因组序列,设计了1对特异性引物,用RT-PCR法分别扩增出病毒各F基因片段,并将目的基因片段回收纯化.测定得F基因的序列结果表明,从猪组织中分离获得1株猪源禽Ⅰ型副粘病毒,分离株F基因112～117位裂解位点的氨基酸组成为R-R-Q-R-R-F,与强毒株DQ417113、AF458012的裂解位点一致.同源性分析结果表明,分离株与标准强毒株的核苷酸同源性分别为87.5％和87.4％.猪源禽1型副粘病毒分离株为强毒株,属于基因Ⅰ型是传统型毒株,广西地区禽Ⅰ型副粘病毒宿主范围呈不断扩大趋势.

为了解禽Ⅰ型副黏病毒的跨种传播以及对HN基因进行序列分析,运用RT-PCR方法,扩增马源禽Ⅰ型副黏病毒广西分离株M10株的HN基因,将其克隆至PMD 18-T载体中,进行序列测定,序列分析表明,M10株的HN基因片段长度约为2.0kb,ORF为1716个碱基,编码571个氨基酸;ORF区与参考毒株的核苷酸同源性为80.9％-99.2％,推导的氨基酸同源性为88.6％-98.6％.在同源性比较的基础上,进一步绘制禽Ⅰ型副粘病毒HN基因的系统进化树,分析表明,M10株与参考毒株YG03、NDV-03、FP1等亲缘关系较近.

【Objective】This study was aimed to understand the biological characteristics of horse origin avian paramyxovirus serotype I and provide references for preventing and controlling the paramyxovirus interspecies transmission.【Method】 In Baise of Guangxi,causative agents were isolated from suspicious samples of horse using chicken embryos inoculation and cultured.After serological experiment and pathogenicity identification,F gene of isolates was amplified using RT-PCR and sequenced.【Result】The isolated strain could agglutinate chicken erythrocytes and was inhibited by Newcastle disease virus antiserum.Its titer of HA and HI was 27and 210,respectively.The MDT and ICPI of chicken embryos was 72 h and 1.48,respectively.The isolated strains,which was moderate susceptible,belonged to genotype Ⅶ,sharing 95.9% and 95.1% nucleotide sequence homologies with NDV04-21 and TW-96p,respectively.According to the phylogenetic tree of F gene,the isolates,NDV04-21 and TW-96p belonged to the same branch,which were APMV-I genotype Ⅶ.Amino acid sequence of the isolate fusion(F) protein cleavage site 112-117 was 3'-R-R-Q-K-R-F-5',which was in accordance with standard virulent strain HER33 and F48E9.【Conclusion】The results indicated that APMV-I infected horses.It was reconfirmed that hosts of APMW-1 were developing in Guangxi area.