目的:分析全子宫切除术后阴道断端裂开的病例特点,识别高危因素、探索治疗方法.方法:回顾分析北京大学第三医院妇产科2009年至2021年行全子宫切除或广泛/次广泛子宫切除术后发生阴道断端裂开的18例患者的临床资料,对其术前临床特征、手术参数、术后发病时间、诱发因素、症状、脱出脏器特征及修复方式进行描述.结果:阴道断端裂开发病率为0.2％(18/8929),患者平均年龄(47.1±7.5)岁,平均体质量指数(BMI)(21.4±2.0)kg/m2.前次手术为腹腔镜手术15例、开腹手术3例.手术指征为恶性肿瘤15例、癌前病变1例、良性疾病2例.中位发病时间128.5 d.发病诱因包括性生活8例、经阴道超声检查3例、排便困难3例、无明显诱因4例.经阴式修复11例、腹腔镜修复5例、开腹1例、腹腔镜+阴式1例.4例患者同时行部分大网膜切除术.结论:全子宫切除术后阴道断端裂开多发生在恶性肿瘤患者术后,主要诱发因素为性生活及经阴道超声检查,多数可经阴式修复.

Background Retroperitoneal fibrosis is a rare disease characterized by chronic nonspecific inflammation, which leads to clinical compression manifestations of retroperitoneal organs especially ureter. Approximately 70 percent of retroperitoneal fibrosis cases are idiopathic which has no clear etiology. This study reported a rare case of a 48-year-old woman presented with idiopathic retroperitoneal fibrosis and endometrial cancer. Case presentation A 48-year-old woman presented with irregular vaginal bleeding without abdominal pain, bloating or discomfort. The patient was diagnosed iRPF after splenectomy 13 years ago. Then she took prednisone for 2 years and took tamoxifen for about 11 years. She stopped taking the medication from October 2019 to May 2020 and then started taking tamoxifen again until November 2020. Two weeks after she stopped taking tamoxifen, she presented with irregular vaginal bleeding. Gynecological ultrasound revealed a thick endometrium with uneven echo enhancement and blood flow signals. Then diagnostic curettage was performed with pathological examination showed endometroid carcinoma. Later, the patient was admitted to Peking University Third Hospital for surgery. Preoperative imaging examinations, including CT, MRI, and PET/CT, all showed pelvic enlarged lymph nodes and they were highly suspected to have lymph node metastasis. The patient underwent laparoscopic surgical staging and enlarged lymph nodes in the pelvic and aortic regions were removed. Finally, the pathology confirmed that endometrioid adenocarcinoma and fibrosis, but there was no tumor infiltration in these enlarged lymph nodes. The patient is now in good condition. Conclusion This case report stressed the difficulty to distinguish between lymph node metastasis and inflammatory hyperplasia by common imaging methods. Due to increased surgical difficulty among retroperitoneal patients, lymphadenectomy should be carefully evaluated to avoid additional surgical complications and over-treatment.

盆腔器官脱垂严重影响患者生活质量,对于高龄、合并症多的中重度盆腔器官脱垂的患者,如无性生活要求,可考虑行阴道封闭术.标准的阴道部分封闭术( Le Fort colpocleisis,LFC)是Le Fort 1877年首次提出的[ 1 ].LFC 术后子宫发生恶性肿瘤较少见,2019年12月我科收治1例LFC术后发生子宫癌肉瘤,报道如下.

目的 探讨妊娠中期卵巢良性肿瘤患者应用腹腔镜手术治疗的有效性和安全性.方法 回顾性分析2015年1月至2018年6月期间,北京大学第三医院收治的18例因中期妊娠合并卵巢良性肿瘤入院行腹腔镜手术治疗的患者临床资料,包括患者年龄、手术孕周、手术时间、术中出血量、术后住院日、术后病理结果、分娩结局.结果 18例患者手术均为腹腔镜,无中转开腹,无手术及麻醉并发症,患者平均年龄(30.5±3.0)岁,孕周(16.0±5.0)周,手术时间(61.0±19.0)min,术中出血(8.4±5.0)mL,术后住院时间(1.8±1.0)d,最终18例足月分娩,其中剖宫产7例,阴道分娩11例,新生儿均无窒息、畸形发生.结论 腹腔镜手术治疗中期妊娠合并卵巢良性肿瘤,安全有效,效果显著,对妊娠结局及新生儿无不良影响,可作为常规技术进行开展.

目的 探讨女性术后慢性盆腔痛的病因、诊断流程及治疗方法.方法 回顾性分析2019年7月 ～2020年6月我院诊治的60例术后慢性盆腔痛资料.根据病因进行个体化治疗,包括加巴喷丁、神经阻滞、抗生素、口服避孕药、手术、中成药等.疗效评价标准:①完全缓解,疼痛消失,视觉模拟评分(Visual Analogue Scale,VAS)降至0分;②部分缓解,疼痛减轻,VAS评分或疼痛频率降低≥50％;③无效,VAS评分及疼痛频率降低<50％.结果 诊断神经病理性疼痛34例(56.7％),盆腔炎症性疾病11例(18.3％),子宫内膜异位症7例(11.7％),盆腔炎症性疾病合并神经病理性疼痛2例(3.3％),盆腔炎症性疾病合并子宫内膜异位症2例(3.3％),子宫内膜异位症合并神经病理性疼痛2例(3.3％),肠易激综合征1例(1.7％),原因不明1例(1.7％).除5例因备孕未治疗外,55例治疗后随访1～9个月,平均2.6月.完全缓解18例,部分缓解34例,无效3例.有效率94.5％(52/55).结论 手术相关女性慢性盆腔痛可能的病因包括神经病理性疼痛、子宫内膜异位症、盆腔炎症性疾病,亦可能为消化系统或泌尿系统疾病,诊断过程中应仔细鉴别,针对病因进行个体化治疗.

目的 探讨晚期上皮性卵巢癌腹腔镜初次肿瘤细胞减灭术的可行性和安全性.方法 回顾性分析2010年1月～2019年12月肿瘤直径≤10 cm的国际妇产科联盟(International Federation of Gynecology and Obstetrics,FIGO)手术病理分期为Ⅲa～Ⅳ期上皮性卵巢癌126例资料,行腹腔镜手术50例,开腹手术76例.2组年龄、BMI、肿瘤直径、手术病理分期、病理类型和病理分级差异均无统计学意义(P>0.05).比较2组手术参数及随访结果.结果 与开腹组相比,腹腔镜组手术时间短[(344.6±125.9)min vs.(443.1±124.2)min,t=-4.335,P=0.000],出血少[中位数200(20～2000)ml vs.600(50～3500)ml,Z=-6.241,P=0.000],术后排气早[2(1～7)d vs.4(1～13)d,Z=-5.380,P=0.000],开始化疗早[11.5(3～39)d vs.15.5(8～65)d,Z=-2.724,P=0.006],但肿瘤细胞减灭术满意率低[32.0％(26/50)vs.64.5％(49/76),χ2=12.734,P=0.000].2组并发症发生率差异无统计学意义(P=0.960).腹腔镜组和开腹组无进展生存期(中位数35月vs.27月,log-rankχ2=0.861,P=0.353)和总生存率(3年生存率86.6％vs.77.6％,5年生存率70.0％vs.58.9％,log-rankχ2=1.381,P=0.240)差异均无统计学意义.结论 经过严格选择的晚期上皮性卵巢癌行腹腔镜初次肿瘤细胞减灭术是可行的,其预后并不逊于开腹手术.

目的 了解子宫颈癌根治性子宫切除术后患者下尿路症状患病情况及其相关因素.方法 选择2012年1月至2015年3月在全国13家研究中心接受Piver Ⅲ型子宫切除的690例宫颈癌患者,并按照年龄和体质指数匹配妇科门诊无手术史良性疾病患者690例作为对照组,采用女性下尿路症状国际尿失禁标准问卷(ICIQ-FLUTS)和膀胱过度活动症评分(overactive bladder symptom score,OABSS)对两组患者进行问卷调查.结果 宫颈癌组下尿路症状患病率(78.3％,540/690)与对照组(78.7％,543/690)相似(P＞0.05).宫颈癌组储尿期症状患病率(66.2％,457/690)低于对照组(75.5％,521/690)(P＜0.05),而排尿期症状(52.3％,361/690)和膀胱过度活动症(overactive bladder symptom,OAB)(14.5％,100/690)患病率显著高于对照组(24.1％,166/690;8.8％,61/690)(P＜0.05).单因素分析显示,腹腔镜手术、术中切除宫旁长度或阴道长度＞3 cm与排尿期症状、OAB患病相关(P＜0.05).Logistic分析显示,腹腔镜手术是排尿期症状(OR=2.380,95％CI:1.664～3.405)和OAB的危险因素(OR=1.972,95％CI:1.155～3.367).结论 宫颈癌患者根治性子宫切除术后排尿期症状及OAB患病率升高,可能与切除较多的宫旁和阴道组织有关.

目的 探讨孕前宫颈环扎术治疗宫颈机能不全的疗效.方法 回顾分析我科2017年1月～2020年6月20例宫颈机能不全孕前行腹腔镜或经阴道宫颈环扎的临床资料.16例行腹腔镜宫颈环扎术,利用简易举宫器,于子宫峡部外1/3浆肌层由后向前或由前向后进针环扎宫颈内口;4例行经阴道宫颈环扎术,上推膀胱后打开后穹隆阴道黏膜至宫颈筋膜,向上分离至宫颈内口上1 cm,于宫颈内口水平缝合宫颈1/2肌层1周.结果 20例手术顺利,手术时间(40.9±12.1)min,术中出血量(7.8±4.3)ml.腹腔镜宫颈环扎术16例术中出血(7.9±4.7)ml;12例术后妊娠,其中9例已分娩(7例足月分娩).经阴道宫颈环扎术4例术中出血(7.5±2.9)ml;2例术后妊娠,其中1例足月顺产,1例目前孕30周.7例有中孕期宫颈环扎失败史中,5例术后妊娠,其中3例已分娩,分娩孕周分别为35、36+5、38+1孕周,1例尚在孕期,1例早孕期胚胎停育.结论 对于宫颈机能不全的患者,尤其是经历孕期宫颈环扎术后失败的患者,孕前行宫颈环扎术是一种简单、安全、有效的方法.

Introduction and Hypothesis To evaluate the pelvic floor muscle function (PFMF) of cervical cancer patients after type QM-C hysterectomy and to explore the relationship between decreased PFMF and related factors.. Methods This was a multi-centered retrospective cohort study. 181 cervical cancer patients underwent type QM-C hysterectomy were enrolled from 9 tertiary hospitals. Strength of PFMF were measured by using neuromuscular apparatus (Phenix U8, French). Risk factors contributed to decreased PFMF was analyzed by univariate and multivariate ordinal ploytomous logistic regression. Results Totally 181 patients were investigated in this study.0-3 level of type I muscle fibre strength(MFSI) was 52.6%(95/181),0-3 level of type ⅡA muscle fibre strength(MFSⅡA) was 50%(91/181). Subjective stress urinary incontinence was 46%(84/181),urinary retention was 27.3%(50/181),dyschezia was 41.5%(75/181),fecal incontinence was 9%(18/181).①MFSI：Multivariate ordinal ploytomous logistic regression shows that the follow-up time(p<0.05),chemotherapy and radiotherapy (p=0.038) are independent risk factors of MFSI’s reduction after type QM-C hysterectomy.② MFSⅡA：Multivariate ordinal ploytomous logistic regression shows that the follow-up time(p<0.05) are independent risk factors of MFSⅡA’s reduction after type QM-C hysterectomy. The pelvic floor muscle strength(PFMS) increased after 9 months than in 9 months after operation, which showed that the PFMS could be recovered after operation. Conclusions We advocate for more attention and emphasis on the PFMF of Chinese female patients with cervical cancer postoperation. Contribution of the Paper The key messages of the article is that PFMF after QM-C hysterectomy have not been analyzed by current study.The new knowledge added by this study is that 3 months after radical hysterectomy patients’ should do pelvic floor rehabilitation exercises.

目的 探讨单孔腹腔镜在妇科常见手术中的应用价值.方法 选取2016年4月—2018年8月在本院实施的单孔腹腔镜手术66例作为单孔腹腔镜组,其中输卵管切除27例,附件囊肿剔除术25例,全子宫附件切除14例;选取相同疾病的传统腹腔镜手术66例作为传统腹腔镜组,比较手术时间、切口开关时间、手术操作时间、术中出血量、术后疼痛评分(采用视觉模拟评分法,简称VAS评分)、术后住院时间、患者满意度情况.结果 两组均成功实施手术,无手术并发症发生.单孔腹腔镜组附件手术总时间(37.5±11.2)min、切口开关时间(8.4±2.6)min、手术操作时间(27.4±10.4)min,均明显长于传统腹腔镜组(20.1±6.4)min、(3.5±0.4)min、(18.1±7.9)min,差异有统计学意义(P<0.001);单孔腹腔镜组术中出血量(10.2±3.9)ml、术后疼痛VAS评分(4.4±1.3)分均高于传统腹腔镜组(3.5±1.6)ml、(2.7±0.8)分,差异有统计学意义(P<0.001).单孔腹腔镜组子宫切除手术总时间(62.1±22.1)min、切口开关时间(7.9±2.9)min、手术操作时间(45.5±19.3)min,均明显长于传统腹腔镜组(41.2±12.4)min、(3.4±0.7)min、(32.1±9.9)min,差异有统计学意义(P<0.05);单孔腹腔镜组术中出血量(25.7±12.6)ml、术后疼痛VAS评分(5.1±1.6)分高于传统腹腔镜组(12.4±3.8)ml、(3.4±1.4)分,差异有统计学意义(P<0.05).单孔腹腔镜组对手术的主观评价明显优于传统腹腔镜组,非常满意率分别为72.7％(48/66)和59.1％(39/66).结论 单孔腹腔镜行妇科常见手术,手术时间、操作难度增加,但切口美观隐匿,患者治疗满意度增加,在妇科疾病的治疗中有一定的应用价值.

目的:探讨应用分碎器的腹腔镜子宫肌瘤手术后继发医源性寄生性平滑肌瘤的临床特点.方法 :回顾性分析2006 ～2019年北京大学第三医院收治的7例寄生性平滑肌瘤患者的临床资料.结果 :7例患者均有应用分碎器的腹腔镜子宫肌瘤手术史.发病年龄29 ～47岁,中位年龄38岁.2例表现为月经增多;1例自行触及下腹部包块;另4例无任何症状.本次发病距前次手术间隔4～8年,中位时间7年.寄生性平滑肌瘤均为多发,位于盆腹腔腹膜、肠系膜、大网膜、卵巢、阑尾、腹壁瘢痕,直径0.5 ～ 12 cm.切除后病理检查均为良性平滑肌瘤,伴有玻璃样变(2例)、梗死(2例)或黏液变性(2例).术后随访9 ～161个月,中位时间15个月,1例复发.结论 :寄生性平滑肌瘤可继发于应用分碎器的腹腔镜子宫肌瘤术后,经个体化的手术治疗预后良好.

目的 探讨单一部位切口腹腔镜手术(laparoendoscopic single-site surgery,LESS)在巨大卵巢肿瘤(直径>10 cm)中的应用价值.方法 对2017年4月～2018年12月在我院因巨大卵巢囊肿行卵巢囊肿剔除或患侧附件切除的46例资料进行回顾性分析,其中LESS 23例,肿瘤直径(16.32±5.06)cm,传统三孔腹腔镜手术23例,肿瘤直径(14.08±2.78)cm,2组差异无显著性(t=1.861,P=0.069).比较2组手术时间、术中出血量、手术并发症、术后24 h疼痛视觉模拟评分(Visual Analogue Scale,VAS)、住院时间、术后满意度.结果 LESS组行卵巢囊肿剔除18例,患侧附件切除2例,卵巢肿瘤分期手术(大网膜部分切除+盆腔及腹主动脉旁淋巴结活检)3例;三孔组分别为16例、6例、1例.2组手术过程顺利,均无术中及术后并发症.LESS组无囊肿破裂,三孔组囊肿破裂5例(21.7％),差异有显著性(P=0.049),2组术式、手术时间、术中出血量、术后住院时间差异无显著性(P>0.05),但术后切口疼痛VAS评分LESS组[(4.17±1.24)分]高于三孔组[(3.47±0.94分)](t=2.157,P=0.036).术后6个月2组满意度差异无显著性(P>0.05).结论 对于巨大卵巢肿瘤,LESS手术难度低,探查全面,安全可行,患者满意度高,具有一定的应用价值.

压力性尿失禁(stress urinary incontinence,SUI)是指喷嚏、咳嗽、大笑或运动时腹压增高后出现不自主的尿液自尿道口漏出.SUI限制了女性日常活动及运动,严重影响其生活质量,并导致巨大心理压力.SUI的危险因素有:年龄、遗传 因素、雌激素水平降低、便秘、慢性咳嗽及肥胖等[1],其中肥胖是SUI的独立危险因素[2].

子宫腺肌病是妇科临床常见病症,较大的囊性病变罕见,正确认识该病临床特点和鉴别诊断对临床治疗有重要意义,手术病灶去除是子宫囊性腺肌瘤的一种可行且有效的治疗手段.本文就北京大学第三医院妇科诊断并治疗的1例子宫囊性腺肌瘤的临床病例资料进行分析.

Objectives: To assess the feasibility and outcome of primary laparoscopic cytoreductive surgery on advanced epithelial ovarian cancer in comparison with conventional open surgery. Materials and Methods: Patients undergoing primary laparoscopic cytoreductive surgery (LCS) from March 2007 to December 2016 were matched to controls treated with laparotomic cytoreduction during the same period. Procedural data and outcomes were analyzed. Results: The LCS group (n = 64) and laparotomic group (n = 68) had similar age, BMI, stages, histologic type and grading. The LCS group exhibited significantly less operating time (P < 0.001), less intraoperative blood loss (P < 0.001), and shorter time to recover postoperatively (P = 0.002). No statistical difference was observed for the number of pelvic and para-aortic lymph nodes dissected (P = 0.326 and P = 0.151). Significant difference was observed in satisfaction of the cytoreduction (95.3% vs. 76.5%, P = 0.008). No significant difference were observed either in intra-operative or in post-operative complications between the two groups (P = 0.250). Three patients in the LCS group experienced intra-operative complications (4.7%) and were all treated laparoscopically. The conversion rate was 3.1%. No significant differences were observed in the progression-free survival and overall survival between the two groups during the medium follow-up of 18 months (P = 0.236 and P = 0.216). The 2-year and 3-year progression-free survival was 67.9%, 55.5% in LCS group and 53.8%, 33.3% respectively in the control group. The 2-year and 3-year overall survival was 95.8%, 88.7% respectively in the LCS group and 89.0%, 83.7% in the control group. Conclusions: Primary laparoscopic cytoreductive surgery in some strictly selected advanced stages of EOC patients was feasible and safe, resulting in oncologic outcomes not inferior to those in open surgery.

Class III radical hysterectomy (RH III)_plus pelvic lymphadenectomy is the standard surgery for early stage cervical cancer (CC) patients, the 5 year survival rate is about 90%, but pelvic floor disorders especially bladder dysfunction are common due to damaged vessels and nerve fibers following surgery. Transcutaneous electrical stimulation (TENS) treatment has been used to treat bladder disorders for many years, but its effect on cervical cancer patients, the best treatment time point and stimulated protocol, had never been assessed. The aim of this study is to investigate the efficacy of TENS treatment on lower urinary tract symptoms (LUTS) after RH III in CC patients.

Purpose To evaluate parameter changes of pelvic floor muscles in patients with female pelvic organ prolapse (POP) through diffusion tensor imaging (DTI).Materials and Methods Fifty female patients with POP at Ⅲ or ⅣV degree (research group) and fifty healthy women volunteers (control group) were collected to make prospective research.The axial DTI sequence was performed in subjects,with b=500 s/mm2,at 15 directions.DTI original images were imported into the post-processing software,and the corresponding DTI mappings were formed.In the original images,pelvic floor muscles were sketched out layer by layer,and various DTI parameters,including fractional anisotrophy (FA),mean diffusivity (MD),λl,λ2,λ3,linear anisotropy (CL),planar anisotropy (CP) and spherical anisotropy (CS),were calculated.Six kinds of pelvic floor muscles,including anal sphincter,superficial transverse perineal muscle,pubic visceral muscle,puborectal muscle and iliococcygeal muscle were measured,and five of them were measured on both sides.The difference of DTI parameters between right and left side of each muscle,and the difference of DTI parameters between research and control group were compared.Results Between the right and left side of five kinds of pelvic floor muscles in control group,significant difference was only found in FA,λ3,CP,CS value of internal obturator muscle (P＜0.01,P＜0.001).There were significant differences in FA,λ3,CL,CP and CS values of the left and right muscles in the study group (P＜0.05,P＜0.001).Compared with control group,FA value and CS value of superficial transverse perineal muscle,CL value of left pubic visceral muscle,FA,λ3,CL,CS and CP value of right pubic visceral muscle,λ1 and λ3 value of left iliococcygeal muscle of research group were significantly different (P＜0.01,P＜0.05).Conclusion DTI for observation of female pelvic floor muscle is technically feasible,and it can detect the injury of pelvic floor muscle in POP patients,and may be helpful to reveal the pathogenesis of POP.

Objective To investigate the value of laparoscopic bilateral uterine artery occlusion combined with hysteroscopic resection in the treatment of cesarean scar pregnancy ( CSP ) . Methods A total of 62 patients with CSP were admitted to this hospital from January 2013 to May 2015.Transvaginal ultrasound was performed for primary screening , and pelvic MRI for diagnosis. Uterine arteries were separated and coagulated during laparoscopic surgery , followed by hysteroscopic surgery .The postoperative serum hCG level tests and ultrasonography examinations were conducted for evaluating the surgical outcomes . Results The procedure was successfully completed under laparoscopy and hysteroscopy without surgical complications or conversions to open surgery in all the cases.The median blood loss was 30 ml (P25:20 ml, P75:100 ml).In 4 patients the intraoperative bleeding reached 600, 600, 800, and 800 ml, respectively, due to large lesions, with blood transfusions requirement in 3 patients.One patient received secondary hysteroscopy due to unsatisfied hCG decrease .The serum hCG levels dropped to normal in 4-75 d (30.9 ±16.9 d) after operation. The menstuation cycles recovered to normal in 20-75 d (34.8 ±11.4 d).During follow-ups for 4-32 months (15.8 ±8.0 months) in 62 cases, 5 patients had subsequent pregnancy , 4 of which had full-term pregnancy and 1 of which experienced CSP again and received hysteroscopic removal of pregnancy . Conclusion Laparoscopic bilateral uterine artery coagulation and hysteroscopic removal of pregnancy is an ideal choice for the treatment of CSP with good outcomes .

Objective: To evaluate the quality of observational studies on pelvic organ prolapse in China. Methods: The checklist of strengthening the reporting of observational studies in epidemiology (STROBE) statement was applied to evaluate the observational studies. The articles were searched in the SinoMed database using the terms: prolapse, uterine prolapse, cystocele, rectal prolapse and pelvic floor; limited to Chinese core journals in obstetrics and gynecology from January 1996 to December 2015. With two 10-year groups (1996-2005 and 2006-2015), the χ(2) test was used to evaluate inter-group differences. Results: (1) A total of 386 observational studies were selected, including 15.5%(60/386) of case-control studies, 80.6%(311/386) of cohort studies and 3.9% (15/386) of cross-sectional studies. (2) There were totally 22 items including 34 sub-items in the checklist. There were 17 sub-items (50.0%, 17/34) had a reporting ratio less than 50% in all of aticles, including: 1a (study's design) 3.9% (15/386), 6a (participants) 24.6% (95/386), 6b (matched studies) 0 (0/386), 9 (bias) 8.3% (32/386), 10 (study size) 3.9%, 11 (quantitative variables) 41.2% (159/386), 12b-12e (statistical methods in detail) 0-2.6% (10/386), 13a (numbers of individuals at each stage of study) 18.9% (73/386), 13b (reasons for non-participation at each stage) 18.9%, 13c (flow diagram) 0, 16b and 16c (results of category boundaries and relative risk) 9.6% (37/386) and 0, 19 (limitations) 31.6% (122/386), 22 (funding) 20.5% (79/386). (3) The quality of articles published in the two decades (1996-2005 and 2006-2015) were compared, and 38.2%(13/34) of sub-items had been significantly improved in the second 10-year (all P<0.05). The improved items were as follows: 1b (integrity of abstract), 2 (background/rationale), 6a (participants), 7 (variables), 8 (data sources/measurement), 9 (bias), 11 (quantitative variables), 12a (statistical methods), 17 (other analyses), 18 (key results), 19 (limitations), 21 (generalisability), 22 (funding). Conclusions: The quality of observational studies on POP in China is suboptimal in half of evaluation items. However, the quality of articles published in the second 10-year have significantly improved.

Objective. Epithelial ovarian cancer (EOC) is the deadliest type of ovarian cancer, but the mechanisms contributing to its tumorigenesis are not well understood. Herein, we will elucidate the role of Ten-eleven translocation 1 (TET1) in EOC development Methods. The expression of TETI in EOC cell lines and primary samples was examined by western blot and immunohistochemistry. The biological role of ectopic TETI overexpression was revealed by a series of in vitro functional studies. Its downstream signaling pathway was predicted by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of microarray data. The methylation level and expression of Wnt/beta-catenin signaling inhibitors Dikkopf 1 (DKK1) and secreted Fzd receptor protein 2 (SFRP2) were examined by Chromatin immunoprecipitation (ChIP) assay, Epimark (TM) 5hmC and 5mC level analysis and quantitative RT-PCR Small interference RNA (siRNA) technology was used to investigate the biological roles of DKK1 and SFRP2. Results. TETI expression was inversely correlated with clinical stage in patients with EOC by tissue microarray (TMA).TET1 expression was undetected in 6 types of EOC cell lines. Ectopic expression of TETI inhibited colony formation, cell migration and invasion in SKOV3 and OVCAR3 cells. Furthermore, TM overexpression reversed the epithelial-mesenchymal transition (EMT) process of SKOV3 cells. Mechanistically, TETI potently inhibited canonical Wnt/beta-catenin signaling by demethylating and upregulating two upstream antagonists of this pathway, SFRP2 and DKK1, which was associated with inhibition of EMT and cancer cell metastasis. Conclusion. This study uncovers that TETI has potent tumor-suppressive effects in EOC by activating Wnt/beta-catenin signaling inhibitors Dial and SFRP2. (C) 2017 Published by Elsevier Inc.