本实验首先双酶切含有Hind3和NcoI酶切位点的PRRSV目的基因的质粒pUC-57—PRRSV N gene，利用酶切的目的基因与表达载体pET-28a，,构建重组表达载体pET-28a- PRRSV N gene，为实现目的蛋白原核表达，建立针对该病的快速、有效的检测方法研发安全高效的疫苗等针对猪群PRRSV进行后续研究的奠定基础和前提条件。

在雾霾天气越来越严重、环境污染日益恶化的背景下,环境的污染问题明显突出,也成为经济发展的一大障碍.为此,绿色经济发展得到更多人的认识和赞同.人们在发展经济的同时,重视绿色经济的发展,同时更重视环境科技创新在绿色经济发展中的应用.绿色文明是追求人类和自然界的和谐统一,环境保护与经济发展的协调发展.它需要对绿色资源的可持续利用,资源利用不能超过替代资源的发展速度;污染排放量不能超过自净系统的自然规律,科学合理地利用资源.科技创新、绿色文明符合中国当前可持续发展的总体要求,是发展绿色循环经济的思想基础.

To study the influences of various environmental factors on the biofilm formation of Campylobacter jejuni,the orthogonal tests were used to synthetically analyze the influences of the air condition,type of medium and temperature on the biofilm formation with the optical microscopy and scanning electron microscopy.The results showed that the influences of three factors in descending order were the air condition,temperature,and type of medium.The biofilm-forming capacities were also different with each of these factors at different levels.The results indicate that the appropriate aerobic and oligotrophic environments were more conducive to the biofilm formation.

大学生是文化消费的特殊群体,文化消费的内容、质量等都对大学生与社会的发展起着重要的影响。目前关于当代大学生文化消费的研究却还存在问题,通过对国内与国外,关于此专题的研究进行归纳和分析,发现其中不足之处,为新的研究方向提供意见。

伴随着欧债危机的不断恶化,国际评级机构标普不断下调欧洲多数国家主权评级,使得欧洲经济未来走势难以预料;作为世界第一大经济体的美国,目前似乎只是以不断的量化宽松政策来刺激经济的发展,虽然缓和了美国经济体当前的一些处境,却让世界多数国家无辜为美元的贬值不断买单;中东石油区以及伊斯兰地区的持续动乱,更是在一定程度上为世界经济的复杂走势推波助澜,种种不稳定因素直接导致了国际货币市场走势愈加扑朔迷离。与此同时,一些国外政客为缓解民众注意以及一些经济组织不可告人的考量,不断对中国汇率施压,人民币在国际外汇市场不断升值,使得进出口企业财务汇兑损益风险在当前的外汇市场上逐渐凸显,多数外向型的企业的财务资金安全受到直接冲击,由此带来的汇率市场瞬息万变也让很多进出口企业措手不及,企业的长久稳定发展带来难以估计的风险隐患。如何能有效地尽可能地规避外汇汇率冲击,采用有效的手段缓释汇兑损益带来的财务风险,成为外向型企业财务人员亟待解决的课题。

<正>近年研究发现,硒具有促进和调节人体免疫、抗肿瘤、抗氧化等许多重要的生理功能。我国70%的地区属于缺硒或低硒地区[1],因此日常补硒就成为保证机体硒需要量的重要途径。但是在临床上补硒的量并没有确定一个适当的范围,结果造成一部分人盲目补硒,使硒的摄入量过大或摄入时间过长,导致硒中毒时有发生。正常机体中各T淋巴细胞亚群相

<正>在拉深加工过程中,对遇到的问题应仔细观察,细心分析,从拉深加工工艺、拉深模各零部件结构、拉深材料等众多的影响因素中找出具体的原因,并采取正确的处理措施。1.制品形状与尺寸发生变化(1)一面侧壁低而厚,一面侧壁高而薄。原因分析:压边圈不平整,板料在拉深过程中进入凹模的阻力不均匀,致使变形阻力小的那一面侧壁低而厚,阻力大的那一面侧壁高而薄,如图1所示。

本文分析了金属粉末注射成形工艺过程(金属粉末注射成形坯、注射成形坯的脱黏、烧结)产生的质量缺陷、采取的对策及该成形技术在汽车工业中的应用前景.

The US10 gene was expressed in pET-32a(+)/Rosetta(DE3) pLys system using the DNA of Duck enteritis virus(DEV) C-KCE as temple,US10 gene ORF was amplified by polymerase chain reaction(PCR).Then the recombinant protein of US10 gene induction with IPTG by SDS-PAGE analysis showed that the object protein is mainly expressed as inclusion bodies.Using the Ni-NTA Purification System,the recombinant protein immunized to rabbits was purified to make the antiserum.The titer of the antiserum tested by agar diffusion reaction was 1∶4.The titer of the antiserum tested by ELISA was 1∶102 400.The prepared antiserum has a high specificity with the western-blot analysis,and the indirect immunofluorescence(IIF) test proved that the antiserum could react with DEV US10 encoded product,which lays a foundation for further research on molecular structure and function.

With the continuous development of PLC technology,the programmable controller PLC and transducer combination should be used to hoist con-trol system of mine hoist,the control performance and the level of automation,safety,reliability have reached a new height,has overcome the traditional mine hoist control system in the presence of poor reliability,complex operation,high failure rate,the waste of energy,low efficiency and safety protec-tion,atresia,monitoring system is not perfect and other shortcomings,greatly enhances the mine safety and economic efficiency.

To investigate the effect of sodium selenite poisoning on the immune functions in mice.The experimental animals were divided into control group(0.00 mg/kg·d),low dose(0.14 mg/kg·d),middle dose group(0.54 mg/kg·d) and high dose(2.16 mg/kg·d).Sodium selenite groups mice were killed after continuous intragastric feeding for 30 days.Phagocytosis of chicken red blood cells by macrophage and hemolytic plaque assay were used to examine the phagocytic function of mouse peritoneal macrophages and numbers of antibody producing cells.Sodium selenite poisoning significantly resuced the phagocytic function of mouse peritoneal macrophages and the number of antibody producing cells.It has dose-dependent correlation.Sodium selenite poisoning inhibits the function of nonspecific immunity in mice,which presents the apparent dose effect relationship.

Single-factor experiment was designed,and 160 natal Jilin white geese and Nongan geese of the similar body weight were chosen as experimental animal. The geese of either variety were then divided into 4 treatment groups randomly and NRC-based dietary was fed to them according to their two developmental stages,0 to 4 weeks old,and 5 to 8 weeks old.By slaughter experiments and RT-PCR(reverse transcription polymerase chain reaction) technique,the expressions of MC4R(melanocortin receptor-4)and its receptor in liver,breast and leg muscles during different developmental stages were detected to further study the influence of different genetic basis on levels of MC4R mRNA and its receptor.The results show that the meat performance of different genetic basis and the expression level of MC4R mRNA in muscle show a correlation of some extent.The expression level of MC4R mRNA in leg or in breast muscle of Jilin white geese is higher than that in Nongan geese.Live weight,leg muscle weight,breast muscle weight,daily gain weight of Jilin white geese are higher than those of Nongan geese.

以生态文明建设引领产业结构调整,形成促进生态文明建设的产业结构,其本质是以科学发展观为指导,逐步形成可持续的产业发展模式.淄博市的探索和实践,为生态文明建设背景下老工业城市结构调整提供了一个可资借鉴的范例.淄博市四个阶段的结构调整重点都始终围绕着“结构调整是主线、节能降耗是关键、环境保护是命门”的工作思路.

为了研究更快、更加准确分离空肠弯曲杆菌的方法,试验同时采用常规生化法对由12份猪盲肠内容物中分离的可疑菌进行检测,得到了6个阳性样品,经特异性PCR检测,2个样品可定性为空肠弯曲杆菌,检测时间为7 d;而对所得菌株直接进行PCR检测,检测结果相同,检测时间为2 d。结果表明:PCR可以快速、特异性地检测空肠弯曲杆菌,与常规生化检测法相比是一种更有效的方法。

The environmental quality of Zibo city is easily influenced by external factors.The condition of large pollutant emission amount and high pollutant emission intensity exist because of unreasonable industrial structures,which caused the accumulation of pollution.In recent years,Zibo city has given full play to the reversal effect of environmental protection,by the way of establishing and perfecting environmental policy and law system,strictly executing higher standards than the national and provincial standard,greatly developing high-tech industry and modern service industry,speeding up the transformation of traditional industries,and has made great efforts in promoting the upgrading of industrial structure and the transformation of economical development mode.

The main pollutants in zibo city environment air SO2 and PM10 at different height of chroma value analysis,find out the vertical distribution law;And by using SO2 and PM10 average daily density between correlation analysis,the air quality in zibo city environment for further control government provide certain basis.

Total nitrogen(TN),total phosphorus(TP),potassium permanganate index(CODMn),transparency(SD) and chlorophyll five items were selected to evaluate the eutrophication degree of Mata Lake by using index method of comprehensive nutrition evaluation.The pollution status of Mata Lake were obtained by drawing figures to compare the annual average values of major indexes,including total nitrogen,ammonia nitrogen,chemical oxygen demand(COD),5 days biological oxygen demand(BOD) and potassium permanganate index.The reasons for the eutrophication of Mata Lake was studied based on analysis and measures to improve the eutrophication were put forward.

随着城市化的快速发展,中国社会民众的住房痛苦却日益严重,这体现出对住房公平性的呼唤。住房公积金制度是中国城镇居民住房保障体系的重要内容之一,但是,目前中国很多城市的住房公积金制度在覆盖面、存贷关系、缴存额度上存在明显的社会不公。试图在公平视角下对这一现象做出分析,并尝试提出若干对策建议。

山东淄博作为老工业城市,以科学发展观为指导,把环保工作摆在作为正处于转型升级阶段的老工业城市,环保工作任务艰巨,只有坚持以科学发展观为指导,牢记环境保护是全市经济社会发展的“命门”,以环保为重要的倒逼手段,通过狠抓主要污染物减排,推进国家环保模范城创建,实施碧水蓝天行动计划等工作,才能切实改善环境质量,保障人民群众身体健康,实现内涵式发展的目标.

In addition to the water resources' uneven distribution over time and space,severe water pollution and unreasonable exploitation resulted in the relative shortage of water resources and serious conflicts between water-supply and water-demand.This paper puts forward some new ideas on the exploitation and utilization of water resources in China attempting to correct the unreasonable modes of water utilization to benefit the whole society.