A superior electrochemical biosensor was designed for the determination of UO22+ in aqueous solution by integration of DNAzyme and DNA-modified gold nanoparticle (DNA-AuNP) network structure. Key features of this method include UO22+ inducing the cleavage of the DNAzyme and signal amplification of DNA-AuNP network structure. In this electrochemical method, the DNA-AuNP network structure can be effectively modified on the surface of gold electrode and then employed as an ideal signal amplification unit to generate amplified electrochemical response by inserting a large amount of electrochemically active indicator methylene blue (MB). In the presence of UO22+, the specific sites on DNA-AuNP network structure can be cleaved by UO22+, releasing the DNA-AuNP network structure with detectable reduction of electrochemical response intensity. The electrochemical response intensity is related to the concentration of UO22+. The logarithm of electrochemical response intensity and UO22+ concentration showed a wide linear range of 10~100 pM, and the detection limit reached 8.1 pM (S/N = 3). This method is successfully used for determination of UO22+ in water samples.

A biosorbent, 4-sulfonylcalix[6]arene modified Fe3O4@Aspergillus Niger (MFSC), was successfully prepared through a two-step route for the effective removal of uranium (U(VI)) from aqueous solutions with high selectivity. The structure of MFSC was characterized by FT-IR, SEM, XRD, TGA and VSM, respectively. The impacts of various experimental parameters were investigated in detail. The results indicated that the biosorption of U(VI) on MFSC was mainly attributed to the electrostatic attraction (91% within 8 hours for U(VI)). The adsorption kinetics and adsorption isotherm of U(VI) were found to follow the pseudo second-order model and to be fitted by the Langmuir model, respectively. The thermodynamic parameters revealed that the adsorption process was spontaneous and endothermic. The findings herein highlight the MFSC with high ability for removal of U(VI) from aqueous solutions.

制备了4-磺酰杯[6]芳烃修饰的Fe3O4磁性纳米粒子(MFS),利用MFS对铀废液进行吸附.其结构经红外(IR)、扫描电镜(SEM)、振动样品磁强计(VSM)、热综合分析仪(TGA)等进行表征,表明MFS的粒径在10～40 nm,饱和磁化强度为65 emu/g,具有超顺磁性和良好的热稳定性.在100 mL铀初始浓度2.5 mg/L,pH为6,吸附时间3.5 h,吸附剂用量0.015 g,温度50℃的条件下,最大吸附率为94.39％.由此可见,MFS是一种在放射性污染防控和铀资源循环利用领域极具应用前景的功能化磁性纳米材料.

Object:To establish a gas chromatographic method for the determination of nitenpyram in Lonicerae Ja-ponicae Flos.Methods:The extraction rate of different solvents and the purification effect of solid phase extraction material on the interference matrix in the sample were eluted with methanol.The DB-1701 quartz capillary column and GC-ECD were used to determine nitenpyram in Lonicerae Japonicae Flos.Results:The method showed a good linearity (r＝0.999 3)in the range of 0.005～0.5 mg·L－1.The recoveries were 79.14% ～98.37% with addition of 0.05,0.1 and 0.2 mg·kg－1respectively, and with RSDs of 3.66% ～4.39%.The detection limit of the method is 0.006 mg·kg－1.Conclusion:The established detec-tion method has the advantages of high sensitivity,high accuracy and good linearity,in line with the requirements of pesticide residue analysis,and can be used for the determination of nitenpyram residues in Lonicerae Japonicae Flos.

In this experiment,fulvic acid and coptic chinensis antidote were the bulk material,and 150 broilers were randomly divided into blank group,the antibiotic group,fulvic acid group,coptic chinensis antidote powder group,mixed group Ⅰ,group Ⅱand mixed group Ⅲ.By examining the structure of the chicken gut (intestinal villus height,crypt depth),bacteria (Bifidobacterium,Lactobacillus,Escherichia coli),number of digestive enzymes (protease,lipase,amylase) activity,as well as pH value and other indicators,effect of joint function of coptic chinensis antidote and fulvic acid powder on bowel function were explored.The results showed that compared with the blank group,the mixed group Ⅱcould increase the number of intestinal lactobacilli and the activity of amylase was 6.83％(P＜0.05) and 15.23％(P＜0.05) up respectively.Compared with the blank group,protease activity and the crypt depth in mixed group Ⅰ was increased by 77.94％(P＜0.01) and 15.84％ (P＜0.01).Compared with the antibiotics group,the height of intestinal villus in mixed group Ⅰ was increased by 32.74％(P＜0.01).Compared with mixed group Ⅲ,the V/C value in mixed group Ⅱ was 33.06％(P＜0.01) up.The results showed appropriate amount of fulvic acid and coptic chinensis antidote in broiler diets can significantly improve broiler digestive enzyme activity,broiler intestinal mucosa structure and composition of flora for better broiler performance.

The 4-sulfonylcalix[6]arene modified Fe3O4 (MFS) was characterized by FT-IR, SEM, VSM, TGA, etc., which showed that its saturation magnetization was 64.99 emu g−1 with the particle size 10–40 nm. The maximum adsorption efficiency by MFS for 2.5 mg L−1 U(VI) solution amounted to 94.39%, which was higher than that by Fe3O4 (65.22%) under its optimum adsorption conditions. The adsorption of MFS and Fe3O4 were both followed the pseudo-second order model and the Langmuir isotherm model. The Gibbs free energy change and enthalpy change revealed that the adsorption of U(VI) by MFS was a spontaneous and endothermic process.

A novel DNA electrochemical biofilm electrode was fabricated using Sulfo-SMCC as the heterobifunctional coupling agent which made the sulfhydryl-modified DNAzyme,covalently immobilized on the aminated mesoporous silica nanoparticles (MSN) loaded with the electrochemical indicator methylene blue (MB),and then mixed with chitosan (CTS) dropping applied to the carbon paste electrodes.The preparation process of the biofilm electrode was characterized by cyclic voltammetry (CV),electrochemical impedance spectroscopy (EIS) and so on.The results showed that the DNAzyme based on thiol-modified was successfully immobilized on the silica nanomaterials,and the prepared electrode material formed a stable film on the surface of the carbon paste electrode.

The authors describe an ultrasensitive amperometric enzymatic assay for uranyl ion. It is based on the use of mesoporous silica nanoparticles (mesoSiNPs) loaded with Methylene Blue (MB) and functionalized with an UO2(II)-dependent DNAzyme. The electroactive label MB was sealed in the inner pores of the mesoSiNPs along with double stranded DNA (containing the DNAzyme and the substrate strand). In the presence of UO2(II), the DNAzyme is actived to cleave the substrate strands. This leads to the cleavage of the caps and the release of MB from the mesoSiNPs. The amount of released MB depends on the concentration of UO2(II) and can be determined amperometrically, best at a working voltage of −0.25 V (vs SCE), by using a chitosan coated carbon paste electrode. Response is linear in the 20 pM to 0.1 nM UO2(II) concentration range, and the detection limit is as low as 0.15 pM. Recoveries from spiked samples varied from 91.3 to 99.4%. The assay is highly specific, selective, and not interfered by other metal ions.

利用3-(2,3-环氧丙氧)丙基三甲氧基硅烷(EPPTMS)与正硅酸乙酯在Fe3O4磁性纳米粒子(MN)表面发生共聚反应,制得表面富有含氧活性基团的复合磁性纳米吸附剂(EPPTMS-MN),研究EPPTMS-MN对铀的吸附性能及其机理,结果表明,EPPTMS-MN是良好的铀吸附材料.

利用溶胶-凝胶法制备了有序介孔材料MCM-41,并用四乙烯五胺对其进行修饰,制备了多氨基改性的介孔氧化硅吸附剂.采用低温氮吸附(BET)、扫描电镜(SEM)、红外光谱(IR)、X射线粉末衍射(XRD)对材料进行表征.BET比表面积为1052 cm2/g,孔容为1.194 mL/g,平均孔径为3.86 nm.当pH =5.2,吸附时间30 min,初始浓度120mg/L时,吸附剂对铀酰离子的吸附达到454 mg/g.

利用溶胶-凝胶法合成MCM-41,并用γ-缩水甘油醚氧丙基三甲氧基硅烷(KH560)进行环氧基修饰,制备了未经修饰改性和环氧基功能化的两种MCM-41材料.通过电镜、XRD、红外(IR)、氮气吸附脱附等方法对两种材料的性能进行比较研究,并利用这两种材料设计了pH控制的布洛芬吸附-释放系统.结果表明,改性前后的MCM-41均保持了良好的孔道结构,MCM-41释放迅速,且释放量多,8h达到饱和,而环氧基改性后的MCM-41能够起到缓慢释放的效果,为释放总量65％左右,低于前者.

Magnetic molecularly imprinted polymer with the specific identification function was synthesized in sol‐gel process with S‐ibuprofen as template molecule ,APTES as functional monomer ,and ethyl orthosilicate as cross linking agent .The structure of magnetic molecularly imprinted polymer of S‐ibuprofen was characterized by infrared absorption spectrum (IR) , scanning electron microscopy (SEM ) and the X‐ray diffraction (XRD ) ,respectively .T he re‐search of adsorption and separation performance of S‐ibuprofen magnetic molecularly imprinted membrane was implemented by ultraviolet (UV) and high performance liquid chromatography (HPLC) with chiral mobile phase .The results indicated that the magnetic molecularly imprin‐ted polymers have good magnetic response and achieve rapid separation in addition to magnetic field;S‐ibuprofen molecularly imprinted composite membrane has a good selection to S‐ibu‐profen ,the binding capacity achieve to 68 3. mg/g ;every 100 mg film can increase the degree of separation of ibuprofen by 2 1.% .

Medical chemistry is the chemistry and medicine related content integration close together, is an important basic course for medical students. The teaching content of basic chemistry course for medical students, dififcult to understand. Application of network method, design of medical chemistry network course, scientiifc learning module, the extensive use of multimedia lfash animation, text, graphics, audio and video and so on, guide the student to carry on the exploration and discovery learning, stimulate students interest in learning, and achieved good teaching effect, improve the quality of teaching to promote the development of teaching reform.

《医学基础化学》是临床医学专业学生的一门重要基础课程。该文通过对照教育部《本科医学教育标准—临床医学专业（试行）》标准,结合临床医学专业认证实践工作,从医学基础化学课程教学的师资队伍建设、教学内容、教学方法与手段、教学效果评价及实践教学等方面提出了几点思考,以提高学生培养质量。

The geometric structure optimization, frequency analysis, infrared spectroscopy, natural charge population, the natural bond orbital (NBO) analysis, the molecular and the frontier orbital energy are calculated about glucose, pyran-fructose, furfuran-fructose and inulin molecule by using density functional theory (DFT) method in B3LYP on the 6-311+g(d,p) basis set level.It is shown the stability order is: furfuran-fructose > pyran-fructose > glucose > inulin.We find that inulin's chemical potential is maximum, the chemical hardness is minimum and the electrophilicity index is maximum using the concept DFT method, which could account for it's pharmacological activity.The Fukui function scanning has shown the C2 atom in inulin molecule has strong electron-losing ability and it is the active center in the molecule.The EBDE calculation has shown the position of O 2--H is the most easily broken off and the bond energy is only 94.65 kcal mol -1 , which is far less than the adiabatic ionization potential value, all of that could be a reasonable theoretical interpretation for the pharmacological activity of inulin.

A novel highly sensitive and specific electrochemical biosensor for detecting uranium based on specific Deoxyribozymes and gold nanoparticles (AuNPs) is reported. In this work, AuNPs provide excellent electrochemical signal transduction and a large surface area for immobilising numerous Deoxyribozymes, so a low detection limit of 3.24ngL(-1) uranium and a good linear relationship over the range 5.94-35.1ngL(-1) (r=0.994) were obtained. The proposed biosensor presents high specificity and selectivity for uranium and is not affected by other metal ions. Thus, the biosensor protocol offers good selectivity, rapid speed and operational convenience for detection uranium in liquid waste.

随着中外教育交流的日益频繁和教育国际化趋势的逐渐增强,研究生英语教学之外的专业英语交流及应用越来越受到人们的重视.研究生是否具备专业英语素养和专业英语应用能力已成为评判复合型研究生人才的一项重要指标.素质教育背景下的研究生双语教学体现了时代发展的需求,是实现国际化人才培养目标的有效途径.小组讨论式教学法是目前世界上许多国家普遍采用的一种富有创意的教学理论与方法.本文试以所在地方高校研究生课程化学与生物传感器教学实践为例,结合小组讨论式教学法,探索地方高校研究生双语教学改革的思路与实践模式.

A novel and highly sensitive electrochemical DNAzymes biosensor was fabricated using Au nanoparticles (AuNPs) immobilized on the surface of Au electrode that had been previously modified with self-assembled monolayers of 1,6-hexanedithiol. Different modified electrodes were prepared and characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The AuNPs were found to have a large surface area to anchor a large number of negatively charged phosphate backbones of DNAzymes, which further absorbed the electroactive indicator of hexaammineruthenium(III) ([Ru(NH3)(6)](3+)) to amplify the electrochemical signal. In the presence of target molecules, a large amount of DNA partly associated with [Ru(NH3)(6)](3+) were removed from the electrode surface, leading to a significant decrease in peak current. Differential pulse voltammetry signals of [Ru(NH3)(6)](3+) provided quantitative measures of the concentrations of uranyl ion (UO22+), with linear calibration ranging from 13 pM to 0.15 nM and a detection limit of 5 pM. The presence of other metal ions did not affect the detection of UO22+, which indicated the high specificity of UO22+. Therefore, a new electrochemical DNAzymes sensor was designed with specific DNAzymes and AuNPs as immobilization platform and signal amplifier.

阻碍高校内涵发展有效实施的一个主要因素是高校内部多元价值文化的集合与冲突.针对当前高等教育教书与育人两极分化的现象,提出了一种内外兼顾、多层次、多对象的全面竞争式内涵发展模式.探讨了高校在作为个体与整体的角色互换过程中,其内部教师与教育工作者之间的内涵发展关系,进一步探索了教育工作者资格与教师资格的并存制度,突出以育人为本的高校教师主体地位和高校教育工作者的服务职能.逐步树立以服务求发展,以奉献求效益的发展理念,多措并举推动地方高校的错位发展.

Magnetic carboxymethylcellulose (CMC/Fe3O4) was used as a framework adsorption material to remove uranium ions from aqueous solutions. Carboxyl functional groups were grafted onto the CMC/Fe3O4. The maximum adsorption capacity of the magnetic composite toward U(VI) was 122.48 mg/g. Kinetic and thermodynamic parameters of the adsorption process were estimated. The pseudo-second-order model was more suitable and it proved to be an endothermic and spontaneous process. The Langmuir and Freundlich models were applied to evaluate the adsorption isotherm. The data matched well with Langmuir model after equilibrium was reached and with Freundlich model before equilibrium was reached. (C) 2014 Elsevier B.V. All rights reserved.