Four pairs of primers were designed according to the sequences of invA gene of Salmonella,MapA gene of Campylobacter jejuni,hlyA gene of Listeria monocytohenes and O gene cluster of Escherichia coli O157:H7. After optimization of conditions,a multiplex-PCR assay was established for rapid detection of the 4 common pathogens in food.Results showed that the multiplex PCR assay could amplify four specific bands and more than 20% difference in length existed between any two of them,and the sensitivity of the PCR system was at least 100 CFU mixed templates of the 4pathogens. The multiplex-PCR assay was rapid,accurate and specific in operation and could be used in food inspection.

<正>近年来,广西多个养鸡场发生多发性关节炎病例,给养殖场带来了较大的经济损失。2010年6月广西某三黄鸡养殖场鸡群发生了一起以局部关节肿胀和跛行为主要表现的病例。经流行病学调查、临床

Objective: To understand the situation of Salmonella contamination and its serotype distribution in food products of animal origin in Nanning,Guangxi during 2009—2010 and to provide useful information for monitoring and control for foodborne diseases.Methods: The isolation and serotyping of Salmonella isolates were carried out according to the method of Microbiological examination of Food Hygiene: Examination of Salmonella(GB/T 4789.4—2008).Results: Detectable levels of S.gallinarum,S.typhimurium,S.javiana etc in diseased poultry and S.derby,S.gallinarum,S.typhi etc in meat products derived from poultry and livestock were found.Conclusion: More Salmonella serotypes more extensively spread in food products of animal origin,indicating diverse contamination pathways.Consequently,Salmonella contamination risks can be effectively reduced only by strengthening hygiene supervision throughout the whole food chain from production to distribution.

<正>沙门氏菌病是指由沙门氏菌属细菌所引起的人类、畜禽等动物各种疾病的总称,是细菌性人畜共患病之一,包括肠炎沙门氏菌等众多血清型在内的沙门氏菌则是主要的食源性病原菌之一,在公共卫生学占有重要地位。沙门氏菌病是贯穿畜禽养

引起肉鸡关节炎和多发性关节炎(polyarthritis)的病原体主要有:禽呼肠孤病毒(avian reovirus, ARV),沙门氏菌包括鸡白痢沙门氏菌(S.pullorum)、鸡伤寒沙门氏菌(S.gallinarum)和副伤寒(paratyphoid)沙门氏菌,滑膜支原体(Mycolpasma synoviae, MS)、金黄色葡萄球菌(Staphylococcus aureus),大肠杆菌(E.coli)以及多杀性巴氏杆菌及丹毒杆菌[1].

<正>肉鸡的多发性关节炎是一种重要的传染病,主要有以下2种类型:由呼肠孤病毒(avianreovirus,ARV)引起的肉鸡病毒性关节炎,由沙门菌(Salmonella)、大肠杆菌(E.coli)、金黄色葡萄球菌(Staphylcoccus aureus)、多杀巴氏杆菌(Pasteurella multocida)等引起的细菌性关节炎[1]。