本试验研究了不同浓度的大豆苷(daidzin)和染料木苷(genistin)对山羊乳腺上皮细胞(mammary gland epithelial cells,MEC)增殖的影响,主要采用MTT法研究不同浓度的大豆苷和染料木苷刺激影响细胞增殖的状况.结果表明,2种苷纳克级别浓度组较微克级别浓度组对细胞增殖的影响更明显;处理细胞48 h较24和72 h对细胞增殖的影响更明显;10 ng/mL大豆苷和10、100 ng/mL染料木苷均能显著促进山羊MEC增殖(P＜0.05).

本文综述了矿物元素、维生素、中草药和牧草对猪肉品质的影响,为其在饲料中的合理应用提供参考.

In this study,we selected tissue culture method to isolation chicken intestinal epithelial cells(IEC),using curettage,phase contrast digest and adherence methods to purify cells,with 0.05% Trypsin to digest and passage,immunocytochemical method to identify intestinal epithelial cells.The results showed that hadro-activity intestinal epithelial cells could be dissociated by tissue culture method,and obtain purified intestine epithelium.Morphology and immunocytochemical method showed that intestinal epithelial cells surface antigen demonstrate masculine,and purified intestine epithelium could be passaged in vitro.

牧草次生代谢产物不仅具有抗氧化、抗病毒、消炎等生物活性,也能提高鸡的产蛋率,家畜的免疫力,改善肉品质.牧草次生代谢产物是近年来的研究热点,本文从牧草次生代谢产物的生物活性和在动物中的利用方面综述了其研究进展,从而为牧草次生代谢产物的进一步研究利用打下理论基础.

本试验采用组织块培养法获得原代小肠上皮细胞,联合刮除法、相差消化法纯化原代细胞,用0.05%的胰蛋白酶进行消化传代,对山羊小肠上皮细胞进行了原代分离培养、传代、纯化及鉴定,同时以山羊小肠上皮细胞为模型,体外研究山羊小肠上皮细胞对氨基酸的代谢作用.免疫组化鉴定结果表明所分离纯化的细胞为小肠上皮细胞;组氨酸、精氨酸、苏氨酸和亮氨酸是小肠上皮细胞快速利用的氨基酸,在72 h内快速下降;天冬氨酸、谷氨酸、丙氨酸、脯氨酸是生成的氨基酸,在72 h内有所积累;其它氨基酸呈现平缓的被消耗状态.

Saccharicterpenin is a green additive with non-pollution,plain and no drug residue.It can improve poultry production performance and enhance the immune functions.This paper outlines Saccharicterpenin benefits not only from the nutritional aspects but also from bioactivity,and pave the way for the theoretical basis for poultry industry.

采用限制性内切酶EcoRⅠ和SalⅠ双酶切质粒pBABE-hygro-hTERT,获取端粒酶催化亚基(hTERT),将其定向克隆至载体pEGFP-N1,构建重组的荧光真核表达载体pEGFP-N1-hTERT.经双酶切、测序及转染成纤维细胞鉴定均证实hTERT基因已插入pEGFP-N1载体中,成功构建了荧光真核表达载体pEGFP-N1-hTERT.构建的pEGFP-N1-hTERT,为进一步筛选目的细胞和建立永生化细胞系奠定了基础.

By the technology of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),genetic polymorphism of exon 4 and exon 5 of κ-casein gene in southern Chinese Holstein cattle were studied.The results showed as follows: exon 4 and exon 5 of κ-CN gene were polymorphic caused by a AC substitution in exon 4(g.13100 AC) and a TC substitution in exon 5(g.5153 AC).The allele frequencies of A,B of exon 4(AA,AB) and exon5(AA,AB,BB)of κ-CN gene were 0.9379,0.0621 and 0.6512,0.3488,repetively.The genotype distribution of the two exons both fitted Hardy-Weinberg Law(P0.05). Exon 4 locus showed low polymorphism(PIC=0.1097) while Exon 5 locus showed normal polymorphism(PIC=0.3511).The heterozygosity and number of effective alleles were 0.1165,1.1318 and 0.4543,1.8324,respectively in exon 4 and exon 5 of κ-CN gene of southern Chinese Holstein cattle.