LncRNAs are defined as non-coding RNAs that are longer than 200 nucleotides in length. The previous studys has shown that lncRNAs played important roles in the regulation of gene expression and were essential in mammalian development and disease processes. Inspired by the observation that lncRNAs are aberrantly expressed in tumors, we extracted RNA from Bladder urothelial carcinoma and matched histologically normal urothelium from each patient and bladder carcinoma cell lines. Then, we reversed transcribed them into cDNA.Last, we investigated the expression patterns of ERIC by the fluorescence quantitative PCR in bladder cancer tissues and cell lines. CRISPR-dCas9-VPR targeting ERIC plasmid was transfected into T24 and 5637 cells, and cells were classified into two groups: negative control (NC) and ERIC overexpression group. MTT assay, transwell assay, and flow cytometry were performed to examine changes in cell proliferation, invasiveness, and apoptosis. We found that the expression of ERIC was down-regulated in bladder urothelial carcinoma compared to matched histologically normal urotheliam. The differences of the expression of this gene were large in the bladder cancer lines. Compared with the negative control group, the ERIC overexpression group showed significantly decreased cell proliferation rate (t = 7.583, p = 0.002; t = 3.283, p = 0.03) and invasiveness (t = 11.538, p < 0.001; t = 8.205, p = 0.01); and increased apoptotic rate (t = −34.083, p < 0.001; t = −14.316, p < 0.001). Our study lays a foundation for further study of its pathogenic mechanism in bladder cancer.

目的:探讨尿动力学检测联合膀胱尿道同步测压对女性膀胱过度活动症(OAB)患者逼尿肌过度活动(DO)状态的评估价值.方法:回顾性分析2017年6月-2019年6月本院收治的80例女性OAB患者的病例资料,并将其作为观察组,选取同期于本院健康体检中心进行体检的50例健康女性志愿者为健康组.两组均进行了尿动力学检测和膀胱尿道同步测压检查,比较两组的初始尿意容量(FDV)、残余尿量(PVR)、最大尿流率(MFR).比较两组的最大尿道压(MUP)和最大尿道闭合压(MUCP),分析其在OAB中的表达特征.根据DO发生情况将80例女性OAB患者分为DO组和普通OAB组,比较两组尿动力学相关指标和膀胱尿道同步测压相关指标,通过Pearson直线相关性分析比较上述指标分别与DO发生情况的相关性.结果:观察组的FDV、MFR、MUP和MUCP水平均低于健康组,PVR水平高于健康组,差异均有统计学意义(P<0.05).DO组的FDV、MFR、MUP和MUCP水平均低于普通OAB组,PVR水平高于普通OAB组,差异均有统计学意义(P<0.05).Pearson直线相关性分析结果显示,DO发生率与MFR、MUP、MUCP水平均呈明显负相关性(P<0.05),与PVR水平呈明显正相关性(P<0.05).结论:尿动力学检测联合膀胱尿道同步测压能够评估女性OAB患者的DO状态,具有一定的临床意义.

目的 研究超微通道经皮肾镜取石术和输尿管软镜碎石术治疗1～2cm肾下盏结石的效果比较.方法 选取2018年4月～2019年10月在我院进行碎石术的1～2cm肾下盏结石患者100例,使用随机数字表法将其分为A组和B组,每组50例.A组使用输尿管软镜钬激光碎石术(RIRS)治疗,B组使用超微通道经皮肾镜取石术(SMP)治疗.对比两组的结石清除总有效率、手术情况、手术并发症情况.结果 B组的结石清除总有效率(96％)高于A组(76％),B组的手术用时短于A组,血红蛋白低于A组,手术出血量多于A组(P<0.05),两组患者的不良反应总发生率比较,差异无统计学意义(P>0.05).结论 超微通道经皮肾镜取石术相比输尿管软镜碎石术治疗1～2cm肾下盏结石的效果显著,经过处理后出血情况得到有效控制,安全性相差不大.

目的:探讨三黄清淋汤联合针刺治疗慢性前列腺炎的效果.方法:选取2018年3月—2019年12月在我院诊治的60例慢性前列腺炎患者作为研究对象,分为对照组(30例,给予三黄清淋汤治疗)和观察组(30例,在对照组基础上增加针刺治疗).观察两组临床疗效、炎性指标及慢性前列腺炎症状指数(NIH-CPSI).结果:治疗后,观察组总有效率为93.33％(28/30),高于对照组的76.67％(23/30)(P<0.05);观察组炎症因子(NSE、IL-6、TNF-α)水平明显低于对照组(P<0.05);观察组NIH-CPSI评分低于对照组(P<0.05).结论:慢性前列腺炎应用三黄清淋汤联合针刺治疗疗效显著,降低炎性反应,缓解临床症状,值得推广.

Objective To summarize the clinical data of penile fracture,and to improve the level of the diagnosis and treatment of penile fracture.Methods Retrospective analysis was performed on the clinical data of 12 cases of penile fracture,including the symptoms and signs,examination methods,treatment and prognosis.Results All the patients presented penile swelling and ecchymosis.Ten patients reported hearing snap sound.Three patients were verified by ultrasongraphy to be with cavernosum tunica tear.All the patients were successfully treated by operation,without erect dysfunction,peyronie's disease and pain during erection after 3 months to 5 years of follow-up.The mean IIEF score was 22.6.Conclusion Penile fracture has typical history and clinical manifestations.Ultrasound has a certain diagnostic value.Surgical repair was effective,safe and reliable for penile erectile function recovery after the break,and timely surgical repair with no sexual dysfunction.

>2008年12月至2011年8月,我们对24例复杂性肾结石行二期经皮肾镜取石手术(percutaneous nephrolithotomy,PCNL),疗效满意。现报告如下。对象与方法本组24例。行24 F标准通道经PCNL或经皮肾穿刺造瘘置管引流术,常规留置20 F肾造瘘管。术后3 d行B超、KUB或CT检查:残留结石最大径25～42 mm,残留结石表面积>300 mm。其中10例为术中穿刺、扩张或碎石操作损伤出血较多导致视野