目的 探讨玄驹胶囊对去势模型动物睡眠的影响.方法 采用摘除双侧卵巢的方法分别建立大、小鼠去势模型.将模型动物随机分为模型组、更年安胶囊组和玄驹胶囊高、中、低剂量组,另设假手术组作为对照.通过观察小鼠自主活动、戊巴比妥钠阈下剂量催眠实验、延长戊巴比妥钠睡眠时间实验来评价小鼠睡眠情况,并检测小鼠血清褪黑素(MT)水平.大鼠实验则检测各组大鼠直肠温度、血清性腺激素雌激素(E2)、卵泡刺激素(FSH)、黄体生成素(LH)水平;试剂盒检测下丘脑匀浆液神经递质五羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)、五羟吲哚乙酸(5-HIAA)水平;计算肝脏、脾脏、子宫、胸腺、肾上腺脏器系数.结果 与模型组比较,玄驹胶囊中、低剂量组小鼠入睡率增加(P<0.05,P<0.01),高剂量组小鼠睡眠潜伏期缩短(P<0.01),睡眠时间延长(P<0.01),中剂量组睡眠潜伏期缩短(P<0.05),各给药组对小鼠自主活动无明显影响(P>0.05);玄驹胶囊高剂量组大鼠NE水平升高(P<0.05),5-HIAA水平及胸腺系数降低(P<0.05).结论 玄驹胶囊具有改善去势模型动物睡眠的作用.

目的 观察参元益气活血胶囊对大鼠心肌缺血再灌注损伤(MIRI)的影响.方法 SD大鼠分为假手术组、模型组、复方丹参片1.26g/kg(对照组)和参元益气活血胶囊低(1.08g/kg)、中(2.16g/kg)、高(4.31g/kg)剂量组.采用大鼠结扎冠状动脉左前降支30 min,再灌注120 min制备MIRI模型,测定血清AST、LDH、CK-MB、CK、Na+-K+-ATPase、Ca2+-Mg2+-ATPase、PGI2、TX-A2、SOD、MDA水平.结果 与模型组比较,参元益气活血胶囊高剂量组可明显抑制血清中AST、CK、LDH、TX-A2、MDA 的释放,提高 Na+-K+-ATPase、Ca2+-Mg2+-ATPase、PGI2、SOD 活力(P＜0.01,P＜0.05);参元益气活血胶囊中剂量组可明显抑制血清中CK、LDH的释放,提高SOD活力(P＜0.01,P＜0.05).结论 参元益气活血胶囊对MIRI大鼠心肌具有保护作用.

目的 研究调和肝脾方改善睡眠的时效关系及作用机制.方法 实验一:将体质量20～22 g ICR雄性小鼠90只随机分为空白对照组10只、调和肝脾方中剂量组40只和调和肝脾方高剂量组40只,调和肝脾方中、高剂量组分别给予相应剂量调和肝脾方灌胃,空白对照组给予同体积蒸馏水灌胃,均每日1次.调和肝脾方中、高剂量组分别于干预3,5,7,9 d给药1 h后各随机取10只,分别腹腔注射40 mg/kg(阈剂量)的戊巴比妥钠溶液,记录小鼠的睡眠潜伏期以及睡眠时间.实验二:分组及干预方式同实验一,调和肝脾方中、高剂量组分别于干预3,5,7,9 d给药30 min后各随机取10只,分别腹腔注射30 mg/kg(阈下剂量)戊巴比妥钠溶液,计算入睡率.实验三:将体质量20～22 g ICR雄性小鼠30只随机分为空白对照组、调和肝脾方中剂量组、调和肝脾方高剂量组,每组10只.调和肝脾方中、高剂量组分别给予相应剂量调和肝脾方灌胃,空白对照组给予同体积蒸馏水灌胃,均每日1次,连续灌胃7 d,于末次灌胃30 min后,将各组小鼠直接断头处死,检测皮质、下丘脑、海马体中多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)、一氧化氮(NO)、一氧化氮合酶(NOS)含量.结果 调和肝脾方中、高剂量组灌胃5,7,9 d的睡眠潜伏期均显著短于空白对照组(P均<0.05);调和肝脾方中剂量组灌胃7,9 d和调和肝脾方高剂量组灌胃5,7,9 d的睡眠时间均显著长于空白对照组(P均<0.05).调和肝脾方中剂量组灌胃5,7,9 d和调和肝脾方高剂量组灌胃3,5,7,9 d的小鼠入睡率均显著高于空白对照组(P均<0.05).调和肝脾方中剂量组海马体DA、NE含量和皮质NO、DA、NE、NOS含量均显著低于空白对照组(P均<0.05);调和肝脾方高剂量组皮质NOS、NE含量和丘脑NO含量均显著低于空白对照组(P均<0.05).结论 调和肝脾方改善睡眠的作用呈现一定的时效关系,其给药5d起效,最佳给药时间为7d.调和肝脾方改善睡眠的作用与调节中枢神经递质DA、NE及NO含量相关.

目的 研究中药复方制剂调和肝脾方对失眠作用的影响.方法 通过记录小鼠睡眠时间及入睡率等来观察调和肝脾方对小鼠戊巴比妥钠阈剂量及阈下剂量作用下睡眠的影响;通过注射对氯苯丙氨酸(PCPA)诱导失眠大鼠模型,并观察给药后对5-羟色胺(5-HT)含量的影响.结果 调和肝脾方能显著延长阈剂量小鼠的睡眠时间(P＜0.01),对小鼠睡眠持续时间具有延长作用(P＜0.01);能增加阈下剂量小鼠的入睡率(P＜0.01);能显著升高失眠大鼠脑内5-HT含量(P＜0.05).结论 调和肝脾方治疗失眠有一定疗效.

Objective To observe the effects of lotion for promoting blood circulation and removing blood stasis so as to provide experimental evidence for the clinical application. Methods The rat models with acute blood stasis were applied with the lotion for promoting blood circulation and removing blood stasis for prevention,its influence on the whole blood viscosity and plasma viscosity were detected; the rat models with microcirculation dysfunction were applied with the lotion for prevention,and the improvement of microcirculation in auricle were observed; the rat models with pain were applied for prevention with the lotion,and its analgesic effect was observed. Results Compared with the acute blood stasis model group,the whole blood viscosity in the lotion group with the dosage of 0. 32,0. 64 g / m L was reduced significantly( P 0. 05,P 0. 01); compared with the microcirculation dysfunction model group,the microcirculation of auricle in lotion group with the dosage of 0. 40,0. 80 g / m L was improved( P 0. 05); a certain analgesic effect for pain caused by the chemical and heat stimulation was shown in the lotion group with the dosage of 0. 40,0. 80 g / m L( P 0. 05). Conclusion Lotion for promoting blood circulation and removing blood stasis has certain effect in promoting blood circulation and removing blood stasis and also has an analgesic action.

目的研究苏合香包合物的药理作用。方法观察苏合香包合物对乙醇及亚硝酸钠致小鼠记忆障碍模型的影响；采用注射冰醋酸制造小鼠疼痛模型，观察苏合香包合物的镇痛作用；观察苏合香包合物对戊巴比妥钠致小鼠睡眠的影响作用。结果苏合香包合物能显著降低乙醇及亚硝酸钠致小鼠记忆障碍平均潜伏期；对化学刺激引起的疼痛有一定的镇痛作用；能显著延长戊巴比妥钠所致小鼠的睡眠潜伏期和缩短睡眠持续时间。结论苏合香包合物具有一定的改善小鼠记忆障碍、镇痛、催醒作用。

Objective To observe the intervention of Ershuping( ESP) Keli on rat model with sexual precocity. Methods 32 SD rats were randomly divided into a normal control group,a model control group,a high-dosage ESP group and a low-dosage ESP group according to the weight. The rat models with true sexual precocity were made by subcutaneously injecting N-Methyl-DL-aspartic acid. At the same time,the high-dosage and low-dosage ESP groups received 35. 42 and 17. 71 g / kg Ershuping Keli respectively with intragastric administration once a day. The normal control group and model control group were given deionized water with intragastric administration. The vaginal opening,the appearing time of the first Diestrus,sex organ index,and the contents of serum E2,LH and FSH were compared. Results Vaginal opening and the first Diestrus showed significantly delayed,uterus and ovary index decreased in two ESP groups,and the difference had statistic significance( P 0. 05); serum sexual hormones also showed a certain decreased trend,but the difference had no statistic significance( P 0. 05) compared with the model group. Conclusion Ershuping Keli has certain curative effect on sexual precocity in rat model.

Objective To observe the protective effect of five constituents of bee collected from rape pollen on rats with hepatic injury caused by D-galactosamine hydrochloride. Methods The experiment was divided into seven groups: control group,D-galactosamine hydrochloride model group,pollen extract A,B,C,D,E groups. After successive administration for 10 days,these groups except the blank control group received intraperitoneal injection of 10% D-galactosamine hydrochloride solution to induce a liver injury model. The activity of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) in serum and the level of interleukin-6(IL-6) and tumor necrosis factor α(TNF-α) in liver tissue were measured. Results Compared with the model group,pollen extract A significantly reduced the activity of AST,ALP and the level of IL-6 and TNF-α,while pollen extract B,E could significantly reduce the activity of ALT,AST,ALP and the level of TNF-α. Conclusion Pollen extract A,B,E can reduce the biochemical parameters of D-galactosamine-induced liver injury and have a certain protective effect on the liver of rats. The mechanism may be related to its anti-inflammatory function as shown by the reduced level of IL-6 and TNF-α in liver tissue.

Objective To study the effect of Dandixiaoyin pill(DDXY) on psoriasis-like animal model in order to provide experimental basis for clinical application.Methods The model of vaginal epithelium mitosis of mouse was established by diethylstilbestrol to observe the effect of DDXY on mitosis in vaginal epithelium cells of mouse;meanwhile,the mouse tail scale epidermis model was established to investigate the ability of DDXY in promoting the formation of stratum granulosum in mouse tail scale.Results The high and medium-dose DDXY groups could significantly inhibit vaginal epithelium mitosis of mouse in estrogen phase(P<0.05,P<0.01);the high-dose DDXY group could significantly promote the formation of stratum granulosum in mouse tail scale(P<0.01).Conclusions DDXY has a good effect on psoriasis,and its mechanism is related with inhibiting hyperplasia and improving the parakeratosis of epidermal cells.

目的观察曲直丸的活血化瘀、抗炎、镇痛作用,为临床应用提供实验依据。方法建立大鼠急性血瘀模型,检测曲直丸对其全血黏度、血浆黏度的影响;采用大鼠肉芽肿试验,观测曲直丸的抗炎作用;采用冰醋酸疼痛模型试验和热板试验,观测曲直丸的镇痛作用。结果曲直丸可显著降低大鼠急性血瘀模型全血黏度和血浆黏度,能明显抑制药敏纸片植入法诱导的大鼠肉芽肿的增生,对化学刺激和热刺激引起的疼痛均有一定的镇痛作用。结论曲直丸具有一定的活血化瘀、抗炎、镇痛作用。

目的观察健脑Ⅰ号对大鼠脑缺血再灌注的影响。方法用线栓法手术造成大鼠脑缺血再灌注模型。结果健脑Ⅰ号1.0、0.5g/kg剂量能缓解脑缺血再灌注引起的行为改变以及缩小脑梗死面积。结论健脑Ⅰ号1.0、0.5g/kg对线栓法手术造成大鼠脑缺血再灌注有保护作用。

目的:观察抗光敏合剂对急性UVB辐射后BALB/c小鼠皮肤炎症细胞损伤的影响,以探讨抗光敏合剂对皮肤光损伤的作用机理。方法:以300mJ/cm2UVB辐射BALB/c小鼠建立急性光损伤模型,在预防性应用中药抗光敏合剂后,与正常小鼠、预防性应用生理盐水及羟氯喹小鼠进行平行对照研究。HE染色法观察炎症变化程度,并以病理图片处理系统观察小鼠表皮厚度。结果:动物实验研究结果显示,中药抗光敏合剂可以缓解急性UVB辐射损伤造成的细胞炎症反应,保护角质形成细胞,发挥对急性光损伤保护的作用机制。结论:抗光敏合剂治疗可明显缓解紫外线引起的表皮角质形成细胞的炎性损害,从而发挥其治疗作用。

目的观察郁消颗粒的抗抑郁作用。方法采用大鼠强迫游泳、小鼠悬尾及利血平拮抗实验动物模型,观察郁消颗粒对大鼠游泳绝望时间、小鼠悬尾不动时间,以及对利血平化小鼠眼睑下垂、运动不能发生率及体温下降的影响;观察郁消颗粒对小鼠自主活动的影响,评价郁消颗粒的抗抑郁作用。结果郁消颗粒21.70、10.85g生药/Kg剂量组能缩短大鼠游泳绝望时间;27.90、13.95g生药/Kg剂量组能缩短小鼠悬尾不动时间;27.90、13.95g生药/Kg剂量组能显著对抗利血平所致的小鼠眼睑下垂及小鼠僵直状态发生率,27.90g生药/Kg剂量组可对抗利血平所致小鼠体温下降;未见郁消颗粒对小鼠自主活动的影响。结论郁消颗粒对多种实验性抑郁模型具有明显的疗效。

Objective To investigate the action mechanism of an anti-photosensitivity mixture on skin photodamage. Methods Twenty-eight BLAB/c mice were divided into 4 groups, i.e., normal control group,treatment group, negative and positive control groups; the last three groups were irradiated with a single dose of UVB at 300 mJ/cm2 after 7-day pretreatment with sodium chloride physiological solution, anti-photosensitivity mixture, and hydroxychloroquine, respectively. Twenty-four hours after the irradiation, mice were killed and skin tissue samples were obtained at the irradiated sites. Terminal deoxynucleotidyl transferase （TdT）-mediated dUTP nick-end labeling （TUNEL） and immunohistochemical staining were carried out to detect cell apoptosis,Fas and Caspase-3 protein expressions respectively. Results An increase was observed in the expression level of Fas and Caspase-3 and in the apoptotic index in keratinocytes from UV-irradiated mice compared with unirradiated control mice （all P ＜ 0.01 ）. In comparison with sodium chloride physiological solution, the antiphotosensitivity mixture suppressed the UV irradiation-induced increase in the expression intensity of Fas and Caspase-3 and apoptotic index in keratinocytes （P ＜ 0.05 or 0.01 ）. Conclusions The anti-photosensitivity mixture could alleviate UV-induced inflammatory damage to and apoptosis in epidermal keratinocytes, likely by regulating cell apoptosis and Caspase-3 pathway.

目的研究苏香1号对药物致小鼠记忆障碍模型的作用。方法采用小鼠水迷宫行为实验法,检测苏香1号对戊巴比妥钠、乙醇和亚硝酸钠所致小鼠学习、记忆障碍模型的作用。结果苏香1号28、14mg/kg能显著降低戊巴比妥钠所致的忆获得障碍模型小鼠的潜伏期;苏香1号28、14mg/kg能显著降低乙醇所致记忆再现障碍模型小鼠的潜伏期;苏香1号28mg/kg组能显著降低亚硝酸钠所致的记忆巩固障碍模型小鼠的潜伏期。结论苏香1号能明显改善戊巴比妥钠、乙醇和亚硝酸钠所致的记忆获得障碍、记忆再现障碍以及记忆巩固障碍。

目的观察芪黄清消丸的主要药理作用,为临床应用提供实验依据。方法采用四氧嘧啶造成高血糖大鼠模型,观察芪黄清消丸对高血糖大鼠模型血糖的影响;采用小鼠糖耐量实验,观察芪黄清消丸对葡萄糖的耐受量;采用葡聚糖造成微循环障碍小鼠模型,观察芪黄清消丸对小鼠耳廓微循环的影响。结果芪黄清消丸2.8、1.4g生药/Kg剂量组对四氧嘧啶所致高血糖模型大鼠一定降低血糖作用;3.6、1.8g生药/Kg剂量组可提高小鼠对葡萄糖的耐受量;3.6、1.8g生药/Kg剂量具有改善小鼠耳廓微循环作用。结论芪黄清消丸具有辅助治疗糖尿病的药理作用。

目的观察抗焦胶囊的抗焦虑作用。方法采用戊四唑诱发小鼠惊厥模型,观察抗焦胶囊对小鼠惊厥及死亡潜伏期的影响;采用小鼠期待性焦虑实验,观察抗焦胶囊对焦虑小鼠体温的影响;采用戊巴比妥钠的小鼠睡眠模型,观察抗焦胶囊对小鼠入睡的影响。结果抗焦胶囊2.0g、1.0g、0.5g生药/kg组给药30min可显著延长惊厥潜伏期,2.0g生药/kg组给药60、90、120min能显著延长死亡潜伏期;抗焦胶囊2.0g生药/kg组对焦虑小鼠体温升高有明显抑制作用;抗焦胶囊2.0g生药/kg组能显著增加注射阈剂量戊巴比妥钠小鼠的入睡比率。结论抗焦胶囊对焦虑症有一定缓解作用。

Flavonoid glycosides were prepared from rape bee pollen,and their ability to scavenge DPPH free radicals and reducing power in simulated chemical systems were determined. The antioxidant activity of the extracted flavonoid glycosides was not as strong as that of kaempferol or quercetin. The antioxidant activity of the extracted compounds in vivo against CCl4-induced lipid peroxidation in mice was investigated,and the antioxidant mechanisms were also elucidated. The results showed that the extract could significantly inhibit the generation of MDA in liver,and increase the level of T-AOC in liver and serum. The possible antioxidant mechanism was the generation of flavonoid aglycone with plentiful antioxidant phenolic groups due to the hydrolysis of flavonoid glycosides by in vivo relevant enzymes. Therefore,flavonoid glycosides derived from rape bee pollen had a good protective effect against in vivo oxidative damage.