目的：建立复方金沙利胆颗粒中芍药苷的含量测定方法。方法采用高效液相色谱( HPLC)法对芍药苷进行定量研究；色谱柱条件为Welch Utimate XB-C18色谱柱(4.6 mm ×250 mm,5μm),流动相为：乙腈-纯水(17∶83)；流速：1.0 mL·min-1；检测波长230 nm；柱温：室温(20℃)；进样量：20μL。结果芍药苷在浓度3.92~98μg·mL-1范围内呈现良好的线性关系(r=0.9995),芍药苷平均回收率98.10%,RSD=1.11%(n=6)。结论该法作定量分析简便,重现性好,回收率高,可作为复方金沙利胆颗粒中芍药苷的含量测定方法,可有效控制产品质量。

Objective To establish the determination method for the content of paeoniflorin in Fufang Jinsha Lidan Keli and the standard for the quality control of Fufang Jinsha Lidan Keli.Methods The analysis was performed on a Welch Utimate XB-C18 (4.6 mm ×250 mm, 5μm) column .The mobile phase was acetonitrile and water (17∶83) at a flow rate of 1.0 mL· min -1 .The column temperature was room temperature and the detection wavelength was 230 nm.The injection volume was 20μL.Scutellaria baicalensis Georgi , Cynanchum otophyllum and Fructus aurantii were identified qualititatively by Thin—Layer Chromatography ( TLC).Paeoniflorin in Fufang Jinsha Lidan Keli were determined by HPLC.Results The TLC spots were highly clear without the interference of negative control.The calibration curve for Paeoniflorin was linear ( r=0.9995 ) in the range of 3.92~98.00 μg· mL-1.And the mean recovery was 98.11%.RSD was 1.10%(n=6).Conclusion This method is ac-curate,simple,sensitive and reliable and it can be used for the quality con-trol of Fufang Jinsha Lidan Keli.

Objective To investigate the cholagogic and anti -infla-mmatory effects and acute toxicity of Jinshalidan Granules.Methods The cholagogic effect of Jinshalidan Granules was observed and deter-mined by common bile duct drainage in mouse .The acute inflammatory models was established by xylene -induced ear edema to research the inflammatory effects of Jinshalidan Granules.The acute toxicity and the maximum tolerated dose in mice were also studied.Results Compared with the normal control group , the bile secretion for the mouse and the degree of swelling of mice ear induced by xylene were significantly improvemed in all the 3 different dosages of Jinshalidan Granules.After 14 days, continuous observation ,no mouse was found dead and no signifi-cant toxicity occurred by naked eyes.The maximum tolerated dose of Compound Jinshalidan Granules is 244.8 g · kg -1 ( crude drug ).Conclusion Compound Jinshalidan Granules have obviously effects on the increased the bile secretion and anti -inflammatory ,it is has very low acute toxicity in mice and preliminary proven to be safe .

临床上把非创伤所致的膈肌破裂诊断为自发性膈肌破裂,其发病率极低,临床表现复杂,常合并严重的呼吸循环功能障碍.本病误诊率高,诊治不及时,易导致严重并发症而危及患者生命.我院于2006年2月收治1例自发性膈肌破裂,现总结报告并复习文献如下.

腹部外伤的处理中,最重要的是及时正确诊断有无内脏损伤.十二指由于位置较深,解剖关系复杂,生理机能特殊,发生损伤时,诊断常较困难,易延迟诊断甚至漏诊;手术处理也较困难,并发症多,病死率高.我院1995年至2003年共收治十二指肠外伤破裂17例,现报告如下.

1980年1月～2004年12月,我科共行胆囊切除术1 312例,术后早期出现黄疸11例,占全部病例的0.838%11/1 312).现将其原因分析如下.

重症急性胰腺炎(SAP)起病急骤,发展迅速,并发症多,病死率高.近几年来,随着诊治观念的变化和水平的提高,SAP治愈率有较大提高.现将我院收治的46例SAP的治疗报告如下.

目前,胰腺癌在世界范围内有逐年增加之势,已成为消化道常见的肿瘤之一.由于胰腺癌有恶性程度高,早期确诊率低,手术病死率高,治愈率低等问题,因此,临床工作者必需提高对该病的诊断水平和生存率.本院1994～1998年收治9例胰腺癌,误诊8例,现分析如下.

阑尾炎同时合并机械性肠梗阻临床上少见,且因一方面的临床症状掩盖了另一方面的症状,使诊断或治疗选择发生困难,延误手术时机,造成不良后果.我院1986～1996年收治阑尾炎合并肠梗阻19例,18例以肠梗阻入院,1例以阑尾脓肿入院,术中均证实两者并存,其中死亡1例,现分析如下.